Browsing by Author "Inal, B."

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Comparative Adaptation Responses of Melon (Cucumis Melo L.) Genotypes To Salinity Stress
(Tarbiat Modares Univ, 2021) Erdinc, C.; Inal, B.; Erez, E.; Ekincialp, A.; Sensoy, S.
The objective of this work was to understand the mechanisms of physiological, biochemical, and molecular responses to salinity stress of three Turkish melon genotypes (YYU 1, YYU 4 and CU 196) and cv. Ananas. The study used Randomized Complete Block Design (RCBD) and pots were irrigated with Hoagland nutrient solution after two-leaf stage until harvesting by 50 and 75 mM NaCl concentrations. For evaluation of responses, chlorophyll and carotenoid content, total phenolic and flavonoid amount, proline variations, and nutrient elements were determined. Moreover, qRT-PCR analyses were performed to identify the expression level of six TF (Transcription Factors) genes (WRKY24, TCP15, CmHD-Zip, mTERF2, Dof3 and CmADH2). Increase in salt application led to increase in chlorophyll content in the melon genotypes, but decrease (about 55%) in cv. Ananas. Phenolic, flavonoid, and proline contents varied based on the melon genotypes, but generally increased in Ananas. Expression levels of TCP15 and WRKY24 showed more fold change at 75 mM NaCl treatment. On the other hand, the expression of CmADH2 and Dof3 showed more fold change at 50 mM NaCl treatment. Finally, according to adaptation mechanisms of melon genotypes, the study might help in selection and detection of the salt tolerant ones.
Determination of Botanical Origin and Mineral Content of Propolis Samples From Balveren (Şırnak) Beekeepers Accommodation Areas
(ABADER (Adıyaman Bilimsel Arastırmalar Dernegi), 2022) Fidan, M.; Pinar, S.M.; Erez, M.E.; Inal, B.; Eroğlu, H.
Researches on bee products have become popular in recent years. In fact, the content and component of bee products varies depending on many ecological and floristic factors and its nutritional and therapeutic properties are directly related to its content. Balveren (Şırnak province) beekeepers place their hives in locations with different geographical structure, floristic and topographic characteristics. This variability not only affects the quality of honey but also changes the properties of propolis. Studies on propolis, known as bee glue, have gained importance in recent years. As with other bee products, the propolis content also depends on the floristic characteristics of the region. In this study, propolis samples were collected from the regions where Balveren beekeepers stayed and their botanical origins, wax ratios, phenolic content, and mineral substance contents were analyzed. In the microscopic analysis, pollen grains belonging to 14 different families used by bees were determined. It was determined that the total phenolic and mineral contents of propolis vary completely depending on the location. With this study, the propolis properties of the hives in the region were tried to be revealed and it was aimed that this study would help the region's propolis to be used for technological and therapeutic purposes. © 2022, ABADER (Adıyaman Bilimsel Arastırmalar Dernegi). All rights reserved.
Physiological and Gene-Expression Variations in Watermelon (Citrullus Lanatus L.) Cultivars Exposed To Drought Stress
(Polish Botanical Society, 2020) Erez, M.E.; Inal, B.; Karipçin, M.Z.; Altıntaş, S.
Drought conditions may have direct or indirect effects on plant physiology, biochemistry, and molecular characteristics. The purpose of this study was to investigate the effects of drought stress on the physiological, biochemical, and molecular responses of three different watermelon cultivars with varying levels of drought tolerance (24: drought resistant, CS: moderately tolerant, and 98: drought sensitive). The cultivars exhibited different responses to cope with water stress according to their tolerance level. Drought induced significant reductions in chlorophyll a, total chlorophyll and carotenoid content and glutation reductase and ascorbate peroxidase activity in the sensitive cultivar unlike in the moderately tolerant and drought resistant cultivars. Additionally, the expression levels of NAC1, NAC2, ORE1, WRKY24, SAG12, SAG13, KCS2, CER1, DREB2A, LTP3, SWEET15, and PYL9 genes were measured using qRT-PCR. The expression ratios of the genes significantly varied depending on the gene location and on the tolerance of the cultivars. Results showed that the physiology and biochemical and molecular pathways of tolerant cultivars change to adapt to drought conditions. Therefore, the drought-resistant cultivar copes with drought stress by increasing proline content and antioxidant enzyme activities, as well as by increasing the expression of specific genes. © The Author(s) 2020.