Browsing by Author "Irak, Kivanc"

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The Effect of Evening Primrose Oil (Oenothera Biennis) on the Level of Adiponectin and Some Biochemical Parameters in Rats With Fructose Induced Metabolic Syndrome
(Taylor & Francis Ltd, 2022) Mert, Handan; Irak, Kivanc; Cibuk, Salih; Yildirim, Serkan; Mert, Nihat
The effect of evening primrose oil on adiponectin level and some biochemical parameters in model of fructose-induced metabolic syndrome were investigated. The rats were divided into 4 groups: control, evening primrose oil, fructose, fructose + evening primrose oil. Body weight, daily feed and water consumptions and systolic blood pressures of animals were measured. At the end of trial, blood samples were taken, livers were excised and histopathological examination was performed. Glucose, uric acid, triglyceride, T.cholesterol, LDL, HDL, VLDL, ALT, AST, ALP, LDH, adiponectin, insulin, IL-6, TNF-alpha, TAC, and TOS levels were analysed. Some analysed parameters and systolic blood pressure of fructose + evening primrose oil group decreased significantly compared to fructose group and adiponectin, TAC, and HDL levels were significantly increased. As conclusion, evening primrose oil can be considered as antioxidant agent by reducing oxidative stress, increasing adiponectin levels and insulin sensitivity, anti-inflammatory properties, exhibiting anti-atherogenic effect by regulating dyslipidemia and systolic blood pressure.
The Effect of Fucoidan on the Changes of Some Biochemical Parameters and Protein Electrophoresis in Hepatotoxicity Induced by Carbontetrachloride in Rats
(Agricultural Research Communication Centre, 2018) Aysin, Nesrullah; Mert, Handan; Mert, Nihat; Irak, Kivanc
Fucoidan is a sulfate polysaccharide extracted from brown algae. Fucoidan has various pharmacological properties, such as anti-tumor, anti-mutagenic, anti-inflammatory, antiviral, antioxidant, anti-fibrogenic activity and anti-complementary activities Objective: This study was aimed to explore the effect of fucoidan on biochemical parameters (ALT, AST, GGT, total protein, albumin, globulin) and protein fractions in hepatotoxicity induced by CCl4 in rats. Materials and Methods: The rats used in the study were randomly divided into four groups of 8 rats each: Control group, fucoidan group, fucoidan+CCl4 group and CCl4 group. After 24 hours from the process of an eight-day experiment, blood samples were taken. The analysis of ALT, AST, GGT activities and total protein, albumin, globulin levels were done by an autoanalyser and serum protein fractions (albumin, alpha 1- globulin, alpha 2-globulin beta- globulin, gamma- globulin and A/G ratio) were electrophoretically determined. Results: In the group of fucoidan+CCl4, it was determined that the levels of AST (p<0.001), GGT (p<0.001), total protein (p<0.01), globulin (p<0.01), beta-globulin % (p<0.01), gamma-globulin % (p<0.001) statistically decreased compared to CCl4 group. Conclusion: It can be said that fucoidan has the property of hepatoprotectant by looking to some biochemical parameters and changes in protein fractions that examined in hepatotoxicity induced by CCl4.
The Effect of Fucoidan on The Gentamicin Induced Nephrotoxicity in Rats
(Parlar Scientific Publications (p S P), 2018) Yilmaz, Mesut; Mert, Handan; Irak, Kivanc; Erten, Remzi; Mert, Nihat
Possible preventive or therapuetic effects of fuciodan, which has antioxidant properties and was administered together with gentamicin (GM) made up nephrotoxicity through giving GM to the rats, have been searched. The rats used in this study were randomly grouped in three: control, GM (80 mg/kg/day/i.p GM was administered for 8 days), GM+fucoidan (80 mg/kg/day/i.p GM for 8 days + fucoidan 100 mg/kg/day/ for 8 days were administered with intra-gastric gavage) groups. Blood and tissue samples were taken 24 hours after the 8 days trial period. Besides determining plasma creatinine, BUN, sodium, potassium, chloride levels in auto-analyser, whole blood glutathione, plasma MDA and AOPP levels were analyzed spectrophotometrically. The highest plasma creatinine, BUN, potassium, AOPP and MDA levels were found in the GM group. It was determined that while the levels of creatinin (p<0.01), BUN (p<0.05), potassium (p<0.05), AOPP (p<0.01), MDA (p<0.01) in GM+fucoidan group were decreasing with regard to those in GM group, chloride levels were rising (p<0.05). The study revealed the nephroprotective effect of fucoidan. These findings suggest that fucoidan administration together with GM may attenuate renal dysfunction and structural damage through the reduction of oxidative stress in the kidney.
Evaluation of Various Biochemical Parameters in the Serum and Tissues of Lambs With White Muscle Disease
(Agricultural Research Communication Centre, 2021) Irak, Kivanc; Mert, Handan; Mert, Nihat; Aysin, Nesrullah; Sogutlu, Inci Dogan; Comba, Arzu
Background: In this study, the effects of White Muscle Disease (WMD) on certain blood parameters, MDA and GSH levels in liver and thyroid tissues and the thyroid hormones were investigated in sheep. Methods: Sixteen lambs (8 with WMD, 8 controls) of similar age, 3 to 50 days old, selected from the same region were used in the study. Blood samples were taken from all animals. One ml Vitamin E + Se were injected (I/M) to the lambs with WMD and blood and tissue samples were collected after the treatment as well. The serum Ca, P, T3, T4, albumin, globulin, total protein, glutathione (GSH) values were analyzed by an auto-analyzer. Malondialdehyde (MDA) and GSH amounts of the liver and thyroid tissues were also determined. Result: The low Ca ion amount in lambs with WMD was found to have increased after injections of vitamin E + Se. P levels followed the same pattern. Total protein, albumin and globulin levels increased after treatment. It was determined that there was a decrease in T3 levels and an increase in T4 levels in patient lamb sera. The levels of GSH in both the liver and thyroid tissues were found to have decreased and statistical significance (at what level) was only found for the liver GSH levels. MDA levels in the hepatic and thyroid tissues were found to have slightly increased in the WMD group. In conclusion, serum differences in Ca, P, albumin, globulin, total protein, T3, T4, GSH, GSH and MDA levels were statistically significant between the groups.
Grape Seed Extract Effects on Serum Amylase Levels and Immunohistochemical Alterations in Streptozotocin-Induced Diabetic Rats
(C M B Assoc, 2018) Irak, Kivanc; Yildirim, Serkan; Mert, Handan; Mert, Nihat
In this study, serum amylase activity and structural changes of the pancreatic tissue in rats under the effects of grape seed extract were investigated. Thirty-two female Wistar albino rats were divided into 4 groups. First one was the control group. The second group was the streptozotocin (STZ)-induced diabetes mellitus (DM) group (45 mg/kg), while the third group was the grape seed extract (GSE) group, where the GSE was administrated intragastrically for 20 days (at 0.6 ml/rat). Lastly, the fourth group was the diabetes mellitus+GSE (DM+GSE) group. Blood samples were taken and analyzed for amylase activity. Caspase 3 expressions were inspected with immunohistochemistry. Amylase levels in the diabetic group were found to be the lowest (794.00 +/- 44.85 U/L, p<0.001), while the GSE group had the highest value (1623.63 +/- 80.04 U/L, p<0.001) Number of apoptotic cells was increased in Langerhans islets of the diabetic group. In the control and GSE groups, the apoptotic cells were found to be almost entirely absent. Increased number of apoptotic cells was found in the DM group, while decreased number of apoptotic cells was found in the DM+GSE group. Furthermore, atrophy in Langerhans islets, hyperemia in capillary veins, hydropic degeneration and necrosis in islet cells were determined in the diabetic group. Only mild hydropic degeneration in islet cells of Langerhans was observed in the DM+GSE group. Histopathologically beneficial changes in the pancreases were detected when grape seed extract was given to diabetic rats. As a conclusion, GSE was determined to have positive effects on the function and structure of the pancreas, improving enzyme activities and the structure of the Langerhans islets.
Investigation of Some Biochemical Parameters in Sheep Naturally Infected With Cystic Echinococcosis
(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2019) Irak, Kivanc; Celik, Ozgur Yasar; Celik, Burcak Aslan; Bolacali, Memis; Mert, Handan; Mert, Nihat
This study was performed to reveal the changes in certain serum biochemical parameters in animals diagnosed with Cystic Echinococcosis. The material of the study consisted of 40 sheep aged 1-3, which were brought for slaughter from different animal farms of Siirt. Overall, 20 sheep which were determined to be healthy in physical examinations and had no pathological lesions in their internal organs were determined as the control group, while the remaining 20 sheep with cystic lesions in their lungs and livers which were protoscolex-positive formed the infected group. While no significant relevant difference between the K, Cl., Ca, Mg, GGT, ALT, ALB, TRIG. and CHOL levels of health and infected animals (1)>0.05), statistically meaningful changes in Na (p<0.01). TBIL (p<0.05), and TP and GLOB (p<0.001) levels and AST (p<0.001) activity were observed between the groups. As a result, it was determined that the changes in AST, total protein, globulin, and total bilirubin levels could be used as supportive laboratory analyzes in a determination of the prognosis of animals infected with Cystic Echinococcosus and of the physiopathologic alterations occurring in their livers.
Investigation of the Protective Effect of Kefir Against Isoproterenol Induced Myocardial Infarction in Rats
(Korean Soc Food Science Animal Resources, 2018) Mert, Handan; Yilmaz, Hikmet; Irak, Kivanc; Yildirim, Serkan; Mert, Nihat
This study aims to investigate the protective effects of kefir against myocardial infarction induced by isoproterenol (ISO). The rats were randomly divided into 4 groups, each group consisting of 8 rats. The control group, the kefir group (5 mL/kg/d kefir administered to rats as intra-gastric gavage for 60 d), the ISO group (100 mg/kg ISO was administered to rats, s.c. on 61. and 62. d), and kefir+ISO group (5 mL/kg/d kefir was administered to rats intra gastric gavage for 60 days prior to ISO, 100 mg/kg in two doses on day 61 and 62). 12 h after the last ISO dose, all rats were decapitated and their blood samples were collected. Cardiac tissue was reserved for histopathological examination. creatine kinase (CK), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), triglycerides, total cholesterol,very low density lipoprotein (VLDL), low density lipoprotein (LDL), high density lipoprotein (HDL) and glucose were measured by autoanalyzer, whole blood malondialdehyde (MDA), glutathione (GSH) and plasma advanced oxidation protein products (AOPP) levels were measured spectrophotometrically. It was determined that in the group of kefir+ISO, the levels of AST (p<0.001), CK (p<0.001), LDH (p<0.001), MDA (p<0.001) and AOPP (p<0.001) were decreased, while the GSH (p<0.05) increased, compared to ISO group. There were no significant changes in lipid profile and glucose levels between these two groups. In conclusion, by examining cardiac enzymes and histopathological changes in cardiac tissue, it can be concluded that the administration of kefir in myocardial infarction induced by ISO can protect the heart with its antioxidant characteristic and minimize the toxic damage created by ISO.
Molecular Identification and Phylogenetic Analysis of Babesia Ovis in Sheep in Siirt, Turkiye: Relationship With Some Oxidant/Antioxidant Parameters
(veterinarni A Farmaceuticka Univerzita Brno, 2024) Aslan Celik, Burcak; Kara, Murat; Celik, Ozgur Yasar; Irak, Kivanc; Selcuk, Muhammed Ahmed; Ercan, Kerem; Ayan, Adnan
The Babesia genus includes tick-borne haemoprotozoan parasites that infect a wide variety of vertebrate hosts, both domestic and wild, around the world. Babesia spp. cause oxidative stress by increasing the number of free radicals in erythrocytes. Among this genus, Babesia ovis causes babesiosis in sheep and goats, especially in tropical and subtropical regions, causing significant economic losses. In this study, we aimed to determine the presence and prevalence of B. ovis in sheep in Siirt province, T & uuml;rkiye, using molecular method and to evaluate some oxidant/ antioxidant parameters in infected sheep. The animal material used in this study consisted of a total of 500 sheep. DNA extraction, Polymerase Chain Reaction (PCR) amplification and sequence analysis of blood samples and Enzyme-Linked ImmunoSorbent Assay (ELISA) analysis of serum samples were performed. As a result of the study, 84 of the 500 samples (16.80%) examined were PCR positive. Advanced oxidation protein products (AOPP) and nitric oxide (NO) concentrations were found to be higher in sheep with babesiosis compared to the control group. Superoxide dismutase (SOD) activity was higher in the control group and catalase (CAT) activity was higher in infected sheep. As a result of this study, the presence of Babesia ovis was detected in all districts of Siirt province. It has been determined that infection caused by Babesia ovis in sheep causes oxidative stress as a result of increased nitric oxide and oxidized protein levels and this process may participate in the pathology of the disease.
Nephroprotective Effect of Ferulic Acid on Gentamicin-Induced Nephrotoxicity in Female Rats
(Taylor & Francis Ltd, 2022) Erseckin, Vasfiye; Mert, Handan; Irak, Kivanc; Yildirim, Serkan; Mert, Nihat
Ferulic acid is a kind of phenolic compound that can be found in various fruits and vegetables. This study aims to investigate the effect of ferulic acid on nephrotoxicity induced by gentamicin (GM). In this study, rats were separated into 4 groups such that each containing 8 randomly selected rats: Control group, Ferulic Acid (FA) group, Gentamicin (GM) group and Gentamicin + Ferulic acid (GM + FA) group. Blood samples were collected after 24 hours following the 8-day trial period, and kidneys were taken out for histopathological evaluation. Serum urea, creatinine, uric acid and LDH analyses were performed in autoanalyzer while Malondialdehyde (MDA), Advanced Oxidized Protein Products (AOPP), Glutathione (GSH), Superoxide dismutase (SOD), Catalase (CAT), Interleukin 6 (IL-6), Tumor Necrosis Factor-alpha (TNF-alpha) analyses were performed in ELISA, and kidney tissues were also examined histopathologically. Urea (p < .001), creatinine (p < .001), MDA (p < .01), AOPP (p < .001), IL-6 (p < .01) and TNF-alpha (p < .001) levels were found to be statistically and significantly lowered in GM + FA group when compared to GM group. As a result, ferulic acid has reduced the inflammation in nephrotoxicity induced by GM, causing decreased oxidative stress. In this study, anti-inflammatory features of ferulic acid have come to the forefront rather than the antioxidant features. It can be said that ferulic acid reduces nephrotoxic damage and has protective properties for kidneys.
Oxidant/Antioxidant Status, Pon1 and Ares Activities, Trace Element Levels, and Histological Alterations in Sheep With Cystic Echinococcosis
(Iranian Scientific Society Medical Entomology, 2018) Irak, Kivanc; Celik, Burcak Aslan; Karakoc, Zelal; Celik, Ozgur Yasar; Mert, Handan; Mert, Nihat; Kaya, Mustafa Oguzhan
Background: Total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI), nitric oxide (NO), zinc (Zn), copper (Cu) levels, paraoxonase (PON1), arylesterase (ARES) activities, and biochemical changes were studied on sheep with cystic echinococcosis. Methods: The materials were taken from 2-3 yr old sheep slaughtered in Van Province, Turkey in 2017. Before the slaughter, blood samples were collected from the healthy sheep, while various organs of animals were examined for hydatid cysts after the slaughter. Thirty sheep were protoscolex positive, hydatic group, while 30 sheep that did not have any pathological lesions in organ examinations were accepted as the control group. TOS levels, PON1 and ARES activities, and Zn levels were determined by commercial kits, while Cu levels were determined by atomic absorption spectrophotometer. The collected data were then statistically analyzed. Results: Serum TOS and OSI levels were significantly higher in sheep with cystic echinococcosis compared to the control group (P<0.001). TAS levels (P<0.01), PON1 and ARES activities, on the other hand, were significantly higher in control group compared to the cystic echinococcosis group (P<0.001). There were no significant differences in Zn, NO and Cu levels between the groups. Conclusion: PON1 and ARES activities increased in sheep infected with cyst hydatid. The decline of antioxidant reserves in the metabolism results in excessive amounts of free radicals, along with alterations of the normal histological structure of the cystic organ and changes in trace element metabolism.
Renal Protective Effect of Grape Seed Extract in Streptozotocin-Induced Diabetic Rats
(Parlar Scientific Publications (p S P), 2022) Sogutlu, Inci; Irak, Kivanc; Mert, Handan; Yildirim, Serkan; Mert, Nihat
It was aimed to investigate the effects of grape seed extract (GSE) on structural changes that occur in renal tissues of rats with streptozocin (STZ)-induced diabetes. Rats were randomly divided into four groups (Control, Diabetes Mellitus (DM), GSE, Diabetes Mellitus+ GSE (DM + GSE) groups) each containing 8 rats. When GSE was given, the values of serum creatinine, BUN and uric acid in diabetics were decreased. Kidneys of diabetic group were found to have mild interstitial nephritis, tubular dilatation, hydropic degeneration, clotting necrosis in the tubular epithelium, dilatation in glomerulus and Bowman capsule, and hyperemia in glomerular capillaries. In the DM + GSE group, the glomeruli were normal, but hydropic degeneration was observed in some of the tubule epithelium. Only a few 8-OhDG positive cells were detected in tubule epithelium in the diabetes + grape seed group. The administration of GSE to diabetic patients helped to heal the deteriorated of kidney condition and DNA damage.
Retinol, Α-Tocopherol and Vitamin D3 in White Muscle Disease
(Polish Soc veterinary Sciences Editorial office, 2018) Mert, Handan; Yildirim, Serkan; Yoruk, Ibrahim Hakki; Irak, Kivanc; Comba, Bahat; Mert, Nihat; Comba, Arzu
Vitamins are essential for the health of all living organisms. Vitamins E, A, D and K are known as fatsoluble vitamins, and deprivation of vitamin E causes various disorders, especially in the reproduction and cardiovascular systems and in muscle functions. Vitamin A, on the other hand, has roles in various biological functions - like eyesight - and the growth, reproduction and differentiation of epithelial cells. Vitamin A deficiency leads to the keratinization of the epithelium, and disorders related to the metaplasies of the genital and genitourinary systems. Conversely, vitamin D is defined as a pro-hormone and is responsible for Cahomeostasis, and thus indirectly affects the bone metabolism, bone structure, and cellular and neural functions of Ca. White Muscle Disease (WMD) can occur in newborn lambs, but is more commonly seen in lambs of up to 3 months of age. In this study, 30 lambs of 3 to 50-days-old from different flocks diagnosed with White Muscle Disease (WMD) were selected as research material, while the control group consisted of 8 healthy lambs. With the aim of clarifying the cause of WMD, serum fat-soluble vitamins, retinol, alpha-tocopherol and vitamin D3 levels were determined in 16 lambs. Gluteal and heart musclet issue samples also were taken from 30 lambs with WMD. The vitamin levels of the samples were analysed by HPLC. The levels of serum alpha-tocopherol, retinols, and vitamin D3 were foundto be low in the diseased animals, but only retinol (p < 0.001) and alpha-tocopherol (p < 0.001) level differences were statistically relevant. Macroscopically, Zenker's necrosis was determined in the heart muscles of 17 lambs, and in the gluteal and chest muscles of 6 lambs. 7 lambs displayed necrosis in both their heart and in gluteal muscles. The samples were analyzed microscopically to reach similar findings: swollen homogeneous pink muscles, pycnotic nuclei, and hyperaemic and haemorrhagic blood vessels in gluteal, chest and heart muscles. Hyaline degeneration and Zenker's necrosis, dystrophic regions in necrotic areas, cc was detected as a severe disease in lambs at an early stage of life with advanced degeneration in different muscle tissues. Deficiency of fat-soluble vitamins was also detected in the sick animals. Control group lambs had higher levels of alpha-tocopherol and retinol (p < 0.001) compared to the sick lambs.