Browsing by Author "Guven, Burcu"

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The Comparison of Antioxidant Capacity and Cytotoxic, Anticarcinogenic, and Genotoxic Effects of Fe@au Nanosphere Magnetic Nanoparticles
(Tubitak Scientific & Technological Research Council Turkey, 2017) Yegenoglu, Hande; Aslim, Belma; Guven, Burcu; Zengin, Adem; Boyaci, Ismail Hakki; Suludere, Zekiye; Tamer, Ugur
Magnetic gold nanoparticles are used in various biomedical, biochemistry, and biotechnology applications due to their controllable size distribution, long-term stability, reduced toxicity, and biocompatibility. Different coating materials, such as proteins, carbohydrates, lipids, and polyphenols, are applied to enhance the biocompatibility of nanoparticles. In this study, the effects of surface coatings of core-shell structured Fe@Au nanosphere magnetic nanoparticles with regard to antioxidant capacity and cytotoxic, anticarcinogenic, and genotoxic properties were investigated. The obtained results demonstrated that avidin-coated Fe@Au nanospheres had higher antioxidant capacities than uncoated nanospheres. Neither avidin-coated nor uncoated nanoparticles had a cytotoxic effect on normal cells (human gingival fibroblast cell line, HGF-1). In addition, they had anticarcinogenic effects on human cervical carcinoma (HeLa), human breast adenocarcinoma (MCF-7), and human colorectal adenocarcinoma (CCL-221). The genotoxic effects of nanoparticles were also evaluated with DNA tail damage ratio.
Effectivity of Pancreatic Enzyme Replacement Therapy in Malnourished Children
(Wiley, 2020) Guven, Burcu; Mis, Mevsim Demir; Karaman, Kamuran; Yasar, Akkiz Sahin
Objective: Malnutrition is commonly seen in children with exocrine pancreatic insufficiency (EPI). Pancreatic enzyme replacement therapy (PERT) is the mainstay treatment of acute malnutrition in children detected with a disease closely associated with EPI (eg, cystic fibrosis). The effectiveness of PERT in children with malnutrition without any chronic disease, however, remains unclear. The aim of this study was to investigate the effectiveness of PERT on weight gain and EPI in children classified as moderately and severely malnourished according to the World Health Organization (WHO) classification. Materials and Methods: The study included a total of 40 children aged 2-16 years who were classified as moderately and severely malnourished according to the WHO classification. The patients were randomly divided into 2 groups: PERT group (n = 20) received 2000 U lipase/kg/day (in 4 doses) in addition to hypercaloric enteral supplements and control group received hypercaloric enteral supplements only. In both groups, anthropometric measurements and the assessment of fecal elastase-1 (FE-1) levels were performed both at first admission and at the end of the 8-week treatment period. Results: On the basis of WHO classification, 10 (25%) children were classified as severely malnourished and 30 (75%) children as moderately malnourished. EPI was detected in all the patients, among whom 24 (60%) patients had severe EPI. At the end of the treatment, body weight, height, and body mass index (BMI) increased significantly in both groups compared to their pre-treatment values, whereas no significant difference was found with regard to waist circumference (WC) and FE-1 levels. Similarly, no significant difference was found between pre- and posttreatment measurements of PERT and control groups (P > 0.05) and between pre- and posttreatment measurements of patients with moderate and severe malnutrition (P > 0.05). Conclusions: Malnutrition remains a serious public health problem, and thus, the development of novel treatment methods is highly essential. PERT is one of the most commonly considered alternatives, although there is little documentation of PERT in the literature. In the present study, although PERT resulted in higher weight gain, it established no significant difference between the 2 groups.
Emotional Dysregulation in Adolescents With Functional Gastrointestinal Disorders
(Elsevier, 2020) Guven, Burcu; Gulerman, Fulya; Akyuz, Ebubekir; Aydin, Gozde
Background and Study Aims: In various gastrointestinal system diseases, emotional dysregulation has been shown to reduce pain tolerance and increase the severity of the disease. Increased emotional dysregulation during the adolescence period causes gastrointestinal symptoms to be more frequent and severe. In this study, Child Depression Inventory (CDI) scores were investigated in patients admitted to our clinic with functional gastrointestinal disorders. Patients and Methods: According to Rome IV criteria, 200 patients with functional abdominal pain and dyspepsia aged 12-17 years were included in this study. 100 patients without a chronic disease were taken as control group. Patients completed the self-report questionnaires about symptoms, school performance, nutrition and sports habits. We used Child Depression Inventory (CDI) to assess the patients' depression. Results: The mean age of study group was 15.29 +/- 1.48 years (12-17 years), median 16 years; 80% (160/200) were girls. The mean age of control group was 14.96 +/- 1.66 years (12-17 years), median 15 years; 70% (70/100) were girls. There is no difference between the two groups for age and gender. Median depression score was 12.5 (range, 0-53) in the study group and 10.0 (range, 0-41) in the control group and a significant difference was found between the two groups (p = 0.014). School performance was revealed as 'very good' in 112 (56%) children in the study group and in 24 (24%) children in the control group and a significant difference was found between the two groups (p < 0.001). A negative correlation was found between school performance and depressive symptoms. Conclusion: It is not clear that emotional dysregulation induces FGIDs or FGIDs cause emotional dysregulation. But it is known that these diseases are common in the adolescent age group. Incorporating social and physical activities into the educational processes of adolescents will have favorable effects on their academic performance as well as emotional regulation. (C) 2020 Pan-Arab Association of Gastroenterology. Published by Elsevier B.V. All rights reserved.
Fecal Elastase Levels in Children Diagnosed With Functional Abdominal Pain-Not Otherwise Specified
(Turkish J Pediatrics, 2021) Guven, Burcu; Turan, Nuriye Burcin; Oner, Ahmet Fayik; Karaman, Kamuran; Cetin, Mecnun
Background. Although the exact pathophysiology of functional gastrointestinal diseases remains unclear, numerous etiologies have been blamed, including visceral hypersensitivity, gastrointestinal motility disorders, psychological factors, intestinal mucosal inflammation, intestinal microbiota, and post-infectious syndromes. In the present study, we aimed to evaluate pancreatic insufficient patients diagnosed withfiinctional abdominal pain-not otherwise specified (FAP-NOS) according to Rome IV criteria. Methods. The study included a total of 110 patients aged 4-17 years who were diagnosed with FAP-NOS according to Rome IV criteria. The control group consisted of 80 patients with no gastrointestinal disorders and chronic diseases. Glucose, amylase, lipase, pancreatic amylase, immunoreactive trypsinogen (IRT) and fecal elastase (FE-1) levels were examined for each patient. Results. No significant difference was found between the two groups with regard to lipase, pancreatic amylase, IRT, and serum glucose levels. However, the amylase levels were significantly higher and the FE-1 levels were significantly lower in the study group compared to the control group (p=0.007 and p<0.001). The cut-off value detected in in ROC analysis for the diagnostic value of FE-1 in predicting FAP-NOS was found to be 140.107 mu g/g. Based on this value, the sensitivity, specificity, PPV, and NPV of FE-1 were 82.1%, 66.2%, 77%, 73%, respectively. Accordingly, the likelihood of FE-1 in providing a positive value in patients with FAP-NOS was almost 9 times higher than in individuals without FAP-NOS. Conclusions. FE-1 levels were significantly lower in children diagnosed with FAP-NOS and we consider that this difference could be attributed to malabsorption secondary to dysbiosis as there is not enough data.
Ganglioneuroma in a Child With Chronic Constipation and Abdominal Pain
(2018) Guven, Burcu; Avci, Veli; Beger, Burhan; Yaşar, Akkız Şahin
Ganglioneuromas are rare, benign, slow-growing tumors arising from the neural crest. They are most commonly in posterior mediastinum, retroperitoneum, adrenal medulla and neck. They are very rare in presacral region. A 15 year old girl presented with abdominal pain and chronic constipation. A mass was seen between the uterus and sacrococcygeal bone in abdominal CT. It is resected totally and evaluated as ganglioneuroma. Conclusion: Presacral ganglioneuroma is a benign tumor which is rarely encountered. Patients might present with abdominal pain and constipation complaints as seen in our patient. Therefore, imaging methods should certainly be used for patients with chronic constipation which is resistance to treatment.
Helicobacter Pylori Enfeksiyonu Olan Çocuklarda Tanı Testlerinin, Klinik ve Histopatolojik Bulguların Değerlendirilmesi
(2019) Gülerman, Fulya; Guven, Burcu; Kaçmaz, Birgül
Amaç: Ağır klinik tablo genelde erişkinlerde görülmekleberaber Helicobacter pylori (H.pylori) enfeksiyonu çocuklukçağında kazanılmaktadır. Bu da erken yaşta tanının öneminiarttırmaktadır. Bu araştırmanın amacı, çocuklarda H.pylorienfeksiyonunu yaş, cinsiyet, tanı testleri, klinik vehistopatolojik bulgularla değerlendirmektir.Gereç ve yöntem: Merkezimizde üst gastrointestinal sistem(ÜGS) endoskopisi yapılan ve biyopsi örneklerinde H. pylorisaptanan çocuk hastaların yaşları, cinsiyetleri, geliş şikayetleri,fekal H.pylori antijen sonuçları ve histopatolojik bulgularıretrospektif olarak değerlendirildi.Bulgular: Çalışmaya alınan 148 hastanın 111’i (%75) kız, 37’si(%25) erkekti. Yaş ortalaması 14.2 yıl (1-18 yıl, ortanca 15 yıl)idi. Hastaların %60,1’i karın ağrısı, %9,5’i bulantı, %2’si iseretrosternal yanma şikayeti ile başvurdu. H. pylori veinflamasyon yoğunluğu arasında pozitif korelasyon bulundu(p<0.001). Fekal H.pylori antijen testi duyarlılığı %21,4bulundu.Sonuç: Çocuklarda H.pylori enfeksiyonu, karın ağrısı ve bulantıgibi nonspesifik bulgularla görülebilmektedir. Tanı içininvaziv yöntemlere ihtiyaç vardır.
Helicobacter Pylori Resistance To Clarithromycin and Fluoroquinolones in a Pediatric Population in Turkey: a Cross-Sectional Study
(Wiley, 2019) Guven, Burcu; Gulerman, Fulya; Kacmaz, Birgul
BackgroundHelicobacter pylori antimicrobial resistance is gradually increasing around the world. However, there are a limited number of studies reporting on this issue in the pediatric population. In this study, we aimed to determine Hpylori resistance to clarithromycin and fluoroquinolones in the pediatric patients living in Krkkale province that were detected with Hpylori in gastric biopsies. Moreover, we also aimed to investigate the concordance between the histopathologic and molecular methods used in the diagnosis of Hpylori infection. Materials and MethodsPatients aged 2-18years who had a history of epigastric pain and/or nausea persisting for longer than 1month underwent upper gastrointestinal endoscopy. Biopsies were taken from the gastric antral mucosa. In the samples detected with Hpylori in the histopathologic examination, the presence of Hpylori and Hpylori resistance to clarithromycin and fluoroquinolones was investigated using the GenoType HelicoDR test which allows the detection of wild-type and mutant genes. The strains detected with more than one mutant gene are defined as hetero-resistant strains. ResultsThe 93 patients that underwent DNA extraction and amplification included 68 (73.1%) girls and 25 (26.9%) boys with a median age of 152.62 (range 6-17) years. The overall concordance for the diagnosis of Hpylori infection between histopathology and PCR was 94%, and Hpylori resistance to clarithromycin and fluoroquinolones was 27% and 15%, respectively. ConclusionsThe high Hpylori resistance to clarithromycin and fluoroquinolones among the pediatric patients in our region implicates that the antibiotic sensitivity of strains should be studied prior to administration in accordance with the recommendations provided in the guidelines. Moreover, the presence of hetero-resistant strains in our patients may be a reason for treatment failure.
Immunomagnetic Separation and Listeria Monocytogenes Detection With Surface-Enhanced Raman Scattering
(Tubitak Scientific & Technological Research Council Turkey, 2020) Akcinar, Hande Yegenoglu; Aslim, Belma; Torul, Hilal; Guven, Burcu; Zengin, Adem; Suludere, Zekiye; Tamer, Ugur
Background/aim: We aimed to develop a rapid method to enumerate Listeria monocytogenes (L. monocytogenes) utilizing magnetic nanoparticle based preconcentration and surface-enhanced Raman spectroscopy measurements. Materials and methods: Biological activities of magnetic Au-nanoparticles have been observed to have the high biocompatibility, and a sample immunosensor model has been designed to use avidin attached Au-nanoparticles for L. monocytogenes detection. Staphylococcus aureus (S. aureus) and Salmonella typhimurium (S. typhimurium) bacteria cultures were chosen for control studies. Antimicrobial activity studies have been done to identify bio-compatibility and bio-characterization of the Au-nanoparticles in our previous study and capturing efficiencies to bacterial surfaces have been also investigated. Results: We constructed the calibration graphs in various population density of L. monocytogenes as 2.2 x 10(1) to 2.2 x 10(6) cfu/mL and the capture efficiency was found to be 75%. After the optimization procedures, population density of L. monocytogenes and Raman signal intensity showed a good linear correlation (12 2 - 0.991) between 10(2) to 10(6) cfu/ml, L. monocylogenes. The presented sandwich assay provides low detection limits and limit of quantification as 12 cfu/mL and 37 cfu/mL, respectively. We also compared the experimental results with reference plate-counting methods and the practical utility of the proposed assay is demonstrated using milk samples. Conclusion: It is focused on the enumeration of L. monocytogenes in milk samples and the comparision of results of milk analysis obtained by the proposed SERS method and by plate counting method stay in food agreement. In the present study, all parameters were optimized to select SERS-based immunoassay method for L. monocytogenes bacteria to ensure LOD, selectivity, precision and repeatablity.
The Pattern of Tpeak-Tend Interval and Qtdis, and Pdis in Children With Brucellosis
(Oxford Univ Press, 2019) Cetin, Mecnun; Turfan, Nevzat; Karaman, Kamuran; Yasar, Akkiz Sahin; Guven, Burcu; Tuncdemir, Perihan
Objective: To investigate the effect of inflammation on the conducting system of the heart in patients diagnosed with brucellosis. Methods: A total of 42 patients diagnosed with brucellosis and 39 age and sex-matched healthy children were enrolled into the study. Pre- and post-treatment electrocardiographic parameters in brucellosis and control group were recorded from an electrocardiogram for each patient. Results: The results indicated that the parameters including Pmax, Pdis, QTmax, QTdis, QTcdis, Tp-edis interval and Tp-emax/QTmax and Tp-emax/QTcmax ratios, which are known to be key indicators for the prediction of severe atrial or ventricular arrhythmia and sudden cardiac death and also important parameters used as the indicators for the non-invasive evaluation of the transmural heterogeneity were significantly longer in the study group compared with the control group (p < 0.05). Conclusion: In this study, it was determined that the brucella disease had more cardiac involvement than thought, and this was more in the subclinical form of cardiac involvement.
Typhoid Fever Accompanied With Hematopoetic Lymphohistiocytosis and Rhabdomyolysis in a Refugee Child
(Lippincott Williams & Wilkins, 2019) Yasar, Akkiz Sahin; Karaman, Kamuran; Geylan, Hadi; Cetin, Mecnun; Guven, Burcu; Oner, Ahmet F.
Typhoid fever is an acute and systemic infectious disease that is specific to humans and is caused by Salmonella typhi. Patients may present with different findings ranging from simple fever to multiple organ failure. Hemophagocytosis and rhabdomyolysis are rare complications of typhoid fever. In this study, we presented a case of hemophagocytic lymphohistiocytosis and rhabdomyolysis in a 14-year-old girl who migrated from Mosul, Iraq, 1 month ago.