Browsing by Author "Goren, Ahmet C."

Now showing 1 - 5 of 5

Analysis of Phenolic Compounds by Lc-Hrms and Determination of Antioxidant and Enzyme Inhibitory Properties of Verbascum Speciousum Schrad
(Acg Publications, 2023) Kiziltas, Hatice; Bingol, Zeynebe; Goren, Ahmet C.; Alwasel, Saleh H.; Gulcin, Ilhami
Studies have shown an inverse correlation between age-related illnesses like coronary heart disease and cancer and fruit and vegetable intake. Given the probable health benefits of natural antioxidants from plants, research on them has increased. Verbascum L is a large genus of Scrophulariaceae family and 323 species distributed worldwide. Since Verbascum L. species are plants that are grown in many regions of Turkiye and are used in folk medicine, it is important to evaluate the biological activity of these species. In this study antioxidant properties of Verbascum speciosum Schrad. were investigated. The antioxidant capacities of water and ethanol-based extracts obtained from air parts were evaluated with Fe3+ reducing, CUPRAC, FRAP, DPPH center dot, and ABTS(center dot+) scavenging antioxidant methods. Extracts were also examined to determine their AChE, alpha-glycosidase and alpha-amylase enzyme inhibitions. This investigation could be a basis for further phytochemical investigations of Verbascum speciosum Schrad.
Anticholinergic, Antidiabetic and Antioxidant Activities of Ferula Orientalis L. Determination of Its Polyphenol Contents by Lc-Hrms
(Acg Publications, 2021) Kiziltas, Hatice; Goren, Ahmet C.; Bingol, Zeynebe; Alwasel, Saleh H.; Gulcin, Ilhami
To evaluate the antioxidant activity of evaporated ethanolic extract of Ferula orientalis L. (EEFO) and lyophilized water extract of Ferula orientalis L. (WEFO) several in vitro antioxidant methods such as ABTS(center dot+) scavenging activity, DPPH. scavenging activity, Fe(3+)reduction method, cupric ions (Cu2+) reduction capacity, and metal ion (Fe2+)-binding activities using ferrozine reagent were separately performed. Also, BHT, alpha-tocopherol and ascorbic acid were used as the standard antioxidant molecules. Moreover, some phenolic compounds that are responsible for antioxidant abilities of EEFO and WEFO were determined by LC-HRMS. EEFO and WEFO demonstrated effective antioxidant abilities when compared with the standards. EEFO demonstrated IC50 values of 1.946 mu g/mL against acetylcholinesterase (AChE), 0.815 mu g/mL against alpha-glycosidase, and 0.675 mu g/mL against alpha-amylase.
Antidiabetic, Anticholinergic and Antioxidant Activities of Aerial Parts of Shaggy Bindweed (Convulvulus Betonicifolia Miller Subsp.) - Profiling of Phenolic Compounds by Lc-Hrms
(Elsevier Sci Ltd, 2021) Bingol, Zeynebe; Kiziltas, Hatice; Goren, Ahmet C.; Kose, Leyla Polat; Topal, Meryem; Durmaz, Lokman; Gulcin, Ilhami
In order to evaluate the antioxidant activity of evaporated ethanolic extract (EESB) and lyophilized water extract (WESB) of Shaggy bindweed (Convulvulus betonicifolia Mill. Subs), some putative antioxidant methods such as DPPH. scavenging activity, ABTS(center dot)(-) scavenging effect, ferric ions (Fe3+) reduction method, cupric ions (Cu2+) reducing capacity, and ferrous ions (Fe2+) binding activities were separately performed. Also, ascorbic acid, alpha-tocopherol and BHT were used as the standard compounds. Additionally, some phenolic compounds that responsible for antioxidant abilities of EESB and WESB were screened by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the same concentration, EESB and WESB demonstrated effective antioxidant abilities when compared to standards. In addition, EESB demonstrated IC50 values of 1.946 mu g/mL against acetylcholinesterase (AChE), 0.815 mu g/mL against alpha-glycosidase and 0.675 mu g/mL against alpha-amylase enzymes.
Essential Oil Compositions and Antimicrobial Activity of Salvia Species
(Taylor & Francis Ltd, 2007) Altun, Mehmet; Unal, Murat; Kocagoz, Tanil; Goren, Ahmet C.
The essential oil compositions of aerial parts of Salvia macroclamys, S. verticillata ssp. amasiaca, S. virgata, S. multicaulis, S. firigida, S. microstegia and S. kronenburgii were analyzed by gas chromatography-mass spectrometry (GC-MS). Their main constituents were found to be alpha-pinene, beta-pinene, 1,8-cineole, thymol, and caryophyllene oxide. Essential oil of S. multicaulis, S. kronenburgii, S. verticillata and the pure compounds alpha-pinene, beta-pinene, 1,8-cineole, linalool oxide and caryophyllene oxide were tested against E. coli, S aureus, K. pneumonia, and the yeast C.albicans. Key words: Salvia, essential. oil, antimicrobial activity, GC-MS, alpha-pinene, beta-pinene,
Lc-Hrms Profiling of Phytochemicals, Antidiabetic, Anticholinergic and Antioxidant Activities of Evaporated Ethanol Extract of Astragalus Brachycalyx Fischer
(Acg Publications, 2021) Kiziltas, Hatice; Bingol, Zeynebe; Goren, Ahmet C.; Pinar, Suleyman Mesut; Alwasel, Saleh H.; Gulcin, Ilhami
Astragalus is a perennial plant that has existed for about 2500-3000 years and consists of more than 250 taxonomic parts. Twenty species of Astragalus are endemic to Turkey, as well as the richest genus with 425 taxa. The roots of Asfragalus species are used in folk medicine as hepatoprotective, antioxidant, antibacterial, antihypertensive, antidiabetic and diuretic. Also, it is used to treat diabetes mellitus, leukemia, nephritis and uterine cancer. It is known that in Anatolia, Astragalus roots are traditionally used against leukemia and wound healing. For the purpose, the measuring of antioxidant activity of evaporated ethanol extract of Asfragalus brachycalyx FISCHER (EEAB), some bioanalytic methods including DPPH center dot and ABTS(center dot+) scavenging effects, ferric ions (Fe3+) and cupric ions (Cu2+) reducing abilities, and metal (Fe') chelating activity were realized. alpha-Tocopherol, ascorbic acid, and BHT were used as the standard antioxidants. On the other hand, some phenolic compounds, which responsible for antioxidant activities of EEAB was determined by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the similar concentration, EEAB exhibited efficient antioxidant effects when compared to standard compounds. Additionally, EEAB showed IC50 values of 1.985 mu g/mL toward acetylcholinesterase (AChE), 0.620 mu g/mL on alpha-glycosidase and 0.306 mu g/mL against alpha-amylase enzymes.