Browsing by Author "Karasartova, Djursun"

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Evaluation of Th1/Th2 Balance in Pulmonary Cystic Echinococcosis Patients
(Springer int Publ Ag, 2024) Gazi, Umut; Beyhan, Yunus Emre; Tosun, Ozgur; Karasartova, Djursun; Cobanoglu, Ufuk; Taylan-Ozkan, Aysegul
PurposeCystic echinococcosis (CE) is a neglected tropical disease prevalent worldwide, particularly in rural areas. Previous studies evaluated immune responses in patients with hepatic CE, however none had assessed Th1, Th2 and Th17 levels simultaneously in pulmonary CE patients. This study aimed to fill this gap in literature by using flow cytometry analysis.MethodsPeripheral blood mononuclear cells (PBMCs) were isolated from blood samples collected from healthy control (HC) volunteers and patients with active pulmonary CE cysts. The PBMCs were analysed to evaluate Th1, Th2, and Th17 cell levels within the CD3 + CD4 + T-cell population, using antibodies against interferon (IFN)-gamma, interleukin (IL)-4, and IL-17, respectively.ResultsOur analysis revealed elevated Th2 levels in CE patients, while Th1 and Th17 cell counts showed no significant difference between HC volunteers and patients with pulmonary CE.ConclusionThe results indicate an imbalanced Th1/Th2/Th17 cell regulation in the pathogenesis of pulmonary CE. Future studies are recommended to compare immune responses between pulmonary and hepatic CE to confirm these findings and evaluate any potential difference in the immunopathology associated with the two clinical forms of CE.
Hhv-6 Is Ubiquitously Found Using Western Blot in Tonsils and Adenoid Tissues of Healthy People
(Edizioni int Srl, 2013) Taspinar, Mehmet; Cetin, Nilgun; Gerceker, Devran; Karasartova, Djursun; Turegun, Buse; Ozturk, Sibel; Sahin, Fikret
Few studies have examined the prevalence and cellular proclivity of latent human herpesyirus 6 (HHV-6) in healthy populations. Difficulties in detection of HHV-6 genome in different tissues using polymerase chain reaction (PCR) and immunohistochemistry (IHC) techniques have been reported by various researchers. We examined tonsils and adenoid tissues of 54 patients who had undergone tonsillectomy or adenoidectomy without any evidence of acute infection for the presence of latent HHV-6 infection. While we were investigating the prevalence of HHV-6, we tested the efficiency of PCR, IHC and Western Blot (WB) for detection of HHV-6 in tonsil tissues. We found that 100% of tonsil tissues were positive for HHV-6 with WB, 40% of tonsils were positive with PCR and no tonsil was positive with IHC. This result correlates well with most studies claiming HHV-6 is a ubiquitous organism in various populations and tissues. Western blot may be a good choice for detecting HHV-6 in tissues. Expression of the HHV-6 gp60/110 envelope protein disclosed by WE may indicate that HHV-6 does not have true latency. To our knowledge, this is the first report to use WB to test for HHV-6 in tissues.