Browsing by Author "Karaca, Turan"

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Depression of Glucose Levels and Partial Restoration of Pancreatic Β-Cell Damage by Melatonin in Streptozotocin-Induced Diabetic Rats
(Springer Heidelberg, 2006) Kanter, Mehmet; Uysal, Hamdi; Karaca, Turan; Sagmanligil, Hulya Ozdemir
Diabetes mellitus is a common but serious metabolic disorder associated with many functional and structural complications. Glucose metabolism is disturbed due to an absolute or relative insulin deficiency. The experiment was carried out to determine the effect of melatonin on blood glucose and insulin concentrations, and histopathology of pancreatic beta-cells in streptozotocin (STZ)-induced diabetic rats. The rats were randomly allocated into one of the four experimental groups: group A (control), group B (diabetic untreated), group C (diabetic treated with melatonin for 6 weeks) and group D (diabetic treated with melatonin for 8 weeks); each group contained ten animals. Diabetes was induced in B, C and D groups by a single intraperitoneal (i.p.) injection of STZ (50 mg/kg, freshly dissolved in 5 mmol/l citrate buffer, pH 4.5). The rats in melatonin-treated groups were subjected to the daily i.p injection of 10 mg kg(-1) of melatonin for 6 or 8 weeks starting the day after STZ injection. Control and diabetic untreated rats were injected with the same volume of isotonic NaCl as the melatonin treated groups. Almost all insulin-positive beta-cells were degranulated, degenerated or necrotic in the STZ-treated rats leading to decrease in insulin secretion and an increase in blood glucose concentration. Melatonin treatment caused a sharp decrease in the elevated serum glucose, a slight increase in the lowered serum insulin concentrations and small partial regeneration/proliferation of beta-cells of islets. It is concluded that the hypoglycemic action of melatonin could be partly due to small amelioration in the beta-cells of pancreatic islets causing a slight increase in insulin secretion, it is mostly due to the extrapancreatic actions of the melatonin.
Distribution of Eosinophil Granulocytes and Mast Cells in the Reproductive Tract of Female Goats in the Preimplantation Phase
(Springer, 2009) Karaca, Turan; Yoruk, Mecit; Uslu, Sema; Cetin, Yunus; Uslu, Baris Atalay
Changes in eosinophil granulocytes and mast cells post-insemination may affect conceptus implantation, but information regarding the numbers of such cells in the mammalian reproductive tract is limited. This study investigated the preimplantation distribution of eosinophil granulocytes and mast cells (MCs) in the reproductive tract organs of female goats. Uterus, uterine cervix and uterine tubes samples were obtained at slaughter. Cornu uteri were washed in phosphate buffer solution (each animal contained at least one embryo). Tissues were fixed in 10% neutral buffered formol, Carnoy solution and Mota's fixative (basic lead acetate) for 48 h and embedded in paraffin. Six-micrometre-thick sections were stained with Congo red (for eosinophil granulocytes) and toluidine blue in 1% aqueous solution at pH 1.0 for 5 min (for MCs). In the uterus, MCs occurred in highest numbers in the myometrium. Higher MC numbers were observed in uterus, uterine and uterine tubes in the preimplantation (experimental) group (cycle synchronised through 7 days intravaginal sponge with 0.3 g P-4) compared with the control group (P < 0.05). Eosinophil granulocyte numbers were significantly higher in the experimental group than in the control group (P < 0.05). These results indicate preimplantation-related changes in numbers of eosinophil granulocytes and MCs in goat reproductive tract organs.
The Effect of Glutathione Treatment on the Biochemical and Immunohistochemical Profile in Streptozotocin-Induced Diabetic Rats
(Springer, 2013) Yur, Fatmagul; Dede, Semiha; Karaca, Turan; Yegin, Sevim Ciftci; Deger, Yeter; Ozdemir, Hulya
This study investigated the possible role of glutathione (GSH) in diabetic complications and its biochemical safety in experimental diabetic rats. Serum biochemical parameters and the histology of the pancreas were investigated. Seven rats were separated as controls. To create the diabetes in rats, 45 mg/kg single-dose streptozotocin (STZ) was administered i.p. The treatment was continued for 1 month. STZ was administered to the diabetes + GSH group, then reduced GSH, dissolved in isotonic salt solution (200 mg/kg), was applied i.p. two times a week. The GSH group received i.p. GSH. Serum biochemical parameters were determined by autoanalyzer. Immunohistochemical procedures were used to determine the percentage of the insulin-immunoreactive beta-cell area in the islets of Langerhans. The biochemical parameters changed to different degrees or did not change. Pancreatic cells of the control and GSH groups were healthy, but in the diabetic and GSH-treated diabetic groups we found damage in different numbers. The results from these analyses show that GSH supplementation can exert beneficial effects on pancreatic cells in STZ-induced diabetic rats and can safely be used for therapy in and protection from diabetes and complications of diabetes.
Effect of Grape Seed Extract on Lipid Peroxidation, Antioxidant Activity and Peripheral Blood Lymphocytes in Rats Exposed To X-Radiation
(Wiley, 2007) Enginar, Hueseyin; Cemek, Mustafa; Karaca, Turan; Unak, Perihan
The present studies were designed to evaluate supplemental grape seed extract (GSE) and vitamin E supplements on lipid peroxidation, on antioxidant systems and peripheral blood lymphocytes in rats exposed to x-rays. Three groups of rats were investigated: a control group (CG) received intraperitoneal (i.p.)physiological serum I mL/day (n = 10), i.p.; a vitamin E group (VG) received 50 mg/kg/day (n = 10); an i.p. grape seed extract group received 50 mg/kg/day (n = 10). Four weeks later, a 6 Gy radiation dose was given to the rats. Blood samples were taken 24h later after irradiation and lymphocyte, malondialdehyde (MDA), reduced glutathione (GSH), nitrate, nitrite, reduced ascorbic acid, retinol, beta-carotene and ceruloplasmin concentrations were analysed. The levels of GSH (p < 0.05), retinol (p < 0.001), fi-carotene (p < 0.05) and ceruloplasmin concentration (p < 0.001) in the GSE group were found to be higher than in the control group but the level of MDA (p < 0.001) and nitrite concentration (p < 0.05) in rats supplemented with GSE were found to be lower than in the control group. The results indicate that GSE enhanced the antioxidant status and decreased the incidence of free radical-induced lipid peroxidation in blood samples of rats exposed to x-radiation. The antioxidant effect of GSE given to animals was more effective than vitamin E administered before whole-body irradiation in rats. Copyright (c) 2007 John Wiley & Sons, Ltd.
Effects of Extract of Green Tea and Ginseng on Pancreatic Beta Cells and Levels of Serum Glucose, Insulin, Cholesterol and Triglycerides in Rats With Experimentally Streptozotocin-Induced Diabetes: a Histochemical and Immunohistochemical Study
(Medwell online, 2010) Karaca, Turan; Yoruk, Mecit; Yoruk, Ibrahim H.; Uslu, Sema
This study investigated the effects of oral administration of extract of green tea (Camellia sinensis) and ginseng (American ginseng-Panax quinquefolium L.), given alone or together, on pancreatic beta-cells, blood glucose, insulin, cholesterol and triglyceride levels in rats with experimental diabetes induced by a single injection of Streptozotocin (STZ) (60 mg kg(-1), i.p.). Fifty adult Wistar Albino rats were used, 10 in each of these five treatment groups: Group A: healthy controls, Group B: STZ-induced diabetes (untreated), Group C: STZ-induced diabetes plus green tea extract (100 mg/kg/daily), Group D: STZ-induced diabetes plus ginseng root (400 mg/kg/daily) and Group E: STZ-induced diabetes plus ginseng root + green tea extracts as before. At the end of the 6 weeks experiment, blood samples were analysed for blood glucose, insulin, cholesterol and triglyceride levels and samples of pancreatic tissue were examined histochemically and immunohistochemically for endocrine islets and beta-cells. Overall, body weight decreased in groups B and C, serum insulin concentrations decreased slightly in groups C-E and total triglyceride levels of blood decreased significantly (p<0.05) in groups B and C compared with control, D and E groups. Histopathological examination showed that degenerative changes in pancreatic beta-cells in STZ-treated rats were minimised to near normal morphology by administration of ginseng (Group D) and ginseng+green tea (Group E) and there was increased intensity of immunohistochemical staining for insulin in these groups. Degeneration of islets of Langerhans beta-cells and weak insulin staining was observed for green tea alone (Group C). These findings demonstrate that ginseng or combined ginseng + green tea decreases blood glucose levels in diabetic rats and increases preservation of P-cells, perhaps by lowering oxidative stress.
Effects of Green Tea and Ginseng on Villus Length and Crypt Depth and on the Distribution of Mast and Goblet Cells in the Small Intestine of Rats With Streptozotocin (stz)-Induced Diabetes
(Univ Philippines Los Banos, College, 2011) Karaca, Turan; Uslu, Sema; Yoruk, Mecit
Diabetes is characterised by abnormally high plasma glucose concentration and causes functional and morphological changes in many organs. This study investigated the effects of green tea extract, ginseng root, and green tea extract plus ginseng root on the micromorphology of and distribution of mast cells and goblet cells in the intestine of rats with streptozotocin (STZ)-induced diabetes. Thirty healthy Wistar albino rats were used, six in each of five groups as follows: Group A (without diabetes): as control, Group B: STZ-induced diabetes without treatment, Group C: STZ-induced diabetes treated with green tea extract alone (100 mg/kg/daily), Group D: STZ-induced diabetes treated with ginseng root alone (400 mg/kg/daily) and Group E: STZ-induced diabetes treated with a combination of green tea extract and ginseng root (dosage as in group C and D). Blood samples were analysed for blood glucose at the end of the six week experiment period. A significant decrease (P<0.05) in serum glucose was observed in treated diabetic groups C, D and E compared with untreated diabetic group B. Mast cell number was significantly lower (P<0.05) in the lamina propria and submucosa of the small intestines, but the number of goblet cells in the duodenum and jejunum was higher in the diabetic groups than in other groups (P<0.05). Goblet cell density in the small intestine tended to be higher in the untreated diabetic group than in the other groups. Green tea extract and ginseng root had no influence on villus height in the duodenum and crypt depth in the ileum of diabetic rats but both had an effect which is characterized by a lower villus height in the jejenum. Green tea extract and ginseng root promoted micromorphology of the small intestine and also caused changes in the distribution of mast and goblet cells of the intestine of diabetic rats.
Effects of Nigella Sativa L. and Urtica Dioica L. on the Numbers of Mast Cells in the Thymus, Spleen and Mesenteric Lymph Nodes of Rats With Carbon Tetrachloride Toxicity
(Medwell online, 2007) Yoruk, Mecit; Karaca, Turan; Uslu, Sema
This study investigated the effects of Nigella Sativa L. (NS) and Urtica Dioica L. (UD) on the number of Mast Cells (MCs) in Carbon tetrachloride (CCl4)-treated rats. A total of 35 healthy male Sprague-Dawley rats were divided into 5 equal groups. All groups, expect control group, were injected subcutaneously with 0.8 mL CCl4 per kg body weight twice a week for 45 days and then CCl4 + UD-treated, CCl4 + NS-treated and CCl4 + UD + NS-treated rats received a daily intraperitoneal injection of 0.2 ml, kg(-1) NS or/and 2 mL kg(-1) UD oils for 45 days starting on day 46. Thymus, spleen and mesenteric lymph nodes were sampled, stained with 1% aqueous toluidine blue and examined microscopically. The thymus, spleen and lymph nodes of CCl4- exposed animals contained significantly increased numbers of MCs compared with control group, CCl4 + UD-treated, CCl4 + NS-treated and CCl4 + UD + NS-treated rats (p<0.05). However, when exposed rats were also treated with UD, NS or UD + NS, a decrease in MCs was observed. These results demonstrate that UD and NS decrease the number of MCs induced by CCl4 in the lymphoid tissues of rats.
Effects of Oral Administration of Estradiol Valerate on Gonadal Sex Differentiation in the Rainbow Trout, Oncorhynchus Mykiss
(Medwell online, 2008) Guzel, Senol; Yoruk, Mecit; Gullu, Kenan; Karaca, Turan
This study investigated the effects of administering Estradiol Valerate (E2V) on gonadal sex differentiation in rainbow trout (Oncorhynchus mykiss, W., 1792). Rainbow trout fry of average weight 0.32 +/- 0.02 g, 65 days post fertilization (dpf), which had just started to feed were supplied with a feed containing E2V at a rate of 20 mg kg(-1) for 8 weeks (65-121 dpf). Fish samples were taken at random at 156 days (dpf,), 170, 191, 226 and 261 days and changes in fish gonads were analyzed histologically. At 156 dpf of the experiment, it was observed that oocyte formation had started in the fish gonads, suggesting feminization by the administration of the estradiol valerate. Intersex fish were observed at 170 dpf, while oocytes had gathered heads in gonads and sex transformation of some individuals had started at 191 dpf. Oocytes occupied the large areas of gonads and spermatogonia disappeared at 226 dpf as at 261 dpf. At the end of the experiment, sex compositions were 97% female, 3% intersex in the E2V administration group and 51% female, 49% female in the control group. There were no apparent differences between control group fish and hormone-treated fish as regards oocyte size.
Effects of Spirulina on the Number of Ovary Mast Cells in Lead-Induced Toxicity in Rats
(John Wiley & Sons Ltd, 2007) Karaca, Turan; Simsek, Nejdet
The present study investigated the protective effect of Spirulina against the lead-induced increase in mast cells in the ovary during the oestrous cycle of rats. In the ovary cortex and medulla of lead-exposed animals, there was a significant increase in the number of mast cells; however, when also treated with Spirulina, a decrease was observed. The number of mast cells when Spirulina (300 mg/kg) was used alone was not significantly different from that of the control group. These results indicate that Spirulina decreases the number of mast cells induced by lead in the cortex and medulla of rat ovary. Copyright (c) 2006 John Wiley & Sons, Ltd.
Hindi Lenfoid Organlarında (Timus, ve Bursa Fabricius) Yaşa Bağlı Olarak Mast Hücrelarinin Dağılımı
(2006) Yoruk, Mecıt; Karaca, Turan; Uslu, Barış Atalay
Bu çalısma, hindilerde bazı lenfoid organlarda yasa baglı olarak mast hücrelerinin dagılımının belirlenmesi amacıyla yapıldı. Arastırmada, yörede aynı kosullarda yetistirilen 15, 30 ve 120 günlük yaslarında ve her yas grubunda 6 adet olmak üzere toplam 18 Bronz hindi kullanıldı. Hindiler dekapite edilerek timus, dalak ve bursa Fabricius’dan doku örnekleri alındı. Alınan örnekleri Mota’nın bazik kursun asetat (BLA) tespit solüsyonunda tespit edildi. Mast hücrelerin demonstrasyonu için toluidine blue ve Alcian blue/safranin O boyama metotları kullanıldı. Timusda tüm yas gruplarında medullada korteksten daha fazla mast hücresi oldugu belirlendi. Timus ve bursa Fabricius’da 15. günde en yüksek seviyede olan mast hücre sayısı, 30 ve 120. günlerde anlamlı olarak düsüs gösterdi (P<0.05). Dalakta ise 30. günde 15. güne göre artma olmakla beraber 120. günde tekrar azaldıgı tespit edildi. Alcian blue/Safranin boyamasında safranin pozitif mast hücresine çalısılan yas gruplarında ve organlarda rastlanamadı. Sonuç olarak, yasa baglı olarak hindi lenfoid organlarında mast hücre dagılımının degistigi gözlendi. Anahtar Sözcükler: Mast hücresi, lenfoid organ, hindi, yas
Immunohistochemical Distribution of Glucagon -, Insulin -, Somatostatin -, Gastrin-, and Serotonin-Containing Cells in the Pancreas of the Van Cat
(Tubitak Scientific & Technological Research Council Turkey, 2014) Karaca, Turan; Kara, Adem; Simsek, Nejdet; Uslu, Sema; Tekiner, Deniz; Yoruk, Mecit
The regional distribution, relative frequency, and appearance of glucagon (A-cell)-, insulin (B-cell)-, somatostatin (D-cell)-, gastrin (G-cell)-, and serotonin (EC-cell)-secreting cells in the endocrine and exocrine pancreas of Van cats were examined using the immunohistochemistry method. Glucagon immunopositive A-cells were principally found in the central region of the islets of Langerhans, while insulin immunopositive B-cells were located in the periphery of the pancreatic islets. Moreover, several A- and B-cells were observed as only single cells or clusters of 2 to 3 immunopositive cells in the exocrine parenchyma and the pancreatic duct epithelium. Somatostatin and gastrin immunopositive reactivities were negligible in the peripheral regions of the pancreatic islets of Langerhans and in the exocrine parenchyma. However, serotonin-immunopositive EC-cells were observed in neither the endocrine islets nor any other sites of the tissue. The existence, regional distribution, and relative frequency of A-, B-, D-, G- and EC-cells in the pancreas of Van cats have been analyzed in this study for the first time. The immunopositivity and distribution of endocrine cells in the Van cat pancreas were determined to be partially different from those of other carnivorous species such as dogs and other cats.
In Vivo Radioprotective Effects of Nigella Sativa L Oil and Reduced Glutathione Against Irradiation-Induced Oxidative Injury and Number of Peripheral Blood Lymphocytes in Rats
(Wiley-blackwell, 2006) Cemek, Mustafa; Enginar, Huseyin; Karaca, Turan; Unak, Perihan
Radiotherapy is one of the most common therapies for treating human cancers. Several studies have indicated that irradiation induces reactive oxygen species (ROS), which play an important role in radiation damage of the cell. It has been shown that Nigella sativa L. (NS) and reduced glutathione (GSH) have both an antiperoxidative effect on different tissues and a scavenger effect on ROS. The purpose of this study was to determine the antioxidant and radioprotective roles of INS and GSH against irradiation-induced oxidative injury in an experimental model. The INS group was administrated INS (1 mL/kg body weight), the GSH group was injected GSH (150 mg/kg body weight) and the control group was given physiologic saline solution (1 mL/kg body weight) for 30 consecutive days before exposure to a single dose of 6 Gy of radiation. Animals were sacrificed after irradiation. Malondialdehyde, nitrate, nitrite (oxidative stress markers) and ascorbic acid, retinol, beta-carotene, GSH and ceruloplasmin (nonenzymatic antioxidant markers) levels and peripheral blood lymphocytes were measured in all groups. There were statistically significant differences between the groups for all parameters (P < 0.05). Whole-body irradiation caused a significant increase in blood malondial-dehyde, nitrate and nitrite levels. The blood oxidative stress marker levels in irradiated rats that were pretreated with NS and GSH were significantly decreased; however, nonenzymatic antioxidant levels were significantly increased. Also, our results suggest that NS and GSH administration prior to irradiation prevent the number of alpha-naphthyl acetate esterase peripheral blood T lymphocytes from declining. These results clearly show that NS and GSH treatment significantly antagonize the effects of radiation. Therefore, NS and GSH may be a beneficial agent in protection against ionizing radiation-related tissue injury.
İstanbul'da Satışa Sunulan Hazır Kıymaların Histolojik, Mikrobiyolojik ve Serolojik Kalitesi
(2004) Sevinç, İrfan; Yıldız, Ahmet; Yoruk, Mecıt; Başkaya, Ruhtan; Çakmak, Ömer; Karaca, Turan
Bu çalışma, İstanbul’da tüketime sunulan hazır kıymaların histolojik, mikrobiyolojik ve serolojik kalitesinin saptanması amacıyla planlandı. Bu amaçla İstanbul’daki kasaplardan, marketlerden ve şarküterilerden toplam 27 hazır kıyma örneği toplandı. Histolojik kesitlerden hazırlanan preparatlarda örneklerinin 3’ünde (%11.1) kıkırdak dokuya ve 3’ünde (%11.1) sindirim kanalına ait organ; 12’sinde (%44.4) çok fazla, 7’sinde (%25.9) fazla, 5’inde (%18.5) normal, 3’ünde (%11.1) az miktarda iskelet kası dokusu; 11’inde (%40.7) çok fazla, 10’unda (%37) fazla, 5’inde (%18.5) normal ve 1’inde (%3.7) az miktarda yağ dokusu saptandı. Mikrobiyolojik analizler sonucunda toplam aerobik mezofilik genel canlı, koliform, Escherichia coli, Koagulaz pozitif Staphylococcus aureus, Bacillus cereus, maya ve küf sayısı sırasıyla 2.7x106, 4.1x104, 7.2x103, 3.2x103, 9.5x103, 1.4x105 ve 5.7x104 kob/g olarak tespit edildi. Numunelerin 20’sinde (%74) sülfit indirgeyen anaerob bakteri ve 3’ünde (%11.1) salmonella pozitif bulundu. İncelenen örneklerde at ve domuz etine rastlanmadı. Sonuçta, histolojik ve mikrobiyolojik olarak incelenen kıymaların istenen kalitede olmadığı, serolojik olarak ise uygun oldugu kanısına varıldı.
İstanbul'da Tüketime Sunulan Köftelerin Histolojik, Mikrobiyolojik ve Serolojik Kalitesi
(2004) Başkaya, Ruhtan; Yoruk, Mecıt; Karaca, Turan; Yıldız, Ahmet; Çakmak, Ömer
Bu çalışma, İstanbul’da tüketime sunulan hazır köftelerin histolojik, mikrobiyolojik ve serolojik kalitesinin saptanması amacıyla planlandı. Bu amaçla İstanbul Anadolu yakasındaki Fast-Food ve süpermarketlerden 75 hazır köfte örneği toplandı. Histolojik kesitlerden hazırlanan preparatlarda örneklerin 4 (%5.4)’ ünde az ve 2 (%2.7)’ sinde çok az miktarda yenmeyen dokuya rastlandı. Mikrobiyolojik analizler sonucunda toplam aerobik mezofilik genel canlı, koliform, Escherichia coli, Koagulaz pozitif Staphylococcus aureus, maya ve küf sayısı sırasıyla 5.6x105, 5.2x103, 4.3x101, 3.2x103, 2.6x104 ve 9.6x104 kob/g olarak tespit edildi. Numunelerin 9 (%12)’ unda sülfit indirgeyen anaerob bakteri ve 4 (%5.4)’ ünde salmonella pozitif bulundu. İncelenen örneklerde at ve domuz etine rastlanmadı. Sonuç olarak, İstanbul’un Anadolu yakasında tüketime sunulan köftelerin histolojik ve mikrobiyolojik olarak istenen kalitede olmadığı, serolojik olarak ise uygun oldugu saptandı.
Mast Hücre Heterojenitesi
(2005) Yoruk, Mecıt; Karaca, Turan
Mast hücreleri organizmanın histamin ve heparin depo hücreleridir. Bağdokunun bu iri hücreleri granülleri içerisinde histamin ve heparin ile birlikte hayvan türlerine göre değişik oran ve miktarda serotonin, eozinofilik kemotaktik faktör, N - asetil – B – glukosaminidaz, B – glukoronidaz, triptaz, kimaz, prostaglandin D2, RMCP I ve RMCP II gibi maddeleri de taşımaktadırlar. Bu hücreler, rutin histolojik kesitlerin hematoksilen–eozin boyamasında görülemezler. Bu derlemede, mast hücrelerinin granül içeriği, boyanma özellikleri ve tespitlere karşı verdiği reaksiyonları gibi farklı kriterlerle ortaya çıkan heterojenitesinin açıklanması amaçlanmıştır.
Morphological and Histometrical Studies on Distribution and Heterogeneity of Mast Cells in Chicken and Quail Digestive Tract
(2003) Karaca, Turan; Yörük, Mecit
ÖZET Karaca T, Tavuk ve Bıldırcın Sindirim Kanalında Mast Hücrelerinin Dağılımı ve Heterojenitesi Üzerine Morfolojik ve Histometrik Araştırmalar, Y.Y.Ü. Sağlık Bilimleri Enstitüsü Histoloji ve Embriyoloji Anabilim Dalı Doktora Tezi, Van, 2003. Bu çalışma, tavuk ve bıldırcın sindirim sistemi kanalındaki mast hücrelerinin dağılımı ve heterojenitesini belirlemek amacıyla yapıldı. Çalışmada, 10 adet Leghorn ırkı tavuk ve 10 adet Japon ırkı bıldırcın kullanıldı. Tavuk ve bıldırcınlar dekapite edilerek dil, kursak, özofagus, ön mide, mide, duodenum, jejunum, ileum, sekum ve kolondan doku örnekleri alındı. Alınan örnekler BLA (basic lead asetate - Mota), Carnoy ve IF AA (izotonic formaldehyde asetic acid) tespit solüsyonlarında tespit edilerek takip ve blokajı yapıldı. Hazırlanan histolojik kesitlere mast hücrelerinin dağılım ve heterojenitesini belirlemek amacıyla toluidine blue ve alcian blue - safranin boyamaları uygulandı. Mast hücrelerinin dağılımı ve heterojenitesinin sindirim kanalı organlarında farklılıklar gösterdiği ve bu organlar içerisinde her iki hayvan türünde de dil, kursak, özofagus ve ön midenin en fazla, müsküler midenin (taşlık) ise sayısal olarak en az olan organ olduğu belirlendi. Kullanılan tespit sıvılarından BLA ve Carnoy sıvılarının IFAA'ya göre mast hücrelerinin rnetakromatik özelliklerinin belirlenmesi ve sayısal dağılımın ortaya konmasında daha iyi sonuç verdiği kanısına varıldı. Alcian blue - safranin boyamasında her iki hayvan türünde de mukozal mast hücreleri alcian blue (+) reaksiyon verirken, safranin ile özofagus, ön mide, mide, kolon ve dilde pozitif reaksiyon gözlendi. Ayrıca bu organlarda seyrek olarak hem safranin (+) hem de alcian blue (+) hücreler de saptandı. Anahtar sözcükler: Bıldırcın, ışık mikroskobu, mast hücresi, sindirim kanalı, tavuk 62
A Morphological and Histometrical Study on Distribution and Heterogeneity of Mast Cells of Chicken's and Quail's Digestive Tract
(2004) Yoruk, Mecıt; Karaca, Turan
Bu çalışma, tavuk ve bıldırcın sindirim kanalındaki mast hücrelerinin dagılım ve heterojenitesini belirlemek amacıyla yapıldı. Çalısmada, 10’ar adet Leghorn ırkı tavuk ve Japon ırkı bıldırcın kullanıldı. Tavuk ve bıldırcınlar dekapite edilerek dil, kursak, özofagus, ön mide, mide, duodenum, jejunum, ileum, sekum ve kolondan doku örnekleri alındı. Alınan örnekler BLA (basic lead asetate – Mota), Carnoy ve IFAA (izotonic formaldehyde asetic acid) tespit solüsyonlarında tespit edilerek takip ve blokajı yapıldı. Hazırlanan histolojik kesitlere mast hücrelerinin dagılım ve heterojenitesini belirlemek amacıyla toluidine blue ve alcian blue - safranin boyamaları uygulandı. Mast hücrelerinin dagılımı ve heterojenitesinin sindirim kanalı organlarında farklılıklar gösterdigi ve bu organlar içerisinde her iki hayvan türünde de dil, kursak, özofagus ve ön midenin sayısal olarak en fazla, müsküler midenin ise en az olan organ oldugu belirlendi. Kullanılan tespit sıvılarından BLA ve Carnoy’un IFAA’ya göre mast hücrelerinin metakromatik özelliklerinin belirlenmesi ve sayısal dagılımın ortaya konmasında daha iyi sonuç verdigi kanısına varıldı. Alcian blue-safranin boyamasında, her iki hayvan türünde de, mukozal mast hücreleri alcian blue (+) reaksiyon verirken, safranin ile özofagus, ön mide, mide, kolon ve dilde pozitif reaksiyonlar gözlendi. Ayrıca bu organlarda seyrek olarak hem safranin (+) hem de alcian blue (+) hücreler de saptandı.
Preimplantasyon Sürecindeki Keçilerin Dişi Genital Kanal Organlarında Plazma Hücresi ve Alfa Naftil Asetat Esteraz Pozitif Lenfositlerin Dağılımı
(2009) Yoruk, Mecıt; Uslu, Barış Atalay; Karaca, Turan; Cetin, Yunus; Uslu, Barış Atalay
Bu çalışmada, keçilerde preimplantasyonun 6. gününde (Deneme grubu) ve anöstrusde (Kontrol grubu) dişi genital kanal organlarında plazma hücreleri ile alfa-naftil asetat esteraz (ANAE) (+) lenfositlerin dağılımının belirlenmesi amaçlandı. Çalışmada 2-4 yaşlarında, 12 adet renkli tiftik keçisi kullanıldı ve 2 eşit gruba ayrıldı. Keçilerden alınan doku örneklerinden bir kısmından kriyostat kesitler hazırlanarak ANAE (+) lenfositleri belirlemek için ANAE enzim demostrasyonu yapıldı. Doku örneklerinin diğer yarısı formol-alkol ile tespit edilerek plazma hücrelerinin belirlenmesi için rutin parafin kesitler hazırlandı. Kontrol grubuna göre preimplantasyon (deneme) grubunda hem ANAE (+) lenfositlerin hem de plazma hücrelerinin sayısının azaldığı belirlendi. Bununla birlikte, plazma hücrelerinin sayısının deneme grubunda korpus uteri, kornu uteri ve tuba uterinada, kontrol grubuna göre istatistiksel olarak anlamlı derecede azaldığı belirlendi (p<0.05). ANAE (+) lenfositlerin deneme grubunda en yoğun olarak kornu uteride, en az olarak da serviks uteri ve tuba uterinalarda tespit edildi. Sonuç olarak, preimplantasyon sürecinde keçi genital kanal organlarında, plazma hücreleri ile ANAE (+) lenfositlerin sayısal dağılımlarının fizyolojik değişimlere bağlı olarak değiştiği belirlendi.
Protective Potential of Royal Jelly Against Carbon Tetrachloride Induced-Toxicity and Changes in the Serum Sialic Acid Levels
(Pergamon-elsevier Science Ltd, 2010) Cemek, Mustafa; Aymelek, Fatih; Buyukokuroglu, Mehmet Emin; Karaca, Turan; Buyukben, Ahmet; Yilmaz, Fatma
Royal Jelly (RJ) is used in the Turkish folk medicine for the treatment of number of disorders. The present study describes the hepatoprotective and antioxidant activities of the RJ against carbon tetrachloride (CCl4)-induced acute liver damage. Sprague-Dawley rats were used for the experiment. CCl4 (0.8 ml/kg; s.c.) and RJ (50, 100, 200 mg/kg: orally) were given every other day, for 20 days. Malondialdehyde, reduced glutathione in whole blood and tissues: ceruloplasmin, sialic acid, ascorbic acid, retinol, beta-carotene and liver enzymes levels in serum were measured. Additionally, histopathological alterations in the liver were examined. RJ exerted the significant protective effect on liver damage as well as on oxidative stress induced by CCl4, resulting in reduced lipid peroxidation and improved endogenous antioxidant defence systems. It also reduced the elevated levels of liver enzymes. Histopathological study further confirmed the hepatoprotective effect of RJ, when compared with the CCl4 treated control groups. In conclusion, present study reveals biological evidence that supports the use of RJ in the treatment of chemical-induced hepatotoxicity. (C) 2010 Elsevier Ltd. All rights reserved.
Ruminant Plasentalarının Yapı ve Fonksiyonu
(2010) Karaca, Turan; Yoruk, Mecıt
Ruminantlar, epiteliokoryal ve kotiledonlu plasentaya sahiptirler. Ruminant plasentalarınınkarakteristik bir özelliği, trophoblastların farklı hücre popülasyonları taşımasıdır. Ruminantplasentaları, gebelikle ilişkili farklı glikoproteinler (PAGs), plasental laktojenler, büyüme faktörleri veKunitz domain proteinleri (TKDP) salgılar. Bu derlemede, ruminant plasentalarının yapısı, özelliklerive fonksiyonları hakkında değerlendirmeler sunulmuştur.