Browsing by Author "Karapinar, Z."

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Detection and Sequence Analysis of Equine Gammaherpesviruses From Horses With Respiratory Tract Disease in Turkey
(Wiley, 2010) Ataseven, V. S.; Bilge-Dagalp, S.; Oguzoglu, T. C.; Karapinar, Z.; Guzel, M.; Tan, M. T.
P>The equid herpesvirus 2 (EHV-2) and 5 (EHV-5), identified agents of respiratory infections and keratoconjunctivitis cases in some equids, comprise a high degree of antigenic heterogeneity. Prevalence and genetic characterization of EHV-2 and EHV-5 strains from Turkey were investigated in this study. A total of 73 nasal swabs and 54 blood specimens were sampled from horses with respiratory tract diseases characterized by mucopurulent nasal discharge and occasional coughing. Overall, EHV-2- and EHV-5-specific DNA amplicons were obtained from 19.2% (14/73) and 21.9% (16/73) of horses tested by multiplex nested PCR. Sequences of EHV-2 and EHV-5 glycoprotein B (gB) gene were used in a phylogenetic analysis that included six EHV-2 and three EHV-5 isolates, which showed that the Turkish EHV-2 and EHV-5 strains have marked sequence divergence from European strains and from each other. Turkish EHV-2 isolates were divided into two distinct subdivisions, and a few isolates were located on a separate branch. This study provides the first epidemiological and phylogenetical report about EHV-2 and EHV-5 infections in Turkey.
Partial Sequence of the Orf1b Gene Fragment of Equine Arteritis Viruses Detected in Turkey and Phylogenic Analysis
(Ecole Nationale veterinaire Toulouse, 2013) Ataseven, V. S.; Oguzoglu, T. C.; Karapinar, Z.; Bilge-Dagalp, S.
The aim of this study was to document the first genetic information based on partial sequences of the ORF1b gene of nine Turkish (TR) equine arteritis virus (EAV) strains obtained from horses showing signs of respiratory tract disease. Partial sequencing of the PCR products based on ORF1b gene using EAV specific primer pairs followed amplification and purification was performed and was compared to European and American viruses recoded in GenBank to phylogenic analysis. The TR virus strains were found closely related to the strains that originated in North America and exhibited marked sequence heterogeneity from European strains and additionally the TR strains were relatively homogeneous and were placed in a separate cluster. This study documents for the first time the genetic of EAV genotypes in Turkey and will provide valuable insight for future studies on the molecular epidemiology of EAV.
Pathology and Phylogenetic Analysis of Capripoxvirus in Naturally Infected Sheep Sheeppox Virus
(Univ Agriculture, Fac veterinary Science, 2017) Karapinar, Z.; Ilhan, F.; Dincer, E.; Yildirim, S.
Sheeppox and goatpox is a contagious viral disease of sheep and goats characterized by fever, generalized papules or nodules in skin and mucosal surface. DNA virus belonging to the genus capripoxvirus. The disease with high mortality and morbidity causes significant economic losses in small ruminants. In this study, 20 tissues from 8 sheep which were considered having natural sheeppox based on the macroscopic and histopathologic evaluation were further investigated by immunohistochemical (iNOS, SP-A, HSP-70), and Polymerase Chain Reaction (PCR) method. Papules, typically pox lesions, noticeable on the skin surfaces were examined macroscopically. Immunohistochemically; iNOS and SP-A were most intensely stained whereas HSP-70 was low stained. PCR method was used for the detection of A29L gene of capripoxvirus. Positive samples obtained from sheep were used for molecular characterization. A phylogenetic analysis was performed using sequence of the partial A29L gene and by comparing with reference sheeppox viruses isolates obtained from Gene Bank. The results of the sequence analysis were similar among themselves, they were found different (99-100% identity) from the other sheeppox viruses around the world. This study provides firstly phylogenetic analysis of sheeppoxviruses from Van province in Turkey. (C) 2016 PVJ. All rights reserved
Seroprevalence of Visna-Maedi Virus (Vmv) and Border Disease Virus (Bdv) in Van Province and Around
(Arquivo Brasileiro Medicina veterinaria Zootecnia, 2018) Ameen, P. S. Mohammad; Karapinar, Z.
The present study investigated the seroprevalance of Visna Maedi Virus (VMV) and Border Disease Virus (BDV) infections in sheeps in regions in and around Van province, Turkey. Sample materials were taken from 360 sheep sent to slaughterhouses around Van. All serum samples were examined using ELISA for antibodies for Visna Maedi (VMV) and Border Disease (BDV) viruses. Of these, 38 (10.5%) tested positive for Visna Maedi virus antibodies and 163 (45.2%) for Border Disease virus antibodies. Varying numbers of samples were positive for both virus antibodies across the towns of Ercis, Caldiran, Ercek and Baskale in Van, Agri and Hakkari provinces. Both infections should be eliminated by informing veterinarians and animal owners, identifying and eliminating persistently infected animals from flocks, and conducting appropriate eradication measures. Economic support should be provided for this.