Browsing by Author "Karaca, T."

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Age Related Distribution of Mast Cells in the Trachea and Lung of Chicken
(indian veterinary Journal, 2006) Karaca, T.; Yoruk, M.; Simsek, N.
Age-Related Changes in the Number of Mast Cells in the Avian Lymphoid Organs
(Wiley-blackwell Publishing, inc, 2006) Karaca, T.; Yoruk, M.; Uslu, S.
The distribution of mast cells (MCs) was studied in the lymphoid organs (thymus, bursa of Fabricius and spleen) of 0-, 7-, 21-, 30- and 120-day-old chickens, using light microscopic histochemical techniques. Tissues samples were obtained under deep anaesthesia from animals in five groups. Tissues were fixed in Mota's fixative (basic lead acetate) for 24 h and embedded in paraffin. Six-micrometre-thick sections were stained with toluidine blue in 0.5% aqueous solution at pH 1.0 for 5 min and Alcian blue/Safranine at pH 1.42 for 30 min. MCs were found in the organs, mostly associated with sinuses and blood vessels. A large increase in MCs was observed in both thymus and spleen of 21-day-old chickens compared with 0-, 7-, 30- and 120-day-old chickens. However, in the bursa of Fabricius, numbers of MCs were significantly higher in the 7-day-old group compared with other age groups. Safranine-positive MCs were not observed in all organs and age groups. These results showed age-related changes in the number of MCs in avian lymphoid tissues.
Distribution and Heterogeneity of Mast Cells in Female Reproductive Tract and Ovary on Different Days of the Oestrus Cycle in Angora Goats
(Wiley-blackwell, 2008) Karaca, T.; Arikan, S.; Kalender, H.; Yoruk, M.
The physiological distribution of mast cells (MCs) in the reproductive tract and ovary of 12 Angora goats was determined using light microscopic histochemical techniques. Uterus (corpus uteri and cornu uteri), uterine cervix, uterine tubes (isthmus and ampulla) and ovary samples were obtained by laparatomy from groups of animals during metoestrus, dioestrus and proestrus (days 5, 10 and 16 of the oestrous cycle). Tissues were fixed in Mota's fixative (basic lead acetate) for 48 h and embedded in paraffin. Six-micrometre-thick sections were stained with toluidine blue in 1% aqueous solution at pH 1.0 for 5 min and alcian blue-Safranin at pH 1.0 for 30 min. MCs were generally associated with blood vessels in all reproductive organs. In the uterus, they were concentrated mainly in the close of the uterine gland and deep stroma in the endometrium. Higher MC numbers were observed by toluidine blue staining in the uterus, uterine cervix and uterine tubes on days 10 (corpus uterine: 4.7 +/- 3.8 and cornu uterine: 4.9 +/- 3.5) and 16 (corpus uterine: 5.9 +/- 4.5 and cornu uterine: 5.4 +/- 2.4) of the oestrous cycle compared with day 5 (p < 0.05). Mast cells were not observed in the follicles, the corpus luteum and the underside of the surface epithelium of the ovarian cortex, but were observed in the interstitial cortical stroma and the ovarian medulla. In the ovary, MC numbers were significantly higher on day 16 of the oestrous cycle (cortex: 3.4 +/- 2.4 and medulla: 5.7 +/- 4.5, p < 0.05). Safranin-positive connective tissue MCs were not observed in the uterine tube on any occasion. These results indicate oestrous cycle-related changes in the number and location of MCs in goat reproductive organs.
Distribution and Quantitative Patterns of Mast Cells in Ovary and Uterus of Rat
(Univ Austral Chile, Fac Ciencias veterinarias, 2007) Karaca, T.; Yoeruek, M.; Uslu, S.
The aim of this study was to evaluate the distribution of mast cells in the ovary and uterus of rats during their oestrous cycle. Fourty female Wistar Albino rats, 10-12 weeks old, were used. Ovary and uterus tissues were fixed in Mota's fixative for 48 It and embedded in paraffin. Sections of 6 mu m thickness were cut and stained with toluidine blue (1% aqueous solution) and Alcian blue-safranin (pH: 1.0, buffered by using 0.1 N HCl. In the ovary, the mast cells were mainly present in the tunica albuginea, in the interstitial areas between follicles or corpora lutea and in the vicinity of blood vessels in the medulla. The number of mast cells in the ovarian medulla and cortex and the uterine endometrium and myometrium were highest in the oestrous. metaoestrous, oestrous and metaoestrous phases, respectively. The number of mast cells was higher in the ovarian medulla (7.4 +/- 0.52) and cortex (2.1 +/- 030) during oestrus rather than in other phases of the oestrous cycle (P < 0.05), with the highest number in the endometrium during the oestrous (6.8 +/- 0.45) and metaoestrous (6.2 +/- 0.56) and in the myometrium (7.1 +/- 0.63 for oestrous, 7.5 +/- 0.33 for metaoestrous) of uterus compared to other phases of the cycle (P < 0.05). Safranin-stained mast cells were numerous in all tissues during all stages of the oestrous cycle except the proestrous. Mast cells were safranin positive in the myometrium only during proestrous. It was concluded that some physiological changes could be responsible for the variation in the distribution of mast cells in the ovarian and uterine tissues of the rat during the oestrus cycle.
Distribution and Quantitative Patterns of T Lymphocytes in the Female Reproductive Tract and Ovary Throughout the Oestrus Cycle of Angora Goats
(2007) Karaca, T.; Arikan, S.; Kalender, H.; Yoruk, M.
The present study was designed to evaluate the distribution of alpha-naphthyl acetate esterase (ANAE) positive T lymphocytes in the female reproductive tract and ovary throughout the estrous cycle of Angora Goats. Tissue samples were collected on days 5, 10 and 16 of the oestrus cycle and then fixed in formol-sucrose fixative (pH 6.8) and stored for 22 hours at +4°C. The samples were then additionally fixed in Holtz' solution under the same conditions they were in the first fixation. Cryostat sections of 8 μm thickness were stained for alpha naphthly acetate esterase activity at pH 6.4. The ANAE positive T lymphocytes were mainly located in the epithelium, lamina propria and around blood vessels in other region of connective tissues. Density of ANAE positive T lymphocytes in the ovarian and uterine tissues was highest on day 10 and 16 respectively. It was concluded that the estrous cycle may have been responsible for variations in the distribution of ANAE positive T lymphocytes in goat ovarian and reproductive tract tissues.
The Effect of Royal Jelly on Cd3+, Cd5+, Cd45+ T-Cell and Cd68+ Cell Distribution in the Colon of Rats With Acetic Acid-Induced Colitis
(Elsevier Espana Slu, 2012) Karaca, T.; Simsek, N.; Uslu, S.; Kalkan, Y.; Can, I.; Kara, A.; Yoruk, M.
Background: Traditional medicines and health supplements have historically been used to treat many illnesses but most of them have not been evaluated objectively to prove their efficacy. We have been investigating the effects of royal jelly (RJ) supplements on acetic acid-induced colitis on the distribution of CD3(+), CD5(+), CD45(+) T-cell and CD68(+) cells in rats. Methods: The rats were divided into four equal groups: control group, royal jelly-treated (RJ - 150 mg kg(-1) body weight), acetic acid-treated (colitis) and acetic acid-treated (colitis) + royal jelly (CRJ - 150 mg kg(-1) body weight). Colitis was induced by intracolonic instillation of 4% acetic acid; the control group received physiological saline (10 mL kg(-1)). Colon samples were obtained under deep anaesthesia from animals in four groups. Tissues were fixed in 10% formalin neutral buffer solution for 24 h and embedded in paraffin. Results: The proliferative response of CD3(+) and CD45(+) T cells stimulated with colitis was affected by colitis treated with RJ. No differences were found in CD5(+) T cells and CD68(+) macrophages in the colitis treated with RJ. Conclusions: This study has shown that RJ has anti-inflammatory and cell regeneration effect in the colon of rats with acetic acid induced colitis. (c) 2011 SEICAP. Published by Elsevier Espana, S.L. All rights reserved.
Effect of Royal Jelly on Experimental Colitis Induced by Acetic Acid and Alteration of Mast Cell Distribution in the Colon of Rats
(Pagepress Publ, 2010) Karaca, T.; Bayiroglu, F.; Yoruk, M.; Kaya, M. S.; Uslu, S.; Comba, B.; Mis, L.
This study investigated the effects of royal jelly (RI) on acetic acid-induced colitis in rats. Twenty adult female Wistar albino rats were divided into four treatment groups of 5 animals each, including a control group (Group I); Group II was treated orally with RJ (150 mg kg(-1) body weight); Group III had acetic acid-induced colitis; and Group IV had acetic acid-induced colitis treated orally with RJ (150 mg kg(-1) body weight) for 4 weeks. Colitis was induced by intracolonic instillation of 4% acetic acid; the control group received physiological saline (10 mL kg(-1)). Colon samples were obtained under deep anaesthesia from animals in all groups. Tissues were fixed in 10% formalin neutral buffer solution for 24 h and embedded in paraffin. Six-micrometre-thick sections were stained with Mallory's triple stain and toluidine blue in 1% aqueous solution at pH 1.0 for 5 min (for Mast Cells). RI was shown to protect the colonic mucosa against the injurious effect of acetic acid. Colitis (colonic damage) was confirmed histomorphometrically as significant increases in the number of mast cells (MC) and colonic erosions in rats with acetic acid-induced colitis. The RI treatment significantly decreased the number of MC and reduced the area of colonic erosion in the colon of RJ-treated rats compared with rats with untreated colitis. The results suggest that oral treatment with RI could be used to treat colitis.
Effects of Photoperiod on Number of Mast Cells in Lymphoid Organs of the Japanese Quail (Coturnix Coturnix Japonica)
(2010) Karaca, T.; Ari, H.H.; Yoruk, M.; Cinaroglu, S.
The distribution of Mast Cells (MCs) was studied in the lymphoid organs of 7, 14, 21 and 30 days old quails (Coturnix coturnix Japonica) kept in different photoperiods using light microscopy histochemical techniques. The distribution of MCs was determined in different age groups housed in continuous light (23L: ID) or in a light-dark regimen (18L: 6D). Tissue samples were obtained under deep anesthesia from birds in the four age groups, fixed in Mota's fixative (basic lead acetate) for 24 h and embedded in paraffin. Six micrometre-thick sections were stained with 0.5% toluidine blue and the number of MCs counted under the microscope. The numbers of MCs were significantly different between both age and light treatment groups. The number of mast cells was significantly lower (p<0.05) in birds exposed to continuous light (23L: ID) than in birds exposed to a shorter light period (18L: 6D). Photoperiod was concluded to influence mast cell numbers in the lymphoid organs of the Japanese quail and thus the immune response of the birds. © Medwell Journals, 2010.
Effects of the Spirulina Platensis and Panax Ginseng Oral Supplementation on Peripheral Blood Cells in Rats
(Ecole Nationale veterinaire Toulouse, 2007) Simsek, N.; Karadeniz, A.; Karaca, T.
The effects of Spirulina platensis and Panax ginseng dietary supplementations were analysed in 30 female adult Wistar albino rats divided in 3 equal groups. In the first and second groups, animals were treated with Spirulina platensis (300 mg/kg/day) and with Panax ginseng (400 mg/kg/day) respectively in drinking water for 30 days, whereas the third group served as control. Red Blood Cell (RBC) and White Blood Cell (WBC) counts, numeration of leukocyte types, Packed Cell Volume (PCV) and haemoglobin concentrations were determined by haemocytometric methods on blood samples collected on days 0, 15 and 30, and Mean Globular Volumes (MGV) and Mean Corpuscular Haemoglobin Concentrations (MCHC) were calculated. Populations of B and T lymphocytes were counted by the proportional to naphthyl acetate esterase (ANAE) staining method. Spirulina platensis and Panax ginseng treatments markedly stimulated the erythrocyte formation and the haemoglobin synthesis on day 30 and small erythrocytes (microcytosis) greatly loaded with haemoglobin (increases of MCHC) were obtained especially with Panax ginseng. Dramatic increases of WBC counts since the 15th day were also observed in both treated groups. In Spirulina- treated rats, the neutrophil count was enhanced precociously (since the 15(th) day). The overall lymphocyte population as well the T cell number has gradually augmented according to the treatment duration in all treated rats. Furthermore, Panax ginseng treatment has exhibited significant greater effects on lymphocyte and T cell counts than Spirulina platensis treatment. These results suggest that these 2 biomedicines positively interfere with bone marrow cellular production and with immune cellular response and may be useful as adjuvant treatment of anaemia or of immune deficiency.