Browsing by Author "Genc, Aysun Inan"

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Role of the Probiotic Supplementation on Intestinal Inflammation and Structural Integrity in Wistar Rats Subjected To a Cafeteria Diet During Development
(Univ Agriculture, Fac veterinary Science, 2025) Onlu, Harun; Teker, Hikmet Taner; Keskin, Seda; Genc, Aysun Inan; Allahverdi, Huseyin; Elarslan, Aylin; Ceylani, Taha
Probiotics have gained significant interest in medical and veterinary sciences due to their potential to improve gastrointestinal health. This study investigates the protective role of probiotics on intestinal health in male Wistar rats exposed to a cafeteria diet during development. The experimental groups were divided into four: control, probiotics, cafeteria diet, and cafeteria diet with probiotics. Probiotics groups were administered daily at 1 x 108 CFU throughout the experiment. Ileum and colon tissues were analyzed via Fourier-transform infrared spectroscopy, histopathological analysis, and immunohistochemical staining. The cafeteria diet group showed altered lipid profiles, increased protein carbonylation (a marker of oxidative stress), and increased mast cell density, indicating increased intestinal inflammation. Probiotic supplementation significantly reduced inflammation by reducing TNF-alpha (P <= 0.0001) and IL-1(3 (P <= 0.0001). These results suggest that probiotic supplementation during an unhealthy diet can mitigate adverse effects by reducing oxidative stress and inflammation. Thus, probiotics could offer therapeutic potential in mitigating cafeteria diet-induced intestinal changes, serving as a promising dietary intervention during development to manage metabolic disorders in both humans and animals.
Therapeutic Potential of Young Plasma in Reversing Age-Related Liver Inflammation Via Modulation of Nlrp3 Inflammasome and Necroptosis
(Springer, 2025) Baba, Burcu; Ceylani, Taha; Teker, Hikmet Taner; Keskin, Seda; Genc, Aysun Inan; Gurbanov, Rafig; Acikgoz, Eda
The phenomenon of inflammaging, characterized by an increase in low-grade chronic inflammation, is closely associated with diseases related to liver dysfunction. This study investigated daily plasma exchange between 5-week-old and 24-month-old Sprague Dawley rats for 30 days, focusing on protein secondary structures, NLRP3 inflammasome, and necroptosis. Conformation changes in protein secondary structures were identified by infrared spectroscopy-based pattern recognition analysis. Liver biopsies with histochemical and immunohistochemical staining were used to assess molecules associated with inflammation, necroptosis and NLRP3 inflammasome complex. Expression levels of NLRP3 components were determined by qPCR. Enhanced random coils, 310 helices, beta-turns, and loop structures were identified in old rats and young rats with old plasma. Young rats and old rats with young plasma displayed higher alpha-helices and beta-sheet structures. Young rats with old plasma showed increased NLRP3, ASC, caspase-1, IL-1 beta, and IL-18 mRNA levels, indicating an inflammatory response. Whereas old rats with young plasma exhibited lower inflammation levels. Histological evaluations revealed that young rats receiving aged plasma showed significantly increased levels of NLRP3, ASC, caspase-1, IL-1 beta, TNF-alpha, VEGFR2, RIPK1, and MLKL immunoreactivity, whereas decreased immunoreactivity in aged rats receiving young plasma. These findings suggest that young plasma reduces NLRP3 inflammasome activation and necroptosis in aged rats.