Browsing by Author "Icen, Hasan"

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Comparison of Classic, Theranekron and Classic-Plus Theranekron Treatment on the Foot and Mouth Disease Lesions in Cattle in Van, Diyarbakir and Ankara Regions in Turkey
(Medwell online, 2012) Duz, Erkan; Icen, Hasan; Arserrim, Neval B.; Cakmak, Firat; Bakir, Bahtiyar; Uysal, Ersin
The study compared the influence of Tarantula cubensis extract 1:100/D2 (Theranekron (R), Richter Pharma, Austria) and classic treatment (daily injection of fluniksin meglumin., oxytetracycline, washing the mucosa and feet lesions with 10% sodium bicarbonate and antibiotic spray for 3 consecutive days) for the oral, hoof and mammary lesions treatment in foot and mouth disease in cattle. Ninety mixed breed cattle with foot and mouth diseases were used. Clinical symptoms included fever inappetance oral, hoof and mammary lesions. The cattle were divided into three groups. The first group, the second and the third were treated with classic, theranekron and theranekron plus classic treatments respectively. About 48 h after theranekron and theranekron plus classical application, recovery occurred and body temperature, appetence returned to normal more rapidly than that of the group on which only classical treatment methods were used.
Comparison of Tuberculin Skin Test, Ifn-Γ Assay, Real Time Pcr and Lateral Flow Rapid Test in Diagnosis of Field Outbreaks of Bovine Tuberculosis
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Kaya, Abdullah; Icen, Hasan; Tuzcu, Nevin; Simsek, Aynur; Yesilmen, Simten; Kochan, Akin; Alan, Adil
Bovine tuberculosis is an important zoonotic disease transmitted by direct contact, respiratory pathway, ingestion of unpasteurised milk and milk product, raw or undercooked meat. Tuberculosis can be difficult to diagnose based only on the clinical signs. Tuberculosis is usually diagnosed in the field with the tuberculin skin test. Sputum and other body fluids may be collected for microbiological examination. Polymerase chain reaction (PCR) methods have also been described. Diagnostic blood tests include the lymphocyte proliferation assay, the interferon gamma (IFN-gamma) assay, and enzyme-linked immunosorbent assays (ELISA). In this study a total of 50 animals were tested by using tuberculin skin test (TST), lateral flow rapid test, IFN-gamma assay and real time PCR. The animals were selected randomly among 178 cattle in dairy farms with the aged between 3-5 years and suspected of having tuberculosis. Forty five cattle were positive out of 50 for TST while 31 for reactive by the IFN-gamma assay and 28 for rapid test and 9 for real time PCR. The purpose behind such variable as age was to compare sensitivity of tuberculin skin test, the IFN-gamma assay and TB lateral flow rapid test and real time PCR examination for the diagnosis of field outbreaks of bovine tuberculosis in Turkey.
Diaphragmatic Hernia in a Sheep - a Case Report
(Univ Zagreb vet Faculty, 2018) Simsek, Aynur; Yaman, Turan; Icen, Hasan; Kochan, Akin
A two-year-old sheep was referred to the clinics of the Department of Internal Medicine of Dicle University, Faculty of Veterinary Medicine, with signs of anorexia, abdominal tympany and constipation. The clinical examination of the animal revealed the presence of respiratory failure associated with weaker lung sounds on the right side of the body when compared to the left side. There were no ruminal movements, and when the rumen was probed with a stomach tube, no ruminal content was obtained. The animal was euthanized upon the request of the owner, and a necropsy was performed. At necropsy, it was observed that the left hepatic lobe had protruded into the thoracic cavity through a defect in the diaphragm, and that a diaphragmatic hernia had developed.
Neonatal Buzağılarda Lactoferrinin İshalde Koruyucu ve Terapotik Etkisinin Araştırılması
(2024) Işık, Nurettin; Kaya, Abdullah; Icen, Hasan
Bu çalışma Van ve Diyarbakır illerinde neonatal ishalli buzağılarda rotavirus, coronavirus, Cryptosporidium, E. coli F5 (K99), Cl. perfringens ve Salmonella spp.’nin prevalansı ile klinik, hematolojik, biyokimyasal değişiklikler ve standart tedavide laktoferrin ilavesinin etkinliğini araştırmak amacıyla yapıldı. İshalli buzağılarda immunokromatografik test kiti ve konvansiyonel bakteriyolojik yöntemlerle etken araştırması yapıldı. İshali buzağıların %51'inde rotavirus, %35'inde Cryptosporidium, %23'ünde Cl. perfringens, %16'sında coronavirus, %4'ünde E. coli F5 (K99), %2'sinde Salmonella spp. tespit edilirken. Giardia spp. hiç bir buzağıda tespit edilmemiştir. Buzağıların %65'inde tek bir etken bulunurken, %33'ünde birden fazla etken tespit edilmiştir. Sağaltımda laktoferrinin E. coli O157, E. coli F5 ve Salmonella enteriditis üzerindeki in vitro bakterisidal ve bakteriyostatik etkisine bakıldı. Laktoferrinin 100 mg/ml ve daha yüksek konsantrasyonlarda bakteriler üzerinde etkili olduğu ve ölüm oranını düşürdüğü, ayrıca klinik parametreler üzerinde olumlu bir etki gösterdiği tespit edilmiştir. Laktoferrinin 100 mg/ml ve daha yüksek dozlarda koruyucu ve tedavi amaçlı kullanılabileceği ve sağaltımda daha etkili olacağı kanaatine varılmıştır.
Prevalence of Four Enteropathogens With Immunochromatographic Rapid Test in the Feces of Diarrheic Calves in East and Southeast of Turkey
(Univ Agriculture, Fac veterinary Science, 2013) Icen, Hasan; Arserim, Neval Berrin; Isik, Nurettin; Ozkan, Cumali; Kaya, Abdullah
In this study, fecal specimens taken from 192 diarrheic and 14 healthy calves (2-40 days old) were examined for the presence of bacterial and parasitic agents. Fecal samples from diarrheic calves with the four immunochromatographic rapid tests were 92.7% positive for four enteropathogens. The individual prevalence was 25, 21.8, 9.4 and 2.1% for Rotavirus, Cryptosporidium parvum, E. coli K99 and Coronavirus, respectively. Concomitant infections caused by two agents were 15.6% for Rotavirus+Cryptosporidium, 1.0% for Rotavirus+Coronavirus, 5.2 % for Cryptosporidium+E. coli K99, and 7.3% for Rotavirus+E. coli K99. Besides concomitant infections caused by three agents were 3.1% for Cryptosporidium +Rotavirus+E. coli K99 and 1.0%, Cryptosporidium+Rotavirus+Coronavirus. In addition one calf (1.0%) was infected by combination of four agents as Cryptosporidium, Rotavirus, Coronavirus, and E. coli K99. The calculated individual prevalence was 56.9% for Rotavirus, 47.8% for C. parvum, 26.0% for E. coli K99 and 5.2% for Coronavirus. However, 88 samples were positive in smear detection for Cryptosporidium while 92 were positive in rapid test. As a result of this study it can be concluded that multiple etiologies of diarrhea can be seen and this can help in the development of a specific treatment and preventative measures for practitioners in east and southeast of Turkey. (C) 2013 PVJ. All rights reserved
Serum Malondialdehyde, Coenzyme Q10 and 8-Hydroxy Levels in Calves With Foot-And Disease
(Polish Soc veterinary Sciences Editorial office, 2018) Ozkan, Cumali; Huyut, Zubeyir; Yildirim, Serkan; Ozbek, Mustafa; Icen, Hasan
Foot-and-mouth disease (FMD) is an acute, contagious viral disease in cattle that is associated with enormous economic losses in Turkey and worldwide. The purpose of this study was to determine changes in serum malondialdehyde (MDA), coenzyme Q10 (CoQ10), 8-hydroxy-2-deoxyguanosine (8-OHdG) and deoxyguanosine (dG) and to perform histopathological examinations in calves with FMD. Thirty calves were studied, 20 of which were infected with FMD and 10 were free of the disease. Following a routine clinical examination, blood samples were obtained, and serum MDA, CoQ10, 8-OHdG and dG levels were determined. Necropsy and histopathological examinations were performed on dead calves with FMD. MDA and 8-OHdG/ 106dG levels were significantly higher in calves with FMD than in the control group. However, the increase in CoQ10 levels in calves with FMD, compared with the control group, was not statistically significant. Macroscopic examination of the heart tissue of calves with acute myocarditis revealed the presence of pale, yellowish gray-white necrotic muscle fibers in the ventricular wall of the heart. The muscle fibers in the myocardium were swollen and exhibited pyknotic nuclei and intense lymphocytic cell infiltration. In longitudinal sections, the muscle fibers were non-striated, swollen, and homogenously pink and contained pink nuclei. Between muscle fibers, intense mononuclear cell infiltration was observed. The findings of the present study indicate that oxidative stress is significantly increased in calves with FMD, and that oxidative DNA damage may play an important role in the etiopathogenesis of FMD. This is the first study to report CoQ10 and 8-OHdG levels in calves with FMD, and its findings may serve as the basis for future studies on this subject.