Browsing by Author "Huyut, Z."

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Actions of Resveratrol and Serotonin Added To Stored Bloods on Lipid Peroxidation and Oxidative Dna Damage
(Yuzuncu Yil Universitesi Tip Fakultesi, 2018) Huyut, Z.; Şekeroğlu, M.R.; Alp, H.H.; Balahoroğlu, R.; Çokluk, E.
Stored blood for use in diseases such as acute blood loss, injury and anemia can be maintained for about a month. It was reported that some negative changes called "storage lesions" occur in stored bloods time dependently. In order to delay these changes, preservatives are added to stored bloods. In this study, we aimed to determine the effects of resveratrol and serotonin on oxidative DNA and mitochondrial damage and lipid peroxidation in stored blood. Blood was taken from 10 volunteers to different blood bags and each blood was separated into 3 groups (control, resveratrol and serotonin groups). Malondialdehyde (MDA), ubiquinone-10 (CoQ10) and 8-hydroxy-2-deoxyguanosine/106deoxyguanosine (8-OHdG/106dG) levels were determined at baseline and on the 7th, 14th, 21st and 28th day after addition of resveratrol and serotonin. MDA, CoQ10 and 8-OHdG/106dG levels increased over time in the control and serotonin groups (p<0.05). However, resveratrol protected against the time-dependent increase in MDA and CoQ10 values (p>0.05). MDA and CoQ10 levels were significantly lower in the resveratrol-treated group on the 21st and 28th days compared with the other groups (p<0.05). These results show that oxidation sensitivity increased time dependently in stored blood. However, the addition of resveratrol allowed the extension of the blood’s shelf life by protecting against increased lipid peroxidation and oxidized CoQ10. © 2018, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Can the Irisin Be a Biomarker for Prostate Cancer? a Case Control Study
(Asian Pacific Organization for Cancer Prevention, 2020) Aslan, R.; Alp, H.H.; Eryılmaz, R.; Huyut, Z.; Sevim, M.; Araz, S.; Taken, K.
Aim: There is much evidence of an association between cancer and irisin that is an adipokine. This study researched on the relationship between prostate cancer (PCa) and irisin levels, and whether irisin can be used as a biomarker in the diagnosis of PCa. Materials and Methods: For the study groups, 50 primary PCa patients and 30 healthy male subjects were included in the PCa and healthy control groups, respectively. All volunteers in the healthy control group were screened for prostate cancer and other malignancies and chronic diseases. Volunteers who were determine to be completely healthy were included for healthy control group. In the serum samples of the subjects were measured free PSA, total PSA and irisin levels. Irisin levels were compared separately in terms of the Gleason scores and T stage. In addition to intergroup comparisons, the ROC curve for the irisin was plotted and power analysis was performed. Results: Free and total PSA levels in the PCa group were significantly higher compared to the healthy control group (p<0.05). In addition, irisin levels in the PCa group were significantly lower than in the healthy control group (p<0.05). There was no significant difference between irisin levels in the groups classified in terms of Gleason scores (p>0.05). When the cut-off value was taken as 8.1, the sensitivity and specificity of irisin for PCa were as 80.5% and 90%, respectively. Conclusion: The results of this study indicate that the levels of irisin in the PCa group are considerably reduced and irisin may be used as a biomarker as well as free and total PSA. © This work is licensed under a Creative Commons Attribution Non Commercial 4.0 International License.
Corrigendum To “effect of Ferritin, Inr, and D-Dimer Immunological Parameters Levels as Predictors of Covid-19 Mortality: a Strong Prediction With the Decision Trees” [Heliyon Volume 9, Issue 3, March 2023, Article E14015] (Heliyon (2023) 9(3), (S2405844023012227), (10.1016/J.heliyon.2023.e14015))
(Elsevier Ltd, 2025) Huyut, M.T.; Huyut, Z.
In the original published version of this article, the ethics committee approval number was missing in the Ethical approval section: Institutional Review Board Statement: The dataset used in this study was collected to be used in various studies in the estimation of the diagnosis, prognosis, and mortality of COVID-19. The necessary permissions for the collected dataset were given by the Ministry of Health of the Republic of Turkey and the Ethics Committee of Erzincan Binali Yıldırım University. This study was conducted following the 1989 Declaration of Helsinki. Erzincan Binali Yıldırım University Human Research Health and Sports Sciences Ethics Committee. This has now been added and the Ethics approval section revised to be more concise. The correct version can be found below: Institutional Review Board Statement: The dataset used in this study was collected to be used in various studies in the estimation of the diagnosis, prognosis, and mortality of COVID-19. The necessary permissions for the collected data set were obtained from the Ministry of Health of the Republic of Turkey. This study was approved by Erzincan Binali Yıldırım University Clinical Research Ethics Committee (31.03.2022, Report No: 03–09). The authors apologize for the errors. Both the HTML and PDF versions of the article have been updated to correct the errors. © 2024 The Author(s)
Do Avanafil and Zaprinast Change Some Selected Cytokine Levels in Ovariectomized Rat’s Liver
(Yuzuncu Yil Universitesi Tip Fakultesi, 2020) Huyut, Z.; Bakan, N.; Çokluk, E.; Akbay, H.İ.; Alp, H.H.; Şekeroğlu, M.R.
Studies reported that phosphodiesterase-5 inhibitors (PDE-5Is) positively contributed to bone-mineral-density and thickness in rats with ovariectomy, which have the same condition with postmenapozal period. To explain the positive contribution mechanism on bone mineral density of PDE-5Is, we investigated the effect of zaprinast and avanafil on levels of some pro-or anti-resorptive cytokines in ovariectomized-rats. Albino female rats (8 months and 250-350 g) were used and four groups of equal-number were randomly assigned (n=6). Groups; was the sahm operated, positive control (OVX), Zaprinast and OVX, Avanafil and OVX groups, respectively. The levels of Estrogen, IL-1β, IL-6, IL-8, IL-10 and TNF-α were measured by ELISA kits, in liver of rats. IL-1β, IL-6, IL-8 and TNF-α levels were high in groups with OVX compared to sham group, while IL-10 levels were low. Also, IL-1β, IL-6, IL-8 and TNF-α levels were low in zaprinast and especially avanafil-treated groups with OVX and were similar to the sahm group values (p=0.001 for IL-1β, p=0.045 for IL-6, p=0.008 for IL-8, p=0.006 for IL-10, p=0.026 for TNF-α). Zaprinast and especially avanafil inhibited IL-1β, 8 and TNF-α and increased the IL-10 levels compared to the OVX group. This may support opinion that PDE-5Is enhance bone mineralization by inhibiting proresorptive cytokines. © 2020, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Effect of Intermittent Fasting During Acrylamide Exposure on Blood Copper, Zinc and Lead Levels in Rats
(Yuzuncu Yil Universitesi Tip Fakultesi, 2018) Huyut, Z.; Arihan, O.
Zinc and copper are responsible for certain physiological functions in the body. On the other hand lead is a toxic trace element. In this study, effect of intermittent fasting on blood zinc, copper and lead levels during acrylamide exposure was studied. Forty female adult Wistar albino rats were divided into 4 groups (n=10 in each group). First group: control group without any administration, second group: acrylamide group in which 20 mg/kg/day acrylamide was administered for 30 days, third group: intermittent fasting group in which 12 hours of fasting were administered overnight for 30 days, fourth group: intermittent fasting and acrylamide group in which 20 mg/kg/day acrylamide and 12 hours of intermittent fasting was administered for 30 days. At the last day of experimental procedure blood was withdrawn under anesthesia. Zinc as well as copper levels were measured from plasma samples in flame unit and lead was measured from erythrocyte packages in combustion unit of Atomic Absorption Spectrophotometer. In acrylamide group, zinc was lower (p<0.05) whereas copper (p>0.05) and lead levels (p<0.05) were increased. In intermittent fasting group zinc level was higher whereas copper and lead levels were lower compared to acrylamide group (p<0.05). In acrylamide+intermittent fasting group lead level was significantly lower (p<0.05). Conclusion: Results indicate an augmentation of toxic copper and lead elements due to acrylamide administration whereas a decrease in zinc which is an important element for growth and immune system. On the contrary, intermittent fasting decreased such untoward effect of acrylamide and increased potential detoxification of toxic chemicals. © 2018, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
The Effect of Pde5 Inhibitors on Bone and Oxidative Damage in Ovariectomy-Induced Osteoporosis
(Wiley-blackwell, 2016) Alp, H. H.; Huyut, Z.; Yildirim, S.; Basbugan, Y.; Ediz, L.; Sekeroglu, M. R.
Effect of Selenium and N-(p Anthranilic Acid on Doxorubicin-Induced Kidney Injury in Rats
(Inonu University, 2023) Yildizhan, K.; Huyut, Z.; Altindağ, F.; Uçar, B.
Doxorubicin (DOXR) is one of the essential antitumor drugs. However, its serious adverse effects in many organs limit the clinical use of DOXR. This study aimed to investigate the effect of selenium (Se) and N-(p-Amilcinnamoyl) anthranilic acid (ACA) on kidney tissue in DOXR-administered rats. The rats in the study were divided into six groups (n=10); Control, DMSO, DOXR, DOXR+Se, DOXR+ACA and DOXR+Se+ACA. At the end of the study, intracardiac blood was drawn from rats, and kidney tissues were removed. Urea and creatine levels were measured in serum samples of rats. In addition, histopathological examination of kidney tissue was determined by H&E staining, and 8-OHdG expression was determined by immunohistochemical analysis. Urea and creatine levels increased with DOXR decreased in serum samples after Se and ACA treatments (p< 0.05). While glomerular atrophy, tubular and glomerular dilatation, vascular occlusion and degeneration of tubular epithelial cells were observed in the DOXR group, significant improvement was observed in the Se and ACA treatment groups. In addition, Se and ACA treatments reduced DOXR-induced 8-OHdG expression (p< 0.05). These findings indicated that Se and ACA could be used as critical therapeutic agents to suppress renal dysfunction and oxidative DNA damage that can occur after DOXR-induced kidney injury. © 2023, Inonu University. All rights reserved.
Effect of Urtica Dioica Seed Extract on Aquaporin 1 and 7, Caspase-3 and Oxidant/Antioxidant Status in Diethylnitrosamine-Induced Kidney Damage in Rats
(Yuzuncu Yil Universitesi Tip Fakultesi, 2024) Keleş, Ö.F.; Huyut, Z.; Yıldızhan, K.; Demir, A.
Diethylnitrosamine (DENA) is known to have a carcinogenic effect on the liver by stimulating oxid ative stress and inflammation. There are few studies on the negative effects of DENA on kidney tissue, and the therapeutic effect of Urtica dioica Seed Extract (UDSE) against the negative effects of DENA is investigated for the first time in this study. Th is study investigated the protective effect of UDSE on kidney tissues of rats administered DENA. The rats were divided into four groups (n=8): control, UDSE (1 mL/kg/daily for 16 weeks), DENA (200 mg/kg/first day), DENA+UDSE (200 mg/kg/first-day single dose, and 1 mL/kg/daily for 16 weeks). At the end of the experiment, blood and kidney tissues were taken for biochemical and pathological analysis. DENA administration has been shown to increase oxidative stress in the kidneys and reduce antioxidant levels. Moreover, compared to the DENA group, DENA decreased the level of aquaporin (AQP)-1 in kidney tissue, while UDSE treatment increased both AQP-1 levels in kidney tissue and AQP-1 and 7 levels in the serum samples. Histopathological examination of the kidney revealed significant coagulation necrosis, especially in proximal tubular epithelial cells, hyperemia in capillaries, mononuclear cell infiltration between tubular area s, atrophy in the glomerular cluster, and adhesions with Bowman's capsule. In addition, there was a decrease in both caspase-3 (immunohistochemically) expression and TOS levels in the DENA+UDSE group compared to the DENA group, while an increase was seen in antioxidant levels. These findings indicated that UDSE may be an essential therapeutic agent against DENA-induced kidney injury. © 2024, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Effects of Chronically Exposure To Flor and 7,12-Dimethylbenzanthracene (Dmba) on Spermatogenesis and Testicular Histopathology in Rats
(Ataturk Universitesi, 2018) Yildirim, S.; Belhan, S.; Uyar, H.; Huyut, Z.; Oto, G.; Sağlam, Y.S.
This study aimed to investigate the effects of both sodium fluoride (NaF) and polycyclic aromatic hydrocarbon (PAH) derivative such as 7,12-dimethylbenz[a]anthracene (DMBA) singly and in combination on spermatogenesis and testis histopathology in rats. Forty male Wistar albino rats weighing 250–300 g used. On the 90th day of the experiment, intracardiac blood samples and testes were taken. While there was no change in FSH and LH levels in the groups, testosterone levels were significantly decreased in the other groups according to the control group (P<0.001). It was decreased the ratio of motility of sperm, sperm density and, increased the abnormal sperm rate by chronic fluorosis and DMBA toxicity in rats significantly. In histopathological examinations; A decrease in the number of spermatocytes and spermatozoa in the tubulus wall of the testes of rats in the NaF (30 ppm) group was observed. When testicular tissues of rats in the DMBA (10 mg/kg) group were examined, atrophy in tubulus, edema in intertubular spaces and a decrease in the number of spermatocytes in the tubulus wall were found. When testes tissues of rats in the group of NaF (30 ppm) + DMBA (10 mg/kg) were examined, atrophy in seminiferous tubules, severe degenerative and necrotic changes in Spermatogoniums on the walls of tubules and thinning in tubule walls were observed. There is not found spermatozoon in the tubul lumens. Severe edema in the intertubular spaces, dilatation and hyperemia in the veins found in these regions. As a result, it was observed that NaF and DMBA had a negative effect on testicular tissue and fertility singly, and in combination, this effect was further exacerbated. © 2018 Ataturk Universitesi.All Rights Reserved.
The Effects of Curcumin Nanoparticles on an Experimental Diabetes Model: Reproductive Hormones, Lipid Profile and Immunohistochemical and Immunofluorescence Markers
(B W K Publishing Solutions & verlag, 2024) Belhan, S.; Huyut, Z.; Yildirim, S.; Algul, S.
Diabetes affects many organs and causes serious problems for reproductive health. There is still no effective treatment but a range of new strategies are being used to treat and to reduce the possible complications of diabetes. One of the most promising of them relies on the use of nanoparticles. We have evaluated the effects of curcumin and curcumin nanoparticles on blood glucose, lipid profile, reproductive hormones and histopathological, immunohistochemical and immunofluorescent parameters in experimental diabetic conditions. We created eight study groups (control group, diabetes group, curcumin group, Mg-curcumin nanoparticle group, diabetes+curcumin group, diabetes+Mg-curcumin nano- particle group, encapsulated Mg-curcumin nanoparticle group, diabetes+encapsulated Mg-curcumin nano- particle group), allocating rats to the groups at random. Triglyceride, cholesterol and LDL levels were high in the diabetes group, NOP10 and PI3K levels were intense and reproductive hormone levels were low. Curcumin and curcumin nanoformulations administered to the diabetes groups led to significant improvements in these parameters. The testosterone level, which was low in the diabetes group, increased significantly after the application of encapsulated Mg-curcumin. Curcumin nanoformulations were more effective than curcumin at countering the diabetes-impaired lipid profile and improving the levels of reproductive hormones, the histopathological findings and PI3K and NOP10 expression.
Essential Trace Element Levels in Patients With Cutaneous Anthrax
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Binici, I.; Parlak, M.; Sunnetcioglu, M.; Baran, A.I.; Ceylan, M.R.; Kul, A.R.; Huyut, Z.
The causative microorganism in anthrax is Bacillus anthracis and this disease is more common in some regions of Türkiye. Changes in trace elements other than iron in anthrax infection have not been studied. In this study, iron, copper, lead, zi nc, manganese, magnesium, cadmium and cobalt levels were investigated in cases with cutaneous anthrax. Fifteen patients with cutaneous anthrax and 15 healthy individuals wereincluded in the study. The groups were similar to each other in terms of age and gender. Anthrax was diagnosed according to contact status with animals, symptoms, examination, and microbiological results. We performed our study with Atomic Absorption Spectrophotometer (UNICAM-929 spectrophotometer). Serum iron, lead, and cadmium levels were significantly higher in the patients than in the control subjects (p<0.05). Serum magnesium, manganese, zinc, copper, and cobalt levels were significantly lower in the patients than in the control subjects (p<0.05). The copper/zinc molar ratio was not significantly increased in the patients with cutaneous anthrax than in the control subjects. It has been reported that iron, cadmium, and lead levels are low and copper level is high in infectious diseases. But we determined the opposite situation in the patients with cutaneous anthrax. As a result, it can be said that the detection of high lead and cadmium levels in the cell in anthrax disease suppresses the immune system. Also, zinc can be used as a marker for this disease. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Hepatoprotective Effect of Foeniculum Vulgare Essential Oil: a Carbon-Tetrachloride Induced Liver Fibrosis Model in Rats
(2004) Öbek, H.; Uǧraş, S.; Bayram, I.; Uygan, I.; Erdoǧan, E.; Öztürk, A.; Huyut, Z.
Hepatoprotective activity of Foeniculum vulgare (fennel) essential oil was studied using a carbon tetrachloride-induced liver fibrosis model in rats. The hepatotoxicity produced by chronic carbon tetrachloride administration was found to be inhibited by Foeniculum vulgare essential oil with evidence of decreased levels of serum aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase and bilirubin. Histopathological findings also suggest that Foeniculum vulgare essential oil prevents the development of chronic liver damage. The changes in body weights in the rats assigned to the study groups supported these biochemical and histopathological findings. The results of this study clearly indicate that Foeniculum vulgare essential oil has a potent hepatoprotective action against carbon tetrachloride-induced liver fibrosis in rats.
In Stored Human Blood, the Inhibitor Effect of Tannic Acid and Caffeic Acid on Lipid Peroxidation and Oxidative Dna Damage
(Yuzuncu Yil Universitesi Tip Fakultesi, 2016) Huyut, Z.; Şekeroğlu, M.R.; Balahoroğlu, R.; Alp, H.H.; Çokluk, E.
Stored bloods, are exposed to a number of negative changes called as "storage lesions" morphological, biochemical and functional, to begin the first phase of the storage. In this study, we investigated the effects of caffeic and tannic acid in lipid peroxidation and oxidative DNA damage in stored blood. The blood was taken from 10 healthy male individuals to the blood bag containing CPD. Each donor's blood was separated into three groups. The first group was used as the control group with nothing added to the blood. A total of 30 µg/mL caffeic acid and 15 µg/mL tannic acid was added to the second and third group, respectively. Malondialdehyde, ubiquinone-10 and 8-hydroxy-2 deoxyguanosine levels were determined at baseline and on the 7th, 14th, 21st and 28th day, by high pressure liquid chromatography. The MDA and 8-OHdG levels of control and tannic acid groups were increased on the 21st and 28th days (p<0.001), whereas in caffeic acid group was preserved. Also, CoQ10 levels increased in the control group time dependently (p<0.001), but preserved in the other two groups. In addition, CoQ10 and 8-OHdG levels of tannic and especially caffeic acid groups was lower than the control group in the inter-group comparison, in periods of progressing time (p<0.05). These results showed that oxidative stress increased in the stored blood, but adding tannic and caffeic acid into the stored blood can attenuate the effects of oxidation. © 2016, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Investigation of Histopathological and Biochemical Changes in Rats Feeding Roasted and Raw Sunflower (Helianthus Annuus L.) Seed Extract
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Sinan, M.T.; Huyut, Z.; Yıldızhan, K.; Keleş, Ö.F.; Yener, Z.
Today, sunflower seeds, which are in first place in the consumption of nuts, have many benefits. However, sunflower seeds are consumed mainly by roasting. In this study, the effect of roasted and raw sunflower seeds on the liver of rats fed with experimentally roasted and raw sunflower seeds was investigated histopathologically and biochemically. For this purpose, a total of 24 rats were with eight rats in each group; the control, roasted sunflower seed (ROSS), and raw sunflower seed (RASS) groups. At the end of the experiment, blood and tissue samples were taken from the rats for histopathological and biochemical examinations. While degenerative-necrotic changes were detected in the livers of rats fed with roasted sunflower seeds, no significant morphological changes were detect ed in the raw sunflower and control groups. Furthermore, there was no significant difference between the groups in the biochemi cal parameters AST, ALT, CAT, GSH, BcL-2 and SOD. However, TAS levels in the ROSS group were significantly lower than in the other groups, while LDH, Bax and caspase-3 levels were high. Data showed that ROSS decreased antioxidant status in liver tissue and increased LDH levels compared to RASS and could damage liver tissue. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Investigation of the Predictive Value of Amino Acids for Tuberculous Meningitis, Aseptic Meningitis and Bacterial Meningitis
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Binici, I.; Huyut, Z.; Alp, H.H.; Akbay, H.I.; Karsen, H.; Parlak, M.; Akmeşe, Ş.
In our study, we aimed to detect amino acid changes, if any, by comparing the levels of amino acids in cerebrospinal fluid (CSF) samples of patients with aseptic, bacterial, and tuberculous meningitis and control groups. Patients diagnosed with aseptic meningitis (n=41), tuberculous meningitis (n=21), bacterial meningitis (n=41) and a control group consisting of 64 individuals with similar gender and age characteristics were included in the study. 2 mL of cerebrospinal fluid specimens were obtained from all patients and control group and sto red at-80 °C until the study day. The amino acid measurements were performed using commercially available liquid chromatography-tandem mass spectrometry method (LC-MS / MS) kits. When we investigated amino acids levels in all groups, the levels of 1-methyl histidine, alanine, asparagine, histidine, isoleucine, lysine, methionine, norvaline, ornithine, phenylalanine, proline, sarcosine, threonine, tyrosine, valine in the aseptic, tuberculosis and bacterial meningitis groups were statistically higher than t hose of the control group. The levels of all amino acids except cystine, glutamic acid, homo-citrulline, and taurine in the patients with aseptic meningitis were statistically significantly higher than in the control group. The levels of all amino acids ex cept homocitrulline, and tryptophan were statistically significantly higher in the patient group with tuberculous meningitis than in the control group. The levels of all amino acids except glutamic acid were statistically significantly higher in the patient group with bacterial meningitis than in the control group. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Levels of Leukocyte Oxidative Dna Damage (8-Ohdg), Serum Coenzyme Q10 and Lipid Peroxidation in the Formation Attacks of Patients With Multiple Sclerosis
(Suleyman Demirel University, 2017) Cokluk, E.; Milanlıoğlu, A.; Huyut, Z.; Çilingir, V.; Alp, H.H.; Aydın, M.N.; Balahoroğlu, R.
Multiple sclerosis (MS) is a demyelinating disease of the nervous system. Evidence about oxidative stress plays an important role in the pathogenesis of MS is increasing day by day. In our study, we aimed to investigate the effect of oxidative DNA damage and oxidative stress in the pathogenesis of MS disease. Blood samples were obtained from during an attack (Group 1), between attacks (Group 2) of MS patients (20 male and 10 female) and 30 healthy volunteers (Group 3). Malondialdehyde (MDA) levels as indicator of oxidized lipids were detected using flourescence dedector with high pressure liquid chromatograph (HPLC). DNA was extracted from leukocytes of control and patients with MS and then we measured 8-hydroxy-2' -deoxyguanosine (8-OHdG) and deoxyguanosin (dG) by using HPLC method with electrochemical and UV detector, respectively. Measurement of oxidized coenzyme Q10 (CoQ10) and reduced CoQ (CoQ10H) was performed by using UV detector with HPLC method. Serum MDA level of group 1 was significantly higher than those in group 2 and group 3 (p< 0.001). 8-OHdG/106 dG ratio of group 1 was significantly higher than those in group 2 and group 3 (p< 0.001). CoQ10/CoQ10H rates of group 1 were significantly increased compared with group 2 and group 3 (p<0.001). In conclusion, we observed that oxidative DNA damage, lipid and mitochondria oxidative damage were high in blood of patients with MS. It seems that oxidative stress acts a play role the pathogenesis of MS patients as well as induces attacks. © 2017 Suleyman Demirel University. All rights reserved.
Neuroprotective Effects of Ghrelin in Cuprizone-Induced Rat Model of Multiple Sclerosis
(Springer, 2025) Oruk, S.; Ergul Erkec, O.; Huyut, Z.; Acikgoz, E.
Multiple sclerosis (MS) is an inflammatory central nervous system disease characterized by demyelination and axonal loss and is the main cause of non-traumatic neurological disability in young adults. Although there are several treatment approaches to manage the disease, there is no definitive cure for multiple sclerosis. Inflammation and oxidative stress are known to play important roles in the pathophysiology of MS. Ghrelin, a peptide secreted by the stomach, is reported to have neuroprotective properties through several pathways, including attenuating oxidative stress and inflammation. In the present study cuprizone (CPZ)-induced model of MS was used in Wistar albino rats to study the possible anti-inflammatory, antioxidant and neuroprotective effects of ghrelin. Rats were randomly divided into six groups: Control groups (Control35 and Control-S42), demyelination group, remyelination group, remyelination + ghrelin (20 µg/kg) group and remyelination + ghrelin (40 µg/kg) group. Y maze test was performed on the rats on their last day of the experiment. Oxidative stress and inflammatory parameters were investigated in brain using commercial kits by enzyme-linked immunosorbent assay (ELISA). Luxol fast blue (LFB) and hematoxylen&eosin (H&E) staining were performed in brain tissues. CPZ leads to a significant decrease in glutathione peroxidase (GSH-Px) levels and myelin content and a significant increase in malondialdehyde (MDA), tumor necrosis factor-alpha (TNF-ɑ), interleukin- 6 (IL- 6) levels, the number of lymphatic cells and inflammatory cells. A significant increase in the antioxidant parameter levels and a significant decrease in MDA levels were found in the ghrelin treated groups (p < 0.05). CPZ leads to irregular, fragmented, demyelinating nerve fibers. A more significant remyelination was observed in the ghrelin treated groups compared to the other groups (p < 0.05). In conclusion, ghrelin treatment showed neuroprotective and antioxidant properties and reduced demyelination in the CPZ-induced rat model of multiple sclerosis. © The Author(s) 2025.
A Novel Indicator for Erectile Dysfunction: S100a4
(Yuzuncu Yil Universitesi Tip Fakultesi, 2024) Demir, M.; Huyut, Z.; Huyut, M.T.; Ertaş, K.; Aslan, R.; Eryılmaz, R.; Taken, K.
This study aimed whether S100A4 would be useful in predicting Erectile Dysfunction (ED) and ED severity. This prospective study included 88 male volunteers aged 18-80 years. The control group consisted of 44 healthy patients and the diabetic group consisted of 44 patients with type-2 diabetes (T2DM). Age, body mass index, smoking status, hypertension status, International Index of Erectile Function 1-5 scores of all the volunteers were evaluated, and also glucose, HbA1c, cholesterol, testosterone, prolactin and S100A4 levels were measured in the serum samples. The relationships between S100A4 and erectile functions were investigated with appropriate statistical analyzes. The mean age of the T2DM group was 51.98 ±10.91 years, while the control group’s mean age was 53.77 ±12.46 years and there was no significant difference between them (p=0.31). Glucose, HbA1c, mean ED severity and S100A4 levels in the T2DM group were higher than in the control group, while testosterone level was lower than in the control (p≤0.05). In addition, it was found that S100A4 levels increased due to the increase in ED severity in both groups (p<0.001). In addition to ED severity, smoking and hypertension were seen as the factors that most affected S100A4. The results indicated that S100A4 may be a useful biomarker in determining ED and ED severity. © 2024, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Oxidative Stress in Rats During Exposure To Cigarette Smoke and After Smoking Cessation
(Wiley-blackwell, 2016) Donder, A.; Balahoroglu, R.; Huyut, Z.; Yildirim, S.; Dulger, H.; Ilhan, F.; Yilmaz, A.
The Susceptibility of Autoxidation of Erythrocytes During Storage of Blood: Effects of Melatonin and Propofol
(Amer Assoc Clinical Chemistry, 2008) Sekeroglu, M. R.; Huyut, Z.; Dulger, H. H.; Dilek, I.