Browsing by Author "Kiraz, Selahaddin"

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Detection of Putative Loci Affecting Milk Yield in Turkish Awassi Sheep Using Microsatellite Markers
(Springer, 2024) Koncagul, Seyrani; Kiraz, Selahaddin; Koyun, Hasan
On the basis of comparisons between bovine and ovine genome mapping information, the aim of the study was to analyze the genetic diversity of selected DNA microsatellites from the bovine genome and to investigate their correlation with the average daily milk yield in Awassi sheep. 18 informative microsatellite markers were selected from the significant QTL regions affecting milk yield identified in the bovine genome in previous studies. The selected microsatellite markers were then amplified by PCR as reciprocal amplifications on the genomic DNA of Awassi sheep, with standard daily milk yield records. Thus, in this study, 18 microsatellite markers associated with milk yield in the bovine genome were examined for both determination of genetic polymorphism within the flock and the effects of marker loci on average daily milk yield in Awassi sheep. Allele frequencies of markers were determined based on the results of fragment analysis. The analysis of variance showed that the 123 bp allele at the marker locus BMS1341 on BTA2 significantly influenced the average daily milk yield of Ivesi sheep (P < 0.01). On the other hand, the BMS381 locus with a 115 bp allele on BTA2, the MCM140 locus with a 185 bp allele on BTA6, the BMS2721 locus with a 155 bp allele, the BM1237 locus with 174 and 180 bp alleles on BTA7, and finally, the BMS1967 locus with a 117 bp allele, the BM4208 locus with 176 and 182 bp alleles, and the INRA locus with a185 bp allele on BTA8 showed moderately significant effects on the average daily milk yield of Ivesi ewes (P < 0.05).
A Mitochondrial Dna-Based Molecular Phylogenetics Study of the Mahalli Goat as a New Animal Genetic Resource in Southern Anatolia in Turkey
(Agricultural Research Communication Centre, 2024) Kiraz, Selahaddin; Koncaguel, Seyrani; Vural, Mehmet Emin; Koyun, Hasan
Background: The Karacada & gbreve; Mountains are of extinct volcanic origin and cover a large area in the triangle between Diyarbak & imath;r (Center, Nar and Ergani districts), & Scedil;anl & imath;lurfa (Siverek and Viransehir districts) and Mardin (Derik district). Agricultural activities are limited due to the stony structure of the region; therefore, small animal husbandry is intensively practiced in the villages of the region. Mahalli goats, which are morphologically new domestic goats, are distinguished from other domestic goats by their spiral horn structure. They are not only resistant to cold weather conditions, but also resistant to diseases. Therefore, present study aimed to determine the genetic diversity and phylogenetic relationships of Mahalli goats in Diyarbak & imath;r region. Methods: The study was carried out at & Scedil;anl & imath;urfa, Harran University of Agricultural Faculty, Laboratory of Animal Biotechnology and Molecular Genetics, in 2019-2020. Out of 24 Mahalli goats, forward and reverse primers were used to amplify the 598 bp region over the mtDNA D-loop region encompassing the 481 bp most variable region (HVR1). Analysis of goat D-loop sequences revealed 481 regions of 0.378 G+C, 97 polymorphic regions and 19 haplotypes. The phylogenetic analysis of Mahalli goats was carried out using molecular genetic techniques. Result: As a result of data analysis, both haplotype diversity (HD) and nucleotide diversity (ND) were calculated to be 1.000 +/- 0.0039 and 0.0375 +/- 0.00209, respectively. The genetic distance between the haplotypes of Mahalli goat ranged from 0.003 to 0.087 and the genetic distance between the haplotypes of Mahalli goat and other domestic goat breeds in Turkey ranged from 0.016 to 0.027. All 19 haplotypes used in this study were represented in the lineage A phylogenetic tree.
Molecular Genetic Analysis With Rapd-Pcr Method of Zom Sheep
(2014) Vural, Mehmet Emin; Bingöl, Mehmet; Kiraz, Selahaddin
Bu çalışmada 2012-2014 yılları arasında Diyarbakır İli Karacadağ yöresinde, farklı sürülerde bulunan 18 baş zom koyunundan alınan kan örnekleri kullanılmıştır. Harran Üniversitesi Ziraat Fakültesi Hayvansal Biyoteknoloji Labaratuvarı'nda izole edilen DNA örnekleri 8 adet RAPD primeri ile kullanılarak işlenmiş ve RAPD-PCR bantları elde edilmiştir. Agaroz- Jel fotoğrafları incelenmiş ve elde edilen bantlar ile bantların var (1) ve yok (0) olmasına göre oluşturulan veri matrisi, PopGen32 paket programı kullanılarak, değerlendirilmiştir. Çalışmada 300-3000 bç aralığında 899 adet bant elde edilmiştir. Kullanılan 8 adet primer ile toplamda 54 lokus tespit edilmiş olup, bu lokusların 15'i polimorfik 39'u monomorfik bulunmuş, polimorfizm oranı ise % 27.8 olarak hesaplanmıştır. OPA-19 primeri ile tüm lokuslar polimorfik bulunurken, OPE-02 ve OPE-06 primerlerinde ise polimorfizm tespit edilememiştir. Genetik uzaklık, en yüksek 3 – 6 (0.189) nolu örnekler arasında, en düşük ise 4 - 5 (0.019) nolu örnekler arasında tespit edilmiştir. Genetik benzerlik değeri en az 3 - 6 nolu örnekler arasında (0.811), en çok ise 4 - 5 nolu örnekler arasında (0.981) bulunmuştur. Ortalama heterozigotluk 0.1253 olarak bulunmuştur. Sonuç olarak RAPD-PCR yöntemiyle Zom koyunlarında polimorfizm, genetik benzerlik, genetik uzaklık ve heterozigotluk gibi bazı genetik parametreler hesaplanarak DNA seviyesinde tanımlayıcı ön bilgiler elde edilmiş, bireylerin heterojen bir yapıda olduğu ve bu genetik çeşitliliğin ıslah amaçlı kullanım potansiyeline sahip olduğu söylenebilir.
Relationships Between Growth Characteristics and Gdf9 Gene Polymorphisms in Awassi Sheep
(Taylor & Francis Ltd, 2024) Kiraz, Selahaddin; Koncagul, Seyrani; Koyun, Hasan
The Awassi sheep, a breed with a distinctive fat tail, is bred specifically in the south-eastern region in Turkey. It has proven its adaptability to the hot environment of this region, especially in the provinces of & Scedil;anl & imath;urfa, Hatay and Gaziantep, which are close to the Syrian border. The aim of this study was to investigate the SNP polymorphisms in the GDF9 gene/exon2 using DNA sequencing. In addition, the study investigated the relationships between SNP genotypes and growth traits, in particular birth weight (BW), weaning weight (WW) and weight at the end of the grazing season (GSW), in Awassi sheep. Four different flocks with a total of 131 Awassi lambs were used. PCR was used to amplify exon2 of the GDF9 gene region, which has a length of 815 base pairs (bp), from the genomic DNA of the Awassi sheep. Thus, four new single nucleotide polymorphisms (SNPs) were identified. The results showed that although the effects of SNP2 and SNP4 on body weight (BW) were statistically significant, only SNP4 had a significant effect on weaning weight (WW) (P < 0.05). In addition, SNP1 and SNP3 were found to have no significant effect on any developmental trait in the dataset.
Single Nucleotide Polymorphisms of Gdf9 Gene/Exon 2 Region and Their Associations With Milk Yield and Milk Content Traits in Karakas and Norduz Sheep Breeds
(Tubitak Scientific & Technological Research Council Turkey, 2021) Koyun, Hasan; Kiraz, Selahaddin; Karaca, Serhat; Koncagul, Seyrani; Yilmaz, Ayhan; Karakus, Kadir; Aygun, Turgut
Karakas and Norduz sheep have been adapted to the Lake Van region of Turkey for many years as being domestic genetic resources and meat, milk, and fleece traits are a reliable economic resource especially preferred by small family businesses around the area. In this study, the data set consisted of milk yield (MY) and milk content components; milk fat (MF), fat-free dry matter (FFDM), dry matter (DM), protein (Pro), lactose (Lac), pH, acidity H (aH), and lactic acid (Lac). Besides, Karakas (n = 30) and Norduz (n = 26) sheep were chosen to investigate based on the SNP method. Correspondingly, genomic DNA from both breeds exon 2 of the GDF9 gene region was amplified, 815 base pairs (bp) in length, by means of PCR. Therefore, there were three noval SNPs detected in both breeds under investigation. Although SNP1 and 2 with genotypes of GG and AG had statistically significant impacts on both milk production and milk components (p < 0.01), SNP3 with genotypes of TT, TC had no significant effects on the milk characteristics in question.