Browsing by Author "Gürtürk, K"

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Detection of Campylobacter Antibodies in Sheep Sera by a Dot-Elisa Using Acid Extracts From C-Fetus Ssp Fetus and C-Jejuni Strains and Comparison With a Complement Fixation Test
(Wiley, 2002) Gürtürk, K; Ekin, IH; Aksakal, A; Solmaz, H
In this study, a dot-enzyme-linked immunosorbent assay (Dot-ELISA) was evaluated in comparison with a complement fixation test (CFT) for the detection of Campylobacter antibodies in sheep sera. Acid glycine extracts (AGE) of both Campylobacter ssp. fetus and Campylobacter jejuni strains that had been isolated from the gall-bladder of slaughtered sheep was used as antigen in both tests. A total of 153 sheep sera from aborted (74) and slaughtered (79) sheep were examined by both Dot-ELISA and CFT. Twenty-two sera showed anti-complementary activity were not suitable for CFT. Of the 22 sera showing anti-complementary activity, two sera were found to be positive in Dot-ELISA. Eighty-eight (67.2%) of the remaining 131 sera were negative by both Dot-ELISA and CFT using AGE of both Campylobacter strains whereas 43 sera (32.8%) gave different reaction patterns in Dot-ELISA and CFT with the extracts of both Campylobacter strains. Twelve sera were positive by both tests using AGE of C. fetus ssp. fetus but CFT failed to detect antibodies in nine of these sera when AGE of C. jejuni was used. Twelve sera were positive by both tests only when AGE of C. fetus ssp. fetus was used. Eleven sera were positive only, by CFT. Seven of these reacted only with the AGE of C. fetus ssp. fetus and four sera were positive by using AGE of both Campylobacter strains. The remaining eight sera were found to be positive only by dot-immunobinding assay either with the AGE of both Campylobacter strains or with the AGE of one of the Campylobacter strains. It is concluded that Dot-ELISA using AGE from C. fetus ssp. fetus could be employed for the detection of Campylobacter antibodies in sheep sera and the additional use of AGE from C. jejuni as antigen appeared not to be profitable for this purpose.
Prevalence and Characteristics of Campylobacter Species Isolated From Gallbladder of Slaughtered Sheep in Van, (Eastern) Turkey
(veterinarni A Farmaceuticka Univerzita Brno, 2006) Ekin, IH; Gürtürk, K; Arslan, A; Boynukara, B
To determine the prevalence of campylobacter species in gallbladder of sheep in Van, ( Eastern) Turkey, a total of 220 gallbladder samples from healthy slaughtered sheep were examined bacteriologically in October 2000 and 2002. Of the 110 samples examined each year, 27 ( 24.6%) and 24 ( 21.8%) campylobacter strains were isolated, respectively. Of the 27 campylobacter strains isolated in the year 2000, 14 ( 51.9%) were identified as C. jejuni, 7 ( 25.9%) C. fetus, 3 ( 11.1%) C. coli and 3 ( 11.1%) C. lari. Similar results were obtained in the study performed in 2002, but C. lari could not be isolated. Growth and biochemical characteristics of all identified Campylobacter species with some exceptions were typical of each species. Six of 13 examined C. fetus strains grew well at both 25 degrees C and 42 degrees C in thioglycollate medium and on blood agar. C. jejuni strains differed from C. coli only by Na-hippurate hydrolysis test. Results of the present study revealed that C. jejuni is the most common campylobacter species isolated from gallbladders of sheep. The thermophilic campylobacters in significant proportions may cause contamination of carcass during slaughter and transmission of the food-borne pathogens to humans.