Browsing by Author "Kaya, Z."

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The Association Between Platelet-Derived Growth Factor Receptor-Β Polymorphisms and Idiopathic Pulmonary Fibrosis
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Kaya, Z.; Duran, S.; Gunbatar, H.; Sahin, E.S.; Karan, B.M.
Idiopathic pulmonary fibrosis (IPF) is a chronic interstitial lung disease with a dismal prognosis. Platelet-derived growth factor (PDGF) receptor-β (PDGFR-β) are a receptor tyrosine kinase that PDGFs activate. It has been reported that inhibiting PDGFR-β in IPF patients can slow and improve disease progression. However, the effects of PDGFR-β on IPF remain unknown, and no studies on PDGFR-β polymorphisms for IPF have been conducted. The purpose of this study is to look into the relationship between PDGFR-β gene polymorphisms (rs246395, rs2302273, rs3828610, rs138008832) and IPF disease. The study included eleven patients with IPF and twelve healthy controls. DNA was isolated from blood samples taken from all participants, and genotyping was performed using a StepOne plus real time PCR device. There was no statistically significant difference between the variables (age, gender, smoking, alcohol, and gastroesophageal reflux [GER]). There was no statistically significant difference between the patient and control groups in the allele and genotype frequencies of these polymorphisms. Furthermore, no statistically significant difference was found between patients' smoking, forced vital capacity (FVC) (normal, low), and GER data, as well as PDGFR-β variants (rs246395, rs2302273, rs3828610 and rs138008832). In order to determine the relationship between PDGFR-β gene polymorphisms and the risk of IPF, larger studies with more participants are required. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Comparative Molecular Phylogenetics of Astragalus L. Sections From Turkey With New World Astragalus Species Using Nrdna Its Sequences
(2014) Dizkirici, A.; Ekici, M.; Kaya, Z.
Comparative molecular phylogenetic study using nrDNA internal transcribed spacer region (ITS) sequences was carried out on species from three Astragalus L. (Fabaceae) sections from Turkey (Old World species) and the New World. A total of 56 taxa (179 accessions) including 30 species from Incani DC., 15 species from Hypoglottidei DC. and 11 species from Dissitiflori DC. section were used in the current study. The total length of the ITS region was 642 bp with 56 (8.7 %) parsimony-informative and 9 insertion\deletion sites. The constructed phylogenetic tree suggested that the section Incani had the most conserved ITS region among the studied Astragalus sections, evolutionarily separated from other sections and monophyletic. Also, DNA sequences of several New World (Neo-) Astragalus species analyzed comparatively with those from the current study indicated that New World aneuploid species of Astragalus clearly formed a monophyletic group separated from the species of the Old World. © 2013 Springer-Verlag Wien.
Correlation Between the Dna Methylation and Gene Expression of Igfbp5 in Breast Cancer
(IOS Press, 2016) Karabulut, S.; Kaya, Z.; Amuran, G.G.; Peker, I.; Özmen, T.; Gulluolu, B.M.; Akkiprik, M.
BACKGROUND: The insulin-like growth factor binding protein5 (IGFBP5) is often dysregulated in human cancers and considered neither a tumor suppressor nor an oncogene. OBJECTIVE: We aim to examine the reason of the changeable gene regulation of IGFBP5 in the case of methylation in breast cancer. METHODS: We used methyl-specific polymerase (MSP) chain reaction to detect CpG methylation of IGFBP5 promoter and exon-I in breast cancer and adjacent tissues. Gene expression is evaluated by quantative polymerase chain reaction (qPCR). RESULTS: IGFBP5 methylation was detected in 24 of 58 (41%) and 54 of 56 (96.5%) promoter and exon-I site respectively in tumor tissues. In adjacent tissues 17 of 58 (29%) and 53 of 56 (96.5%) was methylated. IGFBP5 expression was higher estrogene receptor (ER)(+) than ER(-) patients (p = 0:0549). Beside, we found a positive correlation between the expression of IGFBP5 and G2 tumor grade (p = 0:0131). However, no correlation was observed between IGFBP5 expression and age, menopause or the presence of lymph node metastasis (p > 0:05). CONCLUSIONS: In summary, our results showed that IGFBP5 promoter and exon-I methylation did not have any differences between tumor and adjacent tissues so that IGFBP5 methylation did not change IGFBP5 gene regulation in breast cancer. This is the first study investigating the IGFBP5 gene methylation in breast cancer. © 2016 - IOS Press and the authors.
Evaluation of Tp53 Codon 72 Polymorphism in Esophageal Cancer Susceptibility in Eastern Anatolia Region of Turkey
(Yuzuncu Yil Universitesi Tip Fakultesi, 2021) Kaya, Z.; Almali, N.; Karan, B.M.; Gorgisen, G.
The tumor suppressor TP53 gene plays a key role in the regulation of cell cycle. Polymorphisms in this gene have been associated with many cancers including esophageal cancer (EC). Many studies in other populations have demonstrated that codon 72 polymorphism of TP53 gene contribute to the prediction of EC risk, especially in Asians. The aim of this study was to explore the effect of codon 72 polymorphism on the EC risk in eastern Turkey. The codon 72 polymorphism was genotyped by real time polymerase chain reaction (qPCR) with TaqMan SNP geno typing assay in 79 patients and 80 healthy control subjects. No statistically significant difference was observed in distribution of genotype and allele frequencies. Heterozygous Arg/Pro (CG) was the most frequent genotype in both patients and controls. Homozygous Arg/Arg (GG) genotype frequency was higher in patients than controls, but not statistically significant (p>0.05). However, tumor location in the lower part of the esophagus was significantly higher in non-C carriers (GG, Arg/Arg) compared to C-carriers (CG/CC) (p=0.01). G-carriers were also more likely to have poorer survival compared to patients with CC genotype (p=0.04). Our results suggest that the Codon 72 polymorphism was not associated with the EC in eastern Turkey. However, GG genotype (Arg/Arg) may have a role in tumor development at the lower location of the esophagus. Additionally, G carriers may exist the poorer survival compared to the non-G carriers (CC). Therefore, it is thought that individuals with CC genotype (Pro/Pro) may have better survival. © 2021, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Impact of Glucocorticoid Receptor Gene (Nr3c1) Polymorphisms in Turkish Patients With Metabolic Syndrome
(Springer, 2016) Kaya, Z.; Caglayan, S.; Akkiprik, M.; Aral, C.; Ozisik, G.; Ozata, M.; Ozer, A.
Background The metabolic syndrome (MetS) is characterized by a cluster of metabolic factors, including insulin resistance and type-2 diabetes, abdominal obesity, dyslipidemia, hypertension and microalbuminuria. Impaired glucocorticoid receptor (GR) activity also plays an important role in the etiology of MetS. The objective of our study is to evaluate the effects of GR gene polymorphisms (BclI, N363S, TthIII1 and ER22/23EK) in Turkish patients with MetS. Materials and methods Seventy subjects with MetS and 185 healthy controls were enrolled in the study. PCR-RFLP analysis was used for genotyping. Results for each polymorphism have been verified by allele-specific oligonucleotide analysis. Results BclI GG genotype was significantly associated with an increased risk of MetS (p = 0.02). Also, only in women, the G allele carriers were significantly associated with higher C-peptide. T allele carriers of TthIII1 polymorphism were significantly associated with higher C-peptide, triglyceride, insulin and C-reactive protein (CRP, p value 0.048, 0.022, 0.005 and 0.022, respectively), and lower fasting blood glucose (FBG, p = 0.02). The combined carriers of BclI polymorphism G allele and TthIII1 polymorphism T allele were significantly associated with higher diastolic blood pressure in all patients, and lower FBG and postprandial blood glucose in only men. All the ER22/23EK polymorphisms coexisted with polymorphic variant of TthIII1 (p = 0.0058). Conclusion The presence of homozygote polymorphic variant of BclI might be good predictive markers for the disease susceptibility. The BclI and the TthIII1 polymorphism are associated with sex-specific clinical parameters. Our findings also suggest that the combination of BclI and TthIII1 polymorphisms may play a protective role in blood glucose.
Squamous Cell Carcinoma of the Lower Lip and Supra-Omohyoid Neck Dissection
(ARSMB-KVBMG, 2003) Kutluhan, A.; Kiriş, M.; Kaya, Z.; Kisli, E.; Yurttaş, V.; Içli, M.; Käsem, M.
Purpose: The aim of this study is to evaluate our approach to patients with squamous cell carcinoma of lower lip. Patients and methods: This study includes 31 lower lip squamous cell carcinomas followed up between 1994 and 2000. Primary treatment was applied to 28 patients of whom 23 were in stages I-II and five in stages III-IV. Three patients presented locoregional recurrence. Neck dissection was performed during primary lip resection in patients with palpable cervical lymph node involvement. Patients with unpalpable cervical lymph nodes were divided into two subgroups: one was submitted to elective neck dissection (n = 11) and the other had isolated lip resection (n = 8). Unilateral or bilateral selective supra-omohyoid neck dissection (SOHND) was performed according to the localisation of the disease. Radical dissection was performed in a secondary intervention, when SOHND revealed lymph node metastases. Radiotherapy and chemotherapy were applied for curative and / or adjuvant treatment in addition to surgery in patients with locoregional recurrence and metastatic lymph nodes or with perineural involvement. Results: Occult cervical metastasis within a single lymph node was found in one of the 11 No patients who underwent elective neck dissection. Delayed neck metastasis developed in one of the eight patients in whom isolated lip resection (without neck exploration) was performed. Chemoradiotherapy was administered to this patient, but he died. Neck metastasis was established histologically in four of five patients in stages III-IV. Postoperative radiotherapy was used on these patients. One of the patients in this group died due to inoperable local recurrence in the neck, another died because of distant metastasis. Local mandibular recurrence was seen in one of these patients after three years. Comment: Six patients (19%) died due to lower lip carcinoma in this series. Our findings show the importance of elective neck dissection and intact surgical resection margins.