Browsing by Author "Izgu, Tolga"

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Integrating in Vitro Propagation and Machine Learning Modeling for Efficient Shoot and Root Development in Aronia Melanocarpa
(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Yaman, Mehmet; Palaz, Esra Bulunuz; Isak, Musab A.; Demi̇Rel, Serap; Izgu, Tolga; Adali, Sümeyye; Demirel, Fatih
Aronia melanocarpa (black chokeberry) is a medicinally valuable small fruit species, yet its commercial propagation remains limited by low rooting and genotype-specific responses. This study developed an efficient, callus-free micropropagation and rooting protocol using a Shrub Plant Medium (SPM) supplemented with 5 mg/L BAP in large 660 mL jars, which yielded up to 27 shoots per explant. Optimal rooting (100%) was achieved with 0.5 mg/L NAA + 0.25 mg/L IBA in half-strength SPM. In the second phase, supervised machine learning models, including Random Forest (RF), XGBoost, Gaussian Process (GP), and Multilayer Perceptron (MLP), were employed to predict morphogenic traits based on culture conditions. XGBoost and RF outperformed other models, achieving R2 values exceeding 0.95 for key variables such as shoot number and root length. These results demonstrate that data-driven modeling can enhance protocol precision and reduce experimental workload in plant tissue culture. The study also highlights the potential for combining physiological understanding with artificial intelligence to streamline future in vitro applications in woody species. © 2025 Elsevier B.V., All rights reserved.
Synthetic Seed Production of Flower Bulbs
(Springer Nature Switzerland Ag, 2019) Kocak, Metin; Sevindik, Basar; Izgu, Tolga; Tutuncu, Mehmet; Mendi, Yesim Yalcin
Flower bulbs are perennial or annual plants with underground structures such as bulb, corn, tuber, and rhizomes. These plants have economic value especially in ornamental plant sector as cut flower, potted flower, and outdoor plants. Most of these plants have garish flower, and many of them are monocotyledon. Cyclamen, Tulipa, Lilium, Narcissus, Gladiolus, Hyacinthus, Crocus, Iris, Allium, Alstroemeria, Anemone, Orchis, Rhododendron, Freesia, Hippeastrum, Muscari, Ornithogalum, Ranunculus, and Zantedeschia are the most important geophytes that are commercially used in the world. These plants can be propagated using conventional and tissue culture techniques. Synthetic seed production is one of these techniques. Synthetic seed, namely, artificial seed, is described as artificially encapsulated plant tissues and somatic embryos with alginate hydrogel. Synthetic seed technology has significant effect on the conservation of the plant tissues and sustainability of the plants. Recently, conservation of the plant species studies significantly increased, and artificial seed method was used as the most common process to conserve important species. In this chapter, oldest and newest synthetic seed production researches were discussed and presented chronologically.