Browsing by Author "Karatas, Merve Dilek"

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Effects of Edible Coating (Guar and Tara Gam) Applications on Post-Harvest Fruit Quality and Gene Expressions in Cherry Tomatoes
(Elsevier, 2025) Tekin, Onur; Karatas, Merve Dilek; Cavusoglu, Seyda
Post-harvest storage life of tomatoes (Solanum lycopersicum L.) is relatively short, posing a significant challenge in maintaining fruit quality. Addressing this challenge, our study aimed to evaluate the effects of 1.5 % guar gum and tara gum applications on post-harvest fruit quality and gene expression in Bamano and Yolita cherry tomato varieties cultivated under greenhouse conditions. The tomatoes were stored at 10 degrees C with 80 +/- 5 % relative humidity, and the study assessed changes in respiration rate, external ethylene production, in-package gas composition (O2 and CO2), organic acid content (citric acid, malic acid, succinic acid, tartaric acid, fumaric acid, and oxalic acid), and the expression of genes associated with respiration (LeAOX1a) and ethylene biosynthesis (LeACO1 and LeACS2). The findings revealed that 1.5 % guar and tara gum edible coatings effectively suppressed respiration rates and external ethylene production in both cherry tomato varieties. Additionally, these coatings regulated in-package gas compositions (O2 and CO2) and significantly contributed to the preservation of organic acids. Gene expression analysis indicated that in the Bamano variety, the expression levels of LeAOX1a and LeACS2 increased until the 24th day of storage, while LeACO1 expression levels rise until the 30th day. For the Yolita variety, the expression levels of respiration- and ethylene-related genes (LeAOX1a, LeACS2, and LeACO1) increased until the 24th day. In conclusion, the study demonstrated that 1.5 % guar gum edible coating effectively extended the post-harvest storage life of Bamano cherry tomatoes to 30 days and Yolita cherry tomatoes to 24 days, based on the evaluated parameters. These findings highlight the potential of guar gum coatings as a promising approach for improving the post-harvest quality and storage life of climacteric tomatoes.
Identification of S-Allele Based Self-Incompatibility of Turkish Pear Gene Resources
(Galenos Publ House, 2022) Karatas, Merve Dilek; Hazrati, Nahid; Oguz, Ezgi; Ozmen, Canan Yuksel; Altintas, Serdar; Akcay, M. Emin; Ergul, Ali
Self-incompatibility is considered to be a growth-limiting factor in fruit plants. In species with hermaphrodite flowers, S-locus (S-allele) has been accepted to control self-incompatibility, and the genetic control of this locus is provided by multiple genes (alleles). Pear (Pyrus communis L.) belongs to the Pomoideae from the Rosaceae family and is found to have great genetic potential in terms of ecological features in Turkey. To protect these cultivation features, national garden collections have been established across the country and Ataturk Horticultural Central Research Institute-Yalova collection is considered as genes bank. Identification of the different features of this collection (fruit quality, stress tolerance, self-incompatibility, grafting incompatibility, etc.) is of great importance for its utilization in pear breeding and cultivation. However, to our knowledge, this collection has not been characterized for self-incompatibility trait. In the current study, PCR (Polymerase Chain Reaction)-based amplification of the S-allele regions (S-1, S-6, S-7, S-8) causing the self-incompatibility in 180 pear genotypes obtained from the national pear germplasm was investigated by molecular biological methods based on the comparison of amplified products. In 180 pear genotypes, the S-6 allele was the most prevalent one with 63% frequency, while the S-8 allele was the least common allele with a rate of 4%. In allele combinations, the SI-So allele combination was the most common allele combination with a rate of 18%. and trilateral allele combinations (S-1-S-6-S-7 and S-1-S-6-S-8) were observed at a rate of 1%. Findings of the current research will enable the classification of the materials and the analysed material is likely to be used in breeding studies as well as pear cultivation.
Identification of Self Incompatibility (S) Alleles in Turkish Apple Gene Sources Using Allele-Specific Pcr
(Galenos Publ House, 2023) Karatas, Merve Dilek; Hazrati, Nahid; Yuksel Ozmen, Canan; Hasanzadeh, Mohammad; Altintas, Serdar; Akcay, Mehmet Emin; Ergul, Ali
Self-incompatibility (SI) is a genetic mechanism in many flowering plants by which generative reproduction is prevented. The self-incompatibility caused by the genetic functions of the cell is controlled by genes called S genes or self-incompatibility genes. Self-incompatibility results in decreased pollination and ultimately yield loss. In apple (Malus domestica L.), self-incompatibility is controlled by multi-allelic S-locus. Approaches in the S-glycoprotein profiles and allele-specific PCR methods using the gene profiles and S-glycoprotein profiles for determination of the incompatibility levels are of great importance. In current study, the self-incompatibility status of 192 apple genotypes (such as, Amasya, Huryemez, Sah elmasi, Tokat, Demir elmasi etc.) obtained from the National Collection of Ataturk Horticultural Central Research Institute, Yalova, Turkey, has been determined. For this purpose, genotype-specific allele status and compatibility levels were screened via PCR (Polymerase Chain Reaction) using 4 different S-alleles (Sd, Sf, S26 and S9). 181 genotypes containing at least 1 S-allele were identified as 'Partially Incompatible' and 12 genotypes involving 4 S-alleles were assigned 'Totally Incompatible'. No S-alleles were observed in 2 genotypes (Pancarlik and Huryemez) which exhibited 'Compatibility' status.
Phylogenetic Inference and Secondary Structure Predictions of Turkish Genotypes of Coriandrum Sativum (L.) Based on Its4 and Its5 Nrdna Sequences
(Springer, 2022) Furan, M. Alp; Yildiz, Mehtap; Karatas, Merve Dilek; Chung, Yong Suk
Coriander, Coriandrum sativum L., is an important medicinal plant belonging to the Apiaceae family, which is grown in many parts of Turkey. In this study, seven Turkish coriander varieties (Antakya, Arslan, Erbaa, Gurbuz, Kerkuk, Kudret-K, and Pelmus) were examined for phylogenetic relations using two ITS region (ITS4-ITS5) sequence information and their secondary structure analysis. The length of the nrDNA ITS sequences obtained in this study varied between 677 and 692 nucleotides for seven samples. The G + C content of the nrDNA ITS region ranged between 47.86 (ITS4) and 48.67% (ITS5). Secondary structural analysis revealed that variation between varieties is more unique. The AMOVA study confirmed the effectiveness of configuration analysis as variants accounted for the majority of the variance. In addition, variation within cultivars was significant (97%). This shows that a significant part of the variance detected in this study occurred among genotypes. Data analysis, including Shannon information index and scaled diversity overlap graph annotation, also showed that the highest level of heterozygosity occurred in the Arslan, Erbaa and Kudret-K genotypes, with cultivars showing more pronounced differences genotypically rather than geographically, consistent with the expected heterozygosity (80%). By verification of the PCoA analysis, the genotypes of Gurbuz and Arslan are very different from those of other groups. This supports the results of the cluster analysis, which showed that the Ankara-origin Gurbuz variety had a very different genotypic difference. Our analysis recommends the use of ITS4 sequence and secondary structure data at the intraspecific levels of coriander taxonomical classification. For the first time, a study has used both sequence information and secondary structural information to describe coriander at the varietal level of taxonomic classification.