Browsing by Author "Ilhan, F."

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Anencephaly and Acrania in an Akkaraman Lamb: a First Clinical Case Report
(Wiley-blackwell, 2015) Cakmak, G.; Yildirim, S.; Eski, F.; Gul, Y.; Ilhan, F.; Sendag, S.
The Effects of Sildenafil Citrate and Vitamins A, C and E on Testicular Damage in Alloxan-Diabetic Rats
(Medwell Journals, 2012) Uslu, B.A.; Ilhan, F.; Gulyuz, F.; Karasu, A.; Tas, A.; Sendag, S.; Wehrend, A.
Sildenafil citrate is an active ingredient used successfully in the treatment of erection disorders caused by a variety of factors. The purpose of this study is to research the effects of Sildenafil citrate and vitamins A, C and E on testicular degeneration in rats with alloxan diabetes. The rats were divided into II groups; healthy control and diabetic groups. The diabetic rats were divided into X groups with ten animals in each group. Group I consisted of control diabetic rats that were given only distilled water, Group II was the diabetic group treated with glibenclamide; Group III was the diabetic group treated with insulin, Group IV was the diabetic group treated with Sildenafil citrate, Group V was the diabetic group treated with Vitamin A, Group VI was the diabetic group treated with vitamine, Group VII was the diabetic group treated with vitamin E, Group vni was the diabetic group treated with Sildenafil citrate and vitamin A, Group IX was the diabetic group treated with Sildenafil citrate and vitamin C and Group X was the diabetic group treated with Sildenafil citrate and vitamin E. Testis tissue samples were collected on the 3, 7 and 15th days of treatment in control and diabetic groups. In the histopathological examination of the testicles of diabetic rats, the seminiferous tubules and interstitium of testes in the control groups were normal and the complete spermatogenic cells in the seminiferous tubules were healthy and uniformly arranged. Severe degeneration and tubular atrophy was observed in the seminiferous tubules on the 3, 7 and 15th days in the group with induced alloxan diabetes given only distilled water (Group I). The lesions on day 3rd in the diabetic group given glibenclamide (Group II) were similar to those in the diabetic control group given only distilled water. All of the tubules had been affected by the 3rd day in group three which was given insulin. A small number of spermatogenic cells and giant cells were observed in some tubules. However, almost al of the degenerated tubules had healed on the 7 and 15th days. Even though, recovery on day 15th was very similar in Groups IV-X recovery was much more pronounced in Groups VIII-X beginning from day 3rd. © Medwell Journals, 2012.
Immunohistochemical Detection of Mannheimia (Pasteurella) Haemolytica Antigens in Goats With Natural Pneumonia
(Springer, 2009) Yener, Z.; Ilhan, F.; Ilhan, Z.; Saglam, Y. S.
Pneumonia is a leading cause of loss to ruminants throughout the world. Mannheimia (Pasteurella) haemolytica is one of the most important etiological agent of pneumonia in cattle, sheep, and goats. This study was carried out to determine the incidence of M.haemolytica antigens using immunohistochemistry labelling of formalin-fixed, paraffin-embedded tissues in pneumonic lungs of goats slaughtered at abattoir, and then to compare these immunohistochemistry results with the results of bacterial isolation. For these objectives, a total of 1505 goat lungs slaughtered in slaughterhouse were grossly examined and pneumonia was detected in 74 cases (4.91%). Of these, with the exception of verminous pneumonia observed in 32 cases, on 42 pneumonic lungs immunohistochemical examinations were performed. Formalin-fixed and paraffin-embedded lung tissue samples were immunohistochemically stained by the avidin-biotin-peroxidase complex (ABC) procedure using polyclonal antibodies to detect M.haemolytica antigens. Pneumonic lesions were more frequently encountered in cranioventral lobes than caudal lobes, and characterized by irregular lobular foci of atelectasis or lobar pneumonia. The presence of M.haemolytica antigens was detected in 19 (45%) out of 42 pneumonic lungs. Bacterial antigens were found most frequently in the cytoplasm of bronchial and bronchiolar epithelial cells, in the swirling degenerating leukocytes in the alveoli, and in the degenerating leukocytes in the area of coagulation necrosis, less frequently in the epithelial cells of bronchial glands, and lymphoid cells. Conclusionly, immunohistochemical detection of M.haemolytica antigens in pneumonic lungs appear to be more reliable compared to bacterial isolation.
Immunohistochemical Detection of Pcna and P53 in Mammary Tumours and Normal Tissues in Dogs
(Ecole Nationale veterinaire Toulouse, 2008) Ilhan, F.; Metin, N.; Birincioglu, S. Turkutanit
In this study, the expression of proliferating cell nuclear antigen (PCNA) and p53 proteins was investigated in canine mammary tumours and in healthy tissues by immunohistochemistry using the PC10 anti-PCNA antibody and the DO7 and PAb240 anti-p53 antibodies. A total of 46 cases (19 benign lesions: hyperplasia, adenomas and benign mixed tumours and 27 malignant lesions: carcinomas and malign mixed tumours) were examined. No PCNA and p53 staining was evidenced in healthy mammary tissues. The frequency of nuclear PCNA positive staining was higher among malignant tumours (88.9%) than in benign lesions (52.6%) in which the staining extent was always low (< 10%). By contrast, the proportions of PCNA positive cells were moderate (> 10%) to high (> 50%) in 75% of the positive malignant tumours, mainly in solid adenocarcinomas and in papillar adenocarcinomas at a lesser extent. Whereas the DO7 antibody failed to detect any p53 accumulation, nuclear and cytoplasm p53 staining was evidenced using the Pab240 antibody in 15.8% and 48.1% of benign and malignant tumours respectively. The p53 staining remained weak (< 10%) in benign lesions and was more intense in malignant tumours mainly in adenocarcinomas (66.7% of positive solid adenocarcinomas exhibited more than 10% of positive cells). Moreover, p53 positive tumours were also PCNA positive except for one case. These results show that PCNA and p53 may be useful markers for evaluating malignancy in canine mammary tumours.
Mhc Class Ii Plus (hla-Dp Cells in the Cow Reproductive Tract: Ii. Immunolocalization of Mhc Class Ii Plus Cells in Oviduct and Vagina
(Wiley-blackwell, 2009) Eren, U.; Kum, S.; Sandikci, M.; Eren, V.; Ilhan, F.
P>The aim of this study was to determine and examine the distribution of major frequency MHC II+ cells in the oviduct and vagina of cows during the oestrous and dioestrus phases. Right oviduct (ampulla, isthmus) and vaginal samples taken from a total of twenty seven multiparous cows were used. Tissue samples were processed to obtain both cryostat and paraffin sections. Sections were stained immunocytochemically using StreptABC method using a specific monoclonal antibody to MHC II+ cell population. Intra-epithelial and subepithelial areas along with lamina propria, muscularis mucosae and serosa of both ampulla and isthmus and intra-epithelial/subepithelial areas and mucosae of vagina were examined for the presence of MHC II+ cells. The density of immune positive cells was determined using a subjective scoring system. MHC II+ cells were demonstrated in all areas examined in both oestrus and dioestrus. In oestrus, the density of MHC II+ cells decreased in subepithelial areas (in between the epithelial cells and the basal membrane) of isthmus, whereas the density of immune positive cells was increased in muscularis mucosae of isthmus (P < 0.05), lamina propria and muscularis mucosae of ampulla (P < 0.05) as well as in the mucosae of vagina (P < 0.005). This study indicates that the density of MHC II+ cells observed in the oviduct and vagina increases in the majority of areas examined due to the effect of oestrogen.
Oxidative Stress in Rats During Exposure To Cigarette Smoke and After Smoking Cessation
(Wiley-blackwell, 2016) Donder, A.; Balahoroglu, R.; Huyut, Z.; Yildirim, S.; Dulger, H.; Ilhan, F.; Yilmaz, A.
Pathology and Phylogenetic Analysis of Capripoxvirus in Naturally Infected Sheep Sheeppox Virus
(Univ Agriculture, Fac veterinary Science, 2017) Karapinar, Z.; Ilhan, F.; Dincer, E.; Yildirim, S.
Sheeppox and goatpox is a contagious viral disease of sheep and goats characterized by fever, generalized papules or nodules in skin and mucosal surface. DNA virus belonging to the genus capripoxvirus. The disease with high mortality and morbidity causes significant economic losses in small ruminants. In this study, 20 tissues from 8 sheep which were considered having natural sheeppox based on the macroscopic and histopathologic evaluation were further investigated by immunohistochemical (iNOS, SP-A, HSP-70), and Polymerase Chain Reaction (PCR) method. Papules, typically pox lesions, noticeable on the skin surfaces were examined macroscopically. Immunohistochemically; iNOS and SP-A were most intensely stained whereas HSP-70 was low stained. PCR method was used for the detection of A29L gene of capripoxvirus. Positive samples obtained from sheep were used for molecular characterization. A phylogenetic analysis was performed using sequence of the partial A29L gene and by comparing with reference sheeppox viruses isolates obtained from Gene Bank. The results of the sequence analysis were similar among themselves, they were found different (99-100% identity) from the other sheeppox viruses around the world. This study provides firstly phylogenetic analysis of sheeppoxviruses from Van province in Turkey. (C) 2016 PVJ. All rights reserved
Prevalence of Paramphistomum Infection in Cattle and Sheep in Van Province, Turkey
(versita, 2010) Ozdal, N.; Gul, A.; Ilhan, F.; Deger, S.
This study was carried out in naturally infected cattle and sheep from May, 2006 to April 2007 in Van province, in the eastern of Turkey, to assess the prevalence of paramphistomosis. The rumen and reticulum of slaughtered cattle and sheep were collected monthly from abattoirs (Van Municipality of Slaughterhouse) to monitor the seasonal occurrence of paramphistomosis. 447 cattle and 948 sheep were examined, out of which 8.95 and 4.43 % were positive for Paramphistomum infection, with the mean intensity +/- standard error of mean of 97.55 +/- 12.50 and 89.17 +/- 13.02, respectively. The highest infection in the cattle and sheep infected with Paramphistomum spp. was found during the autumn (September to November) (14.10 %, 8.33 %) and fallowed by the summer seasons (June to August) (9.02 %, 5.18 %).
Pulmonary Myxoma in a Sheep
(Sage Publications inc, 2009) Ilhan, F.; Yener, Z.
Pulmonary myxoma is an uncommon neoplasm. A pale tan, lobulated, and well-circumscribed mass was discovered at slaughter in the left lung of a 5-year-old sheep. Histologically, the tumor was composed of spindloid to stellate cells in a myxoid matrix. Neoplastic cells were immunohistochemically positive for vimentin but did not express cytokeratins, S-100 protein, smooth muscle actin, desmin, or p53. On the basis of the histologic and immunohistochemical findings, this tumor was diagnosed as a myxoma.