Browsing by Author "Karagecili, Mehmet Resit"

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A Comparison of Fat-Soluble Antioxidants in Wild and Farm-Reared Chukar Partridges (Alectoris Chukar)
(Elsevier Science inc, 2017) Karadas, Filiz; Moller, Anders Pape; Karagecili, Mehmet Resit
This study assessed differences in antioxidant (carotenoid, retinol, retinol-ester, vitamin E and coenzyme Q(10)) composition of egg yolk and tissue in chukar partridges (Alectoris chukar) newly hatched from eggs of birds maintained in captivity on commercial maize-soybean based diets and birds from the wild whose diet was obtained from the natural environment. All eggs were incubated in a commercial hatchery. Day-old chicks from both groups were sacrificed and dissected for antioxidant analysis. Fat soluble antioxidant concentrations of egg yolk and tissues were determined by HPLC. Total carotenoids, retinol, alpha-tocopherol, and total vitamin E concentration of wild egg yolks were significantly higher compared to yolks from farm-reared birds (p < 0.05). However, gamma tocopherol, and coenzyme Q(10) were not significantly different in the yolks of either wild or farmed birds (p > 0.05). The concentration of total carotenoids in all tissues of wild chukar one-day old partridges was significantly higher than in farmed one-day old chukar partridge tissues (p < 0.05). Alpha tocopherol, free-retinol, retinal-esters and total vitamin A were significantly higher in most tissues of wild chukar when compared to farmed chicks (p < 0.05). Coenzyme Qio concentrations of heart, kidney and brain tissues of farm-reared chukar day old chicks were significantly higher than tissues from wild birds, although leg and breast tissues of wild chicks were significantly higher than in farmed birds (p < 0.05). These findings suggest that maternal access to antioxidants in the diet of farmed chukar partridges could positively influence fat soluble antioxidant concentrations in the egg yolk and tissues of day old chicks.
Effects of Egg Weight and in Ovo Injection of Α-Tocopherol on Chick Development, Hatching Performance, and Lipid-Soluble Antioxidant Concentrations in Quail Chick Tissues
(Copernicus Gesellschaft Mbh, 2018) Babacanoglu, Elif; Karagecili, Mehmet Resit; Karadas, Filiz
Lipid-soluble antioxidants can be more effective for chick development when provided via in ovo (IO) injection than when supplemented to the maternal diet. This study was conducted to evaluate the effects of egg weight (EW) and JO injection of alpha-tocopherol on chick development, hatching performance and lipid-soluble antioxidant concentrations in residual yolk sac (RYS), liver and brain tissues of quail chicks. Eggs were obtained from quail breeders at days 72 and 128 of age and incubated at 37.8 degrees C and 60 % relative humidity. Each egg was numbered and weighed prior to incubation, and the average EW of all eggs was 11.76 +/- 0.05 g. The eggs were divided into light (< 11.76 EW; EWL) and heavy (> 11.76 EW; EWH) groups (148 eggs per EW). Each EW group was divided into two IO groups: the control (non-injection) group and alpha-tocopherol group, in which 3.75 mg of alpha-tocopherol per egg was injected into the yolk sac followed by a 120 h incubation period. There were 64 eggs for each EW-IO treatment combination (16 eggs per EW per tray). The chick and RYS weights were significantly lower in the EWL group than in the EWH group. A significant EW by IO interaction suggested that IO increased the eggshell temperature of light quail eggs. The non-injected light eggs had a shorter hatching time due to the interaction of EW with IO. Hatchability, embryonic mortalities, and the cumulative hatching rate were not affected by EW or IO. The chick and middle toe lengths increased following IO administration (P < 0.05), which indicated that IO administration had positive effects on chick quality. However, shank length decreased following IO administration with an unchanged relative asymmetry (RA). IO significantly affected the absolute weights of the liver and heart and the relative weight of the heart, which was lower in the alpha-tocopherol group than in the control group. IO administration had no effect on total retinol and carotenoid concentrations in the RYS, liver and brain. Vitamin E, alpha-tocopherol and delta-tocopherol concentrations in the RYS, liver and brain were significantly higher in the alpha-tocopherol-treated chicks than in the control chicks. The highest brain alpha-tocopherol concentration was found in the alpha-tocopherol-treated chicks of the EWH group, indicating a significant interaction between EW and IO. The highest total lipid-soluble antioxidant concentrations were obtained following IO alpha-tocopherol treatment (P < 0.05), in the order brain < liver < RYS, but this pattern was not observed with delta-tocopherol. In conclusion, IO injection of alpha-tocopherol into the yolk sac affected the concentrations of tissue-specific lipid-soluble antioxidants in the RYS and tissues of newly hatched quail chicks, and EW had effects on various parameters.