Browsing by Author "Karan, B.M."

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The Association Between Platelet-Derived Growth Factor Receptor-Β Polymorphisms and Idiopathic Pulmonary Fibrosis
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Kaya, Z.; Duran, S.; Gunbatar, H.; Sahin, E.S.; Karan, B.M.
Idiopathic pulmonary fibrosis (IPF) is a chronic interstitial lung disease with a dismal prognosis. Platelet-derived growth factor (PDGF) receptor-β (PDGFR-β) are a receptor tyrosine kinase that PDGFs activate. It has been reported that inhibiting PDGFR-β in IPF patients can slow and improve disease progression. However, the effects of PDGFR-β on IPF remain unknown, and no studies on PDGFR-β polymorphisms for IPF have been conducted. The purpose of this study is to look into the relationship between PDGFR-β gene polymorphisms (rs246395, rs2302273, rs3828610, rs138008832) and IPF disease. The study included eleven patients with IPF and twelve healthy controls. DNA was isolated from blood samples taken from all participants, and genotyping was performed using a StepOne plus real time PCR device. There was no statistically significant difference between the variables (age, gender, smoking, alcohol, and gastroesophageal reflux [GER]). There was no statistically significant difference between the patient and control groups in the allele and genotype frequencies of these polymorphisms. Furthermore, no statistically significant difference was found between patients' smoking, forced vital capacity (FVC) (normal, low), and GER data, as well as PDGFR-β variants (rs246395, rs2302273, rs3828610 and rs138008832). In order to determine the relationship between PDGFR-β gene polymorphisms and the risk of IPF, larger studies with more participants are required. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Evaluation of Tp53 Codon 72 Polymorphism in Esophageal Cancer Susceptibility in Eastern Anatolia Region of Turkey
(Yuzuncu Yil Universitesi Tip Fakultesi, 2021) Kaya, Z.; Almali, N.; Karan, B.M.; Gorgisen, G.
The tumor suppressor TP53 gene plays a key role in the regulation of cell cycle. Polymorphisms in this gene have been associated with many cancers including esophageal cancer (EC). Many studies in other populations have demonstrated that codon 72 polymorphism of TP53 gene contribute to the prediction of EC risk, especially in Asians. The aim of this study was to explore the effect of codon 72 polymorphism on the EC risk in eastern Turkey. The codon 72 polymorphism was genotyped by real time polymerase chain reaction (qPCR) with TaqMan SNP geno typing assay in 79 patients and 80 healthy control subjects. No statistically significant difference was observed in distribution of genotype and allele frequencies. Heterozygous Arg/Pro (CG) was the most frequent genotype in both patients and controls. Homozygous Arg/Arg (GG) genotype frequency was higher in patients than controls, but not statistically significant (p>0.05). However, tumor location in the lower part of the esophagus was significantly higher in non-C carriers (GG, Arg/Arg) compared to C-carriers (CG/CC) (p=0.01). G-carriers were also more likely to have poorer survival compared to patients with CC genotype (p=0.04). Our results suggest that the Codon 72 polymorphism was not associated with the EC in eastern Turkey. However, GG genotype (Arg/Arg) may have a role in tumor development at the lower location of the esophagus. Additionally, G carriers may exist the poorer survival compared to the non-G carriers (CC). Therefore, it is thought that individuals with CC genotype (Pro/Pro) may have better survival. © 2021, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.