Browsing by Author "Keleş, Omer Faruk"

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Effect of Different Doses of Transdermal Fentanyl on the Reproductive System in Male Rats
(Pakistan Journal of Pharmaceutical Sciences, 2025) Koşal, Volkan; Kurt, Nurettin; Okulmus, Caglar; Keleş, Omer Faruk
Fentanyl, a potent synthetic opioid analgesic, is commonly used to manage severe pain. However, the effects of fentanyl use on male reproductive health have not been adequately studied. This study aimed to investigate the effects of different doses of transdermal fentanyl patches on various reproductive parameters in male rats. Adult male Albino Wistar rats were divided into four groups. The treatment groups received transdermal fentanyl at doses of 25 mcg/h (Group II), 50 mcg/h (Group III), and 100 mcg/h (Group IV) for 9 days, respectively. Sperm motility, sperm concentration, abnormal sperm, live/dead sperm, testicular apoptosis, testicular oxidative stress, and androgen receptor levels were evaluated. The results showed that fentanyl administration decreased the oxidative stress parameters CAT and SOD1 levels in all treatment groups (p<0.001). No significant changes were observed in sperm motility, abnormal sperm ratio, or live/dead sperm ratio. However, Group IV showed a significant increase in sperm concentration compared to the other groups (p<0.001). In addition, all fentanyl treatment groups showed a significant increase in apoptosis-related Caspase 3/8/9 enzymes (p<0.001). This study reveals the effects of fentanyl on male reproductive health. This is the first study to demonstrate an increase in sperm concentration associated with high fentanyl doses. © 2025 Elsevier B.V., All rights reserved.
Selenium Reduces Cadmium-Induced Cardiotoxicity by Modulating Oxidative Stress and the ROS/PARP-1/TRPM2 Signalling Pathway in Rats
(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Yazğan, Yener; Keleş, Omer Faruk; Bayir, Mehmet Hafit; Çi̇çek, Hacı Ahmet; Ahlatci, Adem; Yıldızhan, Kenan
Cadmium (CAD) is a prevalent environmental contaminant that poses serious cardiotoxic risks. The heart, kidney, liver, and brain are just a few of the essential organs that can sustain serious harm from CAD, a very poisonous heavy metal. The cardiotoxic mechanism of CAD is linked to oxidative damage and inflammation. A trace element with anti-inflammatory, anti-apoptotic, and antioxidant qualities, selenium (SEL) can be taken as a dietary supplement. The biotoxicity of heavy metal CAD is significantly inhibited by SEL, a mineral that is vital to human and animal nutrition. Through ROS-induced PARP-1/ADPR/TRPM2 pathways, this study seeks to assess the preventive benefits of selenium against cardiovascular damage caused by CAD. The SEL showed encouraging results in reducing inflammatory and oxidative reactions. Rats were given 0.5 mg/kg SEL and 3 mg/kg 2-Aminoethyl diphenylborinate (2-APB) intraperitoneally for five days, in addition to 25 mg/kg CAD given via gavage. Histopathological examination findings revealed that the morphologic changes in the hearts of the CAD group rats were characterised by marked necrosis and the degeneration of myocytes and congestion of vessels. Compared to the rats in the CAD group, the hearts of the SEL, 2-APB and SEL+2-APB groups showed fewer morphological alterations. Moreover, in rats given CAD, there was an increase in cardiac malondialdehyde (MDA), total oxidant (TOS), reactive oxygen species (ROS), caspase (Casp-3-9), and TNF-α, whereas glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and total antioxidant (TAS) decreased. SEL improved antioxidants, avoided tissue damage, and reduced cardiac MDA, TOS, and ROS. In rats given CAD, SEL decreased cardiac PARP-1, TRPM2, TNF-α, and caspase. In summary, by reducing oxidative stress and cardiac damage and modifying the ROS/PARP-1/TRPM2 pathway, SEL protected against CAD cardiotoxicity. © 2025 Elsevier B.V., All rights reserved.