Browsing by Author "Guney, Mehtap"

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Comparison of Microbial Protein Synthesis and Nutrient Digestibility of Medicago Sativa and Prangos Pabularia Hay in Sheep
(Agricultural Research Communication Centre, 2018) Guney, Mehtap; Erdogan, Sibel; Karsli, Mehmet Akif; Demirel, Murat
The aim of this study was to compare feed intake, nutrients digestibility, protein fractions entering into the duodenum, and ruminal fermentation parameters of sheep fed Medicago sativa (alfalfa) and Prangos pabularia, locally called kerkol hays. In the study three ruminally and duodenally cannulated Morkaraman sheep were used. It was carried out as two periods within a 2x3 crossover experimental design. Daily intake of crude protein (CP) was higher (P< 0.05) in sheep fed alfalfa than those fed kerkol hay. Digestibility of NDF and ADF were lower (P< 0.05) in sheep fed alfalfa hay than those fed kerkol hay (P< 0.05). The percentage of protein escaping ruminal degradation was higher (P< 0.05) in sheep fed kerkol hay (30.73%) than those fed alfalfa hay (15.05%). Ruminal fermentation parameters were similar between groups, except ruminal NH3-N concentration. It is concluded that kerkol hay can be used as forage for sheep feeding.
Determination of Fermentation Properties and Digestibility Characteristics of Combination of Corn-Soybean and Corn Silages
(Medwell online, 2009) Demirel, Murat; Celik, Sibel; Temur, Cuneyt; Guney, Mehtap; Celik, Savas
This study was conducted to determine in vivo digestibility and fermentation property of various silages made of green herbage of Corn (C) and corn-soybean mixtures at different rates (90% corn + 10% soybean (90C10SB), 80% corn + 20% soybean (80C20SB), 70% corn + 30 soybean (70C30SB)). Each mixture was prepared at rates given above on fresh material basis and ensiled in 120 L plastic barrels (a total of 20, 5 replicate for 4 treatments) for 90 days. pH values of C silage were higher and acetic acid levels were lower than that of 80C20SB (p<0.05). Concentrations of lactic and propionic acid of corn-soybean mixture silages were found to be similar to C silage. Digestibility of DM, OM, ADF and NDF were higher in C silage than that of other silages, whereas digestibility of CP and EE of 80C20SB silage were higher than corn silage (p<0.05). It was concluded that ensiling corn with 20 or 30% soybean addition at green herbage basis generates desirable outcomes for fermentation properties and digestibility of crude nutrients.
Determination of the Yield Characteristics and in Vitro Digestibility of Barley Forage Harvested in Different Vegetation Periods
(Agricultural Research Communication Centre, 2016) Guney, Mehtap; Kale, Cagri; Bolat, Duran; Deniz, Suphi
This study planned to determine the differences among nutrient composition, in vitro digestibility, energy content, digestible dry matter and organic matter yields of barley forage harvested at three different stages of maturity. Each vegetation period (heading stage, seed formation stage and mature stage) was randomly assigned to 5 replication from 1 square meter area and fifteen samples were harvested in total. DM, ADF (p<0.001), and NDF (p<0.05) contents were different in each stages of barley forage. In vitro dry matter (IVDMD), organic matter digestibility (IVOMD), metabolizable energy (ME), and net energy for lactation (NEL) values of samples were determined to be lower than the other two stages at the mature stage (p<0.05). Yield parameters of barley were significantly affected by vegetation period (p<0.001). It can be concluded that all three vegetation period had significantly higher digestibility. Digestible DM, OM and energy yields were higher when harvested at the mature stage of vegetation.
Does the Length of Time Dried Distillers' Grain With Solubles Substitution for Soybean Meal Affect Physiological Indicators and Meat Quality in Finishing Lambs
(Wiley, 2021) Karaca, Serhat; Erdogan, Sibel; Guney, Mehtap; Cakmakci, Cihan; Saribey, Muammer; Kor, Askin; Ulker, Hasan
This study examined how inclusion of dried distillers' grains with solubles (DDGS) in finishing lamb diets for different periods affects some physiological indicators and meat quality. A total of 40 male lambs were divided into four groups according to feeding regimen during the 120-day finishing period as follows: C120: no DDGS included in diet for 120 days; D120: DDGS included in diet for 120 days; D75: no DDGS included in diet for 45 days + DDGS included in diet for 75 days; D45: no DDGS included in diet for 75 days + DDGS included in diet for 45 days. Dietary inclusion rate of DDGS was 27.5%. Fattening performance and rumen parameters were not affected by treatment. Feeding regimens had no significant effect on meat quality except the instrumental tenderness and juiciness score. DDGS fed lambs had higher level of total trans fatty acids and n-6/n-3 ratio in meat. 10t-C18:1, 11t-C18:1 and 9c,11t conjugated linoleic acid contents of adipose tissue were higher in all the DDGS groups compared with C120 lambs (p < .001). These results suggest that soybean meal can be replaced with corn DDGS in lamb diets for up to 120 days during the finishing period with no adverse effects on some physiological response feedlot performance and meat quality.
Effect of Dried Corn Distillers' Grains With Solubles and Soybean Meal Supplements on Physiological Indicators and Reproductive Performance of Ewes
(Taylor & Francis Ltd, 2018) Erdogan, Sibel; Karaca, Serhat; Guney, Mehtap; Kor, Askin; Cakmakci, Cihan; Saribey, Muammer
This study aimed to evaluate the effects of adding dried distillers' grains with solubles (DDGS) to the nutritional regimens of ewes at different time periods on reproductive traits, serum hormones and serum metabolites. In Experiment 1, 100 ewes were divided into 4 groups (n=25) according to diet. The four diets were grass hay (H) alone (group H), H with barley (group H+B), H with DDGS for the whole feeding period (27d) (group H+DDGS) or H and DDGS for 5d (d 8-12 of oestrus cycle) (group H+DDGS5). Serum progesterone concentrations were affected by oestrus cycle (p<0.001), but not by dietary treatments. However, feeding H+DDGS caused significant increases in serum insulin, leptin and growth hormone concentrations (p<0.05). In Experiment 2, 30 ewes were divided into 2 groups (n=15), receiving DDGS or soybean meal (SBM) during the prepartum period. Diets had no significant effect on weights of dams or lambs at birth; however, the weaning weights of lambs born from ewes of group DDGS were significantly higher (p<0.05). Moreover, serum glucose, blood urea nitrogen, triglyceride and beta hydroxybutyric acid (BHBA) concentrations during the prepartum period were affected by dietary treatments and by time (p<0.001). During the prepartum period, BHBA concentrations of ewes fed SBM were higher, while BHBA levels decreased during the last weeks of pregnancy regardless of diet. Lamb serum immunoglobulin G concentrations increased from 1h to 24h after birth (p<0.001). Colostrum of ewes fed SBM had higher fat-free dry matter (DM) and protein contents in comparison to colostrum of ewes fed DDGS (p<0.05). In conclusion, DDGS can be included as protein source in pregnancy rations up to 15% of DM to obtain reproductive performance outcomes equal to or exceeding those obtained with SBM.
Effect of Grain Processing at Different Barley Varieties on Nutrient Compositions, Starch Contents and in Vitro Digestion Parameters
(Mattioli 1885, 2019) Guney, Mehtap
This study examined how response of treating with heat of twelve different barley varieties on nutrient composition, starch contents, in vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD). In the study, a total of twelve different barley varieties (Tarm-92, Avci-2000, Cetin-2002, Ozdemir, Ince, Bolayir, Anka-04, Tosunpasa, Larende, Marti) were used. Processing with heat to all barley varieties were significantly higher contents of DM, OM and neutral detergent fiber (NDF). However, starch contents were significantly lower compared to unprocessing group (p<0.05). Treating with heat in term of IVDMD and IVOMD had importantly decreased in some varieties, while no change did in some varieties (p<0.05). Of all results, it was thought that treating with heat of barley varieties were important in term of regulating synchronization of energy and protein in rumen of nutrient digestion in ruminant as it increase the cell wall.
The Effect of Molasses and Pre Fermented Lactic Acid Juice on Silage Quality and in Vitro Digestibility of Sugar Beet Pulp Plus Wheat Straw Silage
(Parlar Scientific Publications (p S P), 2018) Guney, Mehtap; Kale, Cagri; Temur, Cuneyt; Bingol, Nuriye Tugba; Aksu, Taylan
In the present study, the effect of some silage additives (molasses, pre-fermented lactic acid juice and their combination) on silage quality of sugar beet pulp+wheat straw mixture was investigated. For this purpose, 4 groups, each consisted of 6 replicates, were designed; 1) sugar beet pulp+wheat straw mixture (Control), 2) sugar beet pulp+wheat straw mixture plus 2% molasses (M), 3) sugar beet pulp+wheat straw mixture plus pre-fermented lactic acid juice (PFJ, 44.07x10(7) cfu/ml(-1) LAB) and, 4) sugar beet pulp+wheat straw mixture plus 2% molasses+pre-fermented lactic acid juice (M+PFJ). The silages prepared in 1 L glass jars were opened after 60 days. While the amounts of dry matter (DM), crude ash (CA), and neutral detergent fiber (NDF) of treated-silages were higher than those of the control group (P <0.05), crude protein (CP) levels were not affected by treatment. pH, Fleigh score (FS), and lactic acid amounts were not affected by additives. However, the addition of molasses and PFJ decreased the NH3-N concentration of the silages compared to the control group (P <0.05). The M+PFJ addition decreased the acetic and propionic acid concentrations of the silages compared to those of the control group (P <0.05). As a result, the addition of 2% molasses and/or PFJ to the sugar beet pulp+wheat straw mixture reduced the DM losses of silages and did not affect the silage fermentation parameters and, in vitro digestibility of dry matter (DMD) and organic matter of silages (OMD).
Effect of Various Additives and Harvesting Stages on Rumen Degradation of Sunflower Silages
(Taylor & Francis Ltd, 2009) Demirel, Murat; Bolat, Duran; Eratak, Serdar; Celik, Sibel; Bakici, Yunus; Celik, Savas; Guney, Mehtap
To study effective degradability (nylon bag technique) of sunflower silage, as influenced by harvesting stage (blooming, milk or dough) or molasses (m) / urea (u) mixture, seven different silage samples for each harvest stage [sunflower (SF) and 6 mixtures (2%mSF, 4%mSF, 2%m+0.5%uSF, 2%m+1%uSF, 4%m+0.5%uSF, 4%m+1%uSF)] were incubated for 70 days in 1 litter jars. The DM and CP effective degradation of SF silage was found to be similar at blooming and milking stage and higher than that of dough stage (P < 0.05). The effective degradation of ADF and NDF of SF silage at milking and dough stages was lower than that of blooming stage. The positive effects of molasses and molasses+urea additives on effective degradability were observed. It was concluded that decreased degradability of nutrient matters because of its advanced vegetational stage could be partly compensated by using different amount of molasses and molasses+urea additives.
Effects of Dietary Supplementation With Rosemary Oil on Methanogenic Bacteria Density, Blood and Rumen Parameters and Meat Quality of Fattening Lambs
(Taylor & Francis Ltd, 2021) Guney, Mehtap; Karaca, Serhat; Erdogan, Sibel; Kor, Askin; Kale, Cagri; Onalan, Sukru; Bingol, Nuriye Tugba
This study aimed to determine the effect of rosemary (Rosmarinus officinalis) essential oil (REO) in lamb fattening diets on blood, rumen parameters, fattening performance and meat quality. Thirty Norduz male lambs weaned at 4 months of age with average body weight 22.0 +/- 4.41 kg were used. Lambs were divided into three groups: no rosemary oil (control, R0), with 250 mg/kg DM (R250) and with 500 mg/kg DM (R500) rosemary oil added to the basal ration, and fed for 70 d. The daily feed intake of the R500 lambs (1.63 kg) was lower than other groups (1.70 kg) (p = .01). Rosemary oil did not change the density of methane-producing bacteria in the rumen fluid. Rumen pH was 6.31 in R250 lambs, while it was 6.16 in control (p < .04). The proportion of propionic acid (PA) increased in R250 (26.5 molar%) and R500 (26.0 molar%) lambs compared to control lambs (22.7 molar%) (p < .001). Serum glucose levels increased with REO dose (p < .01) and serum IGF-1 levels were significantly higher in R250 lambs (p < .001). The dose rates of REO used in fattening lambs had limited effect on fattening performance, carcase and meat quality. The results showed that although it does not affect the final live weight, the negative effect of REO on feed intake at 500 mg/kg was considered as a limiting factor and 250 mg/kg dose of REO may have positive effects on ruminal fermentation. Hence, it may be beneficial to try doses lower than 500 mg in further studies.
The Effects of the L. Plantarum Strain Rc1.4 Starter Culture With or Without Sucrose Addition on Fermentation Efficacy, Microbial Content, and Aerobic Stability Indicators of Alfalfa Silage
(Tubitak Scientific & Technological Research Council Turkey, 2021) Aksu, Taylan; Validov, Shamil; Karimova, Liliia; Demirel, Murat; Levendoglu, Taner; Erdogan, Sibel; Guney, Mehtap
To develop the approach for efficient alfalfa silage fermentation, L. plantarum strain RC1.4 (LP-RC1.4) was used as starter culture with or without sucrose. Trial groups consisted of ten repetitions for each treatment including (1) control, with added sterile water (10 mL); (2) LP, silages treated with LP-RC1.4 alone (10(6) cfu g(-1) FM); (3) LP-S 1, silages treated with LP-RC1.4 (10(6) cfu g(-1) FM) plus sucrose at 10 g/kg fresh matter; (4) LP-S-2, silages treated with LP-RC1.4 (10(6) cfu g(-1) FM) plus sucrose at 20 g kg(-1) fresh matter. Inoculation improved the quality of the silaging demonstrating a lower decrease of silage dry matter (DM) in comparison to the control silage. The CO2 production was also lower in the inoculated silages. The addition of sucrose improved microbiological and biochemical parameters of the resulting alfalfa silage, simultaneously with the increased number of lactobacilli, raised the content of water-soluble carbohydrates, while the number of yeasts and molds decreased. It was concluded that LP-RC1.4 inoculation should be supported with sucrose to insure domination of LAB fermentation and enhance of the silage quality of alfalfa.
Metagenomic Comparison of the Microbial Load Colonizing Lucerne Hay and Gum Astralagus (Astragalus Glycyphyllos) in Ruminants
(Csiro Publishing, 2025) Guney, Mehtap; Onalan, Sukru
Context. Rumen microbiota plays an important role in the evaluation of roughages. Gum astragalus hay is a non-traditional roughage in ruminant feeding, and it is curious how it affects rumen microbiota in comparison with lucerne hay (LH). Aim. This study compares the in vitro true digestibility and rumen fermentation parameters of lucerne hay (LH) and gum astralagus hay (Astragalus glycyphyllos, AG), as well as the metagenomic profile of the bacterial community in sheepruminal fluid following 48 h in vitro incubation with either forage type. Methods. The study was carried out in vitro in a Daisy incubator. A total of 100 samples, including 48 samples from each of the experimental roughages and two blind samples, were weighed into F57 bags for in vitro incubation with ruminal fluid, obtained from the rumen of two sheep fed on roughage and brought to the slaughterhouse from a private enterprise. Key results. Crude protein (CP) content of AG was higher (P < 0.0014) and ADF content (P < 0.001) was lower than LH. After 48 h incubation of roughage samples, AG was superior to LH in terms of in vitro true digestibility parameters (true digestion of roughages, true dry matter digestion, and true organic matter digestion; P < 0.001). Taxonomy results of bacteria colonizing both roughages were obtained at genus and species level, according to the Shannon and Simpsons index. The bacterial community of the genus Provetalla was dominant in both forages, whereas Pseudomonas genus bacteria were observed in AG, but not in LH. According to the total Operational Taxonomc Units (OTU) in the samples, it was observed that LH and AG had similarity, as well as diversity in terms of species. Due to the current shortage of roughage, it is very important to consider AG as an alternative source of roughage in animal nutrition. Conclusions. The differences in nutrient composition between the two roughage types also indicate differences in the composition and density of bacterial microbiota. These results were very important for improving the nutrient utilization efficiency of roughages in the desired direction. Implication. The nutrient composition and in vitro digestion parameter results of AG were comparable to LH, and there were similarities, as well as differences, in the colonized bacterial community in both roughages.