Browsing by Author "Kömüroğlu, A.U."

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The Effect of Infliximab on Oxidative Stress in Ovarian Tissue in the Rat of Ovarian Hypersrimulation Syndrome
(Yuzuncu Yil Universitesi Tip Fakultesi, 2021) Dirik, D.; Kömüroğlu, A.U.
Ovarian hyperstimulation syndrome (OHSS) is a complication that occurs during assisted reproductive techniques. In this study, our aim is to study the effect of Infliximab (IFX) on oxidative stress in ovarian tissue in a rat model of ovarian hyperstimulation syndrome. A total of 32 immature female rats were divided into four groups rando mly: Control, OHSS, OHSS+IFX, and IFX group. OHSS and OHSS+IFX groups were administered 30 IU pregnant mare serum gonadotropin/day on days 22-25 of life and 30 IU hCG on day 26. Plus, intraperitoneal IFX was administered half an hour before hCG administration to the OHSS+IFX group. On the 26th day, solely 7 mg/kg IFX was administered to the IFX group. 48 hours after administration of hCG administration, the rats were sacrificed and their ovarian tissues were sampled. The levels of MDA, AOPP, TSG as well as catalase activity were measured in these ovarian tissues. Ovarian tissue MDA and AOPP levels of the OHSS group were determined to be significantly higher compared to the control and OHSS-IFX groups. Ovarian tissue catalase activity and TSG level of the control group was significantly higher compared to the OHSS and OHSS-IFX groups. It was determined that although ovarian tissue catalase activity and TSG level in the OHSS-IFX group were higher compared to the OHSS group but it was not significant. The results revealed that IFX could prevent oxidative stress in ovarian tissue induced by O HSS. These effects may be mediated by the anti-inflammatory and antioxidant properties of IFX. © 2021, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Effects of Sedation Doses of Propofol and Midazolam on Levels of Ngal, Cystatin-C, Kim-1 in Rats
(Yuzuncu Yil Universitesi Tip Fakultesi, 2022) Soyalp, C.; Kömüroğlu, A.U.; Yuzkat, N.; Basbugan, Y.; Tunçdemir, Y.E.
Limited data are available regarding the impact of their sedation doses midazolam and propofol on early biomarkers of acute kidney injury (AKI). This study aimed to investigate the effects of sedation doses propofol and midazolam on early biomarkers such as neutrophil gelatinase-associated lipocalin (NGAL), cystatin C (CyC) and kidney injury molecule 1 (KIM-1) of AKI. A total of 24 Wistar albino rats were separated into three groups (n = 8 per group): a control group (intraperitoneal injecti on [IP] saline injection once daily for 7 days), a propofol group (IP injection of 2.5 mg/kg propofol once daily for 7 days), and a midazolam group (IP injection of 5 mg/kg midazolam once daily for 7 days). For each group, urinalysis (for urea, creatinine, total protein, NGAL, CyC, and KIM-1) was performed on Day 0 and Day 7; serum analysis (for urea, creatinine, total protein, albumin globulin, ALT, AST, NGAL, CyC, and KIM-1) was performed on day 7. No significant difference was noted between control, propofol and midazolam groups in terms of Day 7 serum KIM-1, CyC, and NGAL levels and Day 0 and Day 7 urinalysis findings (KIM-1, CyC, NGAL, urea, and creatinine levels). DISCUSSION AND CONCLUSION: The findings revealed a similar safety profile for seven-day propofol and midazolam administration in rats in terms of the traditional (creatinine, urea) and early biomarkers (NGAL, CyC, KIM-1) of AKI. © 2022, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Protective Effect of Subsieve Grape Extract (Vitis Vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats
(Ataturk Universitesi, 2023) Koşal, V.; Avci, A.; Bingöl, N.T.; Keleş, Ö.F.; Kömüroğlu, A.U.; Okulmuş, Ç.
Heat stress (HS) has negative effects on reproductive parameters in male animals. This study was designed to investigate the protective effects of Vitis vinifera L., which has a natural antioxi-dant property, on reproductive parameters, testicular oxidative stress, reproductive hormones, and testicular histopathology in male rats treated with HS. Fifty male rats were divided into 5 different groups (control, HS, HS + 100, HS + 200, and HS + 300). It was determined that subsieve V. vinifera extract given at different doses eliminated the negative effects of HS administration on reproductive parameters. It was determined that V. vinifera increased sperm motility and density and decreased the rate of abnormal sperm regardless of dose (P <.001). It was determined that increased oxidative stress and decreased luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone levels as a result of HS administration improved in the extract-treated groups (P <.05), and FSH and testosterone levels increased in the HS + 200 and HS + 300 groups compared to the control group (P <.001). Histopathologically, it was determined that the degen-eration, edema, and congestion caused by HS disappeared due to the extract administration. As a result of this study, the protective effect of subsieve V. vinifera extract on HS on male reproductive parameters was revealed. © Author(s).