Browsing by Author "Isler, S."

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Gundelia Rosea Seed: Evaluation of Biopharmaceutical Potential and Bioactive Composition
(Elsevier, 2019) Dalar, A.; Zengin, G.; Mukemre, M.; Bengu, A. S.; Isler, S.
Gundelia species are among significant key medicinal plants extensively utilized in folk medicine of Middle Eastern countries. This study focused on researching the biopharmaceutical potency and bioactive compounds of Gundelia rosea seed. Hereby, traditional knowledge-based preparing methods (infusion and decoction) and ethanol-based lyophilized extracts obtained from Gundelia rosea seeds were assessed for (i) antioxidant capacities, (ii) enzyme inhibitory activities, (iii) HPLC-MS/MS and (iv) GC-MS studies. Phytochemical analysis revealed that ethanol extract which primarily compromised of mainly phenolics (4-Caffeoylquinic acid and luteolin hexoside) and several fatty acids (palmitic, stearic, oleic and linoleic acids), was superior to those of infusion and decoction extracts. Antioxidant activities findings revealed that ethanol extract contained a high level of total phenolics (55.3 mg Gallic acid Eq./g extract) and had high capacities of reducing (1683 mu mol Fe2+ and 214.1 mg Trolox Eq./g extract for FRAP and CUPRAC respectively) and radical scavenging (ORAC: 2241.9 mu mol, DPPH: 91.7 mg, ABTS: 141.2 mg Trolox Eq./g extract) and total antioxidant (Phosphomolybdenum: 1.39 mmol Trolox Eq./g extract) properties. The suppressive abilities of the extracts against selected isolated enzymes revealed that ethanol extract had pronounced levels of inhibitory activities against AChE (4.3 mg Galanthamine Eq.), BChE (3.4 mg Galanthamine Eq.), tyrosinase (120 mg Kojic acid Eq.), amylase (0.61 mmol Acarbose Eq.), glucosidase (11.91 mmol Acarbose Eq.) and lipase (53.4 mu mol Orlistat Eq.) per gram extract. Findings obtained within this study confirmed the traditional utilization of Gundelia rosea and suggest its potential as a novel candidate of biopharmaceutical agents for public health problems. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved.
In Vitro Symbiotic Germination Potentials of Some Anacamptis, Dactylorhiza, Orchis and Ophrys Terrestrial Orchid Species
(Corvinus Univ Budapest, 2018) Cig, A.; Durak, Demirer E.; Isler, S.
Germination and propagation of terrestrial orchids is highly challenging due to their small size seeds and absence of endosperms. Some orchid species are extremely dependent on fungi for germination of their seeds. Although germination occurs symbiotically between the seed and the fungus, it is not coincidental that fungus species are specific to orchid species. Aim of this study is to show the impact of the fungi, isolated from the tubers of ten naturally grown species of orchids, collected from mountainous area of Van province, Turkey, on in vitro symbiotic germination of the seeds. The collected orchids species were Dactylorhiza romana (Seb.) Soo subsp. georgica (Klinge) Soo ex Renz & Taub., Orchis pinetorum Boissier & Kotschy, Orchis spitzelii Saut. ex W.D.J. Koch, Orchis coriophora L., Orchis collina Banks & Solander, Orchis anatolica Boiss., Orchis simia Lamarck, Ophrys straussii H. Fleischmann & Bornmuller, Dactylorhiza umbrosa (Kar. & Kir.) Nevski, Anacamptis pyramidalis (L.) L. C. Rich. In the study from orchid tubers, Rhizoctonia sp., Aspergillus sp., Alternaria sp., Penicillium sp., Trichoderma sp. and Fusarium sp. fungi species are isolated. Firstly, orchid seeds and fungal isolates placed in oat culture medium were transferred to Van Waes & Debergh culture medium in subculture. The highest germination rates obtained in the seeds are 71.19% in Dactylorhiza romana subsp. georgica species with Rhizoctonia sp.; 78.26% in Orchis pinetorum species with Penicillium sp.; 27.77% in Orchis spitzelii species with Fusarium sp.; 83.07% in Orchis coriophora species with Rhizoctonia sp.; 75.00% in Orchis collina species with Fusarium sp.; 38.88% in Orchis anatolica species with Alternaria sp.; 73.91% in Orchis simia species with Fusarium sp.; 91.60% in Ophrys straussii species with Fusarium sp.; 93.75% in Dactylorhiza umbrosa species with Fusarium sp. and 56.00% in Anacamptis pyramidalis species with Rhizoctonia sp. At the end of the study, it was understood that the fungi isolated from their own or other tubers had different effects on the germination of each orchid species.