Browsing by Author "Kazak, Filiz"

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Immunoglobulins and Acute Phase Proteins in Van Cats - Associations With Sex, Age, and Eye Colour
(Tubitak Scientific & Technological Research Council Turkey, 2021) Coskun, Pinar; Altunok, Vahdettin; Kazak, Filiz; Yuksek, Nazmi
It was aimed to determine concentrations of plasma immunoglobulins (IgG, IgA, IgM) and acute phase proteins (alpha-1 acid glycoprotein, serum amyloid A, ceruloplasmin) and the association of these parameters with age, sex, and eye colour in Van cats. Blood plasma of healthy, forty-seven Van cats (Van Cat Home) fed with standard cat food were involved in the study. Cats were divided into four groups based on age (<1, 2 to 2.5, 3 to 4, > 5) and eye colour (amber-amber, amber-blue, blue-amber, and blue-blue eyes described from left to right), and two groups based on sex (male and female). Plasma IgG, IgA, IgM, alpha-1 acid glycoprotein, serum amyloid A, and ceruloplasmin concentrations were determined, and the concentrations were found to be 2.20 +/- 0.05 mg/mL, 1.05 +/- 0.04 mg/mL, 2.52 +/- 0.18 mg/mL, 562.00 +/- 14.27 mu g/mL, 1.49 +/- 0.03 mu g/mL, and 2.88 +/- 0.13 mg/dL, respectively. Male Van cats had higher IgA levels than the female cats (P < 0.05). alpha-1 acid glycoprotein concentrations in cats under 1-year-old were higher from that in the other age groups, whereas the significant difference was obtained compared to 2 to 2.5-year-old cats (p < 0.05). IgM concentrations were higher in cats with blue-blue eye colour than in the cats with other eye colours, while statistically significant difference was observed only cats with amber-blue eye colour (p < 0.05). Amber-amber eyed cat plasma ceruloplasmin concentrations were higher from that in the other eye coloured cats while significant difference was determined compared to amber-blue eyed cats (p < 0.05). Plasma immunoglobulins and acute phase proteins were determined and the associations of these parameters with age, sex, and eye colour were examined for the first time in Van cats. These data are thought to be important as reference levels in terms of diagnosis and prognosis.
Nobiletin Alleviates Methotrexate-Induced Hepatorenal Toxicity in Rats
(Taylor & Francis Ltd, 2024) Kazak, Filiz; Uyar, Ahmet; Coskun, Pinar; Yaman, Turan
We investigated the possible ameliorative effects of nobiletin (NBL) against methotrexate (MTX)-induced hepatorenal toxicity in rats. Twenty-eight Wistar albino rats were randomly divided into four groups, namely: Control; MTX (administered 20 mg/kg MTX); MTX+NBL (administered 20 mg/kg MTX and 10 mg/kg NBL per day); and NBL (administered 10 mg/kg/day NBL). Histopathological, immunohistochemical and biochemical analyses were performed on the kidney and liver tissues of rats at the end of the study. MTX caused renal toxicity, as indicated by increases in malondialdehyde (MDA) and caspase-3, as well as decreases in reduced glutathione (GSH), glucose-6-phosphate dehydrogenase (G6PD), glutathione peroxidase (GPx), catalase (CAT) and B-cell lymphoma-2 (Bcl-2). MTX also caused hepatotoxicity, as indicated by increases in 8-hydroxy-2'-deoxyguanosine (8-OHdG), tumor necrosis factor alpha (TNF-alpha), MDA and caspase-3 and decrease in interleukin 10 (IL-10), GSH, total antioxidant capacity, GPx, G6PD, CAT and Bcl-2. MTX caused histopathological changes in kidney and liver tissues indicating tissue and cellular damage. Administration of NBL concurrently with methotrexate reduced oxidative stress, inflammatory and apoptotic signs, and prevented kidney and liver damage caused by methotrexate. We consider NBL has attenuating and ameliorating effects on methotrexate-induced hepatorenal toxicity.