Browsing by Author "Hacimuftuoglu, Ahmet"

Now showing 1 - 3 of 3

Does Umbelliferone Protect Primary Cortical Neuron Cells Against Glutamate Excitotoxicity
(Kafkas Univ, veteriner Fakultesi dergisi, 2021) Demirkaya, Alper Kursat; Gundogdu, Gulsah; Karakaya, Songul; Yilmaz Tasci, Seymanur; Nalci, Kemal Alp; Hacimuftuoglu, Ahmet
Glutamate is the major excitatory neurotransmitter in the central nervous system. Excessive glutamate is known to cause excitotoxicity. Umbelliferone is a coumarin derivative compound and has antioxidant, anti-inflammatory, and neuroprotective effects. Also, umbelliferone can show neuroprotective effect by crossing the blood-brain barrier. In our study, it was aimed to investigate the neuroprotective effect of umbelliferone on primary cortical neuron (PCN) culture. Umbelliferone was isolated from the roots of Ferulago cassia dichloromethane sub-extract. The cerebral cortex of newborn Sprague Dawley rats was used to obtain PCNs. To stimulate glutamate excitotoxicity, cells were exposed to 6x10(-5)M glutamate. Then different concentrations (10-1000 mu M) of umbelliferone were added into the medium and allowed to incubate for 24 and 72 h. MTT assay was used to measure cell viability. Total Antioxidant Status (TAS) and Total Oxidant Status (TOS) analyzes were used to evaluate reactive oxygen species. MTT results showed that cell viability was decreased with glutamate application. 25-250 mu M umbelliferone had a significant protective effect against glutamate excitotoxicity at 72 h (P<0.05). Consistent with MTT results, TAS analysis results showed 50-250 mu M umbelliferone increase the level of antioxidants in cells, which can help protect neurons against glutamate-induced excitotoxicity. In this study, umbelliferone showed a neuroprotective effect in PCN against glutamate excitotoxicity. These results suggest that umbelliferone may be used as therapeutic agent against glutamate excitotoxicity.
Effect of Metformin/Irinotecan-loaded Poly-Lactic Acid Nanoparticles on Glioblastoma: in Vitro and in Vivo Studies
(Future Medicine Ltd, 2018) Taghizadehghalehjoughi, Ali; Hacimuftuoglu, Ahmet; Cetin, Meltem; Ugur, Afife Busra; Galateanu, Bianca; Mezhuev, Yaroslav; Abd El-Aty, A. M.
Aim: The present study was designed to evaluate the effects of irinotecan hydrochloride (IRI)- or metformin hydrochloride (MET)-loaded poly-lactic-co-glycolic acid (PLGA) nanoparticles (NPs) for the treatment of glioblastoma multiforme using in vitro neuron and U-87 MG glioblastoma cell cultures and in vivo animal model. Methods: The cytotoxic and neurotoxic effects of pure drugs, blank NPs and MET- and IRI-loaded PLGA NPs were investigated in vitro (using methylthiazolyldiphenyl-tetrazolium bromide assay) and in vivo (using Cavalieri's principle for estimation of cancer volume). Results: 1 and 2 mM doses of MET and MET-loaded PLGA NPs, respectively, significantly reduced the volume of extracted cancer. Conclusion: Consequently, MET- and IRI-loaded PLGA NPs may be a promising approach for the treatment of glioblastoma multiforme.
Evaluation the Effects of Helichrysum Plicatum Subsp. Pseudoplicatum on an In-Vitro Wound Model Using Human Dermal Fibroblast Cells
(Sage Publications inc, 2023) Miloglu, Fatma Demirkaya; Akpinar, Abdulbaki; Guven, Leyla; Demirkaya, Alper Kursat; Gundogdu, Gulsah; Nalci, Kemal Alp; Hacimuftuoglu, Ahmet
Wound is tissue damage that occurs in the skin. Helichrysum species (Altinotu) are rich in phenolic compounds used in traditional medicine for wound healing. The main component in their flower head (capitulum) is phenolic compounds. The present study investigates the proliferative, oxidative stress, and wound healing properties of the methanolic extract of Helichrysum plicatum subsp. pseudoplicatum capitulum on a human dermal fibroblast (HDF) cell line in this study. H plicatum subsp. pseudoplicatum capitulums were collected in Erzurum, Turkey (altitude 1950 m), dried, pulverized, and extracted with methanol. Firstly, total phenolic contents were determined and secondly, the proliferative effect, oxidative stress activities, and wound healing effects on HDF cells were evaluated by the cell proliferation kit (XTT) test, total antioxidant status (TAS), and total oxidant status (TOS) commercial kits, and the scratch experiment by taking microscopic images of the cells at 0, 12, 18, and 24 h, respectively. Total phenolic content was found to be 142.00 +/- 0.73 mg gallic acid equivalent per gram (GAE/g) extract. The capitulum extract has a proliferative effect at 0.5 to 10 mu g/mL concentrations according to the XTT test results. It was observed that TAS levels significantly increased in the plant extract at the concentration ranges 1 to 10 mu g/mL (P < .01). About 1 to 5 mu g/mL plant extract started to increase cell migration at the 12 h and significantly closed the wound area at the 24 h. At the doses between 1 to 5 mu g/mL, it has the most substantial effect on both cell viability and antioxidant effect, and wound healing was found to be in this concentration range. These findings suggested that the H plicatum subsp. pseudoplicatum capitulum is a valuable source of phenolic content with important antioxidant activity at wound healing and it was concluded that the capitulum extract accelerates wound healing by increasing cell migration in low doses.