Browsing by Author "Kartal, Deniz Irtem"

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Antiproliferative Activity of Malus Sylvestris Miller Against Hepg2 Cell Line With Their Antioxidant Properties and Phenolic Composition
(Walter de Gruyter Gmbh, 2023) Vuran, Nese Eray; Kartal, Deniz Irtem; Celik, Ismail
Objectives: The purpose of this study was to investigate the in vitro antiproliferative activities of Malus sylvestris Miller (MS) ethanolic and water extracts against HepG2 cells together with their antioxidant and phytochemical profiles.Methods: The extracts' antioxidant capacity was assessed by DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) methods and the total phenolic and flavonoid contents were measured spec-trophotometrically. The antiproliferative activities of ex -tracts were tested via XTT colorimetric assay. The phenolic compounds in the extracts were analyzed using HPLC-DAD analysis.Results: The most abundant phenolic compounds in both extract types were rutin, chlorogenic and gallic acid. In parallel to the phenolic composition, the ethanolic extracts showed the higher antioxidant activity, total phenolic and flavonoid content than the water extracts. The ethanolic extracts displayed 4.5-fold more antiproliferative activity than the water extracts against HepG2 cells. The IC50 values for the ethanolic and water extracts of MS were 0.86 and 3.95 mg/mL, respectively.Conclusions: These results contributed to the understanding of the cytotoxic potential of MS. Since wild apple exhibited strong cytotoxic activity against HepG2 cells, it may be used as a natural agent to treat cancer and the further studies are needed to reveal the effects of wild apple on cancer.
Modulation of Mrna Expression and Activities of Xenobiotic Metabolizing Enzymes, Cyp1a1, Cyp1a2, Cyp2e1, Gpx and Gstp1 by the Salicornia Freitagii Extract in Ht-29 Human Colon Cancer Cells
(inst Bioloska Istrazivanja Sinisa Stankovic, 2017) Altay, Ahmet; Kartal, Deniz Irtem; Sadi, Gokhan; Guray, Tulin; Yaprak, Ahmet Emre
Phase I-II detoxification and antioxidant enzymes are responsible for the detoxification and elimination of activated carcinogens, acting as important biomarkers for chemoprevention. Among them, cytochrome P450s plays a prominent role in the metabolic activation of xenobiotics. The herb Salicornia freitagii (SF) (Amaranthaceae) is known for its anticancer, antioxidant, antidiabetic and antiinflammatory activities. In this study, we determined the bioactive phenolics in the SF methanol extract and investigated its antiproliferative potential in HT-29 human colon cancer cells. We also investigated the modulation of some phase I and II enzyme (CYP 1A1, 1A2, 2E1, GSTP1 and GPx) mRNA expression and enzymatic activities by the SF extract and its major bioactive phenolic compounds. LC/MS-MS analysis showed that the main phenolic compounds of the methanolic SF extract are vanillic acid (48 mu g/100g) and p-coumaric acid (10.8 mu g/100g). SF extract, vanillic acid and p-coumaric acid exhibited high antiproliferative activities in HT-29 cells, with IC50 values of 81.79 mu g/mL, 98.8 mu M and 221.6 mu M, respectively. The mRNA expression levels of CYP1A2 and CYP2E1 were decreased, while those of GSTP1 and GPx in HT-29 cells were increased after application of either the SF extract or vanillic acid. The SF extract by itself also increased the activities of GPx and GSTP1 enzymes 1.68- and 1.49-fold, respectively. Our data indicate that the SF extract and its major bioactive compound, vanillic acid, could exert a modulatory effect on the expression of enzymes that are involved in xenobiotic activation and detoxification pathways in the gastrointestinal tract. For this reason, SF can be considered as a natural source of chemopreventive agents.