Browsing by Author "Goz, Yasar"

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Chewing Lice (Phthiraptera: Amblycera, Ischnocera) on Several Species of Wild Birds Around the Lake Van Basin, Van, Eastern Turkey
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Goz, Yasar; Dik, Bilal; Kilinc, Ozlem Orunc; Yilmaz, Ali Bilgin; Aslan, Logman
This study was performed to detect chewing lice on the wild birds in Eastern Turkey, between April 2013-September 2014. 108 injured birds brought to Wild Animal Protection Center of Yuzuncu Yil University were examined for louse. The feathers of each bird specimens were inspected for louse, macroscopically. Collected lice samples on the birds were preserved in 70% ethyl alcohol and mounted on slides in Canada balsam after transparented in 10% KOH. Fifteen (14.95%) out of the 108 were found to be infested with at least one chewing louse species. Nineteen lice species in 15 genera were found on the infested birds. Goniocotes megalocephalus (Uchida, 1916) on the Helmeted Guineafowl (Numida meleagris); Actornithophilus piceus lari (Packard, 1870) on the Armenian Gull (Larus armenicus); Kurodaia fulvofasciata (Piaget, 1880) on the Long-legged Buzzard (Buteo rufinus); Laemobothrion sp. on the Golden Eagle (Aquila chrysaetos); Trinoton anserinum (Fabricius [J.C.], 1805) and Holomenopon sp. on the Greylag Goose (Anser anser) were recorded for the first time from Turkey in this study.
Detection of Putative Virulence Genes in Aeromonas Isolates From Humans and Animals
(J infection developing Countries, 2014) Korkoca, Hanifi; Alan, Yusuf; Bozari, Sedat; Berktas, Mustafa; Goz, Yasar
Introduction: Aeromonas are food-and water-borne bacteria that are considered to be zoonotic human pathogens. This study aimed to investigate the presence of genes associated with virulence in human and animal Aeromonas isolates and the potential role of animal isolates with regards to human Aeromonas infections. Methodology: The presence of aerA, hlyA, alt, ast, laf, ascF-G, stx1 and stx2 putative virulence genes in 40 human and animal Aeromonas isolates (16 human and 24 animal isolates) were examined by polymerase chain reaction (PCR). DNA fragments of expected sizes were purified and sequenced. BLAST in the NCBI was used to verify any amplified products. Results: PCR screening showed that hlyA, alt, and laf genes were determined at ratios of 6.25%, 50%, and 6.25%, respectively, in human isolates. The ratios of hlyA, alt, ascF-G, laf, stx2, and stx1 genes in animal isolates were 58.3%, 20.83%, 33.3%, 20.83%, 8.33%, and 4.17%, respectively. Neither aerA nor ast genes were detected in any isolates. Any one of eight putative virulence genes was not detected in seven human and eight animal isolates in the study. Conclusions: The current study is the first to investigate the presence of the virulence gene in gull Aeromonas isolates. The manifestation of the presence of the virulence gene and gene combinations was considerable, especially in fish and gull isolates when compared with clinical human isolates. The current study demonstrates the potential importance of fish and gulls in terms of human Aeromonas infections.
Determination of Cryptosporidium Spp. in Van Cats by Nested Pcr
(Polish Soc veterinary Sciences Editorial office, 2018) Kilinc, Ozlem Orunc; Yilmaz, Ali Bilgin; Goz, Yasar; Ozkan, Cumali; Denizhan, Vural
The present study was conducted to investigate the presence of Cryptosporidium spp. agents in cats from the Turkish Van Cat Shelter at YuzuncuYil University by a modified Ziehl-Neelsen staining method and nested PCR. Individual stool samples were obtained from 30 adult females, 30 adult males and 40 kittens - a total of 100 Van cats were analyzed in the study. A simplified formol-ether concentration method was applied to all samples. The samples were then examined microscopically by a modified Ziehl-Neelsen staining method. As a result of the staining, Cryptosporidium spp. oocysts were identified in stool samples of 3 kittens in the microscopic examination. After that, PCR and nested-PCR were conducted with suitable primers. Nested PCR identified 5 kittens (5%) as positive. As a result, it was concluded that nested PCR was a superior diagnostic method for Cryptosporidium diagnosis compared with staining methods and that infected cats could be a health hazard for other cats and individuals, since Cryptosporidium spp. agents infect via the faecal-oral route. Therefore, we believe it is necessary to raise the awareness of people in contact with cats.
Determination of Serum Cardiac Biomarkers and Plasma D-Dimer Levels in Anemic Sheep With Babesiosis
(Tubitak Scientific & Technological Research Council Turkey, 2015) Orunc Kilinc, Ozlem; Goz, Yasar; Yuksek, Nazmi; Basbugan, Yildiray; Yilmaz, Ali Bilgin; Atas, Ahmet Duran
In this study, the cardiac effects of anemia and venous thromboembolism (VTE), reportedly caused by the hemolyzed erythrocytes occurring in sheep with babesiosis, were investigated using cardiac markers and D-dimer (DD). The study included 34 sheep: 24 infected Akkaraman sheep (1-3 years old, diagnosed with babesiosis based on clinical and laboratory findings) and a control group of 10 noninfected healthy sheep of the same breed and age. Hematocrit (Hct) levels were measured in blood obtained from both groups. Cardiac troponin I (cTnI), creatine kinase-MB (CK-MB), and aspartate aminotransferase (AST) levels were measured in serum samples. In addition, the levels of DD were also measured in plasma samples. Sheep with babesiosis were subsequently divided into 3 subgroups according to their Hct levels, which ranged from 13.2% to 16.3% in the first group (n = 8), 20.1% to 25.6% in the second group (n = 8), and 27.4% to 30.3% in the third group (n = 8). Evaluations of the measurements of cTnI, CK-MB, and AST levels indicated statistically significant differences between infected and healthy sheep. Statistically significant differences were not found for DD levels between the 2 groups.
First Report on Heavy Uncinaria (Dochmoides) Sp (Nematoda: Ancylostomatidae) Infection in Brown Bear (Ursus Arctos) Cub, in Van Province, Eastern Anatolian Region of Turkey
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Orunc Kilinc, Ozlem; Goz, Yasar; Yilmaz, Ali Bilgin; Aslan, Logman
On April 2014, 6 months old male Brown bear cub that found in the Saray district of Van province was brought into Directorship of Wild Animal Protection of University of Yuzuncu Yil. Despite all the intervation, bear cub, very poor condition in clinically, dead after two days. In postmortem patological and parasitological examination were detecded severe hemorrhagic enteritis and many hookworms with white colour and approximately 1 cm diameter in surface of the small intestine. In stereo microscopic examinations, it was concluded that this worms are Uncinaria spp. This case report represents the first time Uncinaria spp. have been reported in Brown bear in Turkey.
Investigation of Inhibition Effect of Butanol and Water Extracts of Matricaria Chamomilla L. on Angiotensin-Converting Enzyme Purified From Human Plasma
(Wiley, 2022) Bas, Zehra; Turkoglu, Vedat; Goz, Yasar
Angiotensin-converting enzyme (ACE) liable for the regulation of blood pressure was purified from human plasma by affinity chromatography. Impact of water and butanol extracts of Matricaria chamomilla L. on purity ACE was examined. ACE was purified using the affinity chromatography method. The enzyme activity was evaluated at 345 nm by a . Extracts of M. chamomilla plant with butanol and water were prepared. Lisinopril was utilized as a specific inhibitor. ACE was purified 3,659-fold from human plasma and the specific activity was 1,350 EU/mg protein. The molecular weight and purity of ACE were found by SDS-PAGE and two bands of 60 and 70 kDa on the gel were detected. Water and butanol extracts of M. chamomilla demonstrated inhibitor impact on ACE activity. IC50 constants for water and butanol extracts of M. chamomilla were computed to be 1.292 and 0.353 mg/mL, respectively. The type of inhibition for whole inhibitors was identified as noncompetitive. IC50 and K-i constants for lisinopril were calculated to be 0.781 and 0.662 nM, respectively. These results indicate that butanol and water extracts of M. chamomilla may have an ACE inhibitor potential.
Molecular Detection of Neospora Caninum From Naturally Infected Dogs in Van Province, East Turkey
(Agricultural Research Communication Centre, 2020) Yilmaz, Ali Bilgin; Goz, Yasar; Kilinc, Ozlem Orunc; Denizhan, Vural
Neospora caninum is a coccidian parasite causing abortion in cattle and neurological problems in horses. Dogs are definitive hosts of N. caninum. Polymerase chain reaction is the most specific method used for the detection of N. caninum oocytes. In the present study, a total of 100 fecal samples were collected from naturally infected dogs. Of the 100 samples analyzed, 11 of them were detected with Hammondia/Neospora-like oocytes. Genomic DNA was isolated using a commercially available DNA extraction kit. The Nc5 gene specific to N. caninum was amplified by PCR and two of the eleven samples with Hammondia/Neospora-like oocytes formed similar to 337 bp repeatable band. In conclusion, N. caninum, which has been shown to cause neurological disorders in dogs and to abortion in cattle, was detected in naturally infected dogs in Van Province in Polymerase chain reaction.
Parasites Detected in Neonatal and Young Calves With Diarrhoea
(Natl veterinary Research inst, 2006) Goz, Yasar; Altug, Nuri; Yuksek, Nazmi; Ozkan, Cumali
This study was performed between December 2000 May 2005 to detect parasitic diarrhoea agents in neonatal and young calves. In this period, faecal samples were obtained from 231 neonatal and young calves. Age of the calves ranged from I day to 8 months. Faecal samples were examined using native Lugol's solution, Fulleborn's salty water technique and modified Ziehl-Neelsen staining technique for Cryptosporidium. The parasitological examination revealed that 188 (81.3%) of the calves were infected with one or more parasitic agents.
Parasitic Protozoans (Eimeria, Giardia, and Cryptosporidium) in Lambs With Diarrhoea in the Van Province (Turkey)
(Natl veterinary Research inst, 2009) Ozdal, Nalan; Tanritanir, Pinar; Goz, Yasar; Deger, Serdar; Kozat, Suleyman
This study was conducted to investigate the prevalence of parasitic protozoan agents in lambs with diarrhoea in the Van province (Eastern Turkey). Faecal samples from 132 diarrhoeic lambs aged from one day to two months old were collected at 12 different herds from January 2005 to May 2005. The faecal samples were examined by using, native-Lugol, Fulleborn's salty water technique, and a Modified Ziehl-Neelsen staining technique for Cryptosporidium. The parasitological examination revealed that 98 (74.24%) of the lambs were infected with one or more of parasitic protozoan agents. Eimeria, Giardia, and Cryptosporidium were detected in 80 (60.60%), 64 (48.48%), and 18 (13.63%) of the lambs, respectively. According to statistical analysis, Eimeria sp. oocysts were significantly higher (P < 0.01) in lambs aged between 3 1 and 60 d (76.81%, 53 of 69) than in those of 16-30 d of age (50%, 21 of 42) and 1-15 d of age (28.57%, 6 of 2 1). There were significant differences in Giardia prevalance when lambs of 1-15 d of age were compared with those 16-30 d of age (P < 0.05). Statistical analysis revealed that Cryptosporidium sp. oocysts were significantly higher (P < 0.01) in lambs aged between I and 15 d (66.66%, 14 of 21) than in those of 16-30 d of age (4.76%, 2 of 42) and 31-60 d of age (2.89%, 2 of 69). Moreover, Giardia sp. and Eimeria sp. showed significant co-occurrence (34.84%). The results obtained confirmed the fact that protozoan infections are widespread in diarrhoeic lambs in this geographical region.
Prevalence and Molecular Characterization of Moniezia Species in Ruminants Based on Its1-5.8s Rrna From Van Province, Turkey
(Univ Agriculture, Fac veterinary Science, 2025) Yilmaz, Ali Bilgin; Azizoglu, Erkan; Adizel, Ozdemir; Goz, Yasar; Celik, Burcak Aslan; Celik, Ozgur Yasar; Uslu, Ugur
This study aimed to calculate the occurence of Moniezia species in cattle, sheep, and goats in Van province and to identify these species using morphological and molecular methods (ITS1-5.8S rRNA gene region analysis). Additionally, the study aimed to identify the genetic differences between Moniezia expansa and Moniezia benedeni. During the summer of 2022, intestinal contents were collected from 150 ruminants (50 cattle, 50 sheep, and 50 goats) slaughtered in slaughterhouses in Van province. The parasites were examined using Aceto-Carmine staining, and species identification was based on interproglottidal glands. Examination of the intestinal contents revealed that 2 out of 50 cattle (4%), 14 out of 50 sheep (28%), and 9 out of 50 goats (18%) were infected with Moniezia. Morphological and molecular analyses showed that the cattle samples were identified as Moniezia benedeni, goats samples as Moniezia expansa and those from sheep as 11 Moniezia expansa and 3 Moniezia benedeni. Following DNA extraction, the ITS1-5.8S rRNA gene region was amplified using PCR and subjected to sequence analysis. The relationship between species was examined by phylogenetic tree. This study confirms the prevalence of Moniezia spp. in Van/T & uuml;rkiye by using the ITS1-5.8S rRNA gene.
Prevalence of Cryptosporidiosis in Goats of Van Province Turkey
(indian veterinary Journal, 2006) Goz, Yasar; Ceylan, Ebubekir; Aydin, Abdulalim
Cryptosporidium is a minute coccidian protozoon belonging to phylum Apicomplexa. Twenty species of this parasite have been described from a variety of vertebrates including mammals, birds, reptiles and fish. Development of the Cryptosporidium occurs within the brush border. of the epithelial cells of the intestine. Sporulated oocysts, containing four sporozoits are passed in the faeces and are transmitted by ingestion (Markell, et al., 1992). The aim of this study was to determine the prevalence of Cryptosporidiosis in goats in province Van. This paper is the first report on the prevalence of Cryptosporidiosis in goats in Van province, eastern Turkey.
Serum Iron, Total Iron-Binding Capacity, Unbound Iron-Binding Capacity, Transferrin Saturation, Serum Copper, and Hematological Parameters in Pregnant Akkaraman Ewes Infected With Gastro-Intestinal Parasites
(Tubitak Scientific & Technological Research Council Turkey, 2006) Kozat, Suleyman; Yuksek, Nazmi; Goz, Yasar; Keles, Ihsan
The aim of this study was to investigate serum iron parameters, and copper and iron deficiency anemia in ewes infected with gastro- intestinal parasites. This study was conducted with 60 pregnant Akkaraman ewes. In the statistical analyses, values obtained from both parasite infected and uninfected ewes were compared. Significant decreases were observed in PCV (P < 0.05), Hb (P < 0.05), RBC (P < 0.05), total protein (P < 0.05), albumin (P < 0.05), globulin (P < 0.05), and MCHC (P < 0.01), and a significant increase was seen in the WBC value (P < 0.05) in the parasite infected group. Furthermore, the parasite infected group had lower serum Fe (P < 0.001), TS % (P < 0.001), and serum Cu (P < 0.01) levels, and had higher UIBC values (P < 0.05) compared to the values obtain from the uninfected group. Therefore, in addition to antiparasite drug treatment in parasite infected pregnant ewes, iron preparations should also be administered in order to treat iron deficiency.
Ticks and Fleas Infestation on East Hedgehogs (Erinaceus Concolor) in Van Province, Eastern Region of Turkey
(Iranian Scientific Society Medical Entomology, 2016) Goz, Yasar; Yilmaz, Ali Bilgin; Aydin, Abdulalim; Dicle, Yalcin
Background: Ixodid ticks (Acari: Ixodidae) and fleas (Siphonaptera) are the major vectors of pathogens threatening animals and human healths. The aim of our study was to detect the infestation rates of East Hedgehogs (Erinaceus concolor) with ticks and fleas in Van Province, eastern region of Turkey. Methods: We examined fleas and ticks infestation patterns in 21 hedgehogs, collected from three suburbs with the greater of number gardens. In order to estimate flea and tick infestation of hedgehogs, we immobilized the ectoparasites by treatment the body with a insecticide trichlorphon (Neguvon (R)-Bayer). Results: On the hedgehogs, 60 ixodid ticks and 125 fleas were detected. All of the ixodid ticks were Rhipicephalus turanicus and all of the fleas were Archaeopsylla erinacei. Infestation rate for ticks and fleas was detected 66.66 % and 100 %, respectively. Conclusion: We detected ticks (R. turanicus) and fleas (A. erinacei) in hedgehogs at fairly high rates. Since many ticks and fleas species may harbor on hedgehogs and transmit some tick-borne and flea-borne patogens, this results are the important in terms of veterinary and public health.