Browsing by Author "Huyut, Zuebeyir"

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Bruxism and Stress: Ultrasonographic Masseter Muscle Assessment and Salivary Melatonin-Cortisol Dynamics
(Wiley, 2025) Kaya, Sema; Koc, Alaettin; Huyut, Zuebeyir
BackgroundBruxism is a parafunctional activity characterised by teeth grinding or clenching, often associated with stress and neurophysiological factors. Its aetiology is multifactorial, with recent studies emphasising neurotransmitters and hormonal imbalances, particularly melatonin and cortisol. This study assesses masseter muscle thickness using ultrasonography and examines the relationship between salivary melatonin and cortisol levels in bruxism. ObjectiveThis study aimed to evaluate the ultrasonographic characteristics of the masseter muscle in individuals with bruxism and investigate the association between salivary melatonin and cortisol levels. MethodsThis cross-sectional study included 80 participants (38 bruxists, 42 controls), aged 20-25 years. Bruxism was diagnosed through clinical examination and self-reported questionnaires. Masseter muscle thickness was measured via ultrasonography in relaxed and contracted states. Salivary melatonin and cortisol levels were analysed using enzyme-linked immunosorbent assay (ELISA). Group comparisons were conducted using independent t-tests and ANOVA, with correlation analyses using Pearson's tests. ResultsSalivary melatonin levels showed no significant difference between groups (p = 0.067), while salivary cortisol levels were significantly higher in the bruxism group (p = 0.001). No significant differences in masseter muscle thickness were observed in either state (p > 0.05). A weak but significant positive correlation existed between melatonin and cortisol levels (p < 0.05). ConclusionAlthough not statistically significant, melatonin levels tended to be higher in bruxist patients, possibly due to a negative feedback mechanism to prevent the side effects of cortisol such as oxidative stress or a balancing process of these hormones by cytokines involved such as IL-1 beta.
Effect of Abemaciclib and Curcumin Administration on Sex Hormones, Reproductive Functions, and Oxidative Dna Expression in Rats
(Taylor & Francis Ltd, 2024) Huyut, Zuebeyir; Ucar, Bunyamin; Yildizhan, Kenan; Altindag, Fikret; Huyut, Mehmet Tahir
This study investigated whether abemaciclib (ABE) administration had any adverse effects on ovarian and sex hormones in female rats, and the protective effect of curcumin. Forty female rats were equally divided into the sham control, DMSO, curcumin (CMN), ABE, and ABE+CMN groups. Pharmaceuticals were administered by gavage daily for 28 days. Serum sex hormones were measured in an autoanalyzer operating with a microparticle immunoassay method. In addition, histopathological examination and 8-OHdG expression were performed on the ovarian tissue. Progesterone and testosterone levels were significantly decreased, while estradiol levels were significantly increased, in the ABE group compared to the sham and DMSO groups. In addition, there were significant differences in sex hormone levels in the CMN and/or CMN+ABE groups compared to the ABE group. There was decreased expression of 8-OHdG in the ABE+CMN group compared to the ABE or CMN only groups. This study exhibited that ABE administration can adversely affect functions and histology of the ovarian tissue, but CMN therapy may be protective against the adverse effects on ovarian in ABE-induced rats.
Effect of Hesperidin on Lipid Profile, Inflammation and Apoptosis in Experimental Diabetes
(Maik Nauka/interperiodica/springer, 2025) Yildizhan, Kenan; Bayir, Mehmet Hafit; Huyut, Zuebeyir; Altindag, Fikret
In recent years, therapeutic approaches against diabetes-induced liver damage have attracted great interest. Studies indicate the anticarcinogenic, anti-inflammatory, antioxidant, and lipid-lowering potential of hesperidin (HESP), a flavonoid in citrus fruits. This study examined how HESP prevented streptozotocin (STZ)-induced diabetic liver damage. Four groups of seven rats each were created: Control, HESP (100 mg/kg/day), STZ (45 mg/kg), and STZ + HESP (45 mg/kg and 100 mg/kg/day, respectively). Serum AST, ALT, LDH, LDL, triglyceride, total cholesterol levels, and the TNF-alpha, IL-1 beta, and caspase-3 expression levels of liver tissue in the STZ group were higher than the other groups (p < 0.05). However, these values were significantly lower (p < 0.05) in the STZ + HESP group compared to the STZ group. Furthermore, administering HESP together with STZ reduced liver expression levels of caspase-3, TNF-alpha, and IL-1 beta, as well as blood levels of AST, ALT, LDH, LDL, triglyceride, and total cholesterol. HESP against diabetes-induced hepatic damage reduced proinflammatory cytokine levels, and returned the lipid profile, and apoptotic indicators to normal levels. These findings suggested that HESP therapy may be an important therapeutic role against diabetes-induced liver damage.
Effect of Selenium Against Doxorubicin-Induced Oxidative Stress, Inflammation, and Apoptosis in the Brain of Rats: Role of Trpm2 Channel
(Natl inst Science Communication-niscair, 2023) Yildizhan, Kenan; Huyut, Zuebeyir; Altindag, Fikret; Ahlatci, Adem
Doxorubicin (DOX) is widely used as an anticancer drug in humans' various solid and haematological tumours. Although many studies on the toxic effect of DOX are used in different organs, its impact on brain tissue has yet to be adequately studied. This study investigated the protective effect of selenium (Se) and the role of transient receptor potential melastatin-2 (TRPM2) channel activation against brain damage caused by DOX administration. Sixty rats were randomly divided into the sham, dimethyl sulfoxide (DMSO), DOX, DOX + Se, DOX + N-(p-amylcinnamoyl) anthranilic acid (ACA), and DOX + Se + ACA groups. The reactive oxygen species (ROS), poly [ADP-ribose] polymerase 1 (PARP1), and TRPM2 channel levels in brain tissues were measured by ELISA. In addition, a histopathological examination was performed in the cerebral cortex and hippocampal areas, and the TRPM2 channel, NF-icB, and caspase-3 expression were determined immunohistochemically. The levels of ROS, PARP1 and TRPM2 channel in the DOX group were higher than in the sham and DMSO groups (P < 0.05). However, these parameters were decreased in the in DOX+Se and DOX+ACA groups by the treatments of Se and ACA (P < 0.05). Also, we determined that Se and ACA treatment decreased the NF-icB, caspase-3, and TRPM2 channel expression in the cerebral cortex and hippocampal areas in the DOX-induced rats. The data showed that Se and/or ACA administration together with DOX administration could be used as a protective agent against DOX-induced brain damage.
The Effects of Berberine and Curcumin on Cardiac, Lipid Profile and Fibrosis Markers in Cyclophosphamide-Induced Cardiac Damage: The Role of the Trpm2 Channel
(Wiley, 2024) Huyut, Zuebeyir; Yildizhan, Kenan; Altindag, Fikret
Cyclophosphamide (CYP) is widely used to treat various types of cancer. In addition to the therapeutic properties of this drug, unfortunately, its side effects are still not fully understood. This study investigated the protective effect of curcumin (CURC) and berberine (BER) on CYP-induced cardiac damage. Thirty-six male rats were equally divided into the control, dimethyl sulfoxide (DMSO), CYP, CYP + CURC, CYP + BER and CYP + BER + CURC groups. Troponin-I, Creatine kinase-myocardial band (CK-MB), total cholesterol, triglyceride levels in serum samples, and reactive oxygen species (ROS), poly(ADP-ribose) polymerase-1 (PARP-1), and transient receptor potential melastatin 2 (TRPM2) channel levels in heart tissue were measured using an enzyme-linked immunoassay (ELISA) kit. In addition, histopathological examination and immunohistochemical investigation of the TRPM2 channel, fibroblast specific protein-1 (FSP1), transforming growth factor-beta- 1 (TGF-beta 1) and alpha-smooth muscle actin (alpha-SMA) expressions were determined in heart tissue. The CYP group's troponin-I, total cholesterol, triglyceride, CK-MB, ROS, PARP-1 and TRPM2 channel levels were higher than in the other groups in the ELISA measurements (p < 0.05). In contrast, these parameters in the group treated with CURC and BER together with CYP were lower than in the CYP group (p < 0.05). Additionally, CUR and BER reduced CYP-induced pathological damage, TRPM2, FSP1, TGF-beta 1 and alpha-SMA expressions. The data showed that CYP administration can cause cardiac damage by increasing the TRPM2 channel, TGF-beta 1, FSP1 and alpha-SMA expression levels. Therefore, we concluded that CURC and BER administration following CYP application may be used as therapeutic agents to prevent CYP-induced cardiac damage.