Browsing by Author "Karadaş, F."
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Article The Effect of Color Pigments Detected in Commercial Layer Diet on Egg Yolk Pigment Level(Centenary University, 2021) Alataş, E.; Kurbal, Ö.F.; Karadaş, F.In this study, egg samples and feed samples were taken from 32-week-old Novagen, 44-week-old Tinted, 45 and 58-week-old Lohman laying hens reared in commercial egg farms in Muş and Van provinces for three months with 1-month intervals. Total carotene, lutein, zeaxanthin canthaxanthin, apoester and beta-carotene as an individual carotene, vitamin A (retinol) and vitamin E content of egg yolk and feed samples were determined by HPLC. In this study, the roche scale and Minolta L*, a * and b * values of egg yolk were determined on the base of breed and periods. Egg yolk roche pigment scale results showed that except Tinted breed chickens in the second period, in general Lohman breed chickens were significantly higher in the 1st, 2nd, 3rd periods than Novagen and Tinted breeds egg yolk. Minolta a * values showed that generally egg yolks of Lohman chickens were darker red than Novagen egg yolks. While, minolta b* values were not statistically different among breeders in the second period, it has been observed that Novagen and Tinted breed hens egg yolks were significantly yellower than Lohman hen egg yolk in the first period, but the opposite was observed in the third period. While egg yolk total carotene contents were found to be lowest in Tinted breed eggs in the first period, no difference was found in the second period and the lowest total carotene content was found in Novagen breed chicken eggs in the third period. The results of this study concluded that the pigment and vitamin content of each egg in the market may vary as a reflection of consumed feeds. © 2021, Centenary University. All rights reserved.Article The Effects of Age in Layer on Accumulation of Oil-Soluble Antioxidants (Caroteonids, Vitamin E and Vitamin A) Concentration in the Egg Yolk(Centenary University, 2016) Karageçılı, M.R.; Karadaş, F.In this study laying hen diet’s total carotene, vitamin A and vitamin E levels and their effects with hens age how affected their accumulation in egg yolk were investigated. For this aim, egg yolk samples were obtained from Lohmann layers in 33rd, 43rd and 53rd weeks and Hy-Line layers in 48th, 58th and 68th weeks respectively. Egg yolk’s total carotenoid content was measured as 19.93, 16.85 and 18.14 (µg g-1) for Lohmann (p<0.05) and 16.72, 17.00, and 18.32 (µg g-1) for Hy-Line respectively (p>0.05). Furthermore egg yolk’s total vitamin A content was measured 3.86, 4.34 and 3.44 (µg g-1) for Lohmann and 7.64, 3.55 and 3.45 (µg g-1) for Hy-Line respectively (p<0.05). In addition to these egg yolk’s total vitamin E content was measured as 110.55, 111.03 and 115.86 (µg g-1) for Lohmann and 99.31, 97.89 and 110.01 (µg g-1) for Hy-Line respectively (p>0.05). As a result layers got old their egg size increase and nutrients in per unit volume decreased but this drawback could be compensated by reformulating diet formulations. © 2016, Centenary University. All rights reserved.Article Effects of the Melatonin and Vitamin E (alpha-Tocopherol Acetate) as Antioxidants on Biochemical Blood Parameters, Lipid Profile, and Muscle Vitamin E Concentration in Awassi Lambs Fed a High-Energy Diet and Normal Diet(Razi Vaccine and Serum Research Institute, 2023) Ismail Mahmud, K.; Karadaş, F.Health specialists currently suggest low-cholesterol diets, suggesting that cholesterol in the form of high-density lipoprotein (HDL) reduces the risk of chronic atherosclerosis. The large volume of literature describes the biological roles of vitamin E and its application to preventing disease and improving the health and productive performances of farm animals. The present study aimed to evaluate the effects of vitamin E (Alpha-tocopherol acetate) supplementation and melatonin implants on biochemical blood, lipid profile and muscle vitamin E content of Awassi male lambs fed by a high and normal diet in Iraq. The lambs were divided into teen groups as control normal energy diet T1 (NED) T2 (HED) concentrated lamb fattening feed. Two levels of melatonin (18 and 36 mg implant) were applied to T3, T4, T5, and T6 treatment and 2 levels of Vitamin E (Alpha-tocopherol acetate) diet 200 mg/kg, 400 mg/kg to T7. T8. T9 and T10, respectively. Results from the present study indicate that Vitamin E 200, 400 mg/lamb/day and melatonin implantation 18 mg, 36 mg/lamb/day significantly (P<0.05) increased total protein in serum while decreasing globulin level, glucose concentration in serum, melatonin implantation 36 mg/lamb and vitamin E 400 mg/lamb/day recorded significantly (P<0.05). The same effect on decreasing cholesterol concentration in serum 42.6mg\dl, 40.5 mg\dl, respectively, compared to nontreated groups. Vitamin E 200 mg/kg/lamb recorded the lowest AST level in serum, 43.3. Lambs implanted with Melatonin 36 mg/lamb and fed a high-energy diet (T8) resulted in a significant decrease of serum ALT activity (P<0.05) in comparison to other treated groups 12.7 U/L was achieved. Lambs fed a normal energy diet with vitamin E 200 mg/kg/lamb (T4) exceeded other treated groups, decreasing ALT serum levels by 9.35 U/L. Interestingly, muscle vitamin E concentrations for lambs received 200, 400 mg/lamb/day on the 2nd, 7th and 14th days of the storage period, and fed high energy diet (T10) or normal energy diet (T5) were significantly higher compared to control group (T1, T6). Copyright © 2023 by Razi Vaccine & Serum Research Institute.Article The Importance of Maternal And/Or in Ovo Antioxidant Feeding for Gene Expression and Performance in Poultry(Centenary University, 2017) Karageçılı, M.R.; Karadaş, F.At the present time, the nutrient requirements were increased with increasing of embryo metabolic rate according as the intensive selection applied to commercial poultry. Failure to meet this changing nutrient requirement causes adverse effects on parameters such as embriyonic development, hatching power, hatching quality and post-hatching performance. Poultry eggs contain large amounts of maternally-derived antioxidants that protect the embryo from oxidative damage caused by intense embryonic metabolism. There are few experimentally (in ovo and maternally feeding) studies have tested the effects of experimental change in yolk concentration of a major antioxidant on other antioxidant content of egg yolk, embryo and post hatch chick survive, whether, oxidative stress were affected from manipulation of antioxidants or not, to compare effects of this on postnatal chick development. At the same time, it has been reported that to increase breeder’s productivity and postnatal survival rate, breeders can transfer antioxidants from feed to egg as a part of complex epigenetic mechanism. Maternal and in ovo antioxidant feeding increase antioxidant level in the embryo tissues to be used by embryo in the critical period therefore it positively effects embryo viability, increases hatching weight and improves post-hatching feed consumption and yield. This positive effect was related to increase of the number of expressing genes with epigenetic effects, due to previous studies were determined that this effect was continuing for a few generations. In this review, articles on gene expression relationship with increased antioxidant concentration of embryo via maternal feeding or in ovo feeding have been reviwed. © 2017, Centenary University. All rights reserved.Article Investigation of Aflatoxin in the Completed Feed That Produced in the Van Basin(Centenary University, 2016) Rüstemoğlu, V.; Karadaş, F.In this study; it has been investigated that aflatoxins B1, B2, G1, G2 in the completed animal feed produce in the manufactures located in the Van basin. Feed samples were collected from 5 different commercial feed manufactures at the three different time and aflatoxins were analyzed by Shimadzu Prominence HPLC. The results showed that 67% rate of feed samples were contamined with AFB1 0.12-4.74 µg kg-1; 44% rate of feed 0.01-4.27 µg kg-1 AFB2; 4% rate of feed 0.11-0.52 µg kg-1 AFG1 and 40% 0.14-10.30 µg kg-1AFG2 respectively. It has been showed that obtained results of aflatoxin levels in the completed animal feed were lower than acceptable upper limit and were not at the risk level in the Van Basin. However it has been determined that there is still 67% of feed were contamineted with aflatoxins. © 2016, Centenary University. All rights reserved.Article Is It Possible That Pecan Nut (Carya Illinoinensis) Shell as an Organic Material May Be an Alternative Feed Additive(Centenary University, 2018) Kor, D.; Karadaş, F.; Karageçili, M.R.Consumption of natural foods has become important day after day and organic food production has also become popular as a result of increasing demand of consumer. Therefore; industry and science world, for organic animal food production focuses on the waste products generated in plant production and the availability of these natural products in animal nutrition has been increasingly discussed in recent years. The shell which is released during the production of pecan nut (Carya illinoinensis) is considered an organic waste material. Although there have been a lot of research on the pecan nut, in recent years the number of research articles associated with the nutritional composition and some phytochemical properties of pecan nut shell that related human and animal health is low and this shows that it is just beginning to be a subject of scientific work in this area. It has been underlined that pecan nut shell has high fiber content, antioxidant and antimicrobial properties according to reported data in the recent publications. In the view of such data, it may be thought that pecan nut shell can be used feed additive, like a prebiotic, supporting immune system and stopping the development of some pathogens. In addition; it is also interesting to note that there is the protective function of the pecan nut shell which suppresses the growth of some microorganisms that can cause health problems in humans due to the meat in cooked and preserved inappropriate conditions. Thus, it is concluded that pecan nut shell as a natural and a new product has potential subjected to research area of animal nutrition as feed additive. © 2018, Centenary University. All rights reserved.Article The Level of Fat-And Water-Soluble Antioxidants in Eggs of Free-Range Geese During a Production Season(Centenary University, 2023) Akyildiz, Z.; Karadaş, F.In this investigation, egg yolk and egg white water and fat-soluble antioxidant concentrations of geese eggs were determined during a production season according to months and weeks. Breeders consumed 100 g commercial layer feed per day during a production season. The farm was located at a semi-open prison of the Ministry of Justice in Van city. Fat-soluble (vitamin A, E, total and individual carotene) of egg yolk and water-soluble (ascorbic acid and GSH) antioxidants of egg white of geese were measured in February, March, April and May per week gazed in pasture conditions. Roche Yolk Color Fun (RYCF) values and Minolta (L* brightness, a* redness, and b* yellowness) values varied according to months and statistically significant differences were observed (p <0.05). RYCF, a * redness, and b *yellowness values were the lowest in February and L* brightness values were the highest in February (p <0.001). According to months (February-May), the egg yolk concentration of vitamin A (retinol) were 3.05±0.11, 1.84±0.10, 1.93±0.09, 2.84±0.19 µg g-1, total vitamin E were 26.87±1.80, 25.07±1.64, 38.16±1.71, 34.30±1.89 µg g-1, and total carotene were 15.49±1.44, 19.50±1.79, 42.39±1.99, 44.30±2.03 µg g-1 (p <0.05), respectively. In this study, lutein, cis-lutein, zeaxanthin, apoester, canthaxanthin, and beta-carotene were identified as individual carotene in goose egg yolks. Glutathione (GSH) and Vitamin C or ascorbic acid (AA) were detected in geese eggs white in the last two weeks of February and four weeks of March, April, and May. The results were recorded as: 0.91±0.14, 1.83±0.19, 2.69±0.15, 1.97±0.09 µg g-1 for AA and 7.71±1.86, 33.22±2.14, 45.37±2.41, 38.75±1.50 µg g-1 for GSH respectively. Both water-soluble GSH and AA data were significantly lower in February and were significantly higher in April (p <0.05) compared to other months. © 2023, Centenary University. All rights reserved.