Browsing by Author "Isler, Sinan"

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Molecular Phylogenetics of Some Orchis Species (Orchidaceae) Native To Turkey Using Nuclear and Chloroplast Dna Sequences
(Gazi Univ, 2017) Dizkirici, Ayten; Isler, Sinan; Yigit, Oktay
Phylogenetic relationships among nine Turkish Orchis species were inferred from variation in the internal transcribed spacer and maturase Kinase regions. Foreign sequences were also retrieved from NCBI to increase interspecific sampling. The topology of ITS tree was broadly congruent to that of matK tree. The trees showed two major clades; the first one included species from Orchis and Neotinea subgenera and the second included species of Anacamptis subgenus. Phylogenetic separation of some species found in Neotinea and Anacamptis subgenera were previously reported, and the current study also indicated that moving these species into Neotinea and Anacamptis genera could be reasonable.
Morphological and Chemical Characteristics of Fritillaria Species: Species Differentiation Through Morphometric Measurements and Gc-Ms Analysis
(Cambridge Univ Press, 2025) Senturk, Bengisu; Aytar, Erdi Can; Durmaz, Alper; Isler, Sinan; Deniz, Ismail Gokhan; Kompe, Yasemin Ozdener
This study examines the morphological and chemical characteristics of seeds from five Fritillaria species: Fritillaria pinardii, Fritillaria pontica, Fritillaria kittaniae, Fritillaria imperialis and Fritillaria alfredae Post subsp. glaucoviridis (Turrill) Rix. Morphological measurements included total length, total width, embryo length, embryo width, seed left-wing coverage width, seed right-wing coverage width and the distance between crossing points. These measurements revealed significant differences among the species. For example, F. imperialis exhibited the longest seeds and the largest embryos, while F. alfredae Post subsp. glaucoviridis had the smallest dimensions. Chemical analyses were conducted using gas chromatography-mass spectrometry, identifying various significant compounds across the species. High proportions of 2,2-dimethoxybutane were found in the seed samples. In F. pontica, compounds such as 2,2-dimethoxybutane (66.33%) and 1,1-dipropoxypropane (13.24%) were prevalent. Fritillaria kittaniae seeds showed high levels of benzene, 1,1 '-(3,3-dimethyl-1-butenylidene) bis- (25.57%) and cyclohexene, 3-methyl-6-(1-methylethylidene)- (6.89%). In F. imperialis, significant compounds included 1,3-dioxolane-4-methanol, 2-ethyl-2-methyl (9.73%) and dodecane (5.73%). Fritillaria pinardii had notable amounts of 3,6-dimethyloctane (4.81%), while F. alfredae subsp. glaucoviridis contained 2-methoxyethyl(trimethyl)silane (13.21%). Principal component analysis and cluster analysis revealed clear groupings based on morphological and chemical similarities. Fritillaria pinardii, F. pontica and F. kittaniae formed a cluster due to their similar morphological and chemical characteristics, whereas F. imperialis and F. alfredae subsp. glaucoviridis formed a distinct group. These findings provide valuable insights into the identification and classification of Fritillaria species. Integrating morphological and chemical data can enhance the accurate identification of these species. This study contributes to understanding the natural diversity of Fritillaria species and has implications for ecological studies.