Browsing by Author "Karatoprak, Gokce Seker"

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Appraisal of the Antimicrobial and Cytotoxic Potentials of Nanoparticles Biosynthesized From the Extracts of Pelargonium Quercetorum Agnew
(Imr Press, 2021) Dumlupinar, Berrak; Karatoprak, Gokce Seker; Firat, Mehmet; Akkol, Esra Kupeli
Aim: The aim of this study is the synthesis of nanosilver particles (AgNPs) from Pelargonium quercetorum Agnew. (Geraniaceae) and evaluation of the antimicrobial and the cytotoxic potential of AgNPs. Methods: The synthesized AgNPs were evaluated for antimicrobial and anticancer efficacy using the minimum inhibition concentration method and MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide) assay. Results: The AgNPs inhibited approximately 90% the growth of gram-positive Staphylococcus aureus and gram-negative Esh-erichia coli and yeast Candida albicans pathogens at a concentration of 500 mu g/mL. The synthesized AgNPs showed excellent toxicity in MCF-7 cells, and specifically, pq70 AgNP inhibited the growth of MCF-7 cells by 52% at a concentration of 3.125 mu g/mL. Conclusion: It was determined that the AgNPs, which had been synthesized from extracts that contained a high phenolic composition, were smaller in size, and showed high anticancer and antimicrobial properties.
Chemical Profile, in Vitro Pharmacological Activity and Satureja Cuneifolia Ten. Evaluation of Essential Oil Based on Distillation Time
(Taylor & Francis Ltd, 2024) Yildiz, Gulsum; Ilgun, Selen; Karatoprak, Gokce Seker; Kose, Yavuz Bulent; Goger, Fatih; Temel, Halide Edip; Demirci, Betul
The medicinal plant Satureja cuneifolia Ten. was widely utilized as spice, tea and traditional medicine. The objective of the current study was to examine the chemical composition and in vitro biological activities (LOX, MMP-1, and MMP-12 enzyme inhibition activity and cytotoxicity on A549 cell line) of Satureja cuneifolia extracts and essential oils. The essential oils of the flowering aerial parts were hydro-distilled at four different distillation times (5, 30, 60, and 180 min) using the Clevenger apparatus. The total essential oil and four fragments were compared in terms of the major component, yield, and distillation time. Volatile compounds of the infusion were extracted by using HS-SPME. Ethanolic extract had the strongest inhibition activity on the LOX enzyme (84.50%), while the essential oils exhibited more cytotoxic activity on the A549 cell line than the extracts. The oils and the infusion were analyzed using GC-MS and the primary chemicals identified by LC-MS/MS.
Phytochemical Composition and Biological Activities of Arctium Minus (Hill) Bernh.: a Potential Candidate as Antioxidant, Enzyme Inhibitor, and Cytotoxic Agent
(Mdpi, 2022) Ilgun, Selen; Karatoprak, Gokce Seker; Polat, Derya Cicek; Safak, Esra Kongul; Yildiz, Gulsum; Akkol, Esra Kupeli; Sobarzo-Sanchez, Eduardo
Arctium minus (Hill) Bernh. (Asteraceae), which has a wide distribution area in Turkey, is a medicinally important plant. Eighty percent methanol extracts of the leaf, flower head, and root parts of A. minus were prepared and their sub-fractions were obtained. Spectrophotometric and chromatographic (high-performance liquid chromatography) techniques were used to assess the phytochemical composition. The extracts were evaluated for antioxidant activity by diphenyl-2-picrylhydrazil radical (DPPH?), 2,2 '-Azino-bis 3-ethylbenzothiazoline-6-sulfonic acid (ABTS(?+)) radical scavenging, and beta-carotene linoleic acid bleaching assays. Furthermore, the extracts were subjected to alpha-amylase, alpha-glucosidase, lipoxygenase, and tyrosinase enzyme inhibition tests. The cytotoxic effects of extracts were investigated on MCF-7 and MDA-MB-231 breast cancer cell lines. The richest extract in terms of phenolic compounds was identified as the ethyl acetate sub-fraction of the root extract (364.37 +/- 7.18 mg(GAE)/g(extact)). Furthermore, chlorogenic acid (8.855 +/- 0.175%) and rutin (8.359 +/- 0.125%) were identified as the primary components in the leaves' ethyl acetate sub-fraction. According to all methods, it was observed that the extracts with the highest antioxidant activity were the flower and leaf ethyl acetate fractions. Additionally, ABTS radical scavenging activity of roots' ethyl acetate sub-fraction (2.51 +/- 0.09 mmol/L Trolox) was observed to be as effective as that of flower and leaf ethyl acetate fractions at 0.5 mg/mL. In the beta-carotene linoleic acid bleaching assay, leaves' methanol extract showed the highest antioxidant capacity (1422.47 +/- 76.85) at 30 min. The enzyme activity data showed that alpha-glucosidase enzyme inhibition of leaf dichloromethane extract was moderately high, with an 87.12 +/- 8.06% inhibition value. Lipoxygenase enzyme inhibition was weakly detected in all sub-fractions. Leaf methanol extract, leaf butanol, and root ethyl acetate sub-fractions showed 99% tyrosinase enzyme inhibition. Finally, it was discovered that dichloromethane extracts of leaves, roots, and flowers had high cytotoxic effects on the MDA-MB-231 cell line, with IC50 values of 21.39 +/- 2.43, 13.41 +/- 2.37, and 10.80 +/- 1.26 mu g/mL, respectively. The evaluation of the plant extracts in terms of several bioactivity tests revealed extremely positive outcomes. The data of this study, in which all parts of the plant were investigated in detail for the first time, offer promising results for future research.