Browsing by Author "Karaozan, Abdulselam"

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    Article
    Concurrent Detection of Five Yellow Dwarf Viruses ( B/Cydvs) in Wheat in Mardin (Turkey) and Phylogenetic Relationship of Bydv-Pav
    (Univ Agr Sci & veterinary Med Cluj-napoca, 2020) Karaozan, Abdulselam; Usta, Mustafa
    Wheat (Triticum aestivum L.) is a natural host of many viruses. Yellow dwarf viruses belonging to the Luteoviridae family are important virus species that cause economic loss by restricting wheat production worldwide. Surveys were conducted in 2017 to determine Yellow dwarf viruses (BYDV-PAV, BYDV-MAV, BYDV-SGV, BYDV-RMV, and CYDV-RPV) and their infection rates in wheat production areas in Mardin province. 400 fresh leaf samples collected were tested by Multiplex reverse transcription-polymerase chain reaction (m-RT-PCR). The overall infection rate was found to be 3%. BYDV-PAV has been identified as the most widespread virus with a 2.5% presence rate. It was found out that BYDV-SGV, CYDV-RPV, and BYDVRMV infections were lower, with rates of 1.75%, 0.5% and 0.25% respectively. In the current study, double infections were detected in 8 samples. The overall infection rate of the detected viruses (BYDV-PAV, BYDVSGV, CYDV-RPV, BYDV-RMV) was found to be lower than the records reported in previous similar studies. No BYDV-MAV infection was found in any of the wheat samples tested. The cDNA of the coat protein (CP) gene of a BYDV-PAV isolate randomly selected from virus-positive samples was cloned, bidirectionally sequenced, and the phylogenetic relationship revealed. According to the phylogenetic analysis with 19 different isolates in the NCBI database of BYDV-PAV Mardin isolate, it showed the highest genetic similarity by 95.52% with the Germany isolate (KY634926) while the lowest similarity rate was 89.22% with the Germany and Pakistan isolates (KY634886 and JQ811489). The presence of BYDV-PAV, BYDV-SGV, CYDV-RPV, and BYDV-RMV were reported for the first time with this survey study conducted in Mardin.
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    Master Thesis
    Molecular Characterizations of Some Virus Isolates and Investigation of Barley Yellow Dwarf Viruses (bydvs) in the Wheat Production Areas in Mardin Province by Multiplex Rt-Pcr
    (2019) Karaozan, Abdulselam; Usta, Mustafa
    Mardin ili ve ilçelerinde buğday üretim alanlarında Arpa sarı cücelik virüslerini (BYDV-PAV, BYDV-MAV, BYDV-SGV, BYDV-RMV ve CYDV-RPV) belirlemek amacı ile 2017 yılında survey çalışmaları yapılmıştır. Survey çalışması kapsamında toplanan 400 örnekte virüs tespiti için multipleks RT-PCR ve RT-PCR yöntemleri kullanılmıştır. Testlenen 400 örneğin 10'nunda BYDV-PAV (% 2.5), 7'sinde BYDV-SGV (% 1.75), 2'sinde CYDV-RPV (% 0.5) ve 1'inde BYDV-RMV (% 0.25) tespit edilmiştir. Pozitif izolatların 7'sinde BYDV-PAV+BYDV-SGV (%1.75) ve 1'inde BYDV-PAV+CYDV-RPV (% 0.25) karışık infeksiyon belirlenmiştir. Mardin ilinde buğday virüslerinin genel infeksiyon oranı % 3.0 olarak bulunmuştur. Testlenen 400 buğday yaprağı örneğinin hiçbirinde BYDV-MAV tespit edilememiştir. Yürütülen bu çalışmada Mardin ilinde BYDV-PAV, BYDV-SGV, BYDV-RMV ve CYDV-RPV virüsleri ilk defa tespit edilmiştir. Tespit edilen virüslerin kılıf protein gen bölgesinin karakterizasyonu için herbir virüs türünden bir pozitif izolat seçilerek kılıf protein gen bölgeleri karakterize edilmiştir. BYDV-PAV (Gen bankası Ulaşım No. MK732034) Mardin izolatının ve CYDV-RPV (Gen bankası Ulaşım No. MK732035) Mardin izolatlarının kılıf protein gen bölgelerinin tamımı, BYDV-RMV (Gen bankası Ulaşım No. MK955886) ve CYDV-RPV (Gen bankası Ulaşım No. MK940529) Mardin izolatların ise kılıf protein gen bölgeleri kısmi olarak klonlanıp DNA dizilemeleri gerçekleştirilmiştir.