Browsing by Author "Gulyuz, Fetih"

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Effect of Chrysin on Methotrexate-Induced Testicular Damage in Rats
(Wiley, 2019) Belhan, Saadet; Comakli, Selim; Kucukler, Sefa; Gulyuz, Fetih; Yildirim, Serkan; Yener, Zabit
This study was conducted on 28 male Wistar albino rats to determine the effects of chrysin on methotrexate-induced damage to testicular tissue. Rats were grouped into four groups of seven rats reach: Group 1 (n = 7) was the control group to which no drugs were administered; this group was only provided with food and water. Group 2 (n = 7) was administered 20 mg/kg of methotrexate once intraperitoneally. Group 3 (n = 7) was administered 50 mg/kg of chrysin for 7 days orally. Group 4 (n = 7) was administered 20 mg/kg of methotrexate once intraperitoneally, followed by oral administration of 50 mg/kg of chrysin for 7 days. At the end of the experiment, rats were anaesthetised, rat testes were removed, and spermatozoon was obtained from the cauda epididymis. It was determined that sperm count and motility, glutathione peroxidase, superoxide dismutase and catalase activities decreased in the methotrexate group, whereas malondialdehyde, tumour necrosis factor-alpha, interleukin-1 beta and nuclear kappa factor B expression levels increased. Furthermore, damage to tubulus seminiferus structures and affusion in germ cells was identified. In the methotrexate + chrysin administered group, sperm count improved, biochemical enzyme levels increased, and structural improvements were observed in testicular tubules. These findings demonstrated that chrysin plays a protective role in testicular damage in rats.
Effectiveness of Hesperidin on Methotrexate-Induced Testicular Toxicity in Rats
(Kafkas Univ, veteriner Fakultesi dergisi, 2017) Belhan, Saadet; Ozkaraca, Mustafa; Kandemir, Fatih Mehmet; Gulyuz, Fetih; Yildirim, Serkan; Omur, Ali Dogan; Yener, Zabit
The aim of this study was to investigate the effect of hesperidin on male reproductive system in rats to which methotrexate (MTX) was administered. In the study, 28 male Wistar albino rats at the age of 8 weeks and had 250-300 g of live weight were used. Four experimental groups were formed; Group 1 (n=7): The control group, only feed and water were given. Group 2 (n=7): MTX group, a single dose of 20 mg/kg of i.p. MTX was administered. Group 3 (n=7): Hesperidin group, 200 mg/kg of hesperidin was administered by gavage for 7 days. Group 4: MTX + hesperidin group (n=7): Following administration of a single dose of 20 mg/kg i.p. MTX, 200 mg/kg of Hesperidin was administered by oral gavage for 7 days. At the end of the experiment, rats were decapitated and biochemical, histopathological and spermatological parameters were examined. It was observed that in the MTX group, sperm motility and density, the enzymes CAT, GPx and SOD and GSH level decreased, TNF-alpha and IL-1 Beta, as well as MDA, levels were increased, regular structure of spermatogenic cells was impaired, and seminiferous tubules became necrotic and degenerative. It was determined that spermatological parameters improved and, necrotic and degenerative changes diminished by the administration of MTX+hesperidin. These outcomes indicated that hesperidin had a protective effect on destructive effects of MTX in rat testiclesv.
Effects of Melatonin on the Onset of Ovarian Activity in Turkish Van Cats
(Medwell online, 2009) Gulyuz, Fetih; Tasal, Ibrahim; Kaya, Mustafa; Uslu, Baris Atalay
With the present study, we aimed to investigate the effects of melatonin on the initiation of breeding season in the female cats. For this purpose, 12 female cats and 2 tom-cats (male cats) (vasectomized) at age of 2-6 years old were used. The female cats were randomly divided into control (group 1, n = 6) and melatonin-treated groups (group 2, n = 6). Sham (placebo) and melatonin implants (containing 18 mg of melatonin) were placed subcutaneously into the females during the late anoestrous in groups 1 and 2, respectively. Sexual behaviors, vaginal smears and serum progesterone levels for the animals were recorded. The effect of the daylight (varying from 9.5-12.5 h, December through April) on the cyclic activity were also assessed during the study period. The animals were sexually silent during the anoestrus period. Upon the commencement of breeding season, while the female cats in group I showed estrus signs, none but one of the female cats in group 2 possessed the indications of the estrus. Moreover, the findings of cytology were not statistically different in both groups during the study period (p >= 0.05). Although, the progesterone levels in breeding season were higher in group 1 (p <= 0.05) than in un-breeding season, no such difference was present in group 2. The progesterone levels in breeding season were markedly elevated (p <= 0.01) in group I with comparison to group 2. In the latter group, melatonin effectively postponed cyclic activity of the females. The present results suggest that the administration of exogenous melatonin might prolong un-breeding season, presumably via the suppression of hypothalamic centers. Besides, we found that exposure to daylight of 10.5 or more hours per day were also, sufficient for the onset of cyclic activity in the female cats.
Effects of Oestrus Synchronisation Using Melatonin and Norgestomet Implants Followed by Ecg Injection Upon Reproductive Traits of Fat-Tailed Morkaraman Ewes During Suckling, Anoestrus Season
(Elsevier, 2012) Uslu, Baris Atalay; Tasal, Ibrahim; Gulyuz, Fetih; Sendag, Sait; Ucar, Omer; Goericke-Pesch, Sandra; Wehrend, Axel
The aim of this study was to compare the efficacy of different oestrus synchronisation protocols using melatonin (MEL), norgestomet (NOR) and melatonin + norgestomet (MELNOR) treatments (further, all animals from the groups MEL, NOR and MELNOR were supplemented with equine chorionic gonadotropin, eCG) in fat-tailed Morkaraman ewes during the non-breeding season. Forty healthy suckling ewes were assigned, from mid-May onwards, randomly to one of four experimental groups. Ten ewes were assigned to the control group CON (n = 10) and received no treatments. Ten ewes were assigned to the MEL group (n = 10) receiving a subcutaneous melatonin ear implant for 35 days, followed by a 500 IU intramuscular (i.m.) injection of eCG. Ten ewes were assigned to the NOR group (n = 10) and received norgestomet implants placed subcutaneously in the ear for 10 days and followed by a 500 IU i.m. injections of eCG after removal of the implant. The last 10 ewes were assigned to the MELNOR group (n = 10) receiving a subcutaneous melatonin ear implant for 35 days plus a subcutaneous norgestomet ear implant, inserted on the 25th day of the melatonin implant, for 10 days. It was then followed by a 500 IU i.m. injection of eCG after removal of the norgestomet implant and by introduction of rams to the flock concurrently. Ewes exhibiting oestrous signs by 7 days were hand-mated with rams known to be fertile. Blood samples were collected at the time of first implantation (baseline), on day 35 (norgestomet implant removal and eCG injection) and when ewes expressed oestrus in order to determine plasma progesterone (P-4) concentrations. The values of oestrus response, onset of oestrus post-treatment, pregnancy/lambing rates and litter sizes were recorded. The results showed that the oestrus rates were identical for all synchronisation protocols (100%) and the oestrus rates (40 +/- 16.3%) were significantly lower (P < 0.001) in the control group. The onset of oestrus shortened slightly in the hormone-treated groups. The P-4 concentrations on the day of eCG injections (at the end of synchronisation) were significantly higher (P < 0.01) in the hormone-treated groups (varying from 2.45 to 2.91 ng/ml) compared to the control group (0.45 ng/ml). Pregnancy/lambing rates were slightly higher in the synchronised groups (varied from 60 to 80%), compared to the control group. The results of oestrus synchronisation with melatonin and norgestomet ear implants (supplemented with the eCG) showed that: (i) an oestrus onset could be shortened significantly (P < 0.001) with melatonin and/or norgestomet, and (ii) significantly higher (P > 0.01) P-4 concentrations and higher lambing rates could be obtained in all hormone-treated ewes when compared to the un-treated Morkaraman ewes during the suckling (anoestrus) season. (C) 2012 Elsevier B.V. All rights reserved.
Electroejaculation in Impotent Rams and Artificial Insemination in Ewes
(indian veterinary Journal, 2006) Kaya, Mustafa; Gulyuz, Fetih; Uslu, Baris Atalay; Tasal, Ibrahim
This research was carried out to evaluate the efficiency of semen collection by the electroejaculation, method from Kivircik impotent rams and Al using fresh semen in Akkaraman ewes.
Estrus Synchronisation With Prid and Calving Rate in Cows
(indian veterinary Journal, 2006) Kaya, Mustafa; Gulyuz, Fetih; Uslu, Baris Atalay
The objective, of the. study was to determine best time: for Al, the effect of. timing of Al on calving raties-and to improve of pregnancy rates after estrus synchronization with PRID in cow and heifers in natural condition.
Is There a Relationship Between Serum Minerals (Ca, Mg) and Trace Elements (Cu, Fe, Mn, Zn) at Mating on Pregnancy Rates in Fat-Tailed Morkaraman Sheep
(Agricultural Research Communication Centre, 2017) Uslu, Baris Atalay; Mis, Leyla; Gulyuz, Fetih; Comba, Bahat; Ucar, Omer; Tasal, Ibrahim; Wehrend, Axel
The specific roles of nutrients in reproduction are not well defined in sheep. Hence, the relationships between certain mineral (Ca, Mg) and element (Cu, Fe, Mn, Zn) levels at mating and pregnancy rates was investigated in fat-tailed Morkaraman ewes synchronised with different hormones (melatonin, norgestomet implant and eCG) early in anoestrus season. For this purpose, 40 healthy ewes (aged 2-4 years) allocated in the synchronised (n=30) and no-treated group (n=10) were used during mid-May (suckling period). On the eCG injection day, fertile rams (n=8) were introduced into the groups and, then the oestrus signs were visually monitored twice daily for 7 days. The oestrous ewes were allowed to hand-mate using rotational (four rams daily) mating system. Additionally, jugular blood samples were collected on the days of onset of oestrus (before mating) for determination of serum Ca, Cu, Fe, Mg, Mn and Zn levels. Presence of pregnancies was diagnosed by real-time ultrasonography 35 days post-mating. Totally, 25 ewes became pregnant (21 ewes with stimulated-and 4 with spontaneous oestrus), while 9 ewes (all received stimulation) were not pregnant. In non-pregnant ewes, unlike other minerals and elements, the Cu and Zn values were significantly lower than their reference values (for Cu: 0.87 +/- 10.16 mg/dl vs. 9.20 mg/dl; for Zn: 2.06 +/- 10.30 mu g/dl vs. 3.8 mu g/dl, resp.; P<0.01 in both cases). In pregnant ewes, the corresponding values remained unchanged (P>0.05). Hence, the present findings indicate that the low Cu and Zn levels during the oestrus might adversely affect the subsequent pregnancy rate in suckling ewes.
A Retrospective Examination of Reproduction and Survival Rates of Van Cats
(Agricultural Research Communication Centre, 2017) Cak, Bahattin; Uslu, Baris Atalay; Demirel, Ahmet Fatih; Gulyuz, Fetih; Yilmaz, Orhan; Kosal, Volkan
Van cats are animals that often become the centre of attention due to their white hair, colourful eyes, and affectionate attitudes. However, it is nearly impossible to find research studies on the reproductive behaviours of Van cats. We conducted this study with the purpose of revealing the breeding efficiency properties and survival rates of Van cats. The study materials consisted of Van cats in the Van Cat Home of Yuzuncu Yil University's Van Cats Research Center. We determined the breeding efficiency properties using records obtained from 56 female cats over the course of four years. As a result of the study, we determined that the durations of oestrus, pregnancy, and lactation in Van cats were, respectively, 2.41 +/- 0.09, 63.75 +/- 0.27, and 98.45 +/- 1.18 days. We also determined the average number of kittens per delivery and the mating per pregnancy as, respectively, 3.30 +/- 0.19 and 13.71 +/- 0.74. In this study, we recorded the presence of 56 delivering cats, with a total of 185 kittens, 22 of which were dead (11.89%), and an 88.11% survival rate (%). The most common delivery type was twin deliveries (30%), and the highest rate of stillbirths was 4.86% in quadruplet deliveries.
Short-Acting Deslorelin Implant (Ovuplant®) Could Not Sufficiently Induce Fertile Cycles of Coloured Mohair Goats in Suckling (Anoestrous) Season
(Kafkas Univ, veteriner Fakultesi dergisi, 2012) Uslu, Baris Atalay; Sendag, Sait; Tasal, Ibrahim; Gulyuz, Fetih; Ucar, Omer; Wehrend, Axel
It is known that the pituitary FSH and LH stores are dramatically depleted during the anoestrous in seasonal breeders. Hence, the aim of this study was to evaluate the effect of deslorelin implant (a GnRH analogue) to induce oestrus in suckling goats. Suckling goats (n=21) of coloured Mohair breed were randomly assigned into two trial groups: Goats in Group I (n=11, Ovuplant (R)) received subcutaneously the implant (2.1 mg deslorelin), while animals in Group II (n=10, control) received no treatment. Along with the implant administration, serum progesterone (P-4) concentrations were determined (every other day up to 12 d) by RIA method. Within 4 days of implant administration, there were significantly higher (P<0.001) rates (100%) of pro-oestrus signs (attractiveness without mating) in Group I, than those in controls (zero %). The signs sustained for 9 days without oestrus (mating) in the vast majority (10/11) of implant-treated animals. But, an exceptional goat was in receptive oestrus commenced from the third day following the onset of pro-oestrus. Even so, there was no pregnancy post-mating. The P-4 concentrations between the groups remained below 1 ng/ml, regardless of implant treatment. Findings suggest that; i) for the P-4 concentrations, there was no marked difference between implant-treated and control groups, as remaining below 1 ng/ml all, and ii) short-acting deslorelin implant could not induce fertile oestrus cycles in coloured Mohair goats during the suckling (anoestrous) season.