Browsing by Author "Karadag, H."

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Biochemical and Histochemical Study on the Hypothalamus at Some Periods of Starvation and Refeeding After Starvation
(Asian Journal of Chemistry, 2007) Temur, A.; Atli, M.; Karadag, H.; Donmez, H. H.; Askerov, F.
This study was carried out to determine biochemical and histochemical alterations in the lateral (feeding centre) and ventro-medial (satiety centre) nuclei of hypothalamus in rats starved and refed after some periods of starvation. 180 Wistar albino rats were used in the investigation. The rats were starved for a periods of 1, 3, 5 and 7 d. The rats starved 5 d were refed for a periods of 5, 10, 15 and 30 d. The activity of the neutral peptide hydrolase was biochemically determined in the hypothalamus. In addition, histochemical investigation was made in the feeding and satiety centres. It was observed that the activity of the neutral peptide hydrolase became closer to the levels of the control group at 30th d of refeeding after 5 d starvation. It was concluded that the feeding centre was more sensitive to starvation than the satiety centre and the satiety centre was restored earlier than the feeding centre.
A Morphological and Stereological Study on Calculating Volume Values of Thoracic Segments of Geese
(Via Medica, 2019) Cakmak, G.; Karadag, H.
Background: In this study, the total volume of the thoracic segments of the spinal cord and volume densities of grey matter and white matter were examined by using stereological methods in adult geese having a weight of 3-4 kg. Materials and methods: Ten geese were used as material without sex discrimination. All animals were perfused with 10% formaldehyde. In addition, after perfusion, the geese were kept in 10% formaldehyde for 1 week. Afterwards, thoracic spine was removed and thoracic part of spinal cord was excised. 5 mu m thick sections were taken from these tissue samples by microtome. The cross-sectional series were obtained by sampling from each segment at 250th section. Twelve sections were taken from tissue specimens of every segment. The sections were stained by using haematoxylin-eosin and photographed on a microscope. Results: By using the Cavalieri's Principle, the volume densities (volume fractions) of thoracic segments of spinal cord, volume of white matter, and volume of grey matter in segments were calculated. Conclusions: In the study, total volume of the thoracic segment, volume of white matter, and the volume of grey matter, and the ratio of these volume values to each other were calculated. The SHTEREOM 1.0 software was used for calculating the volume of section specimens.
Morphology of the Venous System of the Heart in the Van Cat
(2003) Aksoy, G.; Karadag, H.; Ozudogru, Z.
Morphology of the veins draining the heart was studied in the Van cat. Veins draining the heart were the great cardiac vein, the middle cardiac vein, the right cardiac veins and the smallest cardiac veins. No anastomosis was observed between the terminal branches of the paraconal interventricular vein and the subsinuosal interventricular vein. The coronary sinus was formed by the great cardiac vein and the middle cardiac vein. The origins of the right cardiac veins had significant variations. The right semicircumflex vein was observed in three (50%) cats, and it terminated at the middle cardiac vein.
A Stereological Study on Calculation of Volume Values Regarding Lumbosacral Segments of Quails
(Blackwell Publishing Ltd, 2019) Cakmak, G.; Karadag, H.
In the study, total segment, grey matter and white matter volume values of lumbosacral segment of spinal cord in quails (Coturnix coturnix japonica) were stereologically examined. Regardless of sex, six adult quails were used as material. After the materials were perfused, they were dissected and their spinal cords were uncovered. Tissue samples were taken from each lumbosacral segment of spinal cord. After the tissue fixation process, 6–7 sections having a 5-µm thickness were separated in every 50th section as from the first section, where the tissue was seen, among tissue samples of each segment. These segments were stained with haematoxylin–eosin staining. They were photographed via a microscope. By using the Cavalieri's principle, the volume values of both the total segment, grey matter (GM) and white matter (WM) in each lumbosacral segment were separately calculated. All these calculations were performed by using SHTEREOM 1.5 program. As a result, total volume, grey matter and white matter volume values of lumbosacral segments and white matter/total volume, grey matter/total volume, and grey matter/white matter volume fractions were obtained. © 2019 Blackwell Verlag GmbH