Browsing by Author "Hassanein, Wael S."

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Electrospun Eugenol-Loaded Gelatin Nanofibers as Bioactive Packaging Materials To Preserve Quality Characteristics of Beef
(Elsevier, 2022) Yilmaz, Mustafa Tahsin; Hassanein, Wael S.; Alkabaa, Abdulaziz S.; Ceylan, Zafer
In this study, electrospinning technique was employed to fabricate eugenol loaded gelatin nanofibers (ELGNs) by using gelatin as an active material to encapsulate eugenol. The results indicated that eugenol could be suc-cessfully encapsulated within nanofibers, as verified by SEM, FTIR, and zeta potential analyses. The average zeta potential value of ELGNs was determined to be -15.08 mV. The average diameter of gelatin nanofibers was enlarged with eugenol encapsulation, increasing to diameter of 125 nm. ELGNs had a smooth and uniform fiber morphology observed by SEM. The ELGNs demonstrated a potent antibacterial activity by significant (P < 0.05) reduction in the number of total mesophilic aerobic bacteria counts by 1.62 log and psychrophilic aerobic bacteria counts by 1.33 log in fresh beef samples treated with ELGNs. Moreover, the bioactive ELGNs maintained textural properties (hardness-4.75 N, gumminess-1.75 N, and chewiness-3.87 N x mm at 9th day of storage) and sensory properties, exhibiting advantages as antimicrobial packaging materials that maintained the quality and freshness of the raw meat, along with the extension of its shelf life to 9 days. Significant (P < 0.05; 0.01) negative and positive correlations were found among the microbiological, textural, and sensory parameters, which explained and confirmed the reason for the effectiveness of the nanofiber treatment. In conclusion, our results suggested that the electrospinning approach in this study could be an effective technique to encapsulate eugenol within gelatin nanofibers and the electrospun ELGN mats could be a novel bio-degradable active packaging material with good antibacterial effects.
Use of Encapsulated Pomegranate Seed Oil in Novel Coarse and Nanosized Materials for Improving the Storage Life of Strawberry
(Elsevier Sci Ltd, 2024) Hassanein, Wael S.; Meral, Raciye; Ceylan, Zafer; Ahmed, Marwa M.; Yilmaz, Mustafa Tahsin
Different-sized pomegranate seed oil-based emulsions (coarse (CsP) and nanoemulsions (NsP): 1246 and 325 nm) were successfully prepared. Strawberries treated with NsP and CsP showed a significant decrease (p < 0.05) in yeast-mold counts (TMY) by 1.80 log CFU g(-1), and mesophilic aerobic bacteria counts (TMAB) decreased (p < 0.05) by 0.91 log CFU g(-1), respectively. CsP- and NsP-treated strawberries had a TPC of 74.45 and 82.35 mg GAE kg(-1), respectively, while control samples had a TPC of 44.24 mg GAE kg(-1). The strawberries treated with NsP exhibited the highest antioxidant capacity with 179.44 mol TEAC g(-1). After treatment with a coarse emulsion, severity levels of A. niger and B. cinerea were 60 and 73 % while the nanoemulsion treatment significantly reduced severity levels to 55.3 and 56 %. The coarse and nanoemulsions may have potential use within the food industry owing to their antioxidant and antifungal properties as well as their ability to enhance strawberry quality and function.