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Browsing by Author "Goktepe, Ozge"

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    Article
    Caffeine Increased Antitumor Effects of Paclitaxel (Ptx) in Mcf-7 and Mda-Mb Breast Cancer Cells
    (Marmara Univ, 2023) Aydin, Funda; Onder, Gozde Ozge; Goktepe, Ozge; Bitgen, Nazmiye
    Although progress has been made in the diagnosis and treatment of breast cancer, which is one of the most important causes of women's health, it is still one of the leading causes of death in women. One of the most prominent causes for this is illness resistance to the medications employed in treatment. For this reason, the trend towards combination therapy research has increased in addition to conventional therapy. In this study, we aimed to investigate the effect of co-administration of caffeine (CAF) and paclitaxel (PTX), which is frequently used in breast cancer, on MDA-MB-231 and MCF-7 cells. For this purpose, 4 groups were determined as control, CAF, PTX and CAF+ PTX. MTT assay was used to assess cell viability and the appropriate dose for CAF was determined. The apoptotic effect of the drug combination on cell lines was evaluated with the TUNEL method, and it was determined at what stage it paused cell division by cell cycle analysis. According to the study's findings, the results indicated that CAF induced apoptosis in breast cancer cells and the best effect was in the group administered with PTX. Furthermore, it was discovered that CAF and PTX in the MCF-7 cell lines, both together and separately, blocked cell division in the S phase in MCF-7 cell lines. These results are promising for future studies that will prove the usefulness of CAF as an adjuvant in the treatment of breast cancer.
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    Article
    Therapeutic Potential of Hesperidin: Apoptosis Induction in Breast Cancer Cell Lines
    (Pergamon-elsevier Science Ltd, 2023) Onder, Gozde Ozge; Goktepe, Ozge; Baran, Munevver; Bitgen, Nazmiye; Aydin, Funda; Yaya, Arzu
    Hesperidin is a flavonoid commonly found in citrus fruits. Studies have shown that hesperidin has anti-inflammatory, analgesic, and antimicrobial properties, as well as its effectiveness in carcinogenesis. In this paper, we aim to investigate the molecular mechanisms of hesperidin-induced apoptosis in MCF-7 and MDA-MB-231 cancer cells.The inhibitory effect of hesperidin on cellular proliferation was evaluated with the MTT assay. Cell cycle analysis of hesperidin-treated cells was then performed, as well as immunocytochemical analysis of the effect on the apoptosis pathway (TUNEL, Bax, and Bcl-2 expression).Moreover, hesperidin induced cellular apoptosis in MCF-7 breast cancer cells by inhibiting Bcl-2 and enhancing Bax expression at protein levels. On the other hand, hesperidin caused apoptosis in the MDA-MB-231 breast cancer cell line, but it did not activate the Bax/Bcl-2 pathway. Hesperidin also induced cell cycle arrest at the S phase in the MCF-7 and MDA-MB-231 cell lines.These findings showed that hesperidin is a potential therapeutic candidate for preventing the progression of breast cancer. In addition, hesperidin could significantly stimulate the death mechanisms in ER/PR (+) MCF-7 cells by changing the expression balance of Bax and Bcl-2 proteins, but lead ER/PR (-) MDA-MB-231 breast cancer cells to apoptosis in a different way.