Browsing by Author "Kilinc, Ozlem Orunc"

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Chewing Lice (Phthiraptera: Amblycera, Ischnocera) on Several Species of Wild Birds Around the Lake Van Basin, Van, Eastern Turkey
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Goz, Yasar; Dik, Bilal; Kilinc, Ozlem Orunc; Yilmaz, Ali Bilgin; Aslan, Logman
This study was performed to detect chewing lice on the wild birds in Eastern Turkey, between April 2013-September 2014. 108 injured birds brought to Wild Animal Protection Center of Yuzuncu Yil University were examined for louse. The feathers of each bird specimens were inspected for louse, macroscopically. Collected lice samples on the birds were preserved in 70% ethyl alcohol and mounted on slides in Canada balsam after transparented in 10% KOH. Fifteen (14.95%) out of the 108 were found to be infested with at least one chewing louse species. Nineteen lice species in 15 genera were found on the infested birds. Goniocotes megalocephalus (Uchida, 1916) on the Helmeted Guineafowl (Numida meleagris); Actornithophilus piceus lari (Packard, 1870) on the Armenian Gull (Larus armenicus); Kurodaia fulvofasciata (Piaget, 1880) on the Long-legged Buzzard (Buteo rufinus); Laemobothrion sp. on the Golden Eagle (Aquila chrysaetos); Trinoton anserinum (Fabricius [J.C.], 1805) and Holomenopon sp. on the Greylag Goose (Anser anser) were recorded for the first time from Turkey in this study.
Cryptosporidium Spp. in Dogs - Prevalence and Genotype Distribution
(Univ Fed Rio Grande Do Sul, 2023) Celik, Ozgur Yasar; Kochan, Akin; Celik, Burcak Aslan; Ayan, Adnan; Akyildiz, Gurkan; Kilinc, Ozlem Orunc; Ayan, Ozge Oktay
Background: Cryptosporidium spp. is a zoonotic protozoan parasite that affects the gastrointestinal tract of humans and animals. The disease can cause acute and chronic diarrhoea and even death in both humans and animals. In this study, it was aimed to determine the prevalence and genotype distribution of Cryptosporidiosis in shelter dogs in Diyarbakir province located in the Southeastern Anatolia Region of Turkey. Materials, Methods & Results: The animal material of the study consisted of 100 dogs of different breeds and sexes. Faecal samples were collected from the rectum with disposable latex gloves and placed in individual sample containers. All of the samples were examined for Cryptosporidium spp. by Kinyoun Acid Fast and Nested PCR methods. In the Kinyoun Acid Fast staining method, firstly, smear preparations were prepared from fresh faecal samples, fixed in pure methanol for 1 min and allowed to dry. The slides were kept in Kinyoun Carbol-Fuxin for 5 min, dipped in 50% ethyl alcohol, shaken, washed in tap water, kept in 1% sulphuric acid for 2 min and washed in tap water. The slides were kept in methylene blue for 1 min, washed in tap water and allowed to dry. After drying, immersion oil was dripped and examined under a microscope at 100 magnification. DNA extraction was performed from all samples using GeneMATRIX Stool DNA Purification Kit according to the manufacturer's protocol. After Nested PCR analysis was performed. In the PCR step, primers 5'-TTCTAGAGCTAATACATGCG-3' and 5'- CCCATTTCCTTCCTTCGAAACAGGA-3' were used to amplify the 1325 bp gene region. In the nested PCR step, primers 5'- GGAAGGGTTGTATTTATTTATTAGATAAAG-3' and 5'-AAGGAG-TAAGGAACAACCTCCA-3' were used to amplify the 826-864 bp gene region. As a result of both methods, a prevalence of 3% was determined. The infection rate was higher in males (3.57%) than females (2.27%) and in younger than 1 year (5.56%) than in older than 1 year (1.56%). The DNA sequences obtained from the sequence analysis of 3 positive PCR samples were analysed in BioEdit software. A phylogenetic tree was constructed with the data set created by using the 18s rRNA gene sequences obtained from the NCBI genbank database and the DNA sequences obtained as a result of the study, and it was shown which Cryptosporidium species the study samples were related to. Today, many Cryptosporidium species have been identified and most of these species have host adaptation. Although C. canis is the most common species in dogs, C. muris, C. meleagridis, and C. parvum have also been detected. Among these species, C. parvum is recognized as a zoonotic species infecting a wide range of mammals. In this study, DNA sequencing of nested PCR positive samples revealed that 3 samples were zoonotic C. parvum. Discussion: This suggests that dogs may be a reservoir for zoonotic transmission of Cryptosporidium. Consequently, it is recommended that people should be informed about the potential for transmission of this protozoan to humans and animals and that control programmes should be implemented, including the prevention of free entry of stray dogs into public places and homes.
Detection of Cryptosporidium Spp. in Calves Through Nested Pcr and Kinyoun's Acid-Fast Methods in Van, Turkey
(Health & Environment Assoc, 2020) Ayan, Adnan; Kilinc, Ozlem Orunc; Yuksek, Nazmi; Basbugan, Yildiray
Cryptosporidium is a protozoon of zoonotic importance causing infections with diarrhoea and other clinical symptoms in man, domestic, and wild animals. The present study was conducted to determine the prevalence of cryptosporidiosis in diarrhoeic calves which were up to 1 month old in Van, Turkey, using Kinyoun's acid-fast and Nested PCR methods. In this study A total of 164 diarrhoeic calves faecal samples were collected from diarrheic calves at different farms located in Van, Turkey during January 2019 to September 2019. The faecal samples stained with Kinyoun's acid-fast method then examined with microscope. DNAs were extracted from all the faecal samples using RTA stool DNA isolation kit. In order to amplify the SSU rRNA gene region of the DNAs, appropriate primers were used in the Nested PCR. According to the results, In the microscopic examination, Cryptosporidium spp. oocyst were identified in 61 out of 164 diarrhoeic calves faecal samples (37.19%). Cryptosporidium spp. specific bands of 826-864 bp in size were obtained from 72 out of 164 diarrhoeic calves faecal samples (43.9%). To the best of our knowledge, no other previous study is available with concern to molecular investigation of the prevalence of Cryptosporidium in calves around Van, Turkey. With this study, it was revealed that cryptosporidiosis is an important agent in calf diarrhoea, and measures should be taken in this regard.
Determination of Cryptosporidium Spp. in Van Cats by Nested Pcr
(Polish Soc veterinary Sciences Editorial office, 2018) Kilinc, Ozlem Orunc; Yilmaz, Ali Bilgin; Goz, Yasar; Ozkan, Cumali; Denizhan, Vural
The present study was conducted to investigate the presence of Cryptosporidium spp. agents in cats from the Turkish Van Cat Shelter at YuzuncuYil University by a modified Ziehl-Neelsen staining method and nested PCR. Individual stool samples were obtained from 30 adult females, 30 adult males and 40 kittens - a total of 100 Van cats were analyzed in the study. A simplified formol-ether concentration method was applied to all samples. The samples were then examined microscopically by a modified Ziehl-Neelsen staining method. As a result of the staining, Cryptosporidium spp. oocysts were identified in stool samples of 3 kittens in the microscopic examination. After that, PCR and nested-PCR were conducted with suitable primers. Nested PCR identified 5 kittens (5%) as positive. As a result, it was concluded that nested PCR was a superior diagnostic method for Cryptosporidium diagnosis compared with staining methods and that infected cats could be a health hazard for other cats and individuals, since Cryptosporidium spp. agents infect via the faecal-oral route. Therefore, we believe it is necessary to raise the awareness of people in contact with cats.
Evaluation of Oxidant, Antioxidant, Interleukin and Biochemical Parameters in Sheep With Cystic Echinococcosis and Dicrocoeliosis
(Polish Soc veterinary Sciences Editorial office, 2025) Ertas, Fatma; Komuroglu, Ahmet Ufuk; Kilinc, Ozlem Orunc; Basbugan, Yildiray; Yuksek, Nazmi
Aim: Endoparasitic infections have long posed a significant threat to the sheep industry, leading to substantial economic losses. Among these parasites, Dicrocoelium dendriticum and, particularly, Echinococcus granulosus are globally important zoonotic parasites. This study aimed to investigate the levels of oxidative stress markers, antioxidant parameters, interleukins, and biochemical indicators, as well as their correlations, in sheep affected by cystic echinococcosis and dicrocoeliosis. Methods: In this study, various biochemical and immune-related parameters were assessed. Serum samples collected from infected sheep were analyzed using ELISA to measure interleukin-18 (IL-18), interleukin-10 (IL-10), interleukin-6 (IL-6), interleukin-4 (IL-4), NGAL, neopterin, myeloperoxidase, SOD, GPX, catalase, GSH, and MDA. Additionally, spectrometric analysis was performed to determine the levels of AST, ALT, GGT, albumin, globulin, total protein, ALP, and iron. Results: Serum MDA levels were found to be significantly elevated in infected sheep compared to the healthy group. In contrast, serum SOD and GSH levels in infected sheep were significantly lower than those in healthy sheep. Additionally, serum AST and ALT activities were markedly higher in the infected group. Interleukin levels showed no significant differences between the two groups. Overall, markers of liver function and oxidative stress were notably increased in infected sheep. Conclusion: It was concluded that oxidative stress and liver damage occurred in the infected group, and their immune system was actively involved in the response. This study not only adds to academic knowledge, but should also help veterinarians and breeders working in the field to make more accurate decisions. Thus it is expected to contribute directly to herd health, animal welfare, economic efficiency, and public health. Correlation data may also be useful in guiding the treatment process.
First Report of Zoonotic Cryptosporidium Parvum Subtype Iiaa15g2r1 in Dogs in Türkiye
(Univ Agriculture, Fac veterinary Science, 2024) Ayan, Adnan; Celik, Burcak Aslan; Celik, Ozgur Yasar; Akyildiz, Gurkan; Kilinc, Ozlem Orunc; Ayan, Ozge Oktay; Uslu, Ugur
Cryptosporidium (C.) is an opportunistic protozoan causing gastrointestinal illness in both humans and animals, leading to acute or chronic diarrhea and even death. The study aimed to investigate the prevalence and subtyping of Cryptosporidium spp. in shelter dogs in Van province, T & uuml;rkiye. For microscopic identification of this parasite, a total of 300 fecal samples were collected and stained with Kinyoun's acid-fast method. For molecular analysis, the positive samples were subjected to DNA extraction and SSU rRNA gene of Cryptosporidium spp. was amplified using nested PCR. The microscopic examination revealed a 4.67% prevalence of Cryptosporidium spp. Sequence analysis indicated all samples were positive to C. parvum. In addition, GP60 gene was also amplified and C. parvum subtypes IIaA15G2R1 was confirmed by analyzing the obtained sequences. All the sequences of SSU rRNA and GP60 were deposited in GenBank. To our knowledge, Cryptosporidium parvum subtypes IIaA15G2R1 have been reported first time in dogs in T & uuml;rkiye. It is recommended to implement control strategies by awareness campaign, preventing stray dogs from freely entering public areas, and proper disposal of dog feces.
First Reports of Sarconema Eurycerca and Trinoton Anserinum in the Whooper Swan (Cygnus Cygnus) in Van, Turkey
(Kafkas Univ, veteriner Fakultesi dergisi, 2015) Oguz, Bekir; Kilinc, Ozlem Orunc; Deger, M. Serdar
Whooper swan Cygnus cygnus (Linnaeus, 1758) with wounded wing that found in the Ercis district of Van province was brought into Directorship of Wild Animal Protection of University of Yuzuncu Yil. Despite the surgical interventions, the whooper swan could not be rescued. Five lice in the feather and three nematodes in the heart were found at examination of the whooper swan. These lice specimens were identified as Trinoton anserinum (Fabricius, 1805). After the necropsy, nematodes were found in the heart of the whooper swan. Nematodes were identified as Sarconema eurycerca according to their morphological pecularities. Sarconema eurycerca have been reported for the first time in Whooper swan in Van, Turkey.
The Investigation of Giardiasis (Foodborne and Waterborne Diseases) in Buffaloes in Van Region, Turkiye: First Molecular Report of Giardia Duodenalis Assemblage B From Buffaloes
(Mdpi, 2023) Kilinc, Ozlem Orunc; Ayan, Adnan; Celik, Burcak Aslan; Celik, Ozgur Yasar; Yuksek, Nazmi; Akyildiz, Gurkan; Oguz, Fatma Ertas
Giardia duodenalis (G. duodenalis) is an important zoonotic protozoan agent that causes foodborne and waterborne diarrhea in humans and other mammals. Molecular-based tests are critical in diagnosing giardiasis in humans and animals, identifying species, understanding the zoonotic potential and transmission routes, and evaluating taxonomy. Therefore, this study aimed to investigate the molecular characterization of G. duodenalis in buffaloes in the Van region in Turkiye. Buffaloes are a species that has been poorly studied in this regard. For this purpose, 100 fecal samples were collected from buffaloes in the Van region. The DNA extraction was performed using the GeneMATRIX STOOL DNA Purification Kit from stool samples. The nested PCR test was performed with the appropriate primers from the obtained DNA samples. The obtained bands suitable for sequencing were sent for sequence analysis, and the sequence results were aligned bidirectionally and compared with the database of GenBank by BLAST. As a result of the study, an 11% positivity rate for G. duodenalis was found in buffaloes, and assemblage E and assemblage B were isolated. To our knowledge, assemblage B in buffaloes was reported for the first time in this study. As a result, it was concluded that buffaloes are an important reservoir for waterborne and foodborne giardiasis.
Investigation of Toxoplasma Gondii and Neospora Caninum in Different Tissues of Aborted Foetuses of Sheep in Van Province, Turkiye: Analysis by Nested Pcr, Histopathological and Immunohistochemical Methods
(veterinarni A Farmaceuticka Univerzita Brno, 2023) Kilinc, Ozlem Orunc; Ayan, Adnan; Yumusak, Nihat; Kömüroglu, Ahmet Ufuk; Aslan, Burcak; Celik, Ozgur Yasar; Göz, Yasar
Toxoplasma gondii and Neospora caninum are protozoon parasites from the intracellular apicomplexan family. Toxoplasma gondii is the cause of health and economic problems in the sheep industry worldwide. Neospora caninum is usually reported in cows and leads to infections causing abortions; however, its prevalence in sheep is not clear. The present study aimed to investigate the prevalence and pathology of T. gondii and N. caninum by PCR, histopathological and immunehistochemical methods in aborted sheep foetuses collected at different sheep flocks in the Van Province, Turkiye, in 2021. Firstly, the DNA of T. gondii and N. caninum were investigated by PCR in the brain, heart, and peritoneal fluid samples from 42 sheep foetuses. Toxoplasma gondii DNA was proved in 35.7% (15/42) of foetuses whereas N. caninum DNA was not determined in any of the samples. Histopathologically, all T. gondii positive brain tissue samples showed lymphohistiocytic multifocal encephalomyelitis and additional findings included necrotizing myocarditis in the positive heart samples. Toxoplasma gondii tachyzoites were identified in the lesions (diffuse or focal mononuclear cell infiltration in the meninges, and microglia proliferation, myocarditis with oedema) by anti-T. gondii antibodies by the immunohistochemical method. Based on our results, we can conclude that T. gondii is an important agent in sheep abortions and the PCR method is a suitable method for diagnosis which can also be used in heart tissue in pathological studies.
Molecular Characterization of Tetratrichomonas Gallinarum in Domestic Turkeys (Meleagris Gallopavo) in Van Province, Turkey
(Health & Environment Assoc, 2020) Ayan, Adnan; Kilinc, Ozlem Orunc; Khan, Jamal Muhammad; Yilmaz, Ali Bilgin; Karakus, Ayse Sona; Bia, Mohammed Mebarek; Lee, Dongmin
Tetratrichomonas gallinarum is a protozoan parasite of various galliform birds causing a type of enterohepatitis termed tetratrichomoniasis. During the year 2018-2019 an outbreak of unspecified granulomatous diseases, characterized by the sudden deaths of young turkeys were observed in 18 turkey farms in the Van province of Turkey. Three hundred eighty fecal samples were obtained from the infected farms and 42 tissue samples (liver and caecum) collected from the dead turkeys were examined under light microscope and through molecular characterization. The necropsies of dead turkeys revealed granulomatous necrotic foci in the caecum and liver. A number of 125 (32.89%) fecal samples and all of the 42 (100%) tissue samples were found positive for T. gallinarum by molecular characterization. The sequence analysis for both fecal and tissue samples were confirmed with T. gallinarum (MH935510) those showed 97.9% similarity on the 'Basic Local Alignment Search Tool'. This is the first molecular characterization report of T. gallinarum in Turkey.
Molecular Detection of Mycoplasma Haemocanis and Candidatus Mycoplasma Haematoparvum in Rhipicephalus Sanguine Us Tick Species Collected From Dogs in Adana Province of Turkey
(Parlar Scientific Publications (p S P), 2021) Sababoglu, Ezgi; Ayanz, Adnan; Kilinc, Ozlem Orunc; Yilmaz, Ali Bilgin; Tekindal, Mustafa Agah; Akkaya, Hayrettin; Celik, Burcak Aslan
Mlycoplasma haemocanis (Mhc) and Candidatus Mycoplasma haematoparvum (CMhp) arc hemo-tropic mycoplasma species known to be specific to dogs. Rhipicephalus sanguineus (sensu lam) ticks have been suggested to bc the main vector in transmission; but a clear relationship between tick infestation and the disease has yet to be established under field conditions. This study aimed to investigate the presence of Mhc and CMhp in R. sanguineus (s.1.) s.ticks that arc considered to be a possible vector for hemoplasma infections, Using the species -specific polymerase chain reaction (PCR) test, the presence of Mhc. and CMhp was investigated in total of 312 archived adult tick samples collected from 63 pet dogs kept in the municipal dog shelter of Adana province located in the Mediterranean region of Turkey. Although R. sanguineus (s.1.) ticks tested negative for Mhc, 2.56% (n = 8) were positive for CMhp. There was a statistically significant difference in the Mhc and CMhp detection rates in R. sanguineus (s.1.) ticks (2.56%; 95% CI: 0.81 - 4.31; P < 0.05). To our knowledge, this is the first study to reveal the presence of CAlhp in R. sanguineus (s.l.) ticks collected from dogs in Turkey.
Molecular Detection of Neospora Caninum From Naturally Infected Dogs in Van Province, East Turkey
(Agricultural Research Communication Centre, 2020) Yilmaz, Ali Bilgin; Goz, Yasar; Kilinc, Ozlem Orunc; Denizhan, Vural
Neospora caninum is a coccidian parasite causing abortion in cattle and neurological problems in horses. Dogs are definitive hosts of N. caninum. Polymerase chain reaction is the most specific method used for the detection of N. caninum oocytes. In the present study, a total of 100 fecal samples were collected from naturally infected dogs. Of the 100 samples analyzed, 11 of them were detected with Hammondia/Neospora-like oocytes. Genomic DNA was isolated using a commercially available DNA extraction kit. The Nc5 gene specific to N. caninum was amplified by PCR and two of the eleven samples with Hammondia/Neospora-like oocytes formed similar to 337 bp repeatable band. In conclusion, N. caninum, which has been shown to cause neurological disorders in dogs and to abortion in cattle, was detected in naturally infected dogs in Van Province in Polymerase chain reaction.
Molecular Identification of Ehrlichia Canis in Rhipicephalus Sanguineus Ticks From Siirt Province
(Sciendo, 2021) celik, Burcak Aslan; Ayan, Adnan; Yilmaz, Ali Bilgin; celik, Ozgur Yasar; Kilinc, Ozlem Orunc; Ayan, Ozge Oktay
This study was performed on Ehrlichia canis positive ticks collected from dogs to perform sequencing of their 16S rRNA genetic section using the PCR method. The collection of ticks was performed from a total of 60 dogs in the Siirt province, Turkey. A total of 250 ticks were collected and morphologically investigated. All ticks were identified as Rhipicephalus sanguineus sensu lato (s.l). Ehrlichial DNA was detected by the PCR method performed on 38 (15.2 %) of the ticks. The E. canis strains obtained as a result of the sequence analysis were found to be 100% identical to the American Texas (MH620194), Indian (KX766395), and Egyptian (MG564254) strains. This study thereby has identified a zoonotic agent from the R. sanguineus ticks collected from the dogs in the Siirt province.
Molecular Identification of Hepatozoon Canis in Ticks From Dogs in Siirt, Turkey
(Natl information Documentation Centre, 2022) Celik, Burcak Aslan; Ayan, Adnan; Yilmaz, Ali Bilgin; Celik, Ozgur Yasar; Kilinc, Ozlem Orunc; Ayan, Ozge Oktay
HEPATAZOON species are tick borne protozoan parasites classified in the Heptazoidae family, and they are closely related to hemosporinids and piroplasms. In this work, the 18S rRNA genetic section of Hepatozoon canispositive ticks removed from dogs was sequenced using the PCR technique. Ticks were collected from a total of 80 dogs in Siirt, Turkey.A total of 300 collected ticks were morphologically identified to the species level and all ticks identified as Rhipicephalussanguineus (s.l.). H. canis DNA was detected in 12 (%4) out of 300 in R. sanguineusticks by PCR. The phylogenetic tree created via comparison of amplified 18S rRNA region sequences of H. caniswith MT107097.1, MH595911.1, KT215377.1, KT 215376.1, KC 584780.1, KC 584777.1, KC 584775.1, and KC 584774.1. The results obtained will provide important reference material for both veterinary cliniciansand dog owners in terms of managing canine hepatozoonosis.
A Molecular Survey of Hepatozoon Canis in Dogs in the Siirt Province of Turkey
(veterinarni A Farmaceuticka Univerzita Brno, 2022) Celik, Burcak Aslan; Celik, Ozgur Yasar; Ayan, Adnan; Yilmaz, Ali Bilgin; Kilinc, Ozlem Orunc; Ayan, Ozge Oktay
This study aimed to determine Hepatozoon canis prevalence in dogs in the Siirt province of Turkey by the molecular method. The animal material of the study consisted of a total of 75 dogs that appeared clinically healthy. Two ml of blood sample were taken from the vena cephalica antebrachii. Then, DNA extraction was performed. Polymerase chain reaction (PCR) was performed to amplify the 666 bp 18S rRNA gene region of Hepatozoon canis. Two positive PCR products were purified and sequenced. As a result of Nested-PCR, H. canis specific bands in 666 bp size were obtained in 7 (9.33%) out of 75 dogs. The result of sequence analysis, the nucleotide sequence was registered in the NCBI GenBank database with accession numbers OL467380.1-OL467538.1. Hepatozoon canis registered in GenBank of sequence OL467380.1 was found to be similar with other H. canis strains of registration numbers MW684292.1 with 99.69% and MH615006.1-MK091085.1-MF797806.1 with 99.53% rates; and the sequence with registration number OL467538.1 was found to be similar to the series MW684291.1 with 99.09% and MH615006.1-MK091085.1-KX 818220.1 with 99.08% rates by BLAST analysis. Hepatozoon canis prevalence of dogs in the Siirt province was determined as a result of this study. It is of great importance to take preventive measures, especially to fight ticks with appropriate acaricides, since there is no vaccine to prevent the disease.
Preliminary Studies on the Prevalence and Genotyping of Echinococcus Granulosus Infection in Stray Dogs in Van Province, Turkey
(Walter de Gruyter Gmbh, 2018) Oguz, Bekir; Ozdal, Nalan; Kilinc, Ozlem Orunc; Deger, M. Serdar
Introduction: Echinococcus granulosus is a zoonotic helminth of the Taeniidae family living in the small intestines of dogs. The hydatid cyst, which is the larval form of this parasite, is observed in sheep, goat, cattle, and many other organisms including humans. It causes a disease called cystic echinococcosis. Identification of strains of E. granulosus in dogs is critical in parasite control and eradication where possible. This study aims to determine the genotype of E. granulosus eggs and prevalence of this parasite in the faeces of dogs in the Van Province using the copro-PCR method. Material and Methods: This study was conducted between 2015 and 2016 on the faeces obtained from 100 stray dogs from different parts of the Van Province. The coprological examination was conducted using the formalin-ether concentration method. Results: Taeniidae eggs were found in 10 (10%) out of 100 faecal samples. E. granulosus was detected in 4 out of 10 of these (40%) infected samples. Sequence analysis of positive amplicons obtained from PCR showed that there were sheep strains (G1). Conclusion: Dogs in Van area are primarily infected with the livestock genotype of E. granulosus, which is thought to be a potential zoonotic threat to humans.
Prevalance and Molecular Characterization of Giardia Duodenalis in Livestock in Van, Turkey
(Health & Environment Assoc, 2019) Ayan, Adnan; Ural, Deniz Alic; Erdogan, Hasan; Kilinc, Ozlem Orunc; Gultekin, Mehmet; Ural, Kerem
Giardia duodenalis (Giardia lambia, Giardia intestinalis), a flagellated parasite, is a gastrointestinal pathogen that affects public health worldwide. There are limited studies on the epidemiology and molecular characterization of G. duodenalis in farm animals in Turkey. Molecular characterization is requited to determine the zoonotic potential of Giardia infection. In the present study, 71 calf 50 lamb, and 66 goat fecal stool samples were obtained from the animal rectum using a disposable latex glove and the samples were transferred to the laboratory. Then, the presence of cysts was examined with a microscope using of saturated zinc sulfate flotation method (ZnSO4; 33%). Afterwards, the samples were scanned with ELISA-based rapid diagnostic test kits for diagnosis. Then, DNA was extracted with QIAamp DNA Stool Mini Kit (Qiagen, Germany). Nested PCR was then conducted with the corresponding primers. DNA sequence analysis was conducted on beta-giardin gene region of each PCR positive samples. Then, sequence analyses were blasted and compared with the access numbers in the gene bank (M36728 for sub-genotype A1, AY072723 for sub-genotype A2, for AY072724 sub-genotype A3, AY072725 for subgenotype B1, AY072726 for sub-genotype B2, AY072727 for sub-genotype B3, AY072728 for sub-genotype B4). In the microscopic examination, Giardia cysts were identified in 41 calves, 18 lambs, and 23 goats. Positivity was determined with rapid test kits in 38 calves, 16 lambs, and 20 goats. In nested PCR results, specific bands (511 bp) were obtained in 46 calves, 21 lambs and 24 goats. Sequencing findings demonstrated Assemblage A and subgenotype A3 presence in all animal samples. The importance of treatment and the need to take protective measures due to its zoonotic importance were emphasized.
Prevalence and Genotypes of Giardia Duodenalis in Shelter Dogs of Southeastern Türkiye
(Urmia Univ, 2023) Celik, Burcak Aslan; Celik, Ozgur Yasar; Kochan, Akin; Ayan, Adnan; Kilinc, Ozlem Orunc; Akyildiz, Guerkan; Ercan, Kerem
Giardia duodenalis is a protozoan parasite found in humans and several mammals. This parasite spreads worldwide and is generally recognized as a zoonotic agent being reported to be one of the most common causes of diarrhea in humans and animals. In this study, it was aimed to determine the prevalence and genotypes of G. duodenalis in shelter dogs in Diyarbakir province being located in the southeastern Anatolia region of Turkiye. Native-Lugol method and nested polymerase chain reaction analyses of 100 fecal samples showed a prevalence of 3.00 and 4.00%, respectively. The prevalence was higher in females and in those younger than 1 year. Sequence analysis revealed the presence of zoonotic assemblage B, assemblage D and assemblage E. The detection of zoonotic assemblage B in this study suggests that dogs may be a reservoir for human giardiasis. Further molecular research is needed to determine the genotype diversity of Giardia as well as its possible role in the transmission of this parasite to humans.(c) 2023 Urmia University. All rights reserved.
Prevalence and Molecular Characterization of Cryptosporidium Spp. in Calves in the Siirt Province, Turkiye
(veterinarni A Farmaceuticka Univerzita Brno, 2023) Celik, Ozgur Yasar; Sahin, Tekin; Celik, Burcak Aslan; Kilinc, Ozlem Orunc; Ayan, Adnan; Akyildiz, Gurkan; Gormez, Gul
Cryptosporidiosis, one of the main protozoan infections of the last century, is especially dangerous for calves and causes significant economic losses. This research was carried out to determine the prevalence of Cryptosporidium spp. by microscopic and molecular methods and to determine subtypes in 100 calves up to 6 months old and with diarrhoea in the Siirt Province, Turkiye. As a result of the microscopic examination (Kinyoun's acid-fast), Cryptosporidium spp. oocysts were found in 8 (8%) of 100 samples. As a result of nested PCR, 826-864 bp specific bands for Cryptosporidium spp. were obtained in 13 (13%) of 100 samples. When the DNA sequences of the SSU rRNA gene were compared with the NCBI Basic Local Alignment Search Tool database, it was determined that eight samples sequence analyses showed 100% similarity with the C. parvum, C. ryanae, and C. bovis samples. The detection of C. parvum, which has zoonotic importance in this study, suggests that calves with diarrhoea may be a source of contamination for other animals and humans. Therefore, animal owners and people in close contact with animals should be informed about the public health of cryptosporidiosis.
Prevalence and Subtypes Distribution (St10, St14, St25, St26) of Blastocystis Spp. in Anatolian Water Buffalo (Bubalus Bubalis) in Van, Turkiye
(Wiley, 2024) Ayan, Adnan; Celik, Burcak Aslan; Celik, Ozgur Yasar; Yilmaz, Ali Bilgin; Kilinc, Ozlem Orunc; Ayan, Ozge Oktay
Background: Blastocystis spp. is one of the most common protozoa worldwide, living in the gastrointestinal tract of humans and many other animals. On the basis of the genetic heterogeneity of small subunit ribosomal RNA, at least 28 subtypes (ST1-ST17, ST21 and ST23-ST32) are reported to exist in mammals and birds. ObjectivesThis study was carried out to determine the prevalence and subtypes of Blastocystis spp. in Anatolian buffaloes (Bubalus bubalis) in Van province in the Eastern Anatolia Region of Turkey. Methods: DNA was extracted using commercial GeneMATRIX Stool DNA Purification Kit and then stored at -20 degrees C until PCR amplification. After PCR amplification of the SSU rRNA gene region positive Blastocystis spp., amplicons from buffalo faeces were sequenced and then deposited in GenBank (OR576949.1, OR576950.1, OR576970.1, OR576971.1, OR577019.1, PP837943.1, PP837940.1, PP837939.1, PP837604.1, PP837937.1, PP837934.1, PP837601.1, PP837936.1 and PP837603.1). Results: PCR analysis of 120 faecal samples showed a total prevalence of 11.67% (14/120). The prevalence was higher in females and young animals (p > 0.05). Sequence analysis revealed Blastocystis spp., ST10, ST14, ST25 and ST26 subtypes. To our knowledge, Blastocystis subtypes ST25 and ST26 in buffaloes were reported for the first time in this study. Conclusion: It is thought that more large-scale studies should be carried out to determine the zoonotic subtype potential of this protozoan in the region.