Browsing by Author "Karakuş, F."

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Controlling Reproduction in Karakaş Ewes in Rural Conditions and Growth Characteristics of Their Lambs
(2005) Gökdal, Ö.; Ülker, H.; Karakuş, F.; Aşkin, Y.
The possibilities of synchronizing lambing and increasing reproduction by using exogenous hormones in Karakaş ewes raised under rural farm conditions and growth characteristics of their lambs were investigated. 112 Karakaş ewes at 2-5 ages were randomly assigned into treatment (n = 45) and control (n = 67) groups during breeding season. The ewes in treatment group were placed with 60 mg Medroxyprogesterone acetate (MPA) containing intravaginal passaries for 14 days and received 600 IU PMSG injections at passary withdrawal. Ewes in the control group were not treated and allowed to mate at their natural estrus. Ram introduction for both groups was performed in pasture in village common flock in free mating system. Litter size and twinning rates for treatment and control groups were 1.06 and 1.04; and 6.45 and 4.08 %, respectively, for the first two cycles related to passary withdrawal (P > 0.05). When the expected lambing time was considered, the proportions of the lambed ewes in the first 7 and 10 days were 70.97% and 70.97% for treatment, and 20.41% and 26.53% for control group. The differences in lambing rates in both periods between groups were significant (P < 0.05). Birth weight, 1st, 2nd, 3rd and 4th month live weights, weaning weight and daily live weight gain in treatment group lambs were 3.63 ± 0.13, 9.30 ± 0.41, 16.54 ± 0.50, 23.62 ± 0.77, 31.97 ± 0.66, 31.57 ± 0.92 and 0.221 ± 0.008 kg; and, in control group lambs were 3.26 ± 0.12, 10.11 ± 0.44, 17.80 ± 0.53, 26.30 ± 0.82, 34.59 ± 0.75, 34.42 ± 0.96 and 0.244 ± 0.008 kg, respectively. Except birth weight, treatment group lambs' live weights in all periods were lower than control ones (P < 0.05 and P < 0.01). The results of this study indicate that in order to run studies to control reproduction by using exogenous hormones in rural conditions successfully some precautions and technical support are required. © TÜBİTAK.
The Effect of Body Condition Score on Fertility, Live Weight and Some Body Measurements in Goats
(Centenary University, 2016) Karakuş, F.
The aim of this study was to determine the effect of body condition score at mating and kidding period on fertility traits, live weight and some body measurements in Saanen and Hair goats. The study was carried out on 49 head Saanen and 48 head Hair goats at the age of 2-5 years. Mating and kidding condition scores were determined as 2.24 and 1.84 in Saanen goats and 2.17 and 1.87 in Hair goats, respectively. The effect of condition score at mating on twinning rate (P<0.01) and survivability of kids (P<0.05) was significant; however, its effect on pregnancy rate, fecundity, litter size and infertility rate was insignificant. Besides the effect of condition score at mating on live weight in the same period was insignificant while it had significant effect on live weight after parturition (P<0.01). Condition score at kidding had a significant effect on live weight for both periods (P<0.05). The effect of body condition score on body measurements was not significant. According to the study results, it can be said that in Saanen and Hair goats, body condition score at the start of the mating season should be at least 2.5 and also this condition should be maintained during the period of gestation. © 2016, Centenary University. All rights reserved.
Effect of N-Hexane Extract From Tanacetum Argenteum (Lam.) Willd. Subsp. Argenteum on the Secretion of Proinflammatory Cytokines in Thp-1 Cell Line
(Society of Pharmaceutical Sciences of Ankara (FABAD), 2024) Arzuk, E.; Karakuş, F.; Albayrak, G.; Ergüç, A.; Tan, I.; Atiş, E.
Inflammation is an initial biological process that involves the activation of the immune system in response to injury, infection or exposure to toxic agents. During this process, cytokines, small proteins produced by immune cells, play a vital role in regulating the immune response. Inflammatory cytokines, including interleukins, tumor necrosis factor-α, nitric oxide, and interferon-gamma, initiate the immune response and promote inflammation. Natural products are frequently a source of potential anti-inflammatory compounds, and screening natural products can lead to the discovery of novel bioactive compounds. The present study aimed to investigate the effects of n-hexane extract from Tanacetum argenteum subsp. argenteum on the lipopolysaccharide-induced inflammatory response in human macrophages THP-1 cell. Cells were incubated with different concentrations of n-hexane extract, and the inhibitor effects of the extract exposure on various cytokine secretions were determined. The findings demonstrated that n-hexane extract dramatically decreased the levels of interleukin-6, interleukin-1β, and tumor necrosis factor-α in differentiated THP-1 cells, indicating the remarkable anti-inflammatory potential of the extract. The n-hexane extract inhibited the secretion of interleukin-6 and interleukin-1β even at the lowest dose of 1 μg/ml. However, a significant reduction in tumor necrosis factor-α secretion was observed at 5 μg/ml and above concentrations. Importantly, the results of the study indicated that both the n-hexane extract and its active component, parthenolide, exhibit comparable effects. Furthermore, in silico analysis of toxicogenomic data revealed the interactions between the active component of the n-hexane extract and interleukin-6, interleukin- 1β, and tumor necrosis factor. © 2024 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.
The Growth Traits of Karakaş and Its Crosses Lambs (F1) With Ile De France X Akkaraman (G1) Under Unlimited Suckling Regime
(2006) Gökdal, Ö.; Ülker, H.; Karakuş, F.; Cengiz, F.
In this study, the growth traits of Karakaş and (Ile de France × Akkaraman (G1)) × Karakaş crosses (F1) (IDFAK) lambs were investigated. Ewes in the flock were not milked and lambs were subjected to unlimited suckling regime without weaning. Least squares means of weights of IDFAK and Karakaş lambs at birth and 1-6th months were 4.67 and 4.08 kg (p<0.01), 9.16 and 9.49 kg, 14.87 and 15.44 kg, 22.31 and 24.12 kg, 30.84 and 32.51 kg, 35.34 and 33.79 kg, 39.51 and 35.99 kg, respectively. Least squares means of daily live weight gains of IDFAK and Karakaş lambs from birth to 1., 2., 3., 4., 5. and 6th months of age and between 3-6th months were 0.159 and 0.170 kg, 0.174 and 0.184 kg, 0.198 and 0.219 kg, 0.221 and 0.235 kg, 0.207 and 0.197 kg, 0.196 and 0.177 kg, 0.202 and 0.172 kg, respectively. The survival rates of IDFAK and Karakaş lambs until 60th day were 95.94% and 89.79%, respectively (p<0.05). It seems that (Ile de France × Akkaraman (G1)) × Karakaş crosses (F1) lambs reached to marketing age more short time than Karakaş lambs. It could be concluded that Ile de France x Akkaraman (G1) genotype is useful in improving lamb production in Karakaş sheep and desired growth could be obtained especially for cross lambs during 5th and 6th month age. © 2006 Asian Network for Scientific Information.
In Silico and In Vitro Anticancer Effects of Caffeic Acid Phenethyl Ester on Pancreatic Adenocarcinoma Cells
(Society of Pharmaceutical Sciences of Ankara (FABAD), 2023) Tanriverdı, Z.; Kuzu, B.; Eyol, E.; Karakuş, F.
Pancreatic adenocarcinoma is an aggressive and fatal malignancy due to the lack of early diagnosis and poor therapeutic response. At this point, determining the anticancer potential of non-toxic natural compounds is essential. Caffeic acid phenethyl ester is a bioactive compound with different activities. In this study, the toxicity of caffeic acid phenethyl ester was estimated by in silico methods in 14 pancreatic cancer cells, and its anticancer activity was evaluated in rat adenocarcinoma cells. According to the in silico results, caffeic acid phenethyl ester had anticancer properties without causing severe toxicity. Subsequently, we investigated the effects of caffeic acid phenethyl ester on rat pancreatic cancer (ASML) cells. Caffeic acid phenethyl ester reduced ASML cell viability by up to 27% in a dose-(5, 10, 20, 40, and 80 μM) and time-dependent (24, 48, and 72 h) manner. In the scratch assay, only 80 μM caffeic acid phenethyl ester statistically considerably inhibited ASML cell migration at 24 h. On the other hand, at 48 hours, all doses of caffeic acid phenethyl ester statistically remarkably decreased cell migration. Caffeic acid phenethyl ester also decreased ASML colony numbers at 5 μM and 10 μM compared to the control and completely suppressed colony formation at ≥ 20 μM. Our results revealed that caffeic acid phenethyl ester showed anticancer potential against human and mouse pancreatic cancer cells in silico and significantly inhibited the viability, migration, and colony formation of ASML cells in vitro. © 2023 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.
In Silico Drug Repurposing as Inhibitors Against Gsk-3β
(Hacettepe University, Faculty of Pharmacy, 2024) Deniz, E.; Karakuş, F.; Kuzu, B.
Tau, a protein associated with microtubules, is widely distributed throughout the central nervous system and promotes the polymerization, assembly, and stability of microtubules. Hyperphosphorylation of tau proteins leads to intracellular neurofibrillary tangles, which are the pathological hallmark of numerous neurodegenerative diseases and are collectively referred to as “tauopathies”. The most notable kinase identified in tau phosphorylation is glycogen synthase kinase 3 (GSK-3). Among the GSK-3 isoforms, GSK-3β has been linked to the pathophysiology of neurodegenerative diseases. Pharmacological inhibition of GSK-3β has been suggested as a potential therapeutic target for these diseases. In this study, the literature and databases were searched for potential inhibitory drugs against GSK-3β and 58 drugs were found. The drugs were filtered according to physicochemical-pharmacological properties and toxicity profiles via SwissADME, pkCSM, and ProTox-II, free web tools. After prefiltration, molecular docking was performed against GSK-3β with the remaining seven drugs (Nabumeton, Loxoprofen, Ketoprofen, Oxytetracycline, Benzoyl Peroxide, Naproxen, and Epinephrine Hydrochloride). According to the results, nabumetone had the best binding energy (-7.39 kcal/mol) and inhibition ability at the lowest concentration (3.8 µM) against GSK-3β among the seven drugs [compared to PF-04802367, a highly selective brain-penetrant kinase inhibitor]. Our results suggest that nabumetone may be a potential inhibitor of GSK-3β. © 2024, Hacettepe University, Faculty of Pharmacy. All rights reserved.
A Network Toxicology Analysis of the Molecular Pathways and Novel Targets in Tcdd-Induced Cardiovascular Toxicity
(Society of Pharmaceutical Sciences of Ankara (FABAD), 2024) Karakuş, F.
2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD), an environmental contaminant, disrupts multiple systems including endocrine, immun, nervous, reproductive, developmental, and cardiovascular. This study aimed to identify the molecular pathways and potential therapeutic targets for TCDD-induced cardiovascular toxicity using CTD, ShinyGO, STRING, GeneMANIA, ChEA3, MIENTURNET, and Cytoscape computational tools. The analysis identified the AGERAGE signaling pathway, blood circulation, and cytokine receptor binding as the top 3 among ten key molecular pathways, biological processes, and molecular functions associated with TCDD-induced cardiovascular toxicity. Additionally, ten hub proteins/genes were found to play a critical role, with NFKB1 being the most essential regulating transcription factor and hsa-miR-19a-3p and hsa-miR-125b-5p as the most crucial microRNAs. This study sheds light on the molecular mechanisms underlying TCDD-induced cardiovascular toxicity, revealing novel potential targets for therapeutic intervention. © 2024 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.
Network Toxicology for the Cardiovascular Toxicity Analysis of Tyrosine Kinase Inhibitors
(University of Ankara, 2024) Karakuş, F.
Objective: This study aims to explore potential molecular mechanisms and targets of cardiovascular toxicities caused by tyrosine kinase inhibitors. Therefore, toxicogenomic data mining was conducted focusing on sunitinib, sorafenib, pazopanib, axitinib, and their associations with cardiovascular diseases. Material and Method: Common genes between tyrosine kinase inhibitors and cardiovascular diseases were uncovered via comparative toxicogenomic databases. Additionally, protein-protein and gene-gene interactions were identified using STRING and GeneMANIA, respectively. Subsequently, hub proteins associated with tyrosine kinase inhibitor-induced cardiovascular diseases were determined through Metascape. Transcription factors and microRNAs related to this toxicity were identified using ChEA3 and MIENTURNET, respectively. Finally, gene ontology enrichment analysis and the most associated molecular pathways were identified using the DAVID database and Metascape, respectively. Result and Discussion: Toxicogenomic data mining revealed six genes common between tyrosine kinase inhibitors and cardiovascular diseases, with five of these genes (FLT1, FLT4, KDR, MAPK1, and MAPK3) identified as hub genes. Physical interaction was dominant among these hub genes (77.64%). Sunitinib, sorafenib, pazopanib, and axitinib generally downregulated the activities of these proteins. SOX17 and SOX18 were prominent among transcription factors, while hsa-miR-199a-3p was the most important microRNA associated with this toxicity. Moreover, the Ras signaling pathway was mostly associated with tyrosine kinase inhibitor-induced cardiovascular toxicities. These findings make a substantial contribution to understanding the processes underlying cardiovascular diseases induced by sunitinib, sorafenib, pazopanib, and axitinib. They also reveal novel potential therapeutic targets, including genes, proteins, transcription factors, microRNAs, and pathways. © 2024 University of Ankara. All rights reserved.
Possible Cardioprotective Mechanism of Action of Dexrazoxane, and Probable Human Topoisomerase Iiβ Inhibitors: an in Silico Analysis
(University of Ankara, 2022) Karakuş, F.; Kuzu, B.
Objective: The aim of this study was to determine which metabolite plays a role in the cardioprotective effect of dexrazoxane, and also to identify alternative compounds to dexrazoxane since clinical use of dexrazoxane is limited. For this purpose, the interactions of dexrazoxane and its three metabolites (B, C, and ADR-925), as well as the compounds, which reported to be inhibitors for topoisomerase VI (prototype of human DNA topoisomerase II beta), with human DNA topoisomerase II beta were investigated by molecular docking. Afterwards, the theoretical ADMET properties of all these compounds were determined Material and Method: The molecular structures were optimized by Gaussview 05 and Gaussian 03 package programs. AutoDock 4.2 software was used for molecular docking studies and the docking complexes were analyzed in 2D and 3D using the Discovery Studio Client 4.1 program. The pkCSM online program was used to calculate the theoretical ADMET parameters. Result and Discussion: As a result of molecular docking studies, it was determined that the B metabolite of dexrazoxane has a higher binding potential to human DNA topoisomerase II beta compared to both dexrazoxane and its other metabolites. The binding potentials of other compounds reported in the literature to human DNA topoisomerase II beta were radicicol>quinacrine>purpurin>9-Aminoacridine>hexylresorcinol, respectively. The results showed that the B metabolite of dexrazoxane plays an important role in the cardioprotective mechanism of action of dexrazoxane against anthracycline cardiotoxicity. In addition, it has been determined that other compounds, except purpurin, have the potential to cause toxicity. © 2022 University of Ankara. All rights reserved.