Browsing by Author "Gunduz, Suna Gul"

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Effects of Dietary Nucleotide Yeast on Immune Responses and Antioxidant Enzyme Activities of Rainbow Trout Juveniles (Oncorhynchus Mykiss)
(Israeli Journal of Aquaculture-bamidgeh, 2016) Ozluer-Hunt, Arzu; Ozkan-Yilmaz, Ferbal; Berkoz, Mehmet; Engin, Kenan; Gunduz, Suna Gul; Yalin, Serap
This study aimed at demonstrating the effects of dietary supplementation of nucleotide yeast base protein (Nu-Pro (R)) (NP) on the antioxidant enzyme activities and immune response in liver and blood tissues of rainbow trout (Oncorhynchus mykiss). Fish with an average initial weight of 27.75 +/- 0.26 g were randomly assigned to four groups with three replicates. Throughout the 60 day grow-out period the control group was fed a fish meal based basal diet, and three other groups were fed diets in which 20 (NP 20), 40 (NP 40) and 60 % (NP 60) fish meal was substituted with nucleotide (Nu-Pro (NP) yeast). There were no significant changes of superoxide dismutase (SOD) and catalase (CAT) activities in liver among the experimental groups. A significant decrease (P<0.05) in malondialdehyde (MDA) level of tissue was observed in all nucleotide supplemented groups when compared to the control group. Serum lysozyme (LYZ) and myeloperoxidase (MPO) activities and nitric oxide (NO) level of liver tissue were significantly (P<0.05) increased in fish fed with nucleotide yeast based protein diets. The results showed that the fish in all nucleotide supplemented groups showed significantly better antioxidant activity and immune responses.
Effects of Dietary Selenium of Organic Form Against Lead Toxicity on the Antioxidant System in Cyprinus Carpio
(Springer, 2014) Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Gunduz, Suna Gul; Berkoz, Mehmet; Yalin, Serap
In this study was evaluated potential protective effect of organic selenium (Se) on heavy metal stress induced by lead (Pb) in Cyprinus carpio. For this reason, C. carpio was exposed to sublethal concentration of Pb (1.5 mg/L Pb(NO3)(2)) for 14 days. The fish were fed a basal (control; measured 0.55 mg/kg Se) diet or a basal diet supplemented with 2.50 mg/kg (measured 2.92 mg/kg Se) organic Se (Sel-Plex(A (R))) during the experiment period. The variations in glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) activities, and levels of reduced glutathione (GSH) with malondialdehyde (MDA) in liver and brain tissues of C. carpio were investigated in experimental groups. GSH levels in liver and brain tissues were significantly decreased by exposure to Pb. GST activity was significantly increased (p < 0.05) in liver tissue, but decreased in brain of treated fish by exposure to Pb. Also, GSH-Px activity was significantly increased in liver tissue, but decreased in brain of Pb-treated fish. Levels of MDA were increased in liver and brain of Pb-treated fish. The organic Se treatment for Pb-intoxicated animals improved activities of GSH-Px, GST and levels of MDA within normal limits. Supplemented Se could be able to improve Pb-induced oxidative stress by decreasing lipid peroxidation and regulating antioxidant defense system in tissues.
Effects of Fenbutatin Oxide on Antioxidant System of Different Tissues in Cyprinus Carpio (L., 1758)
(Parlar Scientific Publications (p S P), 2017) Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Gunduz, Suna Gul; Berkoz, Mehmet; Yalin, Serap; Yildirim, Metin
In this study, effects of sublethal concentrations of fenbutatin oxide on catalase (CAT) and superoxide dismutase (SOD) activities and lipid peroxidation in muscle, liver, kidney and brain tissues of Cyprinus carpio were investigated. The 96-hours LC50 value for fenbutatin oxide was determined as 1.544 mg/L for C. carpio in this study. 0.15 mg/L (1/10 of LC50) and 0.30 mg/L (1/5 of LC50) sublethal concentrations were applied for 96 h (4 days) in this experiment. The CAT activities in tissues were increased relation to doses applied. The SOD activities were decreased by 0.30 mg/L. The tissue MDA levels were significantly increased in relation to dose applied.
The Effects of Fish Meal Replacement by Yeast Based Nucleotides on Growth, Body Composition and Digestive Enzyme Activity in Rainbow Trout Juveniles (Onchorchyncus Mykiss)
(Israeli Journal of Aquaculture-bamidgeh, 2014) Hunt, Arzu Ozluer; Yilmaz, Ferbal Ozkan; Engin, Kenan; Berkoz, Mehmet; Gunduz, Suna Gul; Yalin, Serap; Sahin, Nefise Ozlen
This 60 day study investigated the effects of organically certified nucleotide yeast-derived protein source (Nu-Pro (R)) on growth, feed efficiency, fillet proximate composition and digestive enzymes in rainbow trout (Onchorhyncus mykiss). Diets were isonitrogenous and isocaloric. Three experimental diets wherein 20%, 40% and 60% of the fish meal content was replaced by Nu-Pro (R), were compared to the control diet in which the crude protein content was anchovy fish meal and corn gluten meal. The rainbow trout (initial weight 27 g/fish) were fed twice daily in 200-l Aqaria. Live weight gain increase ranged from 125-195% in fish fed the experimental diets. Results indicated that up to 40% fish meal protein can be replaced by Nu-Pro (R) without compromising growth rates, feed efficiency or the fillet biochemical composition in the rainbow trout. The effect of the dietary Nu-Pro (R) supplementation on digestion was partly observed by assaying the activity of pepsin, intestinal amylase, trypsin and lipase. Apart from amylase activity, results indicated that dietary supplementation of nucleotides is beneficial and may also have differential effects upon digestive enzyme activities.
Effects of Methidathion on Antioxidant System and Expression of Heat Shock Protein 70 (Hsp70) Gene in the Liver of Oreochromis Niloticus L. 1758
(Parlar Scientific Publications (p S P), 2015) Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Gunduz, Suna Gul; Berkoz, Mehmet; Yalin, Serap; Sahin, Nefise Ozler
In this study, effects of sublethal concentrations of methidathion on catalase (CAT), superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) activities and lipid peroxidation with expression of heat shock protein (HSP70) gene in liver tissues of Oreochromis niloticus were investigated. The 96-h LC50 value for methidathion was determined as 0.1045 mg/L for O. niloticus in this study. 0.013 (1/8 of LC50) and 0.026 mg/L (1/4 of LC50) sublethal concentrations were applied for 4 and 10 days in this experiment. CAT and GSH-Px activities in liver tissue were increased, in relation to both the time and doses applied. SOD activities were increased under the influence of both concentrations at the 4th day, but on 10th day, it was decreased with influence of 0.026 mg/L methidathion concentration. The tissue MDA levels were significantly increased in relation to both the time and dose applied. The relative mRNA levels of the HSP70 gene were detected by real time polymerase chain reaction (RT-PCR). The mRNA levels of HSP70 gene by methidathion increased significantly on 4th days compared to control, but decreased significantly on 10th days.
Influence of Sublethal Chlorpyrifos Exposure on Oxidative Stress and Acetylcholinesterase Activity in Common Carp (Cyprinus Carpio)
(Parlar Scientific Publications (p S P), 2019) Berkoz, Mehmet; Ozkan-Yilmaz, Ferbal; Ozluer-Hunt, Arzu; Gunduz, Suna Gul; Yildirim, Metin; Yalin, Serap
The commonly used pesticides in agriculture cause enzyme inactivation and DNA damage via reacting with macromolecules and may also initiate peroxidation of poly-unsaturated fatty acids (PUFA) by generating of the reactive oxygen species (ROS), as a consequence they can lead to the oxidative stress. The objective of this study was to determine the oxidative and neurotoxic potential of sublethal chlorpyrifos in the brain tissue of Cyprinus carpio (C. carpio) for a period of 96 and 240 hours, respectively. Therefore, the levels of malondialdehyde (MDA), glutathione (GSH), advanced oxidation protein products (AOPP), protein carbonyl, and 8-hydroxydeoxyguanosine (8-OHdG) levels and activities of superoxide dismutase (SOD), catalase, glutathione peroxidase (GSH-Px) and acetylcholinesterase (AChE) were investigated in order to determine the neurotoxic effect in brain tissue of C. carpio exposed to chlorpyrifos. Administration of chlorpyrifos at 0.26 and 0.52 mg/L concentrations caused a significant increase in MDA levels at the 240th hour (p<0.05), the latter concentration increased the GSH level but decreased SOD, GSH-Px, and CAT activities at the 240th hour (p<0.05). Only 0.52 mg/L of chlorpyrifos administration increased protein carbonyl but not AOPP levels at the 240th hour (p<0.05). 0.26 and 0.52 mg/L of chlorpyrifos concentrations caused a significant increase 8-OHdG levels at the 96th and the 240th hours (p<0.05). Chlorpyrifos statistically significantly reduced the AChE levels in both periods and at all administered concentrations (p<0.05). We observed an enhanced oxidative stress and inhibited AChE activity in the brain tissue of C. carpio after exposure to chlorpyrifos. These findings show that sublethal concentration of chloropyrifos leads to significant toxicity in the brain tissues of C. carpio.