Browsing by Author "Kavut, Busra Sumeyye"

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Determination of Il-17, Bcl-3 and Iκbζ Expression Levels in the Gingival Crevicular Fluid of Psoriasis Patients
(Springer, 2025) Talmac, Ayse Gul Oner; Koc, Alaettin; Kavut, Busra Sumeyye
The purpose of this study was to determine the levels of IL-17, Bcl-3 and I kappa B zeta gene expression in the gingival crevicular fluid (GCF) of psoriatic and healthy individuals and to compare the clinical periodontal parameters in the patient and control groups. A total of 10 psoriasis patients and 2 healthy patients in the control group were included in the analysis for IL-17, Bcl-3, and I kappa B zeta gene expression in the GCF. Periodontal health, gingival index, plaque index, and mobility (using a periotest device) levels were compared between the groups. While considering the down-expressed patients, the expression level of the Bcl-3 gene showed the most variability among the genes studied in psoriasis disease. IL-17 gene expression levels were significantly higher and up-regulated in all patients compared to the control group. In addition, when comparing IL-17 with Bcl-3, IL-17 gene expression was notably high in all patients except patients 4, 9 and 10. The highest expression levels of the I kappa B zeta gene were observed in the patient sample, except for patients 7 and 8. While plaque index, gingival index, and pocket depth parameters were higher in the psoriasis group, there was no significant difference between the two groups (p = 0.257, p = 0.390, p = 0.240, respectively). In the periotest (mobility) parameter, while the mean value was higher in the psoriasis group significantly (p = 0.030), clinically mean values of two groups were in the same grade according to Miller mobility index. The I kappa B zeta gene showed notable high expression levels in psoriasis patients. It was concluded that psoriasis may be associated with periodontitis.
Evaluation of the Effects of Andiz Extract on the Experimentally Wound in Rats by Microbiological and Gene Expression Methods
(Springer, 2025) Kavut, Busra Sumeyye; Talmac, Ahmet Cemil; Onalan, Sukru
ObjectivesThis study aimed to investigate the effects of andiz extract on wound healing and compare it with saline and chlorhexidine gluconate. Microbial DNA load was used to evaluate its antibacterial effects, and gene expression methods were used to assess its contribution to cytokine release and wound healing.Methods and ResultsA standardized wound site was created with a 3 mm diameter punch on 32 male Wistar albino rats. The rats were divided into four groups: Control (n = 5), Saline (n = 9), Chlorhexidine gluconate (n = 9), and Andiz extract (n = 9). Five rats in the control group were euthanised without any treatment. Irrigations of the Saline, Chlorhexidine, and Extract groups were provided regularly. After the tissue samples were taken in the 1st week, 2nd week, and 3rd week, three rats were euthanized each week for each group. The total bacterial DNA load on the samples taken was determined by a nano spectrophotometer. beta-actin was chosen as housekeeping, and target gene primers were created for TGF-beta and IL-1 beta. Expression amounts of target genes were measured by Real-Time PCR with the application of the created primers. There is a significant difference between the Extract group and the other groups regarding total bacterial DNA load. The whole bacterial load was 185% less than the initial values. TGF-beta and IL-1 beta genes evaluated regarding gene expression were measured at the highest value in the Extract group.ConclusionsThis study showed the antibacterial effects of the Extract and its positive contributions to wound healing.