Browsing by Author "Kizildag, Sibel"

Now showing 1 - 2 of 2

Effect of Tlr10 (2322a/G, 720a/C, and 992t/A) Polymorphisms on the Pathogenesis of Crimean Congo Hemorrhagic Fever Disease
(Wiley, 2018) Kizildag, Sibel; Arslan, Serdal; Ozbilum, Nil; Engin, Aynur; Bakir, Mehmet
Crimean Congo hemorrhagic fever (CCHF) is a tick-borne disease caused by the Crimean Congo hemorrhagic fever virus (CCHFV). Toll-like receptors (TLRs) are type 1 transmembrane proteins of immune cells that play a critical role in innate and adaptive immunity. The present study first time aims to investigate the relation between TLR10 gene polymorphisms (720A/C, 992T/A, and 2322A/G), severity/non-severity, fatality/non-fatality, and CCFH disease by using PCR-RFLP assay in a Turkish population. TLR10 720A/C polymorphism was determined to be statistically significant both genotype and allele frequency (P=0,011, P=0.015, respectively). TLR10 992T/A polymorphism was found statistically significant relationships between patient and control (P=0.026) and individual with AA genotype have approximately three times greater risk than TT genotype (OR=2.93). There was not a significant difference in 2322A/G genotype distribution (P=0.152). There were also statistically significant associations between both TLR10 992T/A and 2322A/G polymorphism and patient mortality (P=0.001 and P=0.008, respectively). We have not found statistically any linkage among TLR10 haplotype, but individual AAA and GAT haplotype have higher risk than individual AAT haplotype (OR=3.22, OR=1.93, respectively). Consequently, this study shows that pathogenesis of CCHF disease is associated with the TLR10 720A/C and 992T/A polymorphisms. There is a statistically significant association in fatal/non-fatal patients with TLR10 720A/C and 992T/A. The TLR10 992AA genotype might increase and TLR10 720CC genotype might decrease susceptibility to pathogenesis of CCHF disease. TLR 10 polymorphisms may be also an important biomarker for CCHF susceptibility and fatality rate.
Morphology and Molecular Phylogeny of Four Frontonia Species From Turkey (Protista, Ciliophora)
(Magnolia Press, 2019) Kizildag, Sibel; Yildiz, Ismail
In this study, Frontonia leucas, Frontonia acuminata, Frontonia angusta, and Frontonia anatolica species isolated from aquatic environments of Van in Turkey were investigated in detail using morphological, morphometrical, and molecular methods. Although there were minor differences, the Frontonia populations were morphologically similar to the other previously reported populations of the 4 species. Frontonia leucas differed from the other populations by the following combination of characters: about 200 somatic and only 3 vestibular kineties, and a single micronucleus. The Turkish population of Frontonia acuminata had just 4 vestibular kineties and the large micronucleus was always located to the anterior of the carrot-shaped macronucleus. The ratio of the oral apparey size to the cell size of Frontonia angusta in this study was about 18%, with 3 vestibular kineties, and 1 excretory pore. Phylogenetic trees based on small-subunit rRNA gene sequences were constructed using Bayesian inference and maximum-likelihood. Frontonia anatolica was more closely related to Apofrontonia dohrni and Paramecium spp. than to its congeners, while F. acuminata, like F. terricola, was also more closely related to the family Stokesiidae. The results indicated that Frontonia is a non-monophyletic genus consisting of 3 groups. We presented the systematic relationships of the genera and families of Peniculida with new data of genus Frontonia herein.