Browsing by Author "Gurturk, K."

Now showing 1 - 2 of 2

Distribution of Antiseptic Resistance Genes in Staphylococcus Spp. From Bovine Mastitis
(Czech Academy Agricultural Sciences, 2017) Ergun, Y.; Cantekin, Z.; Gurturk, K.; Solmaz, H.; Ekin, I. H.; Ozturk, D.
The purpose of this study was the determination of antiseptic resistance genes (qacA/B and qacC) from staphylococcal mastitis in cattle in various regions of Turkey. In total, 283 isolates (Burdur: 36, Hatay: 47 and Van: 200) were studied, and the antiseptic resistance genes were detected using simplex PCR. The distribution of the qacA/B and qacC genes, mediating resistance against quaternary ammonium compounds, was found to vary among the different isolates. The qacA/B genes were found in three of the Burdur isolates, six of the Hatay isolates and seven of the Van isolates. The qacC gene was found in two of the Burdur isolates, none of the Hatay isolates and two of the Van isolates. The presence of these genes and transmission among Staphylococcus spp. strains may pose risks in the control of mastitis, as well as to public health.
Western Blot Analysis of the Igg-Antibody Response To Acid-Glycine Antigens From Campylobacter Fetus Subsp. Fetus and C-Jejuni in Naturally Infected Sheep
(veterinarni A Farmaceuticka Univerzita Brno, 2007) Gurturk, K.; Ekin, I. H.; Arslan, A.
IgG-antibody response in aborting sheep and in apparently healthy sheep in a flock against acid-glycine-extracted antigens from three strains for each C fetus subsp. fetus and C jejuni were analysed by Western blot. One strain of C fetus subsp. fetus was isolated from aborting sheep. Western blot analysis of the sera revealed the presence of IgG antibody binding to the common antigens including proteins with the Mw of 63 kDa and 54 kDa in extracts from both C. fetus subsp. fetus and C. jejuni strains. In addition, IgG antibodies in sera from aborting sheep reacted more strongly with the antigens from C. fetus subsp. fetus strains with Mw of approximately 100, 95 and 86.5 kDa than those of apparently healthy sheep. The binding profile of the antibodies with these antigens appeared to be unique for each C. fetus subsp. fetus strain. On the other hand, IgG antibodies only in sera from aborting sheep recognized strongly the antigens of each C. fetus subsp. fetus strain at the Mw ranged from approximately 26 to 22 kDa. However, the antigenic components between 26 and 22 kDa were not detectable in coomassie blue stained gel and thought to have non-protein nature. These low molecular weight antigens of C. fetus subsp. fetus may be related to a recent infection in aborting sheep. These observations indicate that such species-specific antigens or conjugated protein antigens could be used for improving the specificity of the serological tests to detect C. fetus antibodies in sheep sera, and may be the candidates for subunit vaccines against ovine abortion.