Browsing by Author "Ilhan, Fatma"

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Assessment of the Histopathological Changes Occurring in the Testis of the Mice Suffering From Experimental Diabetes Induced Using Alloxan
(Medwell online, 2009) Uslu, Baris Atalay; Ilhan, Fatma; Gulyuzf, Fetih; Karaca, Mehmet; Oner, Ahmet Cihat
In this study, we aimed to examine the effect of volatile oil extracts of Helichrysum plicatum DC (HP) and Tanacetum balsamita L. (TB) on the histopathology of the testicles of the mice that had alloxan-induced type 1 diabetes or with imitated physiopathology of type 2 diabetes. The volatile oil extracts of HP and TB; were injected intra. peritoneally in diabetic mice at 12.5, 25 and 50 mg kg(-1) doses. Control and reference groups received 0.2 mL serum physiologic intra peritoneally and oral glibenclamide at 0.3 mL kg(-1) dose, respectively. Blood samples were withdrawn from tail veins of the mice 1, 2 and 24 It after the treatment and the blood glucose levels were measured. We investigated testicular histopathology of the mice in control and diabetic groups. The examination of the testicles of diabetic mice revealed reduction in diameters of the seminiferous tubules and thickening in the wall of the seminiferous tubules in addition to degenerative changes and decline in the number of spermatogenic cells. There were multinucleated giant cells in the lumens of some seminiferous tubules. No significant shown was detected in the severity of lesions between control group of diabetic mice receiving serum physiologic and the diabetic mice treated with the extracts of H? and TB. TUNEL-positive cells were higher diabetic mice than in control mice. We monitored no marked encouraging effect of HP and TB volatile oil extracts on the testicular histopathology. The reason for this failure might be due to short term use of these extracts. Long term applications of volatile oil extracts of HP and TB at various doses remained to be done to elucidate the potential anti-diabetic effect of these extracts.
Bir Sokak Kedisinde İntrakraniyal Transisyonel Meningiom Olgusu
(2010) Ilhan, Fatma; Gül, Yusuf
Bu çalışmada; 12 yaşlı, erkek bir sokak kedisinin sol pariyetal lobunda saptanan transisyonel meningiom olgusu makroskobik, mikroskobik ve immunohistokimyasal bulgularına göre tanımlandı. Makroskobik olarak kitle sarımsı renkte, sert ve 0.4x0.2x0.3 cm boyutlarındaydı. Histopatolojik incelemede, iğ şeklindeki tümör hücre kümelerinin konsantrik iç içe dizilmiş (dalgalı demetler) ve düğümler şeklinde helezonik adacıklar oluşturduğu, bu hücre adacıklarının bazılarının merkezinde ise kireçlenme şekillendiği (psammom cisimciği) saptandı. İmmunohistokimyasal incelemede tümör hücreleri, vimentin için immunopozitif, sitokeratin ise negatif olarak belirlendi. Bu sonuçlara göre tümör, transisyonel meningiom olarak değerlendirildi.
Diagnosis and Treatment of Umbilical Cord-Derived Tetanus in Neonatal Calves
(Tubitak Scientific & Technological Research Council Turkey, 2017) Altug, Nuri; Yuksek, Nazmi; Karasu, Abdullah; Ilhan, Fatma; Ceylan, Ebubekir; Ekin, Ismail Hakki; Arslan, Sezai
In this study, umbilical cord-derived neonatal tetanus in calves was identified in Turkey. Four calves with tetanus-specific history and clinical findings were used. Blood samples were taken before and after treatment, and clinical findings were recorded. A tetanus-specific treatment procedure was performed. However, the calves died from not responding to treatment. Pretreatment white blood cell, peripheral blood polymorphonuclear leukocyte, creatine kinase (CK), and lactate dehydrogenase (LDH) levels were determined to be high. Aspartate aminotransferase, alanine aminotransferase, CK, and LDH activities were found to increase after treatment compared to before treatment. Gram-positive terminal spore-forming bacilli were observed in bacterioscopic examination of the necrotic tissue and swab samples from the umbilical cord region. The umbilical cord region was determined to be infected in all calves. In histopathological examination, pyogranuloma formation was observed in the wound area, and in Gram staining agents morphologically concordant with Clostridium tetani in necrotic material were observed. Blood serum of the calves was inoculated into mice. All mice died within 2 days after the inoculation, showing tetanus-specific clinical findings. As a result, bacterioscopy and histopathology of the umbilical cord region may be useful for diagnosis in addition to clinical findings. Mice trials may be used in confirming the diagnosis.
Effects of Diclofenac Sodium on the Rat Liver in Postnatal Period
(Medwell online, 2009) Ragbetli, Cennet; Aydinlioglu, Atif; Kara, Mikail; Ragbetli, Murat Cetin; Ilhan, Fatma
Diclofenac Sodium (DS) is a nonsteroidal anti-inflammatory drug used in painful conditions, trauma, dysmenorrhoea, rheumatoid arthritis, degenerative joint disease and ankylosing spondylitis. There is the paucity of the research investigating DS effect on the rat liver in the fetal period. The present study aimed to investigate the possible postnatal effects of DS on the liver tissues of the offspring of rats. DS was administered to pregnant rats for 15 days from the 5th-20th day of pregnancy. After mating day, pregnant female rats were separated into the control and DS treated groups. DS (1 mg kg(-1) daily) was injected intraperitoneally to the drug-treated group and physiological saline (1 mL kg(-1)) to the control group. Liver tissues were collected from pups at the 20th week and paraffin sections were dyed with hematoxylen-eosin. DS caused proliferation of bile ducts, enlargement of periportal area, dilatation of sinusoids and the central vein, parenchymal degeneration and mononuclear cell infiltration. Significant changes between the control and the treated groups were found (p<0.05). However, no significant difference was observed between the sexes in both groups. This study using DS with low dose did not show parenchymal cell death, as a different finding from the previous studies. However, these observations need to be confirmed by further studies using different doses of DS during pregnancy.
Effects of Urtica Dioica L. Seed on Lipid Peroxidation, Antioxidants and Liver Pathology in Aflatoxin-Induced Tissue Injury in Rats
(Pergamon-elsevier Science Ltd, 2009) Yener, Zabit; Celik, Ismail; Ilhan, Fatma; Bal, Ramazan
This study was carried out to investigate the hepatoprotective and antioxidant properties of Urtica dioica L seeds (LIDS) extract against aflatoxin (AF)-exposure in rats. The preventive potential and antioxidant capacity of the plant's extract was evaluated by liver histopathological changes, measuring serum marker enzymes, antioxidant defense systems and lipid peroxidation (Malondialdehyde, MDA) content in some tissues of rats. Eighteen rats were randomly divided into one of three experimental groups: control, AF-treated group and AF+UDS-treated group. Rats in control group were fed with a diet without AF. Rats in AF-treated group and AF+UDS-treated group received approximately 25 mu gr of AF/rat/day. AF+UDS groups also received 2 mL of LIDS oils/rat/day by gavage for 90 days. Administration of LIDS extract restored the AF-induced imbalance between MDA and antioxidant system towards near normal particularly in liver. Hepatoprotection by LIDS is further substantiated by the almost normal histologic findings in AF+UDS-treated group as against degenerative changes in the AF-treated rats. It is concluded that LIDS has a hepatoprotective effect in rats with aflatoxicosis, probably acting by promoting the antioxidative defense systems. (C) 2008 Elsevier Ltd. All rights reserved.
Expression of P53 Protein, Jaagsiekte Sheep Retrovirus Matrix Protein, and Surfactant Protein in the Lungs of Sheep With Pulmonary Adenomatosis
(Sage Publications inc, 2016) Ilhan, Fatma; Vural, Sevil A.; Yildirim, Serkan; Sozdutmaz, Ibrahim; Alcigir, Mehmet E.
Ovine pulmonary adenocarcinoma (OPA) is a naturally occurring cancer in sheep that is caused by the Jaagsiekte sheep retrovirus (JSRV). Because the pathologic and epidemiologic features of OPA are similar to those of bronchoalveolar carcinoma in humans, OPA is considered a useful animal model for pulmonary carcinogenesis. In this study, 3,512 lungs from various breeds of sheep were collected and macroscopically examined. OPA was identified in 30 sheep, and samples of these animals were further examined by histologic, immunohistochemical (p53 protein, surfactant protein A [SP-A], proliferating cell nuclear antigen [PCNA], JSRV matrix protein [MA]), and PCR methods. Papillary or acinar adenocarcinomas were detected microscopically in the affected areas. Immunoreactivity for p53 PAb240 was detected in 13 sheep, whereas p53 DO-1 was not detected in any of the OPA animals. PCNA immunoreactivity was recorded in 27 animals. SP-A and JSRV MA protein was immunopositive in all 30. JSRV proviral DNA was detected by PCR analysis in all of the lung samples collected from OPA animals. In addition, the pulmonary SP-A levels were increased in tumor cells. The results of this study suggest that PCNA and p53 protein expression may be useful indicators in monitoring malignancy of pulmonary tumors.
A Histological Investigation Concerning the Effects of Diclofenac Sodium To the Lung in 4-And 20-Week Rats Treated Prenatally
(informa Healthcare, 2011) Ragbetli, Cennet; Ilhan, Fatma; Aydinlioglu, Atif; Kara, Mikail; Ragbetli, Murat C.
Objective. We aimed to investigate the possible postnatal effects on the lung tissues of the rat offspring treated with diclofenac sodium (DS) during pregnancy. Methods. After mating, pregnant female rats were separated into the control (n = 10) and DS (n = 10) groups. DS (1 mg/kg) was injected intraperitoneally (i.p) to the drug-treated group for the period of gestational days 5-19. Physiological saline (1 ml, i.p.) was given to the control groups. After birth, pups were separated into DS treatment groups (n=24) and control group (n=24). The DS and control group animals were anaesthetised with i.p. injection of urethane and their lungs were removed to prepare for histopathological evaluation. Results. Histological examination of the lung tissues of the 4- and 20-week-old rats revealed no significant differences between males and females in both the control and DS treated rats. Conclusion. Because of the use of DS in the pregnant women further studies are needed in this field.
Immunohistochemical Detection of Brucella Melitensis Antigens in Cases of Naturally Occurring Abortions in Sheep
(Amer Assoc veterinary Laboratory Diagnosticians inc, 2008) Ilhan, Fatma; Yener, Zabit
Brucella melitensis, a worldwide zoonotic pathogen, is a significant cause of abortion in sheep and goats in some countries. The present Study was carried out to determine, by immunohistochemistry, the presence of B. melitensis antigens in 110 naturally occurring aborted sheep fetuses. Sections Of lung, liver, kidney, and spleen of each fetus were stained with immunoperoxidase to detect Brucella antigens. Brucella melitensis antigens were detected in 33 of 110 fetuses (30%). In the 33 positive cases, Brucella antigens were found in lung (25 [22.7%]), liver (21 [19%]), spleen (13 [11.8%]), and kidney (6 [5.4%]). Microscopic Studies demonstrated that Brucella antigens were mainly located in the cytoplasm of macrophages and neutrophils of the lung, and in the cytoplasm or macrophages in the portal infiltrates and Kupffer cells of the liver. It was Concluded that immunohistochemistry in formalin-fixed, paraffin-ern bedded tissues is a useful tool for the diagnosis of spontaneous ovine abortion caused by B. melitensis.
Investigation of Selected Bacterial Agents Causing Sheep Abortion in the Van Province by Rt-Pcr and Histopathological Methods
(veterinarni A Farmaceuticka Univerzita Brno, 2023) Gulaydin, Ozgul; Ozturk, Cihat; Ekin, Ismail Hakki; Ilhan, Ziya; Ilhan, Fatma
Abortion causes significant economic losses in the sheep industry. Determination of the aetiology is important in dealing with abortions. The present study was aimed to identify selected important bacterial pathogens in the abortion cases of sheep. A total of 113 samples (105 aborted sheep foetuses, 4 placentas, and 4 vaginal swab samples) from 85 different sheep flocks were examined by real-time PCR (RT-PCR) regarding Chlamydia (C.) spp., C. abortus, Brucella (B.) spp., B. melitensis, Salmonella (S.) spp., S. Abortusovis, Coxiella (C.) burnetii, Listeria (L.) spp., L. monocytogenes, and Campylobacter spp. All cases that were found to be positive for bacterial agents by RT-PCR, were examined pathologically. Tissue samples of foetuses that were found to be positive for B. melitensis and L. monocytogenes by RT-PCR were also investigated immunohistochemically. A total of 35 (30.9%) samples were found to be positive by RT-PCR, with 15 (42.8%), 9 (25.7%), 5 (14.2%), 4 (11.4%), 1 (2.8%), and 1 (2.8%) of them being identified as C. abortus, B. melitensis, S. Abortusovis, C. burnetii, L. monocytogenes and Campylobacter spp., respectively. The presence of the antigen was confirmed also immunohistochemically in the cases with B. melitensis and L. monocytogenes. As a consequence, C. abortus was found to cause the highest rate of sheep abortion cases, which should be taken into account when implementing control measures in epidemiological investigations.
The Investigation of The Presence of Some Bacterial and Viral Agents in Pearl Mullet (Chalcalburnus Tarichii, Pallas 1811) by Real-Time Pcr and The Histopathological Examination
(Parlar Scientific Publications (p S P), 2018) Gulaydin, Ozgul; Ozturk, Cihat; Onalan, Sukru; Karapinar, Zeynep; Arabaci, Muhammed; Ekin, Ismail Hakki; Ilhan, Fatma
The aim of this study was to identify some bacterial (Vibrio cholerae, Listonella anguillarum, Yersinia ruckeri, Aeromonas hydrophila, Aeromonas sobria and Aeromonas caviae) and viral (infectious pancreatic necrosis virus, viral hemorrhagical septicemia virus and infectious hematopoietic virus) pathogens by comparing the bacteriological culture and molecular methods with histopathological examination of various tissues seen in Pearl mullet from freshwater rivers inflowing to the Van lake. Accordingly, bacterial and viral agents were detected in a total of 180 pearl mullet samples by bacteriological culture and Real-Time PCR methods. The samples were collected from six different freshwater rivers that flows to Van lake by random sampling. In the study, Vibrio cholerae, Listonella anguillarum, Yersinia ruckeri, Infectious Pancreatic Necrosis Virus, Viral Hemorrhagic Septicemia Virus and Infectious Hematopoietic Necrosis Virus could not be isolated. However, Aeromonas spp. was identified by bacteriological culture and Real Time PCR methods at 22.22% and 53.33% respectively. The most dominant species was observed to be Aeromonas hydrophila among all identified Aeromonas spp. In histopathological examinations of Aeromonas spp. positive samples, disorders were detected in gill, liver, spleen and kidney tissues. Telangiectasia, edema, hyperplasia and adhesions were observed on the seconder laminates of gills. In liver, the order of the Remak cords were disarranged, and degenerative changes formed in hepatocytes. Melano-macrophages loaded with hemosiderin were intensively detected in all Aeromonas positive samples. As a result, the risk of motile Aeromonas infections in the pearl mullet living in freshwaters flowing into the Van lake is still highly and this could lead to serious adverse effects on human health. To prevent these effects, attention must be paid to hygiene conditions and cold chain in fish served for consumption, the and rivers must be protected from pollution.
Investigation of the Seroprevalance of Maedi-Visna in the Region of Van Using Elisa and Histopathological Findings
(Medwell online, 2009) Akkan, Hasan Altan; Karaca, Mehmet; Tutuncu, Mehmet; Keles, Ihsan; Ilhan, Fatma; Cetin, Yunus; Tasal, Ibrahim
In the present study, seroprevalance of maedi-visna in sheep in the region of Van was aimed to investigate. A total of 465 serum samples obtained from sheep raised in Van and its towns were investigated with regard to maedi-visna specific antibody using ELISA procedure. Out of 465 samples 30 of them were seropositive (6.45%) and 20 of them were doubtful (4.30%) according to test procedure. Histopathology was also applied to some of these sheep which slaughtered in an abattoir after blood sampling. According to histopathological analysis 9 doubtful samples were also found to be positive. But, due to mild lymphoproliferation in there samples ELISA gave doubtful results. As a result with this study, seroprevalance of maedi-visna was determined in the region of Van and in the doubtful cases histopathological assessments believed to strengthen serological findings.
Matrix Metalloproteinase Expression in Sheep With Listerial Meningoencephalitis
(Elsevier Sci Ltd, 2012) Ilhan, Fatma; Ulusoy, Yavuz; Haligur, Mehmet
Matrix metalloproteinases (MMPs) have been implicated in the pathogenesis of several central nervous system (CNS) diseases. In this study, we investigated the presence of Listeria monocytogenes antigens and detected the expression of MMP-9 and MMP-7 in the brains of 22 sheep with clinical signs and histopathological findings characteristic of listerial meningoencephalitis. Archived sections from the brainstem, cerebrum, and cerebellum were stained for immunohistochemistry. L. monocytogenes antigens were located mainly in the cytoplasm of neutrophils and some macrophages and/or extracellularly within microabscesses of the brainstem. MMP-9 was mainly immunolocalised in the endothelial cells, microglial cells, and neurons especially in inflammatory areas. MMP-7 immunoreactivity was detected in perivascular cuffs, microglial cells, and only a few neurons. Overall, immunohistochemistry in formalin-fixed, paraffin-embedded tissues is a useful tool for the diagnosis of encephalitic listeriosis caused by L. monocytogenes, and MMP-9 and MMP-7 may contribute to the pathogenesis of listerial meningoencephalitis. (C) 2011 Elsevier Ltd. All rights reserved.
Mucinous Cholangiocarcinoma With Metastases in a Turkish Van Cat - a Case Report
(Natl veterinary Research inst, 2008) Ilhan, Fatma; Yener, Zabit; Vural, Sevil Atalay; Haligur, Mehmet
The pathomorphological and immunohistochemical features of cholangiocarcinoma in an 11-year-old female Van cat were described. At necropsy, the primary tumour was determined in the liver. The tumour was greyish-white, firm, and multinodular. The size of these nodules ranged between 1 cm and 3 cm. There were metastases in the mesentery, portal lymph nodes, peritoneum, and lung. Immunohistochemically, the tumour cells showed positive immunoreactivity for S100 and cytokeratin, and negative for a-fetoprotein. According to morphological and immunohistochemical findings, this tumour was defined as a cholangiocarcinoma.
The Prevalence of Ovine Oestrosis (Oestrus Ovis Linnaeus, 1761, Diptera: Oestridae) and Risk Factors in Eastern Turkey
(Univ Zagreb vet Faculty, 2016) Ozdal, Nalan; Tanritanir, Pinar; Ilhan, Fatma; Deger, Serdar
This study was carried out to estimate the prevalence, larval burden and some risk factors of ovine oestrosis in 328 heads of randomly selected sheep slaughtered in one year at Van, eastern Turkey. Of the total heads, 127 (38.71 %) were infested with Oestrus ovis (Linnaeus, 1761, Diptera: Oestridae) larvae. The prevalence of O. ovis was 51.68 % in summer, 40.69 % in spring, 35.80 % in autumn, and 23.61 % in winter. The differences between seasons were statistically significant (P<0.05). Out of a total of 511 larvae collected, 17 % were L1, 29 % L2, and 54 % L3 larvae. All three larval instars were observed in all study months, apart from the fact that no first-stage larvae were seen in December and March. The overall larval intensity for the infested sheep was 4.02, with 3.42 in spring, 3.80 in summer, 4.03 in autumn and 5.82 in winter. The infestation rates were 41.26 % in female and 34.42 % in male sheep. The infestation rate in sheep less than 2-years-old was 23.33 %, in those 2 to 4 years-old 36.42 % and inj those older than 4 years old 48.43 %. Sheep with dark head color had a higher risk than sheep with white and spotted heads (P<0.05).
Ratlarda Deneysel Olarak Oluşturulan Aflatoksikozisde Çörek Otu (Nigella Sativa) ve Isırgan Otu (Urtica Dioica)'nun Karaciğer Lezyonlarını Engelleyici Etkisinin Araştırılması
(2006) Ilhan, Fatma; Yener, Zabit; Celik, Ismail
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Rupture of the Gastrocnemius Muscle in a Cow Two Months After Twin Birth
(Natl veterinary Research inst, 2007) Altug, Nuri; Ozkan, Cumali; Yuksek, Nazmi; Karasu, Abdullah; Keles, Ihsan; Agaoglu, Zahid Tevfik; Ilhan, Fatma
The rupture of the gastrocnemius muscle was described in a cow who gave a twin birth. A tentative diagnosis of complete rupture of the muscle was based on information obtained from both anamnesis and clinical signs. The definitive diagnosis was made on necropsy. The role of serum minerals, especially Ca, changes in the muscle originated enzymes, and other laboratory parameters together with clinical examination findings was discussed.
Van Et ve Balık Kurumu’nda Kesilen Kıl Keçilerinde Linguatula Serrata (Fröhlich, 1789) Nimflerinin Araştırılması
(2009) Ilhan, Fatma; Gül, Abdurrahman
Bu araştırma, Van Et ve Balık Kurumu’nda kesilen kıl keçilerinin mezenteriyal lenf yumrularında Linguatula serrata nimflerinin yaygınlığını ve enfekte lenf yumrularındaki histopatolojik değişiklikleri araştırmak için yapılmıştır. Bu amaçla, 116 keçinin mezenteriyal lenf yumrusu incelenmiş, 9 keçide (%7.76) L. serrata nimfi tespit edilmiştir. Enfekte keçilerde toplam 9 adet L. serrata nimfi toplanmış olup, her mezenteriyal lenf yumrusunda birer adet L. serrata nimfi görülmüştür. Enfekte mezenteriyal lenf yumrularının histopatolojik incelenmesinde, içerisinde nimfin yer aldığı kistik yapının yangısal bir alanla çevrelendiği, lenfoid folliküllerde ise lenfositlerin azalmasına bağlı olarak lenfoid folliküllerinin merkezinin boşaldığı tespit edilmiştir.