Browsing by Author "Kandemir, Ozlem"

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Correlation Between Intrahepatic Hepatitis B Virus Cccdna Levels and Other Activity Markers in Patients With Hbeag-Negative Chronic Hepatitis B Infection
(Lippincott Williams & Wilkins, 2011) Guner, Rahmet; Karahocagil, Mustafa; Buyukberber, Mehmet; Kandemir, Ozlem; Ural, Onur; Usluer, Gaye; Tasyaran, Mehmet A.
Objective The aim of this study was to demonstrate the relation between intrahepatic (IH) hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) levels and the other HBV replicative intermediates and hepatocyte expression of HBV antigens. Patients and methods Patients with hepatitis B surface antigen (HBsAg) positivity, hepatitis B early antigen negativity, serum HBV DNA levels 10(4) copies/ml or more, and constantly or intermittently increased alanine aminotransferase levels were included. Results Fifty-nine patients were included. There was a good correlation between the levels of IH HBV cccDNA and serum HBV DNA (P<0.001). Serum HBsAg levels were weakly correlated with IH HBV cccDNA levels and moderately correlated with serum HBV DNA (r=0.322, P=0.017; r=0.489, P=0.001, respectively). There were no significant correlation between serum HBsAg level and histologic activity index groups (P=0.691), but stage 0, 1, and greater than 2 fibrosis groups were positively correlated with serum HBsAg levels (P=0.019). IH cccDNA and serum HBV DNA were significantly different in hepatitis B core antigen staining groups (P=0.008 and <0.001, respectively) but there was no significant correlation between HBsAg staining groups and HBV replication markers. There was a weak correlation between serum HBsAg levels and IH HBsAg and hepatitis B core antigen levels (r=0.333, P=0.012; r=0.366, P=0.006, respectively). In multivariate analysis, alanine aminotransferase, age, fibrosis stage, and serum HBsAg quantitation were the most important factors predicting IH HBV cccDNA level. Conclusion Histopathologic damage, serum HBV DNA levels, and IH HBV replication markers have a more complex and dynamic process. However, both serum and IH HBV replication markers provide important knowledge about the activity of the disease. Eur J Gastroenterol Hepatol 23:1185-1191 (C) 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins.
Investigation of the Association Between Chronic Hepatitis B and C Infection and Interleukin 2 (-330) Gene Polymorphism
(Gazi Univ, Fac Med, 2016) Borekci, Gulay; Talin, Serap; Kandemir, Ozlem; Celik, Sevim Karakas; Berkoz, Mehmet; Aras, Nurcan
Objective: Cytokines has an important role in the immunopathogenesis of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections. Interleukin-2 (IL-2) secreted by Th1 cells which plays an important role in regulating both in activation of the immune system and homeostasis, is a cytokine having a wide spectrum of effects on the immune system. Although there are many studies investigating the relationship between IL-2-330 gene polymorphism and diseases, a few studied was found to investigate the role in the immunopathogenesis of HBV and HCV infections of this cytokine polymorphisms. This study was aimed to determine the relationship between IL-2-330 gene polymorphisms and chronic hepatitis B and C infections. Methods: A total of 139 patients with chronic hepatitis B, 101 patients with hepatitis C and 87 healthy subjects as control groups were included into this study. Approximately 2 ml of blood from patients and control groups were taken into tubes containing EDTA, and genomic DNA was isolated using DNA isolation kit. Single nucleotide polypmorphsim from the obtained DNAs was investigated using the polymerase chain reaction-confronting two-pair primers (PCR-CTPP) methods. Results: The genotype frequencies of IL-2-330 TT, GT, GG were detected as 23.7%, 53.2%, 23% in patients with chronic hepatitis B and 27.6%, 50.6%, 21.8% in control groups, respectively (p>0.05). The frequencies of TT, GT, GG genotypes were found to be 34.7%, 56.4%, 8.9% in patients with chronic hepatitis C and 27.6%, 50.6%, 21.8% in control group, respectively. GG genotype frequency was significantly lower in patient groups with hepatitis C compared with the control group (p<0.05). The frequency of T allele was 50.4% in patients with chronic hepatitis B and 52.9% in control groups. The frequency of G allele in patients with chronic hepatitis B was 49.6% and 47.1% in control groups ((p>0.05). The frequencies of T and G alleles were found to be 69.4%, 30.6% in patients with chronic hepatitis C and 52.9%, 47.1% in control groups, respectively (p<0.05). Conclusion: In our study while there was no statistically significant relationship between chronic hepatitis B and IL-2-330 gene polymorphisms, significant association was found between GG genotype and chronic hepatitis C. According to our findings the GG genotype in the-330 position of IL-2 gene may be preventive effect in chronicy of hepatitis C in Turkish population, however, further research can contribute to clarify the issue.
Investigation of the Association Between Chronic Hepatitis B and C Infections and Tnf-Α( Gene Polymorphism
(Ankara Microbiology Soc, 2016) Borekci, Gulay; Aras, Nurcan; Kandemir, Ozlem; Yalin, Serap; Celik, Sevim Karakas; Berkoz, Mehmet
Cytokines and genetic factors play important roles in the pathogenesis of chronic hepatitis B (CHB) and chronic hepatitis C (CHC) infections. Variations in cytokine genes may effect the gene expression and may lead to changes in the clinical manifestations of diseases. One of the single nucleotide polymorphisms in the promoter region of tumor necrosis factor-alpha (TNF-alpha) gene is the polymorphism at -308. position which was investigated in many studies by means of its relationship between CHB and CHC infections, however their results are incompatible. Furthermore, there is no sufficient data on this subject in our country. This study was aimed to determine the relationship between TNF-a(-308) gene polymorphism with CHB and CHC infections. A total of 271 patients with chronic hepatitis and 181 healthy subjects were included in the study. Of them 167 were CHB cases (67 female, 100 male; age range 18-74 years, mean age: 40.23 +/- 13.09) and 95 controls for CHB group (46 female, 49 male; mean age: 36.41 +/- 15.0 years), while 104 were CHC cases (63 female, 41 male; age range: 25-79 years, mean age: 52.8 +/- 12.6) and 86 controls for CHC group (41 female, 45 male; mean age: 36.4 +/- 14.9 years). After the isolation of genomic DNA from blood samples of the patient and control groups, TNF-alpha(-308)G/A (rs 1800629) polymorphism was investigated by using the real-time polymerase chain reaction from the obtained DNAs. Among CHB group, TNF-alpha(-308) GG, GA, AA genotypes were detected in 126 (75.4%), 38 (22.8%) and 3 (1.8%) of the patients, respectively, while these numbers were 84 (88.4%), 11 (11.6%) and 0 (0%) in control group, respectively. Among CHC group, TNF-a(-308) GG, GA, AA genotypes were detected in 37 (35.6%), 28 (26.9%) and 39 (37.5%) of the patients, respectively, while these numbers were 38 (44.2%), 8 (9.3%) and 40 (46.5%) in control group, respectively. The frequency of GA genotype was significantly higher in both patient groups compared to the control groups (p=0.024 for CHB and p= 0.006 for CHC). When the distribution of allele frequencies of TNF-alpha(-308)G/A polymorphism was evaluated in the patients and control groups, it was noted that G allele was found to be high in CHB patients comparing with controls (94.2% vs 86.8%), however A allele was identified to be lower than controls (5.8% vs 13.2%) (p= 0.008). In contrast, there was no significant difference in terms of allele frequency compared with CHC patients and the control group (p= 0.969). In conclusion, our data in accordance with the results of many studies in literature, determined that TNF-alpha(-308) polymorphisms can influence the chronicity of hepatitis B and C infections. Further studies on this subject would contribute to the elucidation of the molecular mechanisms of chronic hepatitis B and C diseases.