Browsing by Author "Khawar, K.M."
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Article Effects of Plant Growth Regulators on Lentil (Lens Culinaris Medik.) Cultivars(Bangladesh Botanical Society, 2015) Ozdemir, F.A.; Turker, M.; Khawar, K.M.Lentil (Lens culinaris Medik.) is an annual pulse legume crop of immense economic importance. This study reports use of 16 lentil cultivars' shoot tips, internode, hypocotyl, cotyledon and root explants cultured on MS medium containing 3 mg/l BAP-0.5 mg/l 2,4-D or 0.25 mg/l IBA for shoot regeneration. The results showed maximum shoot regeneration from cv. Yesil 21 on internode explants on 3mg/l BAP-0.5 mg/l 2,4-D. Best shoot regeneration on MS medium containing 0.25 mg/l IBA was noted on internode explants of cv. Seyran 96. In general shoot regeneration on 0.25 mg/l IBA was better compared to 3mg/l BAP and 0.5 mg/l2,4-D. Well developed shoots from all cultivars regenerated on both types of culture media were rooted on MS medium containing 0.19 mg/l NAA. A system to regenerate lentil is established that will help in easy genetic transformation in future.Article Evidence of Efficient in Vitro Micropropagation From Bulb Scales of Starch Grape Hyacinth (Muscari Neglectum Guss. Ex. Ten.)(Serbian Genetics Society, 2024) Fi̇Da, A.; Keskin, N.; Kahriz, P.P.; Kahriz, M.P.; Khawar, K.M.Muscari neglectum is a widespread plant species that grows in various areas of the Mediterranean region in the wild. The species has a high potential for use as cut flowers, outdoor or indoor ornamental or garden plants. It is also used as a food, and forage plant on limited scales in rural areas. There is a lack of commercial production protocols for M. neglectum. The development of a fast and efficient protocol for commercial production, may contribute to the economy of Türkiye and improve local people’s quality of life. Therefore, this study aimed to develop safe in vitro vegetative propagation techniques using 2, 3, 4, and 5 bulb scale explants on MS medium using 1 mg BAP L-1 + 0.00, 0.40, 0.60, 0.80, 1.00, 1.20 mg NAA L-1. Any number of bulb scale explants obtained from freshly harvested bulbs were not suitable for micropropagation and developed necrosis. However, any number of bulb scales obtained from 6 weeks of waiting for bulbs did not show the problem. A regeneration percentage of 13.33 to 100% was noted on 2-scale explants. Additionally, 100% callus formation was observed on these scales using MS medium containing 1 mg BAP L-1 + 0.8, 1.0, and 1.20 mg NAA L-1 (3 combinations). In addition, 1.93 bulblets with a diameter of 0.97 cm (the largest) were observed on MS medium containing 1 mg BAP L-1 + 0.4 mg NAA L-1. Although, 3, 4, and 5 bulb scale explants induced bulblets in variable numbers, the rate of bulblet induction was not at par with bulblet induction on 2 scale explants. The largest bulblets developed on 2-scale explants were rooted on ½ × MS medium containing 0.5 mg NAA L-1. The results obtained for micropropagation of M. neglectum species are of great importance for the commercial production of the species. © (2024), (Serbian Genetics Society). All rights reserved.