Browsing by Author "Gul, Mustafa"

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Detection of the Frequency of Per-1 Type Extended-Spectrum Β-Lactamase Acinetobacter Baumannii Clinical Isolates in Turkey: a Multicenter Study
(Tubitak Scientific & Technological Research Council Turkey, 2014) Asik, Gulsah; Ozdemir, Mehmet; Kurtoglu, Muhammet Guzel; Yagci, Server; Oksuz, Lutfiye; Gul, Mustafa; Ciftci, Ihsan Hakki
Background/aim: beta-Lactamases are an important resistance mechanism in Acinetobacter baumannii. Pseudomonas extended-resistance (PER-1) type beta-lactamase-producing strains have been reported from various geographic locations; however, PER-1 type beta-lactamases from Turkish hospitals have not been investigated extensively. The aim of this study was to determine the prevalence of PER-1 type beta-lactamases in A. baumannii isolates in various regions of Turkey. Materials and methods: A total of 763 clinical A. baumannii isolates were collected from 9 university hospitals and 2 state hospitals between 2008 and 2011. Molecular amplification of the OXA-51 gene from the A. baumannii genome was performed in order to verify identification of the species. Real-time polymerase chain reaction was used to detect bla(PER-1) genes. Results: PER-1 was detected in 24.6% of the isolates. The annual frequencies of the PER-1 enzyme were detected as 52.2%, 35.9%, and 8.3% in 2008, 2009, and 2010, respectively. PER-1 prevalence decreased gradually over time. The differences observed in PER-1 prevalence among the regions of Turkey were statistically significant (chi-square test; P < 0.001). Conclusion: These data demonstrate that the frequency of detection of PER-1 type beta-lactamases in A. baumannii species has decreased in Turkey. However, the increased carbapenem resistance, together with multidrug resistance, has created a worrisome situation regarding this pathogen.
Oxidative Stress Before and After Surgery in Benign Prostatic Hyperplasia Patients
(Wiley, 2019) Ercan, Mehlika; Alp, Hamit Hakan; Kocaturk, Huseyin; Bakan, Nuri; Gul, Mustafa
The aim was to assess oxidative stress in benign prostatic hyperplasia patients and also to evaluate the effect of operation in late (60 days) post-operative period. This study was conducted with 16 patients with benign prostatic hyperplasia and 16 healthy subjects. Serum malondialdehyde, blood 8-hydroxy-2 '-deoxyguanosine/deoxyguanosine, erythrocyte superoxide dismutase, serum total coenzyme Q(10) and coenzyme Q(10) levels were measured. Independent samples t test was used to analyse the differences between control group and patients, while paired t test was used to analyse the differences between pre-operative and post-operative periods. Malondialdehyde and total coenzyme Q(10) levels were lower in patients, while 8-hydroxy-2 '-deoxyguanosine/deoxyguanosine level was increased. However, superoxide dismutase activity and coenzyme Q(10) levels did not differ. After 60 days of operation, 8-hydroxy-2 '-deoxyguanosine/deoxyguanosine and superoxide dismutase activity decreased, while total coenzyme Q(10) level increased. However, malondialdehyde and coenzyme Q(10) levels were not affected. The international prostate symptom scores of the patients were also decreased after the operation. The results suggest that blood 8-hydroxy-2 '-deoxyguanosine/deoxyguanosine level may reflect the oxidative stress better than the malondialdehyde level, and surgical operation attenuates the oxidative stress in late post-operative period in benign prostatic hyperplasia patients.