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Browsing by Author "Gulcin, Lhami"

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    Article
    Comprehensive Metabolic Profiling of Acantholimon Caryophyllaceum Using Lc-Hrms and Evaluation of Antioxidant Activities, Enzyme Inhibition Properties and Molecular Docking Studies
    (Elsevier, 2022) Kiziltas, Hatice; Bingol, Zeynebe; Goren, Ahmet Ceyhan; Pinare, Sueuroleyman Mesut; Ortaakarsu, Ahmet Bugra; Alwasel, Saleh H.; Gulcin, Lhami
    The Acantholimon taxon consists of thorny, perennial and subshrub flowering plants. Extraction of various Acantholimon species are traditionally applied to treat liver disease and diabetes and roots are boiled used to treat wounds and skin irritation. Here, we quantified, the radical scavenging activities of ethanol (EEAC) and water (WEAC) extracts of Acantholimon caryophyllaceum Boiss (A. caryophyllaceum) against 1,1-diphenyl-2-picryl-hydrazyl (DPPHGreek ano teleia) and 2,2-azino-bis3-ethylbenzthiazoline-6-sulfonic acid (ABTS*+) as well as their abil-ity to reduce Fe3+, Fe3+-TPTZ, and Cu2+. The antioxidant activities of both extracts were similar to those of standards in all assays. For instance, IC50 of EEAC and WEAC for scavenging DPPH were 19.8 and 69.3 mg/mL, respectively. We also assessed the inhibitory effects of EEAC and WEAC against acetylcholinesterase (AChE), a-amylase and a-glycosidase, which are associated with Alzheimer's disease and diabetes. The ethanol extract inhibited these enzymes with IC50 of 1.137, 0.172 and 0.511 mg/mL, respectively. The results of liquid chromatography-high resolution mass spectrometry (LC-HRMS) revealed that (-)-epigallocatechin was the main phenolic compound in both EEAC and WEAC. Molecular docking analysis revealed binding interactions between the main phenolic compounds (-)-epigallocatechin gallate, (-)-epigallocatechin, fumaric acid, hyperoside, and myricetin with AChE, a-amylase and a-glycosidase enzymes.(c) 2022 Published by Elsevier B.V. on behalf of SAAB.
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    Article
    Potential Antioxidant, Anticholinergic, Antidiabetic and Antiglaucoma Activities and Molecular Docking of Spiraeoside as a Secondary Metabolite of Onion (Allium Cepa)
    (Elsevier, 2023) Durmaz, Lokman; Kiziltas, Hatice; Karagecili, Hasan; Alwasel, Saleh; Gulcin, Lhami
    Onion contains many dietary and bioactive components including phenolics and flavonoids. Spiraeoside (quercetin-4-O-b-D-glucoside) is one of the most putative flavonoids in onion. Several antioxidant techniques were used in this investigation to assess the antioxidant capabilities of spiraeoside, including 1,1diphenyl-2-picrylhydrazyl radical (DPPHGreek ano teleia) scavenging, N,N-dimethyl-p-phenylenediamine radical (DMPD'') scavenging, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical (ABTS'') scavenging activities, cupric ions (Cu2') reducing and potassium ferric cyanide reduction abilities. In contrast, the water-soluble a-tocopherol analogue trolox and the conventional antioxidants butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and a-tocopherol were utilized as the standards for evaluation. Spiraeoside scavenged the DPPH radicals an IC50 of 28.51 mu g/mL (r2: 0.9705) meanwhile BHA, BHT, trolox, and a-tocopherol displayed IC50 of 10.10 mu g/mL (r2: 0.9015), 25.95 mu g/mL (r2: 0.9221), 7.059 mu g/mL (r2: 0.9614) and 11.31 mu g/mL (r2: 0.9642), accordingly. The results exhibited that spiraeoside had effects similar to BHT, but less potent than a-tocopherol, trolox and BHA. Also, inhibitory effects of spiraeoside were evaluated toward some metabolic enzymes including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase II (CA II) and a-glycosidase, which are related to a number of illnesses, such as Alzheimer's disease (AD), diabetes mellitus and glaucoma disorder. Spiraeoside exhibited IC50 values of 4.44 nM (r2: 0.9610), 7.88 nM (r2: 0.9784), 19.42 nM (r2: 0.9673) and 29.17 mM (r2: 0.9209), respectively against these enzymes. Enzyme inhibition abilities were compared to clinical used inhibitors including acetazolamide (for CA II), tacrine (for AChE and BChE) and acarbose (for a-glycosidase). Spiraeoside demonstrated effective antioxidant, anticholinergic, antidiabetic and antiglaucoma activities. With these properties, it has shown that Spiraeoside has the potential to be a medicine for some metabolic diseases. (c) 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).