Browsing by Author "Gultekin, Mehmet"

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Comparative Clinical Efficiency of Resveratrol, Allopurinol, and Resveratrol Plus Allopurinol in Canine Visceral Leishmaniasis
(Amer veterinary Medical Assoc, 2025) Erdogan, Songul; Erdogan, Hasan; Ural, Kerem; Pasa, Serdar; Gultekin, Mehmet; Balikci, Canberk; Gunal, Ismail
Objective To assess the clinical and immunological effectiveness of resveratrol alone or combined with allopurinol for treating canine visceral leishmaniasis (CVL). Methods This study included 50 dogs, 30 diagnosed with CVL and 20 healthy controls. Dogs were classified based on their treatment regime (receiving resveratrol [200 mg/dog/d] and/or allopurinol [10 mg/kg/d]). Clinical scores, antileishmanial antibody titers, and routine blood parameters were evaluated on days 0, 15, and 30 after treatment. Results Dogs treated with the combination therapy showed the most substantial improvements, with a 75.77% reduction in clinical scores and an 81.25% reduction in antibody titers. Dogs receiving allopurinol exhibited a 56.25% reduction in clinical scores, while those treated with resveratrol showed a 42.82% reduction. Antibody titers were less signifi-cantly reduced in the resveratrol group (67.5%) compared to the allopurinol group (45%). Conclusions Combining resveratrol with allopurinol enhances the therapeutic efficacy for CVL, leading to significant clinical im-provements and reductions in antibody titers. Resveratrol alone also demonstrates promising anti-leishmanial effects. Clinical Relevance Resveratrol, either alone or in combination with allopurinol, offers a feasible and effective alternative for improving clinical outcomes in CVL. Its incorporation into standard protocols may enhance treatment strategies in veterinary medicine.
Prevalance and Molecular Characterization of Giardia Duodenalis in Livestock in Van, Turkey
(Health & Environment Assoc, 2019) Ayan, Adnan; Ural, Deniz Alic; Erdogan, Hasan; Kilinc, Ozlem Orunc; Gultekin, Mehmet; Ural, Kerem
Giardia duodenalis (Giardia lambia, Giardia intestinalis), a flagellated parasite, is a gastrointestinal pathogen that affects public health worldwide. There are limited studies on the epidemiology and molecular characterization of G. duodenalis in farm animals in Turkey. Molecular characterization is requited to determine the zoonotic potential of Giardia infection. In the present study, 71 calf 50 lamb, and 66 goat fecal stool samples were obtained from the animal rectum using a disposable latex glove and the samples were transferred to the laboratory. Then, the presence of cysts was examined with a microscope using of saturated zinc sulfate flotation method (ZnSO4; 33%). Afterwards, the samples were scanned with ELISA-based rapid diagnostic test kits for diagnosis. Then, DNA was extracted with QIAamp DNA Stool Mini Kit (Qiagen, Germany). Nested PCR was then conducted with the corresponding primers. DNA sequence analysis was conducted on beta-giardin gene region of each PCR positive samples. Then, sequence analyses were blasted and compared with the access numbers in the gene bank (M36728 for sub-genotype A1, AY072723 for sub-genotype A2, for AY072724 sub-genotype A3, AY072725 for subgenotype B1, AY072726 for sub-genotype B2, AY072727 for sub-genotype B3, AY072728 for sub-genotype B4). In the microscopic examination, Giardia cysts were identified in 41 calves, 18 lambs, and 23 goats. Positivity was determined with rapid test kits in 38 calves, 16 lambs, and 20 goats. In nested PCR results, specific bands (511 bp) were obtained in 46 calves, 21 lambs and 24 goats. Sequencing findings demonstrated Assemblage A and subgenotype A3 presence in all animal samples. The importance of treatment and the need to take protective measures due to its zoonotic importance were emphasized.