Browsing by Author "Ibrahim, A.S."

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Determination of Genetic Relations Among Tomato Accessions in Sulaymaniyah Region Through Issrs Markers Genetic Relations in Tomato Accessions
(Centenary University, 2020) Ibrahim, A.S.; Erdinc, C.
The goal of this study was to examine the genetic difference among thirty two tomato accessions (Solanum lycopersicum L.) grown in Sulaymaniyah using ISSR molecular markers technique. The results of ISSR markers revealed 65 amplified fragments, 50 of them were polymorphic from using 15 primers. Fifteen ISSR markers used to detect DNA polymorphism gave polymorphism percentage for each primer range between 50–100% with an average polymorphism percentage reaching 75.61%. ISSR6 and Sola 11 gave the highest polymorphism percentage was 100%, while 3F, Sola 5 and Sola 12 did not give any amplification. The mean of PIC (Polymorphic Information Content) value was 0.50 for ISSR markers. The similarity matrix was obtained by using Jaccard’s coefficients, based on polymorphic bands and dendrogram constituted through UPGMA cluster analysis. The dendrogram revealed 4 main groups. Genetic similarity (GS) ranged from 0.261 to 0.941 within studied accessions. The highest similarity was 0.941 for the genotype pairs G7 and G16. However, the lowest similarity index was 0.261 among G15-G24 and G15-G26. Tomato accessions were determined by two populations according to STRUCTURE analysis. The ISSR marker system ensured useful information in describing genetic diversity among tomato accessions collected from different geographical around Sulaymaniyah province. © 2020, Centenary University. All rights reserved.
Screening of Bean Genotypes Against Bean Common Mosaic Virus (Bcmv) by Artificial Inoculation and Molecular Confirmation
(Centenary University, 2023) Ibrahim, A.S.; Usta, M.; Şensoy, S.
Bean mosaic virus (BCMV) is a widespread plant pathogen that causes significant bean yield losses in several bean-growing regions worldwide. The use of resistant common bean varieties to BCMV is considered the most efficient and feasible approach to control its effects. Numerous genes and molecular markers associated with resistance to these pathogens have been discovered and used extensively in breeding studies around the world. Screening bean genotypes for resistance to these viruses is a critical step in developing resistant varieties. The goals of the study are to identify virus sources in the region and artificially inoculate Lake Van basin bean genotypes with BCMV. The recovered BCMV strain NL-4 was inoculated with 45 bean cultivars, most of which originated from the Lake Van basin in Turkey. Differentiation between resistant and susceptible was based on visual symptoms, and of the 45 genotypes, 29 were found to be resistant to NL-4, while 16 genotypes were susceptible (8 of them moderately susceptible and 8 of them highly susceptible). © 2023, Centenary University. All rights reserved.