Browsing by Author "Keleş, Ö.F."

Now showing 1 - 4 of 4

The Effect of Tarantula Cubensis (Theranekron) on Some Reproductive Parameters in Male Rats
(University of Tehran, Faculty of Veterinary Medicine, 2023) Koşal, V.; Keleş, Ö.F.
Background: Tarantula cubensis (theranekron), obtained from the poison of Cuban Tarantula, is a homeopathic drug frequently used in animal health. Objectives: The effect of theranekron on reproductive parameters in male animals is unknown. In this study, the effect of theranekron on male reproductive parameters was investigated. Methods: Three different groups of rats were created: control (n=8) (no application), theranekron I (n=8) (subcuteneous injection of 0.3 mg/kg on days 0, 5, and 10), and theranekron II (n=8) (subcuteneous injection of 0.3 mg/kg on days 0, 5, and 10). Control and theranekron I groups were sacrificed at the end of the 4 weeks, and the theranekron II group was sacrificed at the end of the 6 weeks. Sperm motility, density, abnormal rate, DNA damage, and testicular histopathology were examined. Results: No statistical difference was observed in the parameters examined between the study groups (P>0.05). It was determined that the theranekron did not change sperm motility, density and spermatozoa ratio, also no histopathological changes, and sperm DNA damage. Conclusion: There is no harm in using theranekron in male animals during the breeding and nonbreeding seasons. Copyright © 2023 by Prusa Medical Publishing.
Effect of Urtica Dioica Seed Extract on Aquaporin 1 and 7, Caspase-3 and Oxidant/Antioxidant Status in Diethylnitrosamine-Induced Kidney Damage in Rats
(Yuzuncu Yil Universitesi Tip Fakultesi, 2024) Keleş, Ö.F.; Huyut, Z.; Yıldızhan, K.; Demir, A.
Diethylnitrosamine (DENA) is known to have a carcinogenic effect on the liver by stimulating oxid ative stress and inflammation. There are few studies on the negative effects of DENA on kidney tissue, and the therapeutic effect of Urtica dioica Seed Extract (UDSE) against the negative effects of DENA is investigated for the first time in this study. Th is study investigated the protective effect of UDSE on kidney tissues of rats administered DENA. The rats were divided into four groups (n=8): control, UDSE (1 mL/kg/daily for 16 weeks), DENA (200 mg/kg/first day), DENA+UDSE (200 mg/kg/first-day single dose, and 1 mL/kg/daily for 16 weeks). At the end of the experiment, blood and kidney tissues were taken for biochemical and pathological analysis. DENA administration has been shown to increase oxidative stress in the kidneys and reduce antioxidant levels. Moreover, compared to the DENA group, DENA decreased the level of aquaporin (AQP)-1 in kidney tissue, while UDSE treatment increased both AQP-1 levels in kidney tissue and AQP-1 and 7 levels in the serum samples. Histopathological examination of the kidney revealed significant coagulation necrosis, especially in proximal tubular epithelial cells, hyperemia in capillaries, mononuclear cell infiltration between tubular area s, atrophy in the glomerular cluster, and adhesions with Bowman's capsule. In addition, there was a decrease in both caspase-3 (immunohistochemically) expression and TOS levels in the DENA+UDSE group compared to the DENA group, while an increase was seen in antioxidant levels. These findings indicated that UDSE may be an essential therapeutic agent against DENA-induced kidney injury. © 2024, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Investigation of Histopathological and Biochemical Changes in Rats Feeding Roasted and Raw Sunflower (Helianthus Annuus L.) Seed Extract
(Yuzuncu Yil Universitesi Tip Fakultesi, 2023) Sinan, M.T.; Huyut, Z.; Yıldızhan, K.; Keleş, Ö.F.; Yener, Z.
Today, sunflower seeds, which are in first place in the consumption of nuts, have many benefits. However, sunflower seeds are consumed mainly by roasting. In this study, the effect of roasted and raw sunflower seeds on the liver of rats fed with experimentally roasted and raw sunflower seeds was investigated histopathologically and biochemically. For this purpose, a total of 24 rats were with eight rats in each group; the control, roasted sunflower seed (ROSS), and raw sunflower seed (RASS) groups. At the end of the experiment, blood and tissue samples were taken from the rats for histopathological and biochemical examinations. While degenerative-necrotic changes were detected in the livers of rats fed with roasted sunflower seeds, no significant morphological changes were detect ed in the raw sunflower and control groups. Furthermore, there was no significant difference between the groups in the biochemi cal parameters AST, ALT, CAT, GSH, BcL-2 and SOD. However, TAS levels in the ROSS group were significantly lower than in the other groups, while LDH, Bax and caspase-3 levels were high. Data showed that ROSS decreased antioxidant status in liver tissue and increased LDH levels compared to RASS and could damage liver tissue. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.
Protective Effect of Subsieve Grape Extract (Vitis Vinifera L.) on Testes, Reproductive Hormones, and Some Spermatological Parameters in Heat Stress-Induced Rats
(Ataturk Universitesi, 2023) Koşal, V.; Avci, A.; Bingöl, N.T.; Keleş, Ö.F.; Kömüroğlu, A.U.; Okulmuş, Ç.
Heat stress (HS) has negative effects on reproductive parameters in male animals. This study was designed to investigate the protective effects of Vitis vinifera L., which has a natural antioxi-dant property, on reproductive parameters, testicular oxidative stress, reproductive hormones, and testicular histopathology in male rats treated with HS. Fifty male rats were divided into 5 different groups (control, HS, HS + 100, HS + 200, and HS + 300). It was determined that subsieve V. vinifera extract given at different doses eliminated the negative effects of HS administration on reproductive parameters. It was determined that V. vinifera increased sperm motility and density and decreased the rate of abnormal sperm regardless of dose (P <.001). It was determined that increased oxidative stress and decreased luteinizing hormone (LH), follicle-stimulating hormone (FSH), and testosterone levels as a result of HS administration improved in the extract-treated groups (P <.05), and FSH and testosterone levels increased in the HS + 200 and HS + 300 groups compared to the control group (P <.001). Histopathologically, it was determined that the degen-eration, edema, and congestion caused by HS disappeared due to the extract administration. As a result of this study, the protective effect of subsieve V. vinifera extract on HS on male reproductive parameters was revealed. © Author(s).